Article
Chemistry, Analytical
Lihong Su, Liangfen Cheng, Qin Mi, Zhenmin Liu, Juan Zhang
Summary: In this work, a pH-adjusted self-assembled lipid bilayer was proposed as a signal output mode for the analysis of total N-glycolyneaurnaminic acids (TNeu5Gc). A ratiometric electrochemical biosensor was constructed to analyze TNeu5Gc, showing a wide linear detection range and low detection limit. This work not only establishes a ratiometric electrochemical biosensor for TNeu5Gc analysis, but also provides insights for self-assembly of lipid bilayers in response to pH changes.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Chan Ho Park, Ian A. P. Thompson, Sharon S. Newman, Linus A. Hein, Xizhen Lian, Kaiyu X. Fu, Jing Pan, Michael Eisenstein, H. Tom Soh
Summary: Cells rely on secreted signaling molecules to coordinate essential biological functions, but direct measurement of these signals is challenging. This study presents an aptamer-conjugated hydrogel matrix that enables real-time measurement of specific secreted analytes. By monitoring inter-cellular cAMP signals in amoeba cells, the researchers achieved direct measurements of oscillatory cAMP signaling. This approach can be generalized to visualize diverse extracellular signaling processes and their effects on recipient cells.
ADVANCED MATERIALS
(2023)
Review
Polymer Science
Incherah Bekkouche, Alexander Y. Y. Shishonin, Alexandre A. A. Vetcher
Summary: The structure of triple-stranded DNA, consisting of three oligonucleotide chains, is explored. Hoogsteen base pairing is described as a mechanism for forming triple-stranded DNA. The involvement of these structures in various diseases is discussed. The focus of the review is on the development of biomedical oligonucleotides with triple-stranded DNA.
Review
Chemistry, Analytical
Dongmin Kim, Seungmin Yoo
Summary: Quantum dots (QDs) are a promising new generation of luminophores with tunable photoluminescence and photochemical stability. The development of aptamer-functionalized QD biosensors has enabled highly sensitive detection of molecules in clinical and environmental settings, with recent advances focusing on optical signal enhancement and quenching strategies. Potential strategies for further expanding the practical applications of biosensors across various scientific and technological fields are also discussed.
Review
Biochemistry & Molecular Biology
Samin Nooranian, Arash Mohammadinejad, Taraneh Mohajeri, Ghazaleh Aleyaghoob, Reza Kazemi Oskuee
Summary: Aptamer-conjugated gold nanoparticles exhibit advantages such as high binding affinity, high biocompatibility, enhanced target selectivity, and long circulatory half-life, making them widely used in biosensing systems as well as personalized medicine, disease marker detection, food safety, and more.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Physical
Bihai Ye, Jian Yao, Changhui Wu, Huilong Zhu, Weijun Yao, Lili Jin, Xiaowei Dou
Summary: In this study, a new rhodium catalyst was developed for the asymmetric conjugate pyridylation reaction with high yields and good enantioselectivities. By optimizing the catalyst and solvent conditions, the challenges of the pyridylation reaction were successfully overcome.
Article
Chemistry, Multidisciplinary
X. Liu, J. Song, B. Yin, T. Jiang, H. Pang, L. Yang, S. Wu
Summary: In this study, a powerless, gas-permeable, flexible and transparent ascorbic acid biosensor based on carbon micromesh electrode was developed. The biosensor showed excellent performances including high transmittance, wide detection range, low limit of detection, good selectivity, and long-term storage stability. By integrating a pH-sensing electrode, the analysis of ascorbic acid can be corrected. The biosensor demonstrated good sensitivity and practicability towards wearable electronics in the detection of artificial tears.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Yuto Honda, Takahiro Nomoto, Makoto Matsui, Hiroyasu Takemoto, Yutaka Miura, Nobuhiro Nishiyama
Summary: The construction of a systemically applicable beta-Gal-loaded ternary complex through sequential self-assembly in aqueous solution was reported. The complex protected the loaded beta-Gal from degradation and exhibited enhanced and selective enzymatic activity at the tumor site. This novel approach may offer a new strategy for enzyme-prodrug theragnosis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Chemical
Roopkumar Sangubotla, Jongsung Kim
Summary: In this study, a selectively recognizable fluorometric sensor based on carbon-rich zinc oxide-derived nano-structures (ZnO NSs) was developed for detecting the biomarker Neu5Ac in neurological disorders. The sensor showed high sensitivity and efficiency, with the potential for in vitro and in vivo detection of diverse neurological biomarkers.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Nanoscience & Nanotechnology
Hui Zhang, Cheng Jin, Lin Zhang, Bo Peng, Yibin Zhang, Yan Liu, Ling Li, Mao Ye, Wei Xiong, Weihong Tan
Summary: The study developed a CD71-specific aptamer targeting the XQ-2d-MMAE conjugate that can distinguish UM cells from normal human uveal melanocytes, suggesting it as a promising novel anti-tumor agent for UM treatment. It demonstrated that CD71 is a reliable target for drug delivery in UM and could be utilized as a model for exploring aptamer-mediated targeted UM treatment strategies.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Yukichi Horiguchi, Kevin Barthelmes, Yuji Miyahara, Akira Matsumoto
Summary: The detection and isolation of sialic acid (SA) over-expressed biomolecules remains a focus in cancer research. Boronic acid-based SA-recognition chemistry offers a synthetic molecular basis for such techniques. The derivative 5-Boronopicolinic acid, known for its high SA-binding strength, was immobilized on a gold surface and showed submicron-molar level sensitivity in its interaction with the glycoprotein fetuin implicated in cancers.
Article
Chemistry, Analytical
Hui Yue, Jinri Chen, Xu Chen, Xiaoli Wang, Yuting Zhang, Nandi Zhou
Summary: This study utilized different magnetic beads-based SELEX methods to screen aptamers specific for Neu5Ac, and optimized the best aptamer through molecular simulation. A fluorescent biosensor constructed showed good specificity and interferents-resistance.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Xiaoqi Chen, Aiyou Huang, Peng Ju
Summary: The integration of sterilization capabilities into sensing platforms is crucial for mitigating health risks associated with pathogenic bacteria. This study developed a photoelectrochemical bacterial sensing platform with sterilization function, improving performance through the incorporation of PTBA and AuNPs. The platform selectively captured bacteria and demonstrated high sensitivity detection and effective antibacterial properties.
MICROCHEMICAL JOURNAL
(2024)
Article
Spectroscopy
Fang Mi, Ming Guan, Ying Wang, Guotong Chen, Pengfei Geng, Qiuling Cui, Hongtao Huan
Summary: We present a cost-effective and highly sensitive label-free SERS biosensor for pathogen detection. The biosensor utilizes 4-formylphenylboric acid functionalized magnetic nanoparticles to adsorb pathogenic bacteria and aptamer modified Au@AgNPs as SERS substrate for specific binding. The detection of pathogenic bacteria is achieved using a portable Raman spectrometer and analyzed through principal component analysis (PCA). The SERS detection limit for Staphylococcus aureus is 34 CFU/mL, and the linear range for Escherichia coli is 102-106 CFU/mL.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Christopher M. Gabriel, Brian R. Pimentel, Christian A. Gomez, Isaiah Cedillo, Andrew A. Rodriguez
Summary: This study demonstrates the enhanced purification of GalNAc-conjugated oligonucleotides using boronic acids as mobile phase additives during reversed-phase chromatography. The boronic acid additives work by increasing the hydrophobicity and affinity of the conjugate molecules for the stationary phase. The best performing boronic acids were PBA at pH 8, 2-MePBA at pH 9, and 2-CF(3)PBA at pH >= 10.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Article
Chemistry, Analytical
Anzhi Sheng, Lihong Su, Jiayi Wang, Tianxiang Xue, Pei Wang, Juan Zhang
ANALYTICA CHIMICA ACTA
(2020)
Review
Chemistry, Analytical
Pei Wang, Tianxiang Xue, Anzhi Sheng, Liangfen Cheng, Juan Zhang
Summary: This review summarizes the roles of chemoselective ligations in biosensor construction and reviews their applications in detecting various targets. Furthermore, the future development trends of these reactions in biosensing are discussed.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Tianxiang Xue, Xiquan Yue, Jiayi Wang, Anzhi Sheng, Pei Wang, Juan Zhang
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Analytical
Anzhi Sheng, Pei Wang, Jingyi Yang, Longfei Tang, Feng Chen, Juan Zhang
Summary: In this study, it was found that MXene can efficiently adsorb single-stranded DNA and be coupled with CRISPR-Cas12a for sensitive detection of LPS and bacteria. Through carefully designed aptamers, the trans-cleavage activity of CRISPR-Cas12a is activated, allowing for selective and sensitive quantification of targets in different samples. This method provides a new direction for the exploration of universal analytical methods based on MXene coupled with CRISPR-Cas12a.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Tianxiang Xue, Longfei Tang, Xiquan Yue, Lili Niu, Jinhui Peng, Juan Zhang
Summary: A chemoselective ligation assisted DNA walker with input and output of double signals has been developed using oxime chemistry and alkyne-azide cycloaddition. This biosensor, designed for lipopolysaccharide (LPS) and 5-hydroxymethyl-2-furaldehyde (HMF) targets, can sensitively differentiate and analyze both targets with one-to-one mapping functionality. This work offers a new perspective on analyzing double targets based on chemoselective ligation assisted DNA walker.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Chemistry, Analytical
Pei Wang, Longfei Tang, Bohui Zhou, Liangfen Cheng, Robert Chunhua Zhao, Juan Zhang
Summary: Immune checkpoints are a series of molecules that regulate the degree of immune activation, including PD-1/PD-L1, CTLA-4, LAG-3, and others. Immune checkpoint therapy is considered to be a safer and more effective treatment for tumor immunotherapy compared to conventional therapy. The analysis of immune checkpoints is crucial for immunotherapy medication, treatment, and prognosis evaluation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Liangfen Cheng, Fuhan Yang, Longfei Tang, Lelin Qian, Xu Chen, Feng Guan, Juan Zhang, Genxi Li
Summary: This study explores the use of cellular interface supported CRISPR/Cas trans-cleavage for electrochemical simultaneous detection of two types of sialic acids, Neu5Gc and Neu5Ac, which is important for evaluating tumor development. The method is simple, can quantitatively analyze sialic acids on cell surface, and can be used for the identification of bladder cancer cells.
Article
Chemistry, Analytical
Anzhi Sheng, Jingyi Yang, Liangfen Cheng, Juan Zhang
Summary: In this work, boronic ester-mediated dual recognition coupled with a CRISPR/Cas12a system has been proposed for highly specific and sensitive detection of lipopolysaccharide (LPS). The method utilizes boronic acid and LPS aptamer (LPSA) for simultaneous recognition, and CRISPR/Cas12a for signal amplification. The method has a linear detection range from 0.05 to 5000 ng/mL with a lowest detection limit of 44.86 pg/mL, and can be developed as a universal platform for the analytical detection of other carbohydrates.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Dongsheng Mao, Yifei Li, Cuicui Lu, Xiaohao Liu, Anzhi Sheng, Kun Han, Xiaoli Zhu
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Xu Chen, Xianyong Wu, Ziyu He, Juan Zhang, Ya Cao, Dongsheng Mao, Chang Feng, Bo Tian, Guifang Chen
NEW JOURNAL OF CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Anzhi Sheng, Lihong Su, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Juan Zhang
CHEMICAL COMMUNICATIONS
(2020)
