Article
Materials Science, Biomaterials
Xuanwen Xu, Xinyu Li, Shuang Qiu, Yi Zhou, Lu Li, Xu Chen, Kai Zheng, Yan Xu
Summary: This study aimed to explore the appropriate concentration ratio of biofriendly transglutaminase-modified gelatin hydrogels for promoting periodontal alveolar bone regeneration. Through a series of characterization and cell experiments, it was found that all the hydrogels possessed multi-space network structures and demonstrated their biocompatibility. In vivo and in vitro osteogenic differentiation experiments also confirmed that the group 40-5 (transglutaminase-gelatin concentration ratio) possessed a favorable osteogenic potential.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Applied
Hongrui Chen, Di Wu, Wuchao Ma, Chao Wu, Jia Liu, Ming Du
Summary: In this study, strong fish gelatin hydrogels were successfully prepared by double crosslinking gelatin with transglutaminase (TGase) and kappa-carrageenan, resulting in significantly improved mechanical properties and thermal stability. The highest gel strength, compression fracture stress, and storage modulus were achieved when the concentration of TGase was 20 U/g gelatin. The thermal stability of the double crosslinked gelatin hydrogels was attributed to the presence of covalent crosslinks. The effect of TGase concentration on the physical properties and microstructure of the double crosslinked hydrogels was analyzed, and the differences between double crosslinked gelatin hydrogels and gelatin hydrogels single crosslinked by TGase or carrageenan were systematically compared.
Article
Polymer Science
Alina Gabriela Rusu, Loredana Elena Nita, Natalia Simionescu, Alina Ghilan, Aurica P. Chiriac, Liliana Mititelu-Tartau
Summary: Development of a versatile hydrogel based on enzymatically-crosslinked gelatin and nanogels loaded with amoxicillin (Amox) is reported, demonstrating self-healing performance and tunable physical properties. The nanogels formed through self-assembly interactions contribute to the self-healing capacity and drug distribution within the hydrogel network. The physicochemical properties and drug release behavior of the hydrogels were studied, showing rapid release of amoxicillin in acidic conditions, indicating favorable characteristics for wound healing. In vitro and in vivo biocompatibility assays showed the potential of the optimized scaffolds as wound dressings.
Article
Chemistry, Applied
Yi Zhang, Chen Li, Timothy Geary, Armando Jardim, Shudong He, Benjamin K. Simpson
Summary: This study utilized a new psychrophilic transglutaminase to catalyze the formation of protein and peptide hydrogels, resulting in significantly improved antioxidant bioactivity. Molecular docking studies were performed to understand the mechanism behind the enhanced bioactivity of cross-linked hydrogels formed by the psychrophilic transglutaminase.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Jun Yan, Siqi Li, Guipan Chen, Cuicui Ma, David Julian McClements, Xuebo Liu, Fuguo Liu
Summary: This study investigated the effects of heat-induced and enzyme-induced gelation on the formation and properties of composite hydrogels prepared using whey protein isolate (WPI) and gelatin. The results showed that by changing the pH and crosslinking methods, the structure and texture of the composite hydrogels could be controlled, which may have potential applications in various industries including food.
FOOD HYDROCOLLOIDS
(2023)
Review
Polymer Science
Karen Guillen-Carvajal, Benjamin Valdez-Salas, Ernesto Beltran-Partida, Jorge Salomon-Carlos, Nelson Cheng
Summary: Hydrogels are versatile biomaterials with three-dimensional, cross-linked, highly hydrated polymeric networks. They have a variety of biochemical and biophysical properties, allowing for diffusion of diverse molecules and control of cellular interactions. Hydrogels can be molded into different structures resembling surrounding tissues and bone defects. Their application in bone tissue engineering has garnered significant attention due to the beneficial bone improvement results achieved. Synthetic polymers have played a key role, but there is a trend towards using biological precursors like chitosan, collagen, and gelatin for their high biocompatibility, degradability, and mechanical control.
Article
Biochemistry & Molecular Biology
Han D. Nguyen, Xun Sun, Hiroki Yokota, Chien-Chi Lin
Summary: It has been found that certain bone cells can suppress cancer cell migration and invasion through direct cell-cell contact and cytokine secretion. A gelatin-based hydrogel system with independently tunable matrix stiffness and viscoelasticity has been developed to support 3D osteocyte culture, offering a versatile platform for investigating mechano-sensing in osteocytes and producing potent anti-tumor conditioned media (CM). Adjusting the composition of the hydrogels can modulate cell behavior and cytokine secretion, potentially impacting tumor growth and invasion.
Article
Nanoscience & Nanotechnology
Nuri Park, Jaeyun Kim
Summary: The study introduces a tendon-mimicking hydrogel with an anisotropic structure through stretching, solvent exchange, and crosslinking. Anisotropic hydrogels exhibit high strength and toughness, while braiding them into hierarchical structures further improves mechanical performance.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
Alan Avila-Ramirez, Kevin Catzim-Rios, Carlos Enrique Guerrero-Beltran, Erick Ramirez-Cedillo, Wendy Ortega-Lara
Summary: This study presents the preparation of a novel ink for bone and cartilage tissue restoration, focusing on the synthesis of bioceramic materials, integration into a biopolymeric base matrix, and characterization of functional composite materials for future manufacturing using techniques such as 3D printing.
Article
Pharmacology & Pharmacy
Aldo F. Corona-Escalera, Ernesto Tinajero-Diaz, Rudy A. Garcia-Reyes, Gabriel Luna-Barcenas, Ali Seyfoddin, Jose Daniel Padilla-de la Rosa, Marisela Gonzalez-Avila, Zaira Y. Garcia-Carvajal
Summary: In this study, Lactiplantibacillus plantarum spp.CM-CNRG TB98 was entrapped in a Gel-PVA hydrogel using microbial transglutaminase as a crosslinking agent. The Gel-PVA hydrogel showed high loading efficiency and protection for L. plantarum under gastric conditions. This hydrogel is suitable for probiotic oral administration.
Article
Engineering, Biomedical
Aylin Kara, Thomas Distler, Christian Polley, Dominik Schneidereit, Hermann Seitz, Oliver Friedrich, Funda Tihminlioglu, Aldo R. Boccaccini
Summary: This study demonstrates the feasibility of using decellularized bone particles as reinforcement agents in gelatin-based scaffolds for bone tissue engineering. The fabricated 3D scaffolds showed homogenous distribution of the reinforcement agents and exhibited enhanced mechanical properties and degradation rates. The scaffolds also demonstrated bioactivity and cytocompatibility, making them potential candidates for bone tissue engineering.
MATERIALS TODAY BIO
(2022)
Article
Polymer Science
Shouwei Zhao, Zhiwei Chen, Yaqi Dong, Wenhui Lu, Deyi Zhu
Summary: Mechanically enhanced gelatin-based composite hydrogels were developed using functionalized cellulose nanocrystals (CNCs) and microbial transglutaminase (mTG) as a binding agent, resulting in significantly improved mechanical properties and thermo-stability. The biocompatibility of the hydrogels was evaluated and showed no obvious inhibitory effect on cell proliferation, indicating great potential for biomedical applications.
Article
Pharmacology & Pharmacy
Eduardo Anitua, Mar Zalduendo, Maria Troya, Itsasne Erezuma, Izeia Lukin, Raquel Hernaez-Moya, Gorka Orive
Summary: The study investigated the potential of three different bioinks based on gelatin and alginate for regenerative medicine. The bioinks containing hydroxyapatite and PRGF showed the highest cell compatibility and promoted osteogenic differentiation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Engineering, Biomedical
Fenyan Miao, Tingting Liu, Xiumei Zhang, Xuefeng Wang, Yan Wei, Yinchun Hu, Xiaojie Lian, Liqin Zhao, Weiyi Chen, Di Huang
Summary: In this study, GelMA and Alg were used to prepare a hydrogel scaffold that mimics the structure and function of natural extracellular matrix, and the physical properties and osteoinductivity were enhanced by mineralization with hydroxyapatite (HA). The mineral content and compressive strength of the mineralized hydrogel were significantly improved, confirming the potential of the GelMA/Alg-HA hydrogel for bone regeneration.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Engineering, Chemical
Qiaoqiao Han, Juncai Leng, Tiantian Dong, Yanli Ma, Wei Zhao
Summary: A novel gelatin with high crosslinking activity (type-E bone gelatin) was developed using enzymatic catalysis. The study found that this type-E gelatin had higher levels of lysine and glutamine compared to traditional gelatins prepared using acid or alkali methods, making it suitable for crosslinking with microbial transglutaminase (MTGase). The crosslinking degree of type-E gelatin was significantly higher than that of type-A and type-B gelatins, and it also exhibited thermal irreversibility at higher MTGase concentrations. When applied to minced pork gel, the type-E gelatin crosslinked by MTGase improved water-holding capacity and texture properties, reducing cooking loss.
Correction
Nanoscience & Nanotechnology
Nuseibah AL Qtaish, Idoia Gallego, Alejandro J. Paredes, Ilia Villate-Beitia, Cristina Soto-Sanchez, Gema Martinez-Navarrete, Myriam Sainz-Ramos, Tania B. Lopez-Mendez, Francisco Javier Chichon, Noelia Zamarren, Eduardo Fernandez, Gustavo Puras, Jose Luis Pedraz
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Rosa F. Monteiro, Syeda M. Bakht, Manuel Gomez-Florit, Fernanda C. Stievani, Ana L. G. Alves, Rui L. Reis, Manuela E. Gomes, Rui M. A. Domingues
Summary: The authors propose an automated 3D printing method to create tendon microphysiological systems (MPSs) embedded in a biomimetic fibrillar support platform. They use tendon decellularized extracellular matrix (dECM) to formulate bioinks that closely mimic the biochemical characteristics of tendon niche. They successfully recreate the cellular patterns and phenotype of the tendon core and study the interaction between different cell populations. The proposed concept shows promise for the automated fabrication of organotypic tendon-on-chip models, providing a valuable tool for studying tendon physiology, pathogenesis mechanisms, and testing new treatments for tendinopathies.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Editorial Material
Engineering, Environmental
Unax Lertxundi, Saioa Domingo-Echaburu, Susana Barros, Miguel Machado Santos, Teresa Neuparth, Jose Benito Quintana, Rosario Rodil, Rosa Montes, Gorka Orive
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ana L. Graca, Rui M. A. Domingues, Manuel Gomez-Florit, Manuela E. Gomes
Summary: In this study, the role of platelet-derived extracellular vesicles (EVs) in stem cells' tenogenic commitment was assessed using a 3D bioengineered in vitro tendon model. The findings showed that platelet-derived EVs boosted the tenogenic commitment of stem cells and enhanced the deposition of tendon-like extracellular matrix. These results suggest that platelet-derived EVs have promising applications in tissue engineering and regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Esteban Astudillo-Ortiz, Pedro S. Babo, Pia T. Sunde, Kerstin M. Galler, Manuel Gomez-Florit, Manuela E. Gomes
Summary: In recent years, there has been a paradigm shift in the endodontic field towards regenerative therapies for the pulp-dentin complex. However, the abundant literature on this subject is scattered and lacks consensus in terminology and concepts. This literature review aims to clarify the commonly used terminology, summarize the main research areas, and identify future trends in pulp regeneration.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Fahimeh Roshanfar, Saeed Hesaraki, Alireza Dolatshahi-Pirouz, Mohsen Saeidi, Sara Leal-Marin, Birgit Glasmacher, Gorka Orive, Sajjad Khan Einipour
Summary: Calcium phosphate cements (CPCs) are promising for bone defect treatment due to their properties, however their brittleness limits their application. This study investigated the use of hybrid silk fibroin (SF) and kappa-carrageenan (k-CG) nanofibers as reinforcements in CPC. The addition of nanofibers improved the mechanical properties and cell mineralization of CPC composites, indicating their potential for bone cell regeneration.
Editorial Material
Environmental Sciences
Iker Egana, Saioa Domingo-Echaburu, Gorka Orive, Unax Lertxundi
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Oncology
Ali Zarrabi, David Perrin, Mahboubeh Kavoosi, Micah Sommer, Serap Sezen, Parvaneh Mehrbod, Bhavya Bhushan, Filip Machaj, Jakub Rosik, Philip Kawalec, Saba Afifi, Seyed Mohammadreza Bolandi, Peiman Koleini, Mohsen Taheri, Tayyebeh Madrakian, Marek J. Los, Benjamin Lindsey, Nilufer Cakir, Atefeh Zarepour, Kiavash Hushmandi, Ali Fallah, Bahattin Koc, Arezoo Khosravi, Mazaher Ahmadi, Susan Logue, Gorka Orive, Stevan Pecic, Joseph W. Gordon, Saeid Ghavami
Summary: Rhabdomyosarcoma is a rare cancer that affects skeletal muscle, primarily in children and young adults. The treatment strategies for this disease include multi-agent chemotherapies following surgical resection and radiotherapy. This review provides a detailed clinical understanding of rhabdomyosarcoma, including its classification, diagnosis, and treatment strategies. The impact of apoptosis, macro-autophagy, and the unfolded protein response on chemotherapy response is discussed. Additionally, the use of in vivo mouse and zebrafish models and in vitro three-dimensional bioengineering models for screening future therapeutic approaches and promoting muscle regeneration is explored.
Article
Pharmacology & Pharmacy
Fatima Garcia-Villen, Idoia Gallego, Myriam Sainz-Ramos, Jorge Ordoyo-Pascual, Sandra Ruiz-Alonso, Laura Saenz-del-Burgo, Conor O'Mahony, Jose Luis Pedraz
Summary: Drug adherence is a significant issue in medical treatment of chronic diseases like rheumatoid or psoriatic arthritis. Treated with monoclonal antibodies, the use of auto-injectors as delivery devices improves drug adherence due to their user-friendliness. This study investigated the feasibility of including monoclonal antibodies in auto-injector pens and evaluated the drug stability after concentration and freeze-drying, finding that mannitol improved stability but further investigation is required for a domain instability. These results contribute to advancing drug delivery systems for better patient experience and drug adherence.
Article
Pharmacology & Pharmacy
Jose David Celdran, Lawrence Humphreys, Desiree Gonzalez, Cristina Soto-Sanchez, Gema Martinez-Navarrete, Ivan Maldonado, Idoia Gallego, Ilia Villate-Beitia, Myriam Sainz-Ramos, Gustavo Puras, Jose Luis Pedraz, Eduardo Fernandez
Summary: Gene therapy and optogenetics show promise for treating nervous system pathologies, but viral vectors used in these approaches pose risks. Non-viral nanoparticles such as niosomes offer a potential alternative, and our study tested three niosome formulations with optogenetic plasmids in rat cortical neurons. We found successful expression of optogenetic channels, but observed changes in cell morphology and electrophysiological properties, particularly at higher concentrations of niosomes. Our results highlight the potential and the need for careful optimization in the use of niosomes for optogenetic applications.
Review
Materials Science, Biomaterials
M. Ozgen Oztuerk-Oncel, Baltazar Hiram Leal-Martinez, Rosa F. Monteiro, Manuela E. Gomes, Rui M. A. Domingues
Summary: Embedded 3D bioprinting systems provide a stable, cell-friendly, and perfusable environment for accurately replicating the architecture and function of native tissues. Support baths further serve as functional housing systems for long-term maintenance and screening. This minireview summarizes recent advances in embedded 3D bioprinting and highlights its potential for automated fabrication of more biomimetic in vitro models.
BIOMATERIALS SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Giovanna Revilla, Nuseibah Al Qtaish, Pablo Caruana, Myriam Sainz-Ramos, Tania Lopez-Mendez, Francisco Rodriguez, Veronica Paez-Espinosa, Changda Li, Nuria Fucui Vallverdu, Maria Edwards, Antonio Moral, Jose Ignacio Perez, Juan Carlos Escola-Gil, Jose Luis Pedraz, Idoia Gallego, Rosa Corcoy, Maria Virtudes Cespedes, Gustavo Puras, Eugenia Mato
Summary: This study produced and assessed lenvatinib-loaded PLGA nanoparticles (NPs) as a novel therapeutic approach for aggressive thyroid tumors. The decorated NPs showed better cytotoxicity and therapeutic efficacy in vitro and in vivo compared to NPs without decoration, suggesting a promising strategy for targeted and effective administration of TKIs.
Meeting Abstract
Cell & Tissue Engineering
Manuel Gomez-Florit, Adriana Vilaca, Hanna Tiainen, Rui M. A. Domingues, Barbara B. Mendes, Alejandro Barrantes, Rui L. Reis, Manuela E. Gomes
TISSUE ENGINEERING PART A
(2022)
