Article
Materials Science, Biomaterials
Ridge Chavalala, Philani Mashazi
Summary: This study reports the fabrication of palladium nanoparticles decorated indium tin oxide-silica nanoparticles (ITO-SiO2-prS-PdNPs), which exhibit peroxidase-like activity and can be used for glucose detection. The nanozyme has advantages such as reusability, high selectivity, and excellent detection results.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Physical
Lili Xu, Jinxing Chen, Qian Ma, Daiyong Chao, Xinyang Zhu, Ling Liu, Jin Wang, Youxing Fang, Shaojun Dong
Summary: Polymer stabilizers greatly influence the structure, size, and activity of gold nanoparticles (Au NPs). Among the polymers tested, polyacrylic acid-stabilized Au NPs exhibit the highest glucose oxidase-like activity due to their small size and weak spatial resistance. However, further tests reveal limitations in selectivity and activity in physiological environments.
Review
Chemistry, Multidisciplinary
Mohamad Zandieh, Juewen Liu
Summary: The article discusses the issue of calculating catalytic turnover rates of nanozymes, emphasizing that only surface atoms should be considered as active sites. Different types of nanozymes exhibit varying catalytic activity characteristics, which may be influenced by the adsorption of reaction products.
Article
Chemistry, Inorganic & Nuclear
Zhencheng Sun, Jiajian Yao, Jidong Wang, Ruijia Huang, Xiaolei Liu, Feng Li, Xingyu Jiang, Wenwen Chen
Summary: A one-step approach is presented in this study to produce gold nanoparticle-bovine serum albumin nanocomposite (BSA-Au) with distinct oxidase-like activity and stability, which can be used in water sterilization and antibacterial applications. This strategy overcomes the limitations of strict synthesis conditions and precise size/structure control, allowing for bulk production of nanozymes for broad application.
INORGANIC CHEMISTRY
(2022)
Article
Biochemical Research Methods
Xiaoting Yang, Chenxi Feng, Aizhu Peng, Qian Wang, Zhong-Yi Liu, Feng Pei, Jianshuai Mu, En-Cui Yang
Summary: In this work, bimetallic FeMn@C nanozyme with efficient peroxidase-like activity was synthesized and studied. The results showed that FeMn@C nanozyme exhibited 16 times higher peroxidase-like activity than single-metal Fe@C. FeMn@C nanozyme had better affinity for substrates and stability compared to natural enzymes. Furthermore, a colorimetric assay for glucose detection based on FeMn@C nanozyme was constructed, showing good selectivity and accuracy.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xuan-Hung Pham, Van-Khue Tran, Eunil Hahm, Yoon-Hee Kim, Jaehi Kim, Wooyeon Kim, Bong-Hyun Jun
Summary: This study reports a novel method for preparing bimetallic nanoparticles with high activity using seed-mediated growth on a silica core template. The resulting nanoparticles exhibit excellent peroxidase-like nanozyme activity and high sensitivity for H2O2 detection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Xiulian Yin, Peng Liu, Xuechao Xu, Jianming Pan, Xin Li, Xiangheng Niu
Summary: Research on nanomaterials mimicking enzymes has been increasing, with particular interest in materials mimicking peroxidase due to their potential applications. However, most currently explored peroxidase nanozymes exhibit favorable activity only in acidic environments, presenting a challenge to develop mimics with good catalytic performance across a broad pH range.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Shivendra Singh, Tushar Kanti Mukherjee
Summary: This study presents a facile and robust methodology for the in situ synthesis of gold nanoparticles (Au NPs) inside quantum dot-embedded coacervate droplets (QD-Ds) as confined compartments. The synthesized Au NPs exhibit superior peroxidase-like activity and excellent recyclability over several consecutive cycles without any compromise. These hybrid plexcitonic assemblies have potential applications in bioanalytical chemistry and optoelectronics.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Qiulan Li, Hong Li, Kexiang Li, Yi Gu, Yijie Wang, Dezhi Yang, Yaling Yang, Lei Gao
Summary: A novel colorimetric nanoprobe for the rapid and specific detection of methylmercury is proposed based on the selective enhancement of nanozyme activity. The formation of gold amalgamation between nanozyme and methylmercury allows for the accelerated generation of radicals and peroxidase-like activity. This method provides a new perspective for the analysis of organometallic compounds.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemical Research Methods
Sanjana Naveen Prasad, Pabudi Weerathunge, Md. Nurul Karim, Samuel Anderson, Sabeen Hashmi, Pyria D. Mariathomas, Vipul Bansal, Rajesh Ramanathan
Summary: This study introduces a free-standing copper NanoZyme for colorimetric detection of glucose in human urine, demonstrating high robustness and biological relevance.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Javier Bonet-Aleta, Jose I. Garcia-Peiro, Silvia Irusta, Jose L. Hueso
Summary: Nanozymes are nanomaterials that mimic the catalytic activity of natural enzymes and are widely used in the development of biosensing analytical tools. In this study, a gold-platinum nanoparticle with core-shell configuration was synthesized and exhibited remarkable oxidase-like activity towards specific substrates. By exploiting the loss of activity, an analytical method was developed to detect and quantify glutathione levels.
Article
Biophysics
Ruolan Zhou, Xiaohong Zhuang, Qiaoli Wu, Ming Jin, Chaochuan Zheng, Yayun Jiang, Yongliang Lou, Laibao Zheng
Summary: In this study, an artificial enzyme Cu-MOF@Pt based on nanomaterials was successfully prepared, which demonstrated its selective detection ability for glucose. This method has broad application prospects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Ridge Chavalala, Philani Mashazi
Summary: Nanomaterials of gold and palladium were fabricated as thin nanoparticle films on catalytically inert surfaces, and their recovery and reuse were investigated. The bimetallic nanomaterial film demonstrated excellent peroxidase-like activity and was used for the detection of ascorbic acid.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Chihiro Mochizuki, Junna Nakamura, Michihiro Nakamura
Summary: This study investigates the efficiency of gold nanoparticles (Au NPs) embedded in thiol-organosilica (thiol-OS/Au series) as radiosensitizers for enhancing cell damage in radiotherapy. The results show that thiol-OS/Au embedded with small Au NPs exhibited peroxidase-like activity under acidic conditions, improving reactive oxygen species generation and cytotoxicity under X-ray irradiation. Additionally, this sample exhibited catalase-like activity under basic conditions and showed no cytotoxicity toward nonirradiated cells. These findings suggest the potential of functionalizing small Au NPs with pH-controlled enzyme-like activities as radiosensitizers.
Review
Biochemical Research Methods
Ruiying Zhao, Haile Liu, Yongming Li, Meili Guo, Xiao-Dong Zhang
Summary: Radiotherapy is commonly used in cancer treatment, but it can cause damage to surrounding normal tissues. Nanozymes show potential as radioprotective agents, offering a high-efficiency solution for radiation protection.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Omotayo Adeniyi, Simbongile Sicwetsha, Abiola Adesina, Philani Mashazi
Summary: A colorimetric immunosensor was developed for the detection of tumor-associated anti-p53 autoantibodies using a platform based on nanomagnet-silica nanoparticles and a high binding 96-well plate. The immunosensor showed high binding affinity and catalytic performance, leading to successful detection of anti-p53aAbs with a lower detection limit compared to conventional methods. The real sample detection using the serum recovery method demonstrated the potential for practical applications with a recovery rate of 98.5% to 105.7%.
Article
Chemistry, Analytical
Omotayo Adeniyi, Nnamdi Nwahara, Daniel Mwanza, Tebello Nyokong, Philani Mashazi
Summary: The current diabetes management systems rely on invasive blood sampling methods, but a non-invasive and painless saliva glucose measurement offers a desirable alternative for constant monitoring. The GCE-SWCNT/rGO/CoPc sensor, with its high sensitivity, stability, and selectivity, shows great potential for accurate and non-invasive glucose sensing in saliva. The sensor exhibits excellent sensitivity, specificity, storage stability, reusability, and fast response time for glucose detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Abiola Adesina, Omotayo Adeniyi, Philani Mashazi
Summary: Selective purification of biological materials for their detection in complex sample matrix is a general challenge in diagnostics. In this study, an ultrasensitive sandwich-based impedimetric immunosensor was developed for the detection of CRP antigen. The stable and oriented immobilization of anti-CRP monoclonal antibody on magnetic nanoparticles was achieved, and Electrochemical Impedance Spectroscopy was used for CRP detection.
Article
Electrochemistry
Iphithuli Moyo, Daniel Mwanza, Philani Mashazi
Summary: In this study, a gold electrode surface was pre-grafted with a phenylethylamine (PEA) thin film, allowing for the spontaneous covalent immobilization of cobalt (II) octa acyl chloride (CoOAClPc). The pre-grafting of the PEA thin film was achieved through the electrochemical reduction of 4-(2-aminoethyl) benzene diazonium (AEBD) salt. The resulting Au-PEA-CoOCAPc thin film exhibited pH sensitivity and excellent analytical properties for the detection of catecholamine neurotransmitters.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Omotayo Adeniyi, Philani Mashazi
Summary: A paper-based immunodevice integrated with a smartphone platform has been developed for ultrasensitive and rapid detection of anti-p53 autoantibodies in human serum samples. The immunodevice, fabricated using kirigami principles, showed a linear increase in color pixel intensity with increasing concentrations of anti-p53 autoantibodies, demonstrating its potential for cancer diagnosis at point-of-care.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Daniel Mwanza, Nololo Mfamela, Omotayo Adeniyi, Tebello Nyokong, Philani Mashazi
Summary: This study achieved the preparation of glucose encapsulating nanoliposomes using two different lipid formulations. The F2 formulation showed the highest controlled release at acidic pH and was used for the indirect detection of PSA. The PGM system had the lowest limit of detection due to its sensitivity to glucose. The Pd | PdO nanozyme system demonstrated good stability at high temperatures.
Article
Chemistry, Multidisciplinary
Songeziwe Ntsimango, Sendibitiyosi Gandidzanwa, Sinelizwi V. Joseph, Eric C. Hosten, Marvin Randall, Adrienne L. Edkins, Samson M. Khene, Philani Mashazi, Tebello Nyokong, Abubak'r Abrahams, Zenixole R. Tshentu
Summary: A novel method of accessing rhenium(V)-phthalocyanine complexes through metalation and subsequent nanoparticle synthesis has potential for tumor targeting applications.
Article
Chemistry, Physical
Ene Storm, Emile D. Maggott, Philani Mashazi, Tebello Nyokong, Rehana Malgas-Enus, Selwyn F. Mapolie
Summary: Gold and palladium nanoparticles were decorated on melamine formaldehyde and melamine resorcinol formaldehyde microspheres as heterogeneous catalysts for the oxidation of 1-phenylethanol. The supported catalysts showed higher conversion rates (around 83%) in water solvent compared to acetonitrile solvent (48-50%), which can be attributed to the different stability of the oxidant in different solvents.
MOLECULAR CATALYSIS
(2022)
Article
Chemistry, Analytical
Abiola Adesina, Omotayo Adeniyi, Philani Mashazi
Summary: Cardiovascular diseases (CVDs), which are diseases that affect the heart and blood vessels, are often fatal when diagnosis is delayed. This study focuses on the development of an immunosensor for the detection and monitoring of C-reactive protein (CRP), a biomarker for cardiac events. By controlling the orientation of the antibodies, false positive and negative results during sample analysis can be eliminated. The developed detection protocol showed excellent selectivity and specificity towards CRP, with the ability to regenerate the electrode for repeated measurements.
ANALYTICAL METHODS
(2023)
Article
Nanoscience & Nanotechnology
Ridge Chavalala, Philani Mashazi
Summary: Nanomaterials of gold and palladium were fabricated as thin nanoparticle films on catalytically inert surfaces, and their recovery and reuse were investigated. The bimetallic nanomaterial film demonstrated excellent peroxidase-like activity and was used for the detection of ascorbic acid.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
N. Nwahara, M. Motaung, G. Abrahams, P. Mashazi, J. Mack, E. Prinsloo, T. Nyokong
Summary: This study reports on the design and synthesis of a novel photosensitizer complex that can release singlet oxygen and nitric oxide, demonstrating potential for use in photodynamic therapy by causing lethal phototoxic effects on cancer cells.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Electrochemistry
Kayode Omotayo Adeniyi, Blerina Osmanaj, Gopinathan Manavalan, Ajaikumar Samikannu, Jyri-Pekka Mikkola, Berisha Avni, Jean-Francois Boily, Solomon Tesfalidet
Summary: Nanostructure kazakhstanite-like iron vanadate (FexV3xOy.H2O) was synthesized and calcined at different temperatures to modify screen-printed carbon electrodes for electrocatalytic effect. The effects of calcination conditions on the catalytic performance of the electrode towards chemotherapeutic drugs were studied. The modified electrode exhibited high sensitivity and selectivity for the detection of methotrexate and folinic acid in blood serum.
ELECTROCHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Sendibitiyosi Gandidzanwa, Natasha Beukes, Sinelizwi Joseph, Arno Janse Van Vuuren, Philani Mashazi, Jonathan Britton, Gareth Kilian, Saartjie Roux, Tebello Nyokong, Michael E. Lee, Carminita L. Frost, Zenixole R. Tshentu
Summary: Folate receptor-targeted therapy shows promise in breast cancer treatment. Folate-conjugated palladium-based nanoparticles with non-toxic concentrations were utilized to specifically target breast cancer cells with overexpressed folate receptors. The nanoparticles were found to selectively accumulate in cancer cells relative to normal cells, as demonstrated by qualitative and quantitative analysis using transmission electron microscopy and inductively coupled plasma-optical emission spectroscopy. These findings support the potential use of folate-conjugated palladium nanoparticles in targeting breast cancer cells for radiopharmaceutical applications.
Article
Materials Science, Biomaterials
Ridge Chavalala, Philani Mashazi
Summary: This study reports the fabrication of palladium nanoparticles decorated indium tin oxide-silica nanoparticles (ITO-SiO2-prS-PdNPs), which exhibit peroxidase-like activity and can be used for glucose detection. The nanozyme has advantages such as reusability, high selectivity, and excellent detection results.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
