Article
Chemistry, Analytical
Ji Zheng, Dayong Li, Jin Jiao, Chengjie Duan, Zhongyun Wang, Yang Xiang
Summary: In this study, a dual-aptamer recognition strategy based on G-quadruplex nanowires was developed for selective analysis of exosomes, allowing sensitive and specific analysis of target exosomes. This method is simple and universal for different types of exosomes, showing great significance for clinical cancer diagnosis.
Article
Biochemistry & Molecular Biology
David Moreira, Daniela Leitao, Jessica Lopes-Nunes, Tiago Santos, Joana Figueiredo, Andre Miranda, Daniela Alexandre, Candida Tomaz, Jean-Louis Mergny, Carla Cruz
Summary: In this study, the formation and stability of G-quadruplex structure of a G-rich DNA aptamer AT11-L2 were explored under different experimental conditions, along with its interactions with various G4 ligands and nucleolin (NCL). The results showed that AT11-L2 adopted different topologies depending on the KCl concentration, and the addition of ligands significantly stabilized the G-quadruplex structure. Furthermore, a specific interaction between AT11-L2 G-quadruplex and NCL was identified, and co-localization of these two molecules was observed in certain cell lines.
Review
Chemistry, Physical
Jerome Dejeu, Angeline Van der Heyden, Nicolas Spinelli, Eric Defrancq, Liliane Coche-Guerente
Summary: Aptamer-based electrochemical sensors have been developed for detecting a wide variety of analytes with high selectivity, sensitivity, and stability, using specific G-quadruplex (G4) conformations for molecular recognition. These sensors can be miniaturized, have low production costs, and offer great flexibility in design assemblies.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Weronika Kotkowiak, Anna Pasternak
Summary: G-quadruplexes play a crucial role in nucleic acid structures and can be utilized as regulatory elements in cells. Extending quadruplexes with additional structural elements enhances stability and biological activity, and the specific interaction of G-quadruplexes with target molecules is pivotal. Future applications of G-quadruplex junctions hold promise, and there is still room for development in emerging research areas.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Masataka Mizunuma, Mirai Suzuki, Tamaki Kobayashi, Yuki Hara, Atsushi Kaneko, Kazuhiro Furukawa, Yoshiro Chuman
Summary: The development of new molecules for monitoring metal ion concentrations with high sensitivity and selectivity is important. DNA, especially guanine-rich DNA, can be used in metal ion-detection sensors and functional materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Rana Bagheri, Fathallah Karimzadeh, Ahmad Kermanpur, Mahshid Kharaziha
Summary: A simple and novel method for immobilization of AS1411 aptamer on Cu nanoparticles electrode was presented and validated by experiments. The study found that choosing 0.03 M Cu concentration, 10 μM aptamer concentration, and 20 minutes incubation time achieved the optimized performance, which was confirmed by quartz crystal microbalance.
Article
Multidisciplinary Sciences
Long Li, Shujuan Xu, Xueyu Peng, Yuzhuo Ji, He Yan, Cheng Cui, Xiaowei Li, Xiaoshu Pan, Lu Yang, Liping Qiu, Jianhui Jiang, Weihong Tan
Summary: The use of aptamers in bio analytical and biomedical applications exploits their ability to recognize cell surface protein receptors, making them valuable tools in targeted therapeutics. A strategy of constructing structure-switching aptamers has been developed, enhancing target cell recognition by engineering aptamers with DNA intercalated motifs responsive to the microenvironment. Structure-switching sensitivity can be tuned by manipulating i-motif sequences, but estimating this sensitivity is challenging.
NATIONAL SCIENCE REVIEW
(2021)
Article
Chemistry, Multidisciplinary
Shenqi Han, Lin Xue, Yi Wei, Tuying Yong, Wenlong Jia, Yongqiang Qi, Yiming Luo, Junnan Liang, Jingyuan Wen, Nana Bie, Huifang Liang, Qiumeng Liu, Zeyang Ding, Xiangliang Yang, Lu Gan, Zhao Huang, Xiaoping Chen, Bixiang Zhang
Summary: Bone metastasis is a common occurrence in patients with hepatocellular carcinoma (HCC) and is associated with a poor prognosis. This study investigates the role of extracellular vesicles (EVs) in mediating the communication between primary HCC and bone lesions, and their impact on HCC progression. It is found that bone-metastasized HCC-derived EVs (BM-EVs) promote HCC progression by transferring a specific microRNA, miR-3190, to orthotopic tumor cells, which downregulates the expression of ALKBH5 and enhances their metastatic capacity. Inhibition of miR-3190 using antagomir-loaded liposomes effectively suppresses HCC progression in mice treated with BM-EVs. These findings suggest miR-3190 as a promising therapeutic target for inhibiting the progression of HCC in patients with bone metastasis.
Article
Biochemistry & Molecular Biology
Chengcong Zhu, Lisha Gong, Yang Yang
Summary: In this study, we developed an exponential amplification-based label-free method for detecting exosomes, which can detect exosomes at low concentrations and has potential clinical applications.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rongxin Zhang, Yajun Liu, Xingxing Zhang, Ke Xiao, Yue Hou, Hongde Liu, Xiao Sun
Summary: The study profiles and depicts RNA G-quadruplexes in the human transcriptome using DMS-seq and bioinformatics pipeline, revealing their significant association with immune pathways and COVID-19-related gene sets. The bioinformatics analysis also suggests potential regulatory functions of G-quadruplexes on miRNA targeting at the whole transcriptome level. Moreover, G-quadruplexes are found to be depleted in putative, but not real, PAS-strong poly(A) sites, weakening their likelihood of being actual cleaved sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Xin Zheng, Shunxiang Gao, Jihong Wu, Xiaobo Hu
Summary: A fluorescent aptasensor based on an assembled G-quadruplex and ThT was developed for the rapid, accurate, and convenient detection of VEGF165. The sensor showed a good linear detection range, detection limit, accuracy, reproducibility, and stability when applied to clinical serum samples for VEGF165 detection.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Yanping Wu, Yachen Shi, Sha Deng, Chengyong Wu, Ruijie Deng, Guiping He, Mi Zhou, Kai Zhong, Hong Gao
Summary: The study introduced a unique aptamer probe construction method for label-free and ratiometric detection of lead in tea, showing high specificity and accuracy. The probe design utilizes dual fluorescent signal outputs under single-wavelength excitation for Pb2+ recognition, outperforming single fluorescent reporter-based analyses.
Article
Biochemistry & Molecular Biology
Zhuohao Xie, Peng Li, Haonan Zhan, Liuyan Zhuang, Sijian Tao, Liehua Deng, Jiahao Huang
Summary: An allosterically modulated G-quadruplex-integrated aptamer is designed for efficient and accurate detection of melamine in dairy products. The method is label-free, turn-on, low-cost, and time-efficient, with high specificity and a low detection limit. This approach can be easily manipulated and provides ultra-sensitive detection, making it useful in the field of food safety, biomedical engineering, and clinical diagnosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nanoscience & Nanotechnology
Sohini Pal, Akshay Naik, Anjana Rao, Banani Chakraborty, Manoj M. Varma
Summary: We demonstrate the single-molecule sensing of G-quadruplex formation in target-bound aptamers using solid-state nanopores. By functionalizing the nanopores with DNA origami sheets containing the aptamer sequence, we provide a versatile configuration for nanopore-based single-molecule sensors. The DNA origami sheet also includes a pore that can modulate the translocation dynamics of the target molecule. Using this system, we successfully differentiate the formation of G-quadruplex in the well-studied thrombin aptamer.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yi Du, Qiwei Wang, Lili Shi, Tao Li
Summary: DNA-assembled multiaptamer systems offer a promising approach for targeted delivery of nucleic acid cargoes. In this study, a G-quadruplex-proximized aptamer system was engineered for efficient transmembrane delivery of siRNA, showing great potential for precise anticancer therapy.