Review
Biochemistry & Molecular Biology
Yasunari Matsuzaka, Ryu Yashiro
Summary: Extracellular vesicles (EVs) are lipid bilayer membrane particles that play critical roles in intracellular communication. Mesenchymal stem cells (MSCs) induce tissue regeneration by direct recruitment into injured tissues or secreting factors. Recent studies have shown that MSC-derived EVs have regenerative effects against various diseases and can serve as drug delivery systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Vishnu Priya Murali, Christina A. Holmes
Summary: This review summarizes various biomaterial-based extracellular vesicle (EV) delivery systems used in preclinical tissue regeneration studies and other therapeutic applications. EV-based therapies have shown success in preclinical models for biomedical applications, but the challenge lies in prolonging EV retention at target sites for improved therapeutic efficacy through biomaterial-based systems.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Ngoc Phung Ly, Hwa Seung Han, Myungsuk Kim, Jae Hyung Park, Ki Young Choi
Summary: This article provides a comprehensive introduction to plant-derived vesicles (PDVs), including their biological characteristics, purification methods, and a summary of their current development potential for cancer therapy.
BIOACTIVE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Lalithasri Ramasubramanian, Harsha Jyothi, Leora Goldbloom-Helzner, Brandon M. Light, Priyadarsini Kumar, Randy P. Carney, Diana L. Farmer, Aijun Wang
Summary: This study validates lipid rafts as a functional biomaterial for synthesizing biomimetic nanovesicles and shows their potential in promoting neurogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Miquel Antich-Rossello, Maria Antonia Forteza-Genestra, Marta Monjo, Joana M. Ramis
Summary: Platelet-derived EVs (pEVs) show great potential as a new therapeutic approach in wound healing and regenerative medicine. Studies have demonstrated that pEVs not only participate in healing responses but also induce cellular differentiation, promoting musculoskeletal or neural regeneration. However, there is heterogeneity in the obtention and characterization of pEVs, requiring further research to enhance their translation into feasible therapeutic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Lei Cao, Tian Tian, Yuanbo Huang, Shiqin Tao, Xiaohong Zhu, Mifang Yang, Jing Gu, Guangdong Feng, Yinni Ma, Rushan Xia, Wenrong Xu, Lei Wang
Summary: The study found that ReN-NV promotes DPC proliferation and accelerates HF cycling transition in a mouse model. This acceleration is achieved through the activation of the Wnt/beta-catenin signaling pathway.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Zhiwei Liao, Shuai Li, Saideng Lu, Hui Liu, Gaocai Li, Liang Ma, Rongjin Luo, Wencan Ke, Bingjin Wang, Qian Xiang, Yu Song, Xiaobo Feng, Yukun Zhang, Xinghuo Wu, Wenbin Hua, Cao Yang
Summary: The study demonstrates that metformin enhances the release of EVs and changes their protein profile, improving the quality and quantity of MSCs-derived EVs through an autophagy-related pathway. EVs derived from metformin-treated MSCs ameliorate the senescence of intervertebral disc cells, indicating great potential for intervertebral disc regeneration.
Review
Chemistry, Multidisciplinary
Kosar Malekpour, Ali Hazrati, Sara Soudi, Seyed Mahmoud Hashemi
Summary: Mesenchymal stromal/stem cells (MSCs) exert their therapeutic effects through various mechanisms, including differentiation, production of growth factors, immunomodulatory factors, and extracellular vesicles. Recent studies have also highlighted the therapeutic potential of MSCs through mitochondrial transfer, with different methods of transfer being investigated. Pathological conditions can cause mitochondrial damage in cells, leading to decreased ATP production and cell death. Restoring mitochondrial content, function, and homeostasis through MSCs mitochondrial transfer can have significant effects on cell attributes and behavior, crucial for therapeutic purposes.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Medicine, Research & Experimental
Chaoxiang Chen, Jialin Wang, Mengdi Sun, Jian Li, Hui-Min David Wang
Summary: Phytochemicals are widely used for therapeutic purposes, and cell-derived nanovesicles show great potential in drug delivery due to their higher stability and superior biocompatibility, which can better encapsulate bioactive molecules.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Yuan Ding, Yanjie Li, Zhongquan Sun, Xin Han, Yining Chen, Yao Ge, Zhengwei Mao, Weilin Wang
Summary: Human regenerative ability is limited, leading to the use of cell-derived biomaterials for tissue repair. Stem cells and other multipotent cells have been used for therapy, but the paracrine effect rather than cell differentiation is often the key driver of tissue repair. Nonliving cell-derived techniques like extracellular vesicles and cell membrane-based therapy are important for fulfilling the demand for tissue repair, offering safer and more controllable options.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Aixue Li, Dan Li, Yongwei Gu, Rongmei Liu, Xiaomeng Tang, Yunan Zhao, Fu Qi, Jifu Wei, Jiyong Liu
Summary: Extracellular vesicles (EVs) are actively secreted phospholipid bilayer vesicles containing various functional nucleic acids, proteins, and lipids. Plant-derived nanovesicles (PNVs) have advantages of simplicity, safety, eco-friendliness, low cost, and low toxicity, making them potential therapeutics and drug delivery carriers. This review provides a comprehensive analysis of PNVs, including their biogenesis, isolation methods, physical characterization, components, therapeutic potential, and possible challenges in future development.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Cell & Tissue Engineering
A. Ivica, M. Zehnder, F. E. Weber
Summary: Regenerative endodontic procedures involve two tissue engineering approaches, one using transplanted mesenchymal stem cells and the other relying on bioactive molecules. A potential third approach involves the application of MSC-derived exosomes for pulp regeneration. Future research should focus on investigating the therapeutic use of these exosomes in in-vivo and clinical studies.
EUROPEAN CELLS & MATERIALS
(2021)
Review
Immunology
Marco Quaglia, Guido Merlotti, Andrea Colombatto, Stefania Bruno, Alessandra Stasi, Rossana Franzin, Giuseppe Castellano, Elena Grossini, Vito Fanelli, Vincenzo Cantaluppi
Summary: Acute kidney injury is a common and serious complication of hospitalized patients. Current treatment options are limited, but stem cell-derived extracellular vesicles show promise in treating acute kidney injury by directly interfering with pathogenic mechanisms and promoting cell protection, regeneration, and immunomodulation. Additionally, extracellular vesicles may have an impact on the transition from acute kidney injury to chronic kidney disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Marcel Franco Mougenot, Vanessa Sousa Pereira, Ana Leticia Rodrigues Costa, Marcelo Lancellotti, Marimelia Aparecida Porcionatto, Juliano Coelho da Silveira, Lucimara Gaziola de la Torre
Summary: Biomimetic nanovesicles have emerged as a promising strategy for drug and gene delivery systems, combining the advantages of biological vesicles with the controllability of synthetic systems. This article focuses on tracking the research progress in biomimetic nanovesicles applied in drug and gene delivery systems, as well as vaccines applications, describing the different sources of natural vesicles, the technical perspectives on obtaining them, and the possibility of their hybridization with synthetic liposomes.
Review
Biochemistry & Molecular Biology
Ciarra Almeria, Sebastian Kress, Viktoria Weber, Dominik Egger, Cornelia Kasper
Summary: Extracellular vesicles (EVs) are cell-derived membrane structures that play a major role in physiological and pathological processes by delivering biomolecules to target cells. Many effects previously attributed to cell-based therapies are actually mediated by EVs, suggesting the use of purified EVs instead of living cells for therapy. This review focuses on the heterogeneity of EVs derived from mesenchymal stem/stromal cells (MSC) and summarizes the upstream parameters that critically affect their therapeutic properties and biological functions.
CELL AND BIOSCIENCE
(2022)
Review
Cardiac & Cardiovascular Systems
Sean M. Davidson, Chantal M. Boulanger, Elena Aikawa, Lina Badimon, Lucio Barile, Christoph J. Binder, Alain Brisson, Edit Buzas, Costanza Emanueli, Felix Jansen, Miroslava Katsur, Romaric Lacroix, Sai Kiang Lim, Nigel Mackman, Manuel Mayr, Philippe Menasche, Rienk Nieuwland, Susmita Sahoo, Kaloyan Takov, Thomas Thum, Pieter Vader, Marca H. M. Wauben, Kenneth Witwer, Joost P. G. Sluijter
Summary: This article provides an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. It discusses methods for determining the protein, RNA, and lipid content of EVs. The guidance provided in this document is important for investigating the role of EVs in cardiovascular diseases and highlights key methodological issues to consider.
CARDIOVASCULAR RESEARCH
(2023)
Review
Cell Biology
Juntao Fang, Alain de Bruin, Andreas Villunger, Raymond Schiffelers, Zhiyong Lei, Joost P. G. Sluijter
Summary: Polyploid cells, containing more than one set of chromosome pairs, are common in nature. They provide cells with several advantages, including increased cell size, organ growth, tissue homeostasis, and enhanced tolerance to genomic stress and apoptotic signals. This article focuses on the reasons for polyploidy, the stress responses and molecular signals involved, as well as its crucial roles in cell growth and tissue regeneration in the heart, liver, and other tissues.
Article
Biology
Patricia van den Hoogen, Manon M. H. Huibers, Floor W. van den Dolder, Roel de Weger, Erica Siera-de Koning, Marish I. F. Oerlemans, Nicolaas de Jonge, Linda W. van Laake, Pieter A. Doevendans, Joost. P. G. Sluijter, Aryan Vink, Saskia C. A. de Jager
Summary: Heart transplantation is the gold standard for patients with end-stage heart failure, but immune responses to the transplanted heart can lead to complications and graft failure. Elevated levels of antibodies prior to transplantation are associated with poor outcomes after transplantation. Specifically, higher levels of IgG1 and IgG2 antibodies are associated with increased rejection of the transplanted heart and remain elevated after transplantation. This suggests that heart transplant patients with a more active antibody-mediated immune response might benefit from expanded immunosuppressive therapy.
Article
Chemistry, Multidisciplinary
Nazma F. Ilahibaks, Arif I. Ardisasmita, Songpu Xie, Anders Gunnarsson, Joseph Brealey, Pieter Vader, Olivier G. de Jong, Saskia de Jager, Niek Dekker, Ben Peacock, Raymond M. Schiffelers, Joost P. G. Sluijter, Zhiyong Lei
Summary: Extracellular vesicles (EVs) are being explored as biocompatible drug delivery vehicles for their ability to deliver bioactive cargo to recipient cells. However, effective methods for loading and unloading proteins inside EVs remain a challenge. In this study, we present the Technology Of Protein delivery through Extracellular Vesicles (TOP-EVs) as an efficient tool for intracellular protein delivery mediated by EVs. Our results demonstrate the versatility and potential of TOP-EVs for protein-based therapeutics in vitro and in vivo.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Madison Jade Ainsworth, Nino Chirico, Mylene de Ruijter, Andrei Hrynevich, Inge Dokter, Joost P. G. Sluijter, Jos Malda, Alain van Mil, Miguel Castilho
Summary: In order to develop thick engineered cardiac tissue construct with a pre-vascularization pathway, a combination of extrusion-based bioprinting and melt electrowriting techniques was investigated. Gelatin methacryloyl (GelMA) was used as a base hydrogel and collagen, Matrigel, and fibrinogen were utilized as interpenetrating polymers to support myocardial functionality. An anatomically inspired vascular pathway was implemented in a dual extrusion-based bioprinting setup. The optimized bioinks effectively filled the microfiber mesh and maintained cell viability, and distinct cell populations were visualized in their respective patterns up to D14.
Article
Pharmacology & Pharmacy
Laura E. Swart, Marcel H. A. M. Fens, Anita van Oort, Piotr Waranecki, L. Daniel Mata Casimiro, David Tuk, Martijn Hendriksen, Luca van den Brink, Elizabeth Schweighart, Cor Seinen, Ryan Nelson, Anja Krippner-Heidenreich, Tom O'Toole, Raymond M. Schiffelers, Sander Kooijmans, Olaf Heidenreich
Summary: Lipid nanoparticles (LNPs) are rapidly evolving as promising delivery systems for oligonucleotides, including siRNAs. In this study, LNPs were specifically targeted to hematopoietic progenitor cells in the bone marrow using a modified tripeptide ligand. Functionalized LNPs showed improved uptake and delivery of siRNA in patient-derived leukemia cells, as well as increased accumulation and retention in the bone marrow. These findings suggest the potential of LNPs for targeted therapy in leukemia and other hematological disorders.
Article
Biology
Maria Laura Tognoli, Julia Dancourt, Emeline Bonsergent, Roberta Palmulli, Olivier G. de Jong, Guillaume Van Niel, Eric Rubinstein, Pieter Vader, Gregory Lavieu
Summary: Extracellular vesicles (EVs) play a role in intercellular communication by transferring substances from donor to acceptor cells. The process of EV content delivery within acceptor cells and the involvement of CD63 and CD9 tetraspanins in this process have been unclear. This study used different cell models to investigate the potential role of CD63 and CD9 in EV uptake and cargo delivery, and found that neither CD63 nor CD9 is required for these functions.
COMMUNICATIONS BIOLOGY
(2023)
Review
Developmental Biology
Renee G. C. Maas, Floor W. van den Dolder, Qianliang Yuan, Jolanda van der Velden, Sean M. Wu, Joost P. G. Sluijter, Jan W. Buikema
Summary: Developmental research has revealed the key roles of Wnt and Hippo signaling in heart growth, but there are currently no clinically relevant therapies for cardiomyopathies. Understanding the decline in proliferation in adult hearts and finding new strategies for cardiac regeneration remain important. Multiple signaling pathways that regulate cardiomyocyte proliferation have been identified and upregulated in injured hearts. This review highlights the interaction of signaling pathways in heart development and their translation into current technologies for cardiomyocyte production.
Article
Chemistry, Multidisciplinary
Emile F. van Vliet, Maarten J. Knol, Raymond M. Schiffelers, Massimiliano Caiazzo, Marcel H. A. M. Fens
Summary: Parkinson's disease is characterized by degeneration of dopaminergic neurons, causing dopamine deficiency and motor symptoms. Current treatment using L-DOPA has limitations and side effects. Encapsulation of L-DOPA into nanoparticles can protect it and improve delivery to the brain, reducing required dosages and side effects. Alternative delivery methods, such as intravenous administration and transdermal delivery, enhance therapeutic effects. This review discusses recent advances and future perspectives on NP-mediated L-DOPA delivery to the brain.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
C. M. van Kammen, S. J. van Woudenberg, R. Schiffelers, F. Terstappen, A. T. Lely
Summary: Preeclampsia and fetal growth restriction are pregnancy complications that can be treated with nanomedicines, which regulate drug interaction with the placenta to enhance treatment efficacy and minimize fetal exposure. Liposomes and polymeric drug delivery systems show promising results in preventing trans-placental passage of nanomedicines, while quantum dots and silicon nanoparticles have limited research in placental insufficiency syndromes. Animal studies demonstrate positive effects on maternal and fetal health, but results are influenced by various factors. Further research is needed to understand the pathophysiology of these complex diseases and ensure the safety and efficacy of nanomedicines before clinical implementation.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Tom C. L. Bracco Gartner, Ye Wang, Laurynas Leiteris, Iris van Adrichem, Judith Marsman, Marie Jose Goumans, Carlijn V. C. Bouten, Joost P. G. Sluijter, Jaap M. J. den Toonder, Willem J. L. Suyker, Jesper Hjortnaes
Summary: Cardiac fibroblasts in the ever-beating human heart remain quiescent due to the antifibrotic effect of cyclic strain conditions revealed by a novel platform for studying cardiac fibrosis-on-a-chip. This study provides insights into the mechanosensitive pathways and genes involved in the fibrogenic process, which can contribute to the development of new therapies against cardiac fibrosis.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ye Zeng, Mariona Estape Senti, M. Clara I. Labonia, Panagiota Papadopoulou, Maike A. D. Brans, Inge Dokter, Marcel H. Fens, Alain van Mil, Joost P. G. Sluijter, Raymond M. Schiffelers, Pieter Vader, Alexander Kros
Summary: This study demonstrates the efficient delivery of mRNA-LNPs to difficult-to-transfect cardiomyocytes using fusogenic coiled-coil peptides. Local administration via intramyocardial injection significantly enhances mRNA delivery and protein expression. These findings have the potential to advance regenerative therapies for heart failure.
Article
Cardiac & Cardiovascular Systems
Vera Loen, Agnieszka Smoczynska, Alfonso Aranda Hernandez, Coert O. S. Scheerder, Britt H. R. van der Linde, Henriette D. M. Beekman, Aina Cervera-Barea, Gerard J. J. Boink, Joost P. G. Sluijter, Marcel A. G. van der Heyden, Mathias Meine, Marc A. Vos
Summary: This study investigated the relationship between automated determination of short-term variability (STV-ARIauto) of repolarization on intracardiac electrograms and arrhythmias. The results showed that STV-ARIauto was able to predict the occurrence of arrhythmias in a porcine model of acute myocardial infarction. Additionally, the study found that high-rate pacing did not improve arrhythmic outcomes. The findings highlight the potential of using STV-ARIauto for monitoring arrhythmic risk and initiating preventive strategies.