期刊
MICROORGANISMS
卷 7, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms7110549
关键词
Acinetobacter; 16S rRNA; gyrB; rpoB; genome sequence; ANI; in silico DDH; Clarias microcephalus; probiotics; antagonists; Acb complex
类别
资金
- Thailand Research Fund (TRF)
- Betagro Science Center, Thailand [DPG5980003]
The bacterial strain KU011TH was isolated from the skin mucus of healthy bighead catfish. The strain is a Gram-negative coccobacillus that is nonmotile, aerobic, catalase positive, oxidase negative, and nonhemolytic. Sequence analyses of the housekeeping genes 16S rRNA, gyrB and rpoB indicate that this strain is a new member of the Acb complex of the genus Acinetobacter and is closely related to Acinetobacter pittii and Acinetobacter lactucae. In addition, the genome relatedness-associated ANIb (<95-96%) and in silico DDH (<70%) values clearly supported the new member of the genus Acinetobacter and the Acb complex. The genome of the strain KU011TH was approximately 3.79 Mbp in size, comprising 3619 predicted genes, and the DNA G+C content was 38.56 mol%. The major cellular fatty acids were C18:1 omega 9c, C16:0, C16:1, C20:2, C18:2 omega 6c and C18:1 omega 9t. The whole-genome sequences and phenotypic, phylogenetic, and chemotaxonomic data clearly support the classification of the strain KU011TH as a new member in the genus Acinetobacter which is closest to A. pittii. Additionally, the new bacterial strain exhibited strong activity against a broad range of freshwater fish pathogens in vitro.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据