Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems

A simple, robust, and low-cost method to produce the PURE cell-free system.

(2019) Barbora Lavickova et al.   ACS Synthetic Biology

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

(2019) Max Z. Levine et al.   Jove-Journal of Visualized Experiments

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

(2019) Daniel J. Wiegand et al.   Jove-Journal of Visualized Experiments

Application of Cell-Free Protein Synthesis for Faster Biocatalyst Development

(2019) Jascha Rolf et al.   Catalysts

Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate

(2019) Giada Lo Gullo et al.   ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL

Golgi-on-a-Chip for the Cell-Free Bio-Nanomanufacturing of Protein Therapeutics

(2019) Susan Daniel et al.   BIOPHYSICAL JOURNAL

Cell-Free Protein Synthesis: Chassis toward the Minimal Cell

(2019) Ke Yue et al.   Cells

Cell-free soluble expression of the membrane protein PsbS

(2019) M. Krishnan et al.   PROTEIN EXPRESSION AND PURIFICATION

An equation for biomimicking macromolecular crowding using Escherichia coli MG1655 strain

(2019) Khushal Khambhati et al.   BIOPHYSICAL CHEMISTRY

Endotoxin-free E. coli -based cell-free protein synthesis: Pre-expression endotoxin removal approaches for on-demand cancer therapeutic production

(2018) Kristen M. Wilding et al.   Biotechnology Journal

Synthetic small RNAs: Current status, challenges, and opportunities

(2018) Shreya Patel et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Next-generation biocontainment systems for engineered organisms

(2018) Jeong Wook Lee et al.   Nature Chemical Biology

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

(2018) Rey W. Martin et al.   Nature Communications

Endotoxin-free E. coli -based cell-free protein synthesis: Pre-expression endotoxin removal approaches for on-demand cancer therapeutic production

(2018) Kristen M. Wilding et al.   Biotechnology Journal

Cell-free protein synthesis for producing ‘difficult-to-express’ proteins

(2018) Xing Jin et al.   BIOCHEMICAL ENGINEERING JOURNAL

Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens

(2018) Benjamin J. Des Soye et al.   ACS Synthetic Biology

Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications

(2018) Wan-Qiu Liu et al.   BIOCHEMICAL ENGINEERING JOURNAL

Current progress in CRISPR-based diagnostic platforms

(2018) Khushal Khambhati et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Exploring the potential of genome editing CRISPR-Cas9 technology

(2017) Vijai Singh et al.   GENE

Recent Advances in CRISPR-Cas9 Genome Editing Technology for Biological and Biomedical Investigations

(2017) Vijai Singh et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

(2017) Mark Rustad et al.   Jove-Journal of Visualized Experiments

High-yield production of “difficult-to-express” proteins in a continuous exchange cell-free system based on CHO cell lysates

(2017) Lena Thoring et al.   Scientific Reports

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

The therapeutic potential of insulin-like growth factor-1 in central nervous system disorders

(2016) Jesse Costales et al.   NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS

Design and synthesis of a minimal bacterial genome

(2016) C. A. Hutchison et al.   SCIENCE

High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format

(2016) Robert B. Quast et al.   Scientific Reports

In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis

(2016) Anthony W. Goering et al.   ACS Synthetic Biology

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

(2016) Emanuel G. Worst et al.   Jove-Journal of Visualized Experiments

Recombinant human granulocyte macrophage colony stimulating factor (hGM-CSF): Possibility of nanoparticle-mediated delivery in cancer immunotherapy

(2016) Selvarajan Vanitha et al.   Bioengineered

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing

(2015) Helmut Merk et al.   BIOLOGICAL CHEMISTRY

Cell-free expression with the toxic amino acid canavanine

(2015) Emanuel G. Worst et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Different applications of virus-like particles in biology and medicine: Vaccination and delivery systems

(2015) Zeinab Shirbaghaee et al.   BIOPOLYMERS

Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system

(2015) Yonatan Chemla et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

Improving Cell-Free Protein Synthesis through Genome Engineering ofEscherichia coliLacking Release Factor 1

(2015) Seok Hoon Hong et al.   CHEMBIOCHEM

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

(2015) Anne Zemella et al.   CHEMBIOCHEM

Repurposing the translation apparatus for synthetic biology

(2015) Benjamin J Des Soye et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Characterizing and prototyping genetic networks with cell-free transcription–translation reactions

(2015) Melissa K. Takahashi et al.   METHODS

Recoded organisms engineered to depend on synthetic amino acids

(2015) Alexis J. Rovner et al.   NATURE

The PURE system for the cell-free synthesis of membrane proteins

(2015) Yutetsu Kuruma et al.   Nature Protocols

The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA

(2015) Weijun Zhu et al.   Retrovirology

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

(2015) Amin S. M. Salehi et al.   Biotechnology Journal

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

(2015) Yu Hisano et al.   Scientific Reports

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch

(2014) Yuki Soma et al.   METABOLIC ENGINEERING

A versatile framework for microbial engineering using synthetic non-coding RNAs

(2014) Lei S. Qi et al.   NATURE REVIEWS MICROBIOLOGY

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

(2014) F. Port et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems

(2014) Melissa K. Takahashi et al.   ACS Synthetic Biology

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

(2014) Xingjie Ren et al.   Cell Reports

Optimizing Metabolite Production Using Periodic Oscillations

(2014) Steven W. Sowa et al.   PLoS Computational Biology

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advancements in synthetic biology: Current status and challenges

(2013) Vijai Singh   GENE

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

(2013) Dokyun Na et al.   NATURE BIOTECHNOLOGY

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates

(2013) Prashanta Shrestha et al.   New Biotechnology

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

(2013) Cem Albayrak et al.   NUCLEIC ACIDS RESEARCH

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines

(2013) Y. Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants

(2013) D. L. Shis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation

(2013) Seok Hoon Hong et al.   ACS Synthetic Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

(2013) Zachary Z. Sun et al.   Jove-Journal of Visualized Experiments

Cell-free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens

(2012) John P. Welsh et al.   BIOTECHNOLOGY AND BIOENGINEERING

Lipids at the interface of virus–host interactions

(2012) Vineela Chukkapalli et al.   CURRENT OPINION IN MICROBIOLOGY

Genetic programs constructed from layered logic gates in single cells

(2012) Tae Seok Moon et al.   NATURE

‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules

(2012) Hwangbeom Kim et al.   NUCLEIC ACIDS RESEARCH

Multiplexed in Vivo His-Tagging of Enzyme Pathways for in Vitro Single-Pot Multienzyme Catalysis

(2012) Harris H. Wang et al.   ACS Synthetic Biology

Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction

(2012) Jonghyeon Shin et al.   ACS Synthetic Biology

Proteases as therapeutics

(2011) Charles S. Craik et al.   BIOCHEMICAL JOURNAL

Surface Functionalization of Virus-Like Particles by Direct Conjugation Using Azide−Alkyne Click Chemistry

(2011) Kedar G. Patel et al.   BIOCONJUGATE CHEMISTRY

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient disulfide bond formation in virus-like particles

(2011) Bradley C. Bundy et al.   JOURNAL OF BIOTECHNOLOGY

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

The anti-cancer activity of human consensus interferon-alpha synthesized in cell-free system

(2011) Nawal Abd EL-Baky et al.   PROTEIN EXPRESSION AND PURIFICATION

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Efficacy of a Potential Trivalent Vaccine Based on Hc Fragments of Botulinum Toxins A, B, and E Produced in a Cell-Free Expression System

(2010) R. Zichel et al.   Clinical and Vaccine Immunology

Virus-like particles in vaccine development

(2010) António Roldão et al.   Expert Review of Vaccines

A designer network coordinating bovine artificial insemination by ovulation-triggered release of implanted sperms

(2010) Christian Kemmer et al.   JOURNAL OF CONTROLLED RELEASE

A tunable dual-promoter integrator for targeting of cancer cells

(2010) Lior Nissim et al.   Molecular Systems Biology

Robust multicellular computing using genetically encoded NOR gates and chemical ‘wires’

(2010) Alvin Tamsir et al.   NATURE

A synchronized quorum of genetic clocks

(2010) Tal Danino et al.   NATURE

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

(2010) Sriram Kosuri et al.   NATURE BIOTECHNOLOGY

Synthetic biology: applications come of age

(2010) Ahmad S. Khalil et al.   NATURE REVIEWS GENETICS

Genetic control of mammalian T-cell proliferation with synthetic RNA regulatory systems

(2010) Y. Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming Cellular Behavior with RNA Controllers Responsive to Endogenous Proteins

(2010) S. J. Culler et al.   SCIENCE

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

(2010) D. G. Gibson et al.   SCIENCE

The wheat germ cell-free protein synthesis system: A key tool for novel malaria vaccine candidate discovery

(2009) Takafumi Tsuboi et al.   ACTA TROPICA

Acute stroke therapy with tissue plasminogen activator (tPA) since it was approved by the U.S. Food and Drug Administration (FDA)

(2009) Justin A. Zivin   ANNALS OF NEUROLOGY

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

High yield cell-free production of integral membrane proteins without refolding or detergents

(2008) Jessica J. Wuu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

In situ synthesis of protein arrays

(2008) Mingyue He et al.   CURRENT OPINION IN BIOTECHNOLOGY

Wheat Germ Cell-Free System-Based Production of Malaria Proteins for Discovery of Novel Vaccine Candidates

(2008) T. Tsuboi et al.   INFECTION AND IMMUNITY

A fast, robust and tunable synthetic gene oscillator

(2008) Jesse Stricker et al.   NATURE

Human protein factory for converting the transcriptome into an in vitro–expressed proteome

(2008) Naoki Goshima et al.   NATURE METHODS

Higher-Order Cellular Information Processing with Synthetic RNA Devices

(2008) M. N. Win et al.   SCIENCE

Escherichia coli-based cell-free synthesis of virus-like particles

(2007) Bradley C. Bundy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Streamlining Escherichia Coli S30 Extract Preparation for Economical Cell-Free Protein Synthesis

(2005) David V. Liu et al.   BIOTECHNOLOGY PROGRESS