Article
Oncology
Hector Katifelis, Maria-Paraskevi Nikou, Iuliia Mukha, Nadiia Vityuk, Nefeli Lagopati, Christina Piperi, Ammad Ahmad Farooqi, Natassa Pippa, Efstathios P. Efstathopoulos, Maria Gazouli
Summary: Nanoparticles composed of a gold and silver alloy can induce multiple cell death pathways, including necroptosis and pyroptosis, which have promising anticancer effects. These nanoparticles also trigger the release of danger signals, which may play a beneficial role in cancer.
Article
Integrative & Complementary Medicine
Lucas dos Santos Zamarioli, Michele Rosana Maia Santos, Adolfo Garcia Erustes, Vanessa Marques Meccatti, Thais Cristine Pereira, Soraya S. Smaili, Maria Cristina Marcucci, Carlos Rocha Oliveira, Gustavo J. S. Pereira, Claudia Bincoletto
Summary: This study evaluated the effects of Artemisia vulgaris (AV) hydroalcoholic extract (HEAV) on breast cancer cells, chronic myeloid leukemia cells, and fibroblasts. The results showed that HEAV had cytotoxic effects on these cells, primarily inducing cell death through apoptosis and necrosis, partly dependent on lysosomal release of calcium.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2023)
Article
Cell Biology
Maria Moros, Eugenio Fergola, Valentina Marchesano, Margherita Mutarelli, Giuseppina Tommasini, Beata Miedziak, Giuliana Palumbo, Alfredo Ambrosone, Angela Tino, Claudia Tortiglione
Summary: Extracellular vesicles (EVs) play a crucial role in cell-cell communication in aquatic environments, protecting signals from degradation and coordinating physiological processes. Analysis of EVs from Hydra vulgaris revealed various markers, including stress-induced messages and regulators of the Wnt/beta-catenin signaling pathway. These findings suggest a bioactive cargo in EVs and provide insights into developmental signaling mechanisms across species in aquatic ecosystems.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Marina Simon, Jesper Tranekjaer Jorgensen, Kamilla Norregaard, Jonas Rosager Henriksen, Gael Clergeaud, Thomas L. Andresen, Anders Elias Hansen, Andreas Kjaer
Summary: Traditional cancer treatments are often insufficient to achieve complete responses, so combination therapies are commonly used. Nanoparticle-based photothermal therapy (PTT) is a promising add-on treatment that uses localized hyperthermia to kill cancer cells. In this study, we evaluated the combination of gold nanoshell-based PTT and liposomal doxorubicin for colorectal cancer treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Review
Nanoscience & Nanotechnology
Anqi Zhang, Lei Gao
Summary: Radiotherapy is an important method for treating malignant tumors. Enhancing its therapeutic gain ratio through physical techniques is the current direction of radiotherapy. The combination of hyperthermia and radiotherapy can improve the radiosensitivity of tumor cells and has significant clinical value. Nanomaterials have attracted widespread attention in tumor hyperthermia research and show great potential. This article summarizes the sensitizing mechanisms and effects of gold nanotechnology and provides an overview of the advancements of other nanoparticles in radiation therapy.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Rui Cai, Meng Wang, Meiyuan Liu, Xiongjie Zhu, Longbao Feng, Zhongjian Yu, Xia Yang, Zhiwu Zhang, Huili Guo, Rui Guo, Yanfang Zheng
Summary: The study confirmed that high expression of CDK7 promotes an immunosuppressive macrophage phenotype and macrophage infiltration in lung adenocarcinoma (LUAD). Researchers developed an AuNP/siCDK7 system to activate the antitumor immune response and improve the treatment of LUAD.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Lorenzo Torrisi, Letteria Silipigni, Lubomir Kovacik, Vasily Lavrentiev, Mariapompea Cutroneo, Alfio Torrisi, Laura Maria De Plano, Domenico Franco, Salvatore Guglielmino
Summary: The study focuses on the uptake of gold nanoparticles by M13 phages and their potential application in transporting heavy metallic nanoparticles into tumor cells for improved DNA destruction efficiency during radiotherapy and hyperthermia treatments. The presence of gold nanoparticles was found to enhance the imaging contrast of the phages.
APPLIED SCIENCES-BASEL
(2021)
Review
Chemistry, Multidisciplinary
Song Shen, Jichuan Qiu, Da Huo, Younan Xia
Summary: Photothermal therapy shows great potential in killing cancer cells through hyperthermia. The efficacy is affected by various parameters, including the laser, photothermal transducers, spatial distribution of the transducers, bioeffect of the tissue (heat-sink effect, thermal conductivity), and real-time temperature monitoring.
Article
Nanoscience & Nanotechnology
Somia Tomane, Claire Wilhelm, Souhir Boujday, Alexandre Fromain, Antoine Miche, Flavien Bourdreux, Anne Dolbecq, Pierre Mialane, Anne Vallee
Summary: Monodisperse and perfectly shaped core/shell AuNP@Mo(4)Zol(2)Mn nanohybrids were designed as excellent candidates for combined chemotherapy and photothermal cancer therapy. The nanoparticles were readily confined in endosomal compartments of prostate cancer cells and showed significant anticancer activity under photothermal treatment. This study demonstrates the potential of AuNPs capped by POMs in cancer therapy.
ACS APPLIED NANO MATERIALS
(2021)
Review
Biochemical Research Methods
Jiang Liu, Lijun Ma, Guoyang Zhang, Yuzhi Chen, Zhuo Wang
Summary: Surface modification of nanomaterials is a critical strategy to enhance their capability in scavenging ROS, which possesses inherent antioxidant properties and significant biological implications.
BIOCONJUGATE CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Nandan Gupta, Rishabha Malviya
Summary: The use of gold nanoparticles in photothermal therapy for cancer treatment is highlighted in this communication, showing their potential in warning cancer cells to other therapies, modulating cellular permeability and delivery, and achieving effective therapy management.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Environmental Sciences
Jae Soon Kang, June-Woo Park
Summary: The acute toxicity of Ag2S-NPs was low in algae, crustaceans, and fish, but significantly restored in cnidarians. This restoration of toxicity in cnidarians may be caused by Ag+ dissolved in the gastrovascular cavity under acidic conditions.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Polymer Science
Aftab Alam, Talha Jawaid, Saud M. Alsanad, Mehnaz Kamal, Pinki Rawat, Vinita Singh, Pravej Alam, Prawez Alam
Summary: This study developed a transdermal nanoformulation for Proteus vulgaris using hesperidin, which showed good drug release and antimicrobial activity. It could be an advanced approach for treating Proteus vulgaris infections.
Article
Engineering, Chemical
Xi Gu, Darson D. Li, Guan H. Yeoh, Robert A. Taylor, Victoria Timchenko
Summary: This study found that localized heat generation of GNP aggregates in acidic solutions can be significantly enhanced (up to 60.0 degrees C) when illuminated by a near infrared light source at 1.7 W/cm².
Article
Chemistry, Multidisciplinary
Udesh Dhawan, Ching-Li Tseng, Huey-Yuan Wang, Shin-Yun Hsu, Meng-Tsan Tsai, Ren-Jei Chung
Summary: Cell behavior is influenced by nanomaterial geometry, with core-shell cobalt-gold nanoparticles in spherical and elliptical morphology showing potential applications in cancer theranostics.
Article
Biochemical Research Methods
Jaromir Behal, Daniele Pirone, Daniele Sirico, Vittorio Bianco, Martina Mugnano, Danila del Giudice, Beatrice Cavina, Ivana Kurelac, Pasquale Memmolo, Lisa Miccio, Pietro Ferraro
Summary: Live cells can be used as optical components in bio-hybrid systems, and various cell types have been studied for this purpose. However, the optical properties of White Blood Cells (WBCs) have not been investigated yet. In this study, we accurately analyze two classes of WBCs (monocytes and lymphocytes) using a digital holographic microscope and a microfluidic system, and quantify the phase maps to enhance differentiation among different sizes of WBCs.
Review
Optics
Marika Valentino, Daniele Gaetano Sirico, Pasquale Memmolo, Lisa Miccio, Vittorio Bianco, Pietro Ferraro
Summary: Microplastic pollution poses a serious threat to the global environment, prompting the development of new identification and characterization methods. Digital holography (DH) has emerged as a high-throughput tool for detecting microplastics in flowing systems. This review discusses advances in microplastic screening using DH, examining the hardware and software aspects. The role of artificial intelligence in classification and regression tasks for automatic analysis based on smart DH processing is highlighted. Furthermore, the continuous development and availability of field-portable holographic flow cytometers for water monitoring are also discussed.
Article
Biochemistry & Molecular Biology
Gabriela Betlej, Dominika Bloniarz, Anna Lewinska, Maciej Wnuk
Summary: The use of non-targeting control siRNA (NTC-siRNA) can induce apoptosis and cell death in skin cancer cells, but not in fibroblasts. This suggests that NTC-siRNA may have unexpected effects in genetic engineering.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Review
Biochemistry & Molecular Biology
Leila Noori, Kamila Filip, Zohreh Nazmara, Simin Mahakizadeh, Gholamreza Hassanzadeh, Celeste Caruso Bavisotto, Fabio Bucchieri, Antonella Marino Gammazza, Francesco Cappello, Maciej Wnuk, Federica Scalia
Summary: Many neurodegenerative disorders are caused by the abnormal aggregation of misfolded proteins, which can self-replicate, transmit between cells, and induce the same protein in nearby cells. The distribution and toxicity of these accumulated proteins are involved in the progression of nervous system degeneration. Molecular chaperones help maintain protein stability and eliminate misfolded proteins, but their efficiency declines with age, leading to the development of neurological disorders. Extracellular vesicles (EVs) play a significant role in cellular communication and can transport disease-causing entities, contributing to the spread of damage in neurodegeneration. However, EVs also show potential in delivering therapeutic factors to the nervous system and can cross the blood-brain barrier (BBB). This review discusses the inefficiency of chaperone systems and the role of EVs in neurodegeneration, their potential as diagnostic biomarkers, and a promising EV-based therapeutic approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Daniele Pirone, Lu Xin, Vittorio Bianco, Lisa Miccio, Wen Xiao, Leiping Che, Xiaoping Li, Pasquale Memmolo, Feng Pan, Pietro Ferraro
Summary: Digital holography enables access to the full 3D information of flowing cells, leading to improved cell identification and potential in recognizing drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Physical
Mattia Zangoli, Andrea Cantelli, Andrea Candini, Anna Lewinska, Federica Fardella, Angela Tino, Giuseppina Tommasini, Maciej Wnuk, Matteo Moschetta, Sara Perotto, Marco Lucarini, Claudia Tortiglione, Guglielmo Lanzani, Francesca Di Maria
Summary: Here we demonstrate that thiophene-based core@shell nanoparticles, P3HT@PTDO NPs, exhibit a unique photoreactivity due to nanosegregation. The nanosegregation of the materials leads to the promotion of photogenerated electrons from the inner core to the outer shell, making the nanoparticles more reactive to the environment. Fluorometric and EPR techniques confirm the photoredox reactivity of the nanoparticles, with the formation of transient ROS. The biocompatibility of these nanoparticles is also demonstrated in cells and Hydra polyps.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Physiology
Marika Valentino, Vittorio Bianco, Lisa Miccio, Pasquale Memmolo, Valentina Brancato, Paolo Libretti, Marcello Gambacorta, Marco Salvatore, Pietro Ferraro
Summary: Kidney microscopy is essential for studying the morphology, physiology, and pathology of kidney tissues, providing important results for diagnosis. Fourier Ptychography (FP) is a microscopy technique that offers high-resolution images and a wide field of view, making it an attractive option for histopathology. This study presents the first comprehensive collection of kidney tissue images captured by an FP microscope, demonstrating the potential of FP microscopy in clinical practice for kidney histopathology.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Giuseppina Tommasini, Mariarosaria De Simone, Silvia Santillo, Gwennael Dufil, Marika Iencharelli, Daniele Mantione, Eleni Stavrinidou, Angela Tino, Claudia Tortiglione
Summary: This study investigates the neuromodulatory effects of an organic oligomer on the small invertebrate Hydra. The results show that the oligomer induces precise behavioral responses in Hydra and enhances muscle contraction frequency by affecting electrical activity. This research opens up new possibilities for studying organic bioelectronic interfaces and their clinical applications.
Article
Optics
Daniele Pirone, Daniele Sirico, Lisa Miccio, Vittorio Bianco, Martina Mugnano, Danila del Giudice, Gianandrea Pasquinelli, Sabrina Valente, Silvia Lemma, Luisa Iommarini, Ivana Kurelac, Pasquale Memmolo, Pietro Ferraro
Summary: The latest discoveries in the biochemical field emphasize the increasing importance of lipid droplets (LDs) in cellular regulatory mechanisms. Traditional methods like fluorescence microscopy and transmission electron microscopy only provide 2D images and partial measurements, limiting analysis of large cell numbers. We demonstrate for the first time the capability of 3D visualization and full LD characterization using a tomographic phase-contrast flow-cytometer, providing more in-depth understanding and new discoveries on the correlation between LDs and cellular functions.
OPTO-ELECTRONIC ADVANCES
(2023)
Article
Chemistry, Multidisciplinary
Volodymyr Tkachenko, Sara Coppola, Veronica Vespini, Daniele Tammaro, Pier Luca Maffettone, Pietro Ferraro, Simonetta Grilli
Summary: Studied the dynamics of oscillation and jetting of high viscosity liquid dielectrics in an electric field, and explored the viscosity dependence of the fluid. This study helps in the appropriate design of EHD processes and offers possibilities for rheological characterization of liquid polymers at nanoliter scale.
Article
Oncology
Pawel Przybylski, Anna Lewinska, Iwona Rzeszutek, Dominika Bloniarz, Aleksandra Moskal, Gabriela Betlej, Anna Deregowska, Martyna Cybularczyk-Cecotka, Tomasz Szmatola, Grzegorz Litwinienko, Maciej Wnuk
Summary: In this study, three mitochondria-targeted quercetin derivatives were synthesized and tested against six breast cancer cell lines with different mutation and receptor status. The activity of the quercetin derivatives was found to be modulated by the mutation status. One derivative induced apoptosis in breast cancer cells at a concentration of 1 μM. The derivatives also eliminated doxorubicin-induced senescent breast cancer cells and sensitized these cells to apoptosis.
Article
Optics
Jaromir Behal, Pasquale Memmolo, Lisa Miccio, Vittorio Bianco, Pietro Ferraro
Summary: Digital holography technology allows encoding complex information onto digital supports, with potential applications in automotive, video surveillance, and homeland security. Self-interference digital holography (SIDH) is investigated as a method for obtaining refocusable wavefronts using low-coherence light sources. A model is developed and analyzed to study the optical performance of SIDH, and a configuration for enhancing resolution is proposed. Experimental verification is conducted in the visible range, and a proof of concept SIDH imaging in the far infrared range is provided.
OPTICS AND LASER TECHNOLOGY
(2024)
Article
Automation & Control Systems
Anat Cohen, Matan Dudaie, Itay Barnea, Francesca Borrelli, Jaromir Behal, Lisa Miccio, Pasquale Memmolo, Vittorio Bianco, Pietro Ferraro, Natan T. Shaked
Summary: A new label-free imaging flow cytometry method is proposed for noninvasive and automated biological cell classification. By acquiring off-axis holograms of each cell from multiple viewpoints and using a modified ResNet-18 deep convolutional neural network, highly discriminating features are extracted for cell detection, classification, and differentiation. The method shows improved accuracy in classifying different types of cells and reduces the number of cell examples needed for training.
ADVANCED INTELLIGENT SYSTEMS
(2023)