Article
Plant Sciences
Cai Zhang, Jin-Quan Zhao, Jia-Xing Sun, Hui-Jun Li
Summary: The study revealed that the hepatotoxicity induced by psoralen and isopsoralen is associated with the expression and activity of CYP1A2, possibly through the mechanism of AhR-mediated transcriptional induction of CYP1A2. These findings are important to alert about the potential risks of combined usage of psoralen or isopsoralen with AhR ligands or CYP1A2 substrates in clinical practice.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Benyu Liu, Shiming Fang, Kun Zhou, Lulu Ma, Yaling Shi, Yuefei Wang, Xiumei Gao
Summary: This study aimed to reveal the hepatotoxicity distinction of coumarin-related compounds from glycosides to aglycones in Fructus Psoraleae (FP) and elucidate their potential mechanism. The results showed that P, IP, FA, and IFA were identified as hepatotoxic compounds, while their glycosides were almost non-hepatotoxic. The conversion from glycosides to aglycones played an essential role in FP-induced hepatotoxicity.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Cai Zhang, Song Fan, Jin-Quan Zhao, Yan Jiang, Jia-Xing Sun, Hui-Jun Li
Summary: The purpose of this study was to investigate the mechanism underlying psoralen/isopsoralen-induced hepatotoxicity. Through transcriptomic and metabolomic analyses, xenobiotic metabolism via cytochromes P450 was identified as a potential pathway. Further validation identified CYP1A2 as the key metabolic enzyme. Induction of CYP1A2 aggravated hepatotoxicity, while inhibition alleviated the toxic effects. Glutathione adducts with reactive intermediates of psoralen/isopsoralen were detected, suggesting that CYP1A2-mediated metabolic activation is responsible for the observed hepatotoxicity.
PHYTOTHERAPY RESEARCH
(2023)
Article
Plant Sciences
Ning Zhou, Zhe Li, Jin-Jin Wang, Qi-Tong Wu, Kai Li, Xiao-Ke Zheng, Wei-Sheng Feng
Summary: The study investigated the compatibility and mechanism of salt-processed Psoraleae Fructus and salt-processed Foeniculi Fructus, and found that salt-processing affects the chemical components and critical metabolites, leading to both positive and negative effects on efficacy. This novel approach provided insights into the compatibility mechanism of herbs for treating diarrhoea.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Longlong Xu, Xianglin Tang, Feiran Hao, Yue Gao
Summary: This study investigated the toxic effect of Fructus Psoraleae (FP) using a metabolomics approach and found that long-term exposure to high-dose ethanol extract of FP (EEFP) may cause hepatorenal toxicity in rats. The serum metabolomics approach can improve the diagnostic efficiency of FP toxicity.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Plant Sciences
Chen Gao, Chang Liu, Yuanyuan Wei, Qingtao Wang, Xuan Ni, Shaofeng Wu, Yizhuo Fang, Zhihui Hao
Summary: This study identified 107 compounds in the ethanol extract of salt-processed Psoraleae Fructus (EEPF) using UHPLC-HRMS analysis. Acute oral toxicity test in mice showed that the LD50 of EEPF was 15.95 g/kg. The liver and kidney were identified as the main target toxic organs of EEPF, causing liver injury through oxidative stress and pyroptotic damage via the NLRP3/ASC/Caspase-1/GSDMD signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Toxicology
Juyang Huang, Qin Wang, Mengying Chen, Yanan Bi, Hong Shi, Kun Zhou
Summary: This study demonstrated that psoralen induced hepatotoxicity in rats, possibly through affecting bile acid transporters and leading to the disorder of bile acid transport and accumulation in hepatocytes.
HUMAN & EXPERIMENTAL TOXICOLOGY
(2021)
Article
Food Science & Technology
Shuaishuai Chen, Weiyu Guo, Huan Liu, Jiang Zheng, Dingyan Lu, Jia Sun, Chun Li, Chunhua Liu, Yonglin Wang, Yong Huang, Wen Liu, Yongjun Li, Ting Liu
Summary: Psoralen and isopsoralen are major components responsible for Psoraleae Fructus-induced hepatotoxicity. This study reveals that their cytotoxic effects are mediated by metabolic activation through cytochrome P450 (CYP) enzymes. Specific CYP enzymes, such as CYP2C9, 2C19, 2D6, and 2E1, play a crucial role in the metabolic activation of psoralen and isopsoralen. The activation of these compounds by CYP enzymes regulates core targets and signaling pathways, resulting in cytotoxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemical Research Methods
Xiao-yan Wu, Li-juan Xie, Jun-jie He, Xing-xu Yan, Fang-fang Zhang, Yan-yan Xu, Yu-bo Li
Summary: In this study, the lipidomics changes in rats treated with Fructus Psoraleae (FP) decoction were investigated. Differential lipid metabolites related to hepatotoxicity in rats with kidney-yin deficiency were identified, and the metabolism of glycerophospholipid and sphingolipid was found to be strongly related to the mechanism of hepatotoxicity induced by FP.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jiali Wang, Zefei Jia, Wen Pan, Jing Hu
Summary: This research investigated the harmful impacts and underlying mechanisms of Crotonis Fructus (CF), a traditional poisonous laxative. The study found that CF induced hepatic and gastrointestinal damage, disrupted gut microbiota composition, and disturbed metabolites. The results provide a new understanding of toxic traditional medicine.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Yan Wang, Guang Xu, Zhilei Wang, Ruisheng Li, Xiaoyan Zhan, Hongbin Liu, Qin Qin, Weixia Li, Xiaoyan Wang, Mingliang Zhang, Jinfa Tang, Zhaofang Bai, Xiaohe Xiao
Summary: This study found that psoralidin from Psoraleae Fructus induces idiosyncratic drug-induced liver injury (IDILI) by activating inflammasomes. The results suggest that psoralidin can activate not only the NLRP3 inflammasome but also other types of inflammasomes. The study further revealed the mechanism of liver injury induced by psoralidin.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lang Yan, Fangyuan Gao, Wenjing Shi, Bijiang Geng, Jiqianzhu Zhang, Jingjing Mao, Yijun Tian, Lijun Ren, Xiaoyu Dai, Jikuai Chen, Jiangbo Zhu, Xiaofang Zhang
Summary: High-dose lanthanum nitrate had an effect on the liver weight and organ weight of SD rats, but there was no liver toxicity and no significant reproductive toxic effects.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Zhaojuan Guo, Pin Li, Chunguo Wang, Qianjun Kang, Can Tu, Bingqian Jiang, Jingxuan Zhang, Weiling Wang, Ting Wang
Summary: This study found that the ethanol extraction process aggravated the hepatotoxicity of Psoraleae Fructus, with bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol identified as the main toxic ingredients. These constituents induced cell apoptosis, increased lipid accumulation and reactive oxygen species levels, while decreasing mitochondrial membrane potential. The mechanism may be associated with oxidative stress and mitochondrial damage-mediated apoptosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jianfu Wu, Yijun Chen, Jinxuan Zhang, Jinghuan Cheng, Yongde Chen, Tao Wu, Min Zhang
Summary: Sea cucumber peptides (SCP) were found to inhibit bleomycin-induced pulmonary fibrosis in SD rats by reducing inflammatory cytokines and improving lung tissue structure, with potential therapeutic effects on weight loss. Metabolomics analysis revealed significant downregulation of several key metabolic factors in lung tissue, suggesting SCP's protective role in pulmonary fibrosis. The study also highlighted the lysosome pathway and d-mannose as crucial mechanisms of action for SCP in treating pulmonary fibrosis.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Integrative & Complementary Medicine
Ming-Liang Zhang, Xu Zhao, Wei-Xia Li, Xiao-Yan Wang, Ming Niu, Hui Zhang, Yu-Long Chen, De-Xin Kong, Yuan Gao, Yu-Ming Guo, Zhao-Fang Bai, Yan-Ling Zhao, Jin-Fa Tang, Xiao-He Xiao
Summary: Psoralea corylifolia Linn. (BGZ) has different effects on liver protection and damage in rats with kidney-yang deficiency syndrome and kidney-yin deficiency syndrome. The transcriptomics and metabonomics analysis revealed that BGZ affects different metabolic pathways in these two syndrome models. It is important to consider the predisposed populations when using drugs related to energy metabolism.