Article
Biophysics
Shulian Bai, Bangtian Xu, Jiangling Wu, Guoming Xie
Summary: The study introduces the mechanisms of series and parallel toehold-mediated strand displacement for efficient chemical reactions when the target area is obstructed by secondary structures. By adjusting the length of the toeholds, reaction rates can be regulated and multifunctional regulation and selectivity can be achieved. The impressive function of this approach is demonstrated in a logic operation system.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Erica Del Grosso, Patrick Irmisch, Serena Gentile, Leonard J. Prins, Ralf Seidel, Francesco Ricci
Summary: This study presents a general approach to achieve dissipative control over toehold-mediated strand-displacement, a widely used reaction in DNA nanotechnology. By re-engineering the classic reaction, the high-energy invader strand is converted into a low-energy waste product, allowing the system to spontaneously return to its original state over time. This method enables unique temporal activation of DNA systems and is reversible and highly controllable.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Jinbo Zhu, Filip Boskovic, Bao-Nguyen T. Nguyen, Jonathan R. Nitschke, Ulrich F. Keyser
Summary: Introducing an external stimulus (Fe4L4)-L-II tetrahedral cage enables modulation of SDR kinetics. The binding of the cage on the toehold end slows down SDR, while stabilizing intermediates containing two overhangs accelerates the reaction.
Article
Biophysics
Shuting Li, Longjiao Zhu, Shenghao Lin, Wentao Xu
Summary: Toehold-mediated strand displacement (TMSD) reactions provide a fast, programmable and efficient way for DNA rehybridization. TMSD reactions have been widely used in biochemical sensing and medical diagnosis due to their enzyme-free and isothermal nature. The field faces challenges and has promising prospects.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Shuang Li, Disong Zhao, Fangfang Yang, Shufeng Liu
Summary: A general strategy for programming self-resettable and dissipative toehold-mediated strand displacement reactions was proposed using DNA strands as fuel and lambda exonuclease as the fuel-consuming unit. This strategy enables a reversible and temporally controllable non-equilibrium system that can be integrated into a DNA network to temporally control cascade reactions or dynamic behavior.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Zhang Zhang, Zhi Weng, Juan Yao, Dan Liu, Li Zhang, Limei Zhang, Guoming Xie
Summary: Small variations in nucleic acid sequences can have significant impact on diseases. We developed a PCR-based method to construct a high-selectivity long stranded detection probe, which was successfully used for genotyping G6PD deficiency in humans. This method has potential applications in disease diagnosis and treatment selection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Minghao Hu, Ziyi Chu, Haitao Wang, Wenbo Zhao, Tongbo Wu
Summary: Strand replacement technology faces various and complex applications as a component in the DNA machine and DNA network. By modifying remote toehold-mediated strand displacement, we have enabled it to operate with asymmetric spacer and asymmetric responses to spacer hybridization. Moreover, we have discovered a series of unique modulations, which will expand the application scope of strand displacement technology.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Wenchan Deng, Jing Yang Xu, Hanyong Peng, Cheng Zhi Huang, X. Chris Le, Hongquan Zhang
Summary: This study reports a toehold-exchange translator and translator-mediated DNAzyme motor systems that enable sensitive responses to various nucleic acid targets using the same DNAzyme motor without requiring redesign. The translator efficiently converts nucleic acid targets into specific output DNA, activating the DNAzyme motor and enabling autonomous movement. By adjusting the target-binding region of the translator, the same DNAzyme motor system can respond to multiple nucleic acid targets. The study also demonstrates the construction and operation of four logic gates using the translator and DNAzyme motor, enhancing the biosensing applications of DNA motors.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yeru Wang, Yu Wang, Su Liu, Wenyu Sun, Manru Zhang, Long Jiang, Minghan Li, Jinghua Yu, Jiadong Huang
Summary: A smart tripedal DNA walker is designed in this research, which can move freely on the electrode with improved walking efficiency, eliminating the drawbacks of high cost and instability, showing potential in OTA detection applications.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Dan Bai, Xi Zhou, Wang Luo, Hongyan Yu, Shulian Bai, You Wu, Lin Song, Kena Chen, Yaxing Xie, Xueping Chen, Jie Zhao, Yixin Fu, Yujun Yang, Junjie Li, Guoming Xie
Summary: Nucleic acid nanotechnologies based on toehold-mediated strand displacement have shown great potential for detecting single-nucleotide variations (SNVs). In this study, a cooperative hybridization strategy relying on a dual-toehold and bulge-loop (DT & BL) probe, coupled with the strand displacement catalytic (SDC) cycle, was developed to improve the specificity and reaction rate. The feasibility of this platform was demonstrated by successfully identifying cancer-related genes and detecting clinical samples. This study offers a new perspective for constructing highly specific probes based on dynamic DNA nanotechnology, and provides a promising tool for clinical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Yan Shan Ang, Lin-Yue Lanry Yung
Summary: The research team improved the performance of DNA circuits by dynamically elongating the length of the associative toehold using a hairpin lock design, and established design guidelines to achieve robust elongation without incurring additional leakages. Three hairpin initiators with different combinations of elongated associative toehold were shortlisted from the design framework for further discussion, resulting in improved reaction kinetics, equilibrium signal generation, and limit of detection. Overall, the elongated associative toehold acted as a built-in function to stabilize and favor the forward, desired reaction when triggered.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Analytical
Shu Zhang, Jiaoyan Yan, Ye Yang, Fei Mo, Yan Li, Hui Huang, Lichao Fang, Jian Huang, Junsong Zheng
Summary: This study developed a facile, rapid, and highly sensitive electrochemical biosensor for the detection and site analysis of DNA methylation. The method showed high sensitivity and rapid response, and did not require complex processing steps. It has the potential to be valuable for early clinical diagnosis and risk assessment.
Article
Chemistry, Analytical
Zhang Zhang, Li Zhang, Youqiang Wang, Juan Yao, Ting Wang, Zhi Weng, Liu Yang, Guoming Xie
Summary: This study introduces an ultrasensitive electrochemical miRNA biosensor based on strand displacement reaction circuits and strand displacement polymerization reaction, demonstrating high sensitivity and discriminatory capability towards let 7a for early cancer screening, providing a new research avenue.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Qiufeng Song, Chan Li, Yinghao Cheng, Linwen Lan, Ruize Cheng, Jing Zhang, Guoqiao Huang, Zhifa Shen, Chang Xue
Summary: DNA strand displacement reaction is important in dynamic DNA nanotechnology, but its poor specificity and kinetics limit its practical applications. A Multivalent dual lock-and-key (Multi-DLK) system is introduced here for accurate activation and highly specific nucleotide polymorphism assay. This system utilizes toehold-mediated strand displacement reactions to perform a toehold-mediated orthogonal DNA circuitry (ODC) and a highly target-specific strand displacement amplification (TSSDA) for gene typing. Comparative analysis and real sample applications demonstrate the potential of the Multi-DLK system for accurate and timely diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Jialin Zhang, Wenjing Liu, Jinhui Li, Kangqiang Lu, Herui Wen, Jiali Ren
Summary: A new electrochemical platform using a 3D walking machine and enzyme-free toehold-mediated strand displacement was developed for foodborne pathogen detection. The method showed low detection limits and short detection time, making it promising for early diagnosis.
Article
Chemistry, Analytical
Yifan Jiang, Xin Xu, Xiao Fang, Shuxian Cai, Min Wang, Chao Xing, Chunhua Lu, Huanghao Yang
ANALYTICAL CHEMISTRY
(2020)
Article
Nanoscience & Nanotechnology
Tao Guo, Qiuyu Tang, Yating Guo, Honglong Qiu, Jing Dai, Chao Xing, Shihao Zhuang, Guoming Huang
Summary: Photothermal therapy is a promising new tumor treatment method, and the study found that the synthesized boron quantum dots have excellent biological safety and high photothermal conversion ability, making them suitable for tumor photothermal therapy applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Qitian Lin, Junye Wu, Lili Jiang, Dexian Kong, Chao Xing, Chunhua Lu
Summary: The study developed an electrochemical biosensor based on Mg2+-dependent DNAzyme for simultaneous detection of miRNA-21 and miRNA-141, showing high sensitivity and selectivity. The biosensor offers advantages such as simple preparation and enzyme-free signal amplification, making it suitable for various bioanalysis and clinical diagnostics.
Article
Chemistry, Multidisciplinary
Wukun Zhong, Junye Wu, Yuqing Huang, Chao Xing, Chunhua Lu
Summary: By utilizing a target-activated, light-actuated three-dimensional DNA walker, this study presents a novel method for sensitive detection of miRNA on gold nanoparticles. The technique offers simplicity, sensitivity, and high specificity, and can be applied to serum samples.
Article
Chemistry, Multidisciplinary
Chao Xing, Shan Chen, Qitian Lin, Yuhong Lin, Min Wang, Jun Wang, Chunhua Lu
Summary: A DNA origami amplifier was designed for the accurate detection of low-abundance miRNA in living cells, showing high sensitivity and stability for potential application in early-stage clinical diagnosis.
Article
Nanoscience & Nanotechnology
Chao Xing, Qitian Lin, Xue Gao, Ting Cao, Jing Chen, Jialing Liu, Yuhong Lin, Jun Wang, Chunhua Lu
Summary: The study demonstrates a self-powered and self-feedback DNAzyme amplification circuit using MnO2 nanosheet-mediated entropy-driven catalyst (EDC)-DNAzyme nanoprobe for sensitive imaging of intracellular microRNA. This strategy provides a versatile approach for the construction of self-powered and self-feedback DNA circuits, enabling effective detection of intracellular molecules with enhanced sensitivity.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Tao Zeng, Jiahui Fang, Yifan Jiang, Chao Xing, Chunhua Lu, Huanghao Yang
Summary: This study reports a new type of spherical nucleic acid probe based on 2'-fluorinated DNA-functionalized gold nanoparticles, which exhibit excellent stability in high salt conditions and a broad pH range, as well as resistance to biothiols. The probe shows high accuracy in detecting targeted cancer cells and can discriminate target cancer cells in a mixed culture system.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Ziyi Chen, Xin Wang, Yihang Ding, Chao Xing, Chunhua Lu, Xiankun Tu
Summary: Chemodynamic therapy (CDT) is a catalytic anticancer strategy that utilizes transition metal ions to produce cytotoxic hydroxyl radicals (center dot OH) in situ. However, existing CDTs are limited by the presence of antioxidant molecules and enzymes in the tumor environment, which can suppress CDT. To overcome this limitation, self-assembled copper-selenocysteine nanoparticles (Cu-SeC NPs) were developed to enhance center dot OH-induced cellular damage and depletion of antioxidants. The Cu-SeC NPs exhibit synergistic antitumor effects and negligible systemic toxicity, providing a new avenue for therapeutic development.
ACS MATERIALS LETTERS
(2023)
Article
Materials Science, Multidisciplinary
Ziyi Chen, Xin Wang, Yihang Ding, Chao Xing, Chunhua Lu, Xiankun Tu
Summary: Chemodynamic therapy (CDT) utilizes transition metal ions to produce cytotoxic hydroxyl radicals (center dot OH) for anticancer treatment. However, current CDTs are limited by the presence of antioxidant molecules and enzymes in tumors, which can deplete center dot OH. In this study, copper-selenocysteine nanoparticles (Cu-SeC NPs) were designed to enhance center dot OH-induced cellular damage by depleting antioxidants and inhibiting enzyme activity. The Cu-SeC NPs showed synergistic antitumor effects without systemic toxicity, providing a new avenue for therapeutic systems.
ACS MATERIALS LETTERS
(2023)
Article
Materials Science, Biomaterials
Kailong Zhang, Huanru Fu, Chao Xing, Yi Luo, Fangfang Cheng, Qiang Fu, Yujie Huang, Longxin Qiu
Summary: The study focused on inducing TNBC cell apoptosis to secrete ABAs for therapeutic drug delivery, which showed promising results in inhibiting TNBC growth and metastasis. ABAs possess desirable characteristics for targeted drug delivery therapy, particularly for efficient TNBC treatment. The results suggest the potential of ABAs in enhancing targeted drug delivery strategies.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
Chao Xing, Ziyi Chen, Yuhong Lin, Min Wang, Xin Xu, Junduan Dai, Jun Wang, Chunhua Lu
Summary: In this study, an accelerated DNA tetrahedron based molecular beacon was developed for efficient detection and imaging of miRNA in living cells. The research provides an improved strategy for fluorescence amplified RNA imaging in early clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Nanoscience & Nanotechnology
Chao Xing, Ziyi Chen, Cheng Zhang, Jun Wang, Chunhua Lu
ACS APPLIED BIO MATERIALS
(2020)
Article
Materials Science, Biomaterials
Chao Xing, Ziyi Chen, Cheng Zhang, Jun Wang, Chunhua Lu
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
Article
Chemistry, Analytical
Yuhong Lin, Yuqing Huang, Yuling Yang, Lili Jiang, Chao Xing, Jingying Li, Chunhua Lu, Huanghao Yang
ANALYTICAL CHEMISTRY
(2020)