Article
Immunology
Mateusz Maciejczyk, Milosz Nesterowicz, Anna Zalewska, Grzegorz Biedrzycki, Piotr Gerreth, Katarzyna Hojan, Karolina Gerreth
Summary: The activity and metabolic products of xanthine oxidase (XO) in saliva were found to be significantly higher in ischemic stroke patients compared to hemorrhagic stroke patients and healthy controls. Evaluating the XO-specific activity in saliva can differentiate between ischemic and hemorrhagic stroke with high sensitivity and specificity. Saliva collection is a non-invasive and convenient method, making it a potential tool for monitoring and evaluating cerebrovascular diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Chemistry, Analytical
Riccardo Goldoni, Alessandra Scolaro, Elisa Boccalari, Carolina Dolci, Antonio Scarano, Francesco Inchingolo, Paolo Ravazzani, Paola Muti, Gianluca Tartaglia
Summary: Oral cancer, a deadly malignancy, is often diagnosed at a late stage, resulting in poor survival rates. Saliva, as an alternative biofluid, shows promise for non-invasive diagnostics, but further large-scale studies are needed for clinical validation.
Review
Biochemistry & Molecular Biology
Kacper Nijakowski, Jakub Zdrojewski, Monika Nowak, Dawid Gruszczynski, Filip Knoll, Anna Surdacka
Summary: Cancer is the leading cause of death worldwide, with breast, lung, and colorectal cancer being the most common types. Salivary metabolome profiling is a novel non-invasive method in oncological diagnosis. This systematic review found that changes in salivary metabolome, particularly in amino acid and polyamine pathways, showed significant predictive values in diagnosing breast, gastrointestinal, and lung cancers.
Review
Biochemistry & Molecular Biology
Yago Gecy de Sousa Ne, Weslley Ferreira Lima, Paulo Fernando Santos Mendes, Daiane Claydes Baia-da-Silva, Leonardo Oliveira Bittencourt, Priscila Cunha Nascimento, Renata Duarte de Souza-Rodrigues, Luiz Renato Paranhos, Paulo Antonio Martins-Junior, Rafael Rodrigues Lima
Summary: This study aimed to analyze the research trends on salivary oxidative stress associated with dental caries and to perform bibliometric approaches for existing publications on this association. The findings show that India and Asia have the highest number of publications on this topic, non-stimulated total saliva is the most commonly collected sample, and total antioxidant capacity is the most frequently evaluated parameter. Cross-sectional studies are the most common study design, and articles published from 2017 to 2022 are the most frequent.
Review
Biochemistry & Molecular Biology
Raphael Charchar Campos Alves, Railson Oliveira Ferreira, Deborah Ribeiro Frazao, Yago Gecy de Souza Ne, Paulo Fernando Santos Mendes, Guido Maranon-Vasquez, Luiz Fernando Freire Royes, Nathalia Carolina Fernandes Fagundes, Lucianne Cople Maia, Rafael Rodrigues Lima
Summary: This systematic review evaluated the effects of physical exercise on oxidative stress in saliva. The findings suggest that exercise may increase oxidative stress and antioxidant activity in saliva, but there is heterogeneity and inconsistency among studies.
Article
Pharmacology & Pharmacy
Gabriela Alice Fiais, Douglas Sadrac de Biagi Ferreira, Rayara Nogueira de Freitas, Lucas Guilherme Leite da Silva, Marcelo Kawaguchi, Allice Santos Cruz Veras, Giovana Rampazzo Teixeira, Cristina Antoniali, Rita Cassia Menegati Dornelles, Ana Claudia de Melo Stevanato Nakamune, Melo Stevanato Teixeira, Walid D. Fakhouri, Antonio Hernandes Chaves-Neto
Summary: This study investigated the effects of Levetiracetam (LEV) on the parotid and submandibular glands in rats, and found that it can lead to salivary gland dysfunction and redox state imbalance.
Article
Biology
Valentina Mangolini, Alice Gualerzi, Silvia Picciolini, Francesca Roda, Angela Del Prete, Luana Forleo, Rudy Alexander Rossetto, Marzia Bedoni
Summary: Extracellular vesicles (EVs) isolated from saliva can be comprehensively characterized by Raman spectroscopy, providing valuable biomarkers for diagnosis. EVs from blood are difficult to separate from other particles and proteins, while saliva EVs can be easily analyzed. Despite the low concentration, Raman spectroscopy is capable of accurately characterizing salivary EVs. This suggests the potential of salivary EVs for diagnostic purposes, with Raman spectroscopy playing a key role.
Review
Veterinary Sciences
J. J. Ceron, M. D. Contreras-Aguilar, D. Escribano, S. Martinez-Miro, M. J. Lopez-Martinez, A. Ortin-Bustillo, L. Franco-Martinez, C. P. Rubio, A. Munoz-Prieto, A. Tvarijonaviciute, M. Lopez-Arjona, S. Martinez-Subiela, F. Tecles
Summary: The use of saliva as a biological sample has many advantages, including its application in diagnosing infectious diseases and measuring biomarkers related to stress, inflammation, immune response, and redox homeostasis. This review provides practical data on salivary analytes, along with general rules for sampling and storage, and explores the concept of sialochemistry.
BMC VETERINARY RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Katarina Jansakova, Klaudia Kyselicova, Daniela Ostatnikova, Gabriela Repiska
Summary: Saliva, as a potential alternative to other body fluids, offers a complex view on the current state of the individual with its sources of nucleic acid of both human and microbial origin, protein, and non-protein molecules. Using salivary markers may simplify ASD screening and potentially reveal the etiopathogenesis of ASD, with the advantage of easier recruitment of neurotypical counterparts for studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Pune N. Paque, Christopher Herz, Daniel B. Wiedemeier, Konstantinos Mitsakakis, Thomas Attin, Kai Bao, Georgios N. Belibasakis, John P. Hays, Joel S. Jenzer, Wendy E. Kaman, Michal Karpisek, Philipp Korner, Johannes R. Peham, Patrick R. Schmidlin, Thomas Thurnheer, Florian J. Wegehaupt, Nagihan Bostanci
Summary: This study identified four salivary biomarkers crucial for predicting caries accurately and developed an RF model for classification and prediction of caries progression. The high correlation between RF prediction and clinical classification was observed, indicating a new approach for caries identification.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Veterinary Sciences
Takanori Kooriyama, Niwako Ogata
Summary: Research on salivary biomarkers of stress responses in dogs is important for advancing studies and improving treatment methods.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Mengjiao Zhou, Minjian Yuan, Yilan Jin, Qianqian Zhou, Yuting Yu, Jianan Li, Xing Dai, Xiaohua Zheng, Haijun Yu, Weiqi Wang
Summary: Ferroptosis, characterized by iron-dependent lipid peroxidation, plays a critical role in tumor regression. This study developed a self-assembled vitamin B2-ferric nanocomplex to regulate ferroptosis. The nanocomplex effectively delivered therapeutic agents to tumor sites and cancer cells, and synergistically promoted ferroptosis through the production of reactive oxygen species. Additionally, the use of metformin further disrupted the antioxidant system and enhanced the ferroptotic effect. This novel strategy showed promising antitumor efficacy with minimal adverse effects, providing a potential treatment option for breast carcinoma.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Medicine, General & Internal
Tanya Deneva, Youri Ianakiev, Oliana Boykinova
Summary: The study aims to analyze salivary mental stress biomarkers in hospitalized patients with COVID-19 and determine their potential relationship with stress symptoms.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Justyna Dorf, Konrad Zareba, Anna Pryczynicz, Joanna Matowicka-Karna, Boguslaw Kedra, Piotr Zukowski, Anna Zalewska, Mateusz Maciejczyk
Summary: Gastric cancer is associated with redox imbalance and increased oxidative damage to proteins, lipids, and DNA. Histopathological parameters of the tumor are associated with the level of antioxidants and oxidative damage products. Determination of redox parameters may be useful in the assessment of the tumor progression.
ANNALS OF MEDICINE
(2023)
Article
Endocrinology & Metabolism
Yeon-Hee Lee, Chon Suk, Seung-Il Shin, Ji-Youn Hong
Summary: This study aimed to investigate the neuroendocrine responses based on cortisol, dehydroepiandrosterone (DHEA), cortisol/DHEA ratio, and chromogranin A levels, which reflect the activity of the hypothalamic-pituitary-adrenal axis, according to the presence or absence of psychological stress in patients with gingivitis and periodontitis compared to that in healthy controls. The results showed that salivary cortisol and chromogranin A levels increased with the severity of periodontal disease; their levels were the highest in the periodontitis group and were significantly higher in the following descending order: periodontitis, gingivitis, and healthy control groups. Periodontitis and psychological stress were significant and strong predictors of above-average cortisol levels and cortisol/DHEA ratios.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Physiology
Sara Zieba, Mateusz Maciejczyk, Anna Zalewska
Summary: Alcohol abuse and smoking have been shown to have negative effects on the oral environment, including salivary gland dysfunction, DNA damage leading to precancerous lesions, and deterioration of periodontal health.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Mateusz Maciejczyk, Anna Zalewska, Malgorzata Gryciuk, Katarzyna Hodun, Milosz Czuba, Kamila Ploszczyca, Malgorzata Charmas, Jerzy Sadowski, Marcin Baranowski
Summary: The study evaluates the effects of various acute physical exercise conditions, including oxidative stress and inflammation under hypoxic conditions. The results show that hypoxic exercise exacerbates oxidative stress, affects the antioxidant system, increases protein and lipid oxidation, and elevates plasma nitric oxide levels.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Cell Biology
Mateusz Maciejczyk, Ewa Zebrowska, Milosz Nesterowicz, Malgorzata Zendzian-Piotrowska, Anna Zalewska
Summary: The role of alpha-lipoic acid (ALA) in reducing brain manifestations of insulin resistance was determined in this study. ALA was found to scavenge oxygen free radicals and stimulate the production of reduced glutathione (GSH), the most critical brain antioxidant. The study showed that ALA improves the function of antioxidant systems in the brain, predominantly in the hypothalamus of insulin-resistant rats. ALA reduces oxidative stress, inflammation, and apoptosis in the brain, and further research is needed to understand its molecular mechanism.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Review
Biochemistry & Molecular Biology
Milosz Nesterowicz, Malgorzata Zendzian-Piotrowska, Jerzy Robert Ladny, Anna Zalewska, Mateusz Maciejczyk
Summary: Amantadine does not significantly affect protein glycoxidation, lacks antioxidant and antiglycation effects, and may even have some glycoxidant, proglycation, and prooxidant properties.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Aleksander Nobis, Daniel Zalewski, Eliza Samaryn, Mateusz Maciejczyk, Anna Zalewska, Napoleon Waszkiewicz
Summary: Depression is a prevalent psychiatric disorder that significantly impacts individuals and public health worldwide. Recent research has focused on identifying biological markers to better understand the underlying mechanisms of depression.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Nutrition & Dietetics
Elzbieta Supruniuk, Ewa Zebrowska, Mateusz Maciejczyk, Anna Zalewska, Adrian Chabowski
Summary: This study examined the effects of high-protein diets on lipid peroxidation and sphingolipid metabolism in rats. The results showed that high-protein diets significantly impacted hypothalamic metabolic pathways, leading to increased lipid peroxidation, ceramide accumulation, and changes in sphingolipid metabolism.
Article
Immunology
Julita Szulimowska, Anna Zalewska, Katarzyna Taranta-Janusz, Grazyna Marczuk-Kolada, Malgorzata Zendzian-Piotrowska, Mateusz Maciejczyk
Summary: Chronic kidney disease (CKD) is a systemic inflammatory disease that affects multiple organs, including the kidneys, cardiovascular system, and oral cavity. Children with CKD experience reduced saliva secretion (hyposalivation), leading to increased dental caries and impaired quality of life. This study evaluated the inflammatory and anti-inflammatory profile in the saliva of CKD children at different stages of renal failure. The results showed significant changes in salivary cytokines, chemokines, and growth factors in CKD children compared to healthy controls.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Medicine, General & Internal
Justyna Dorf, Konrad Zareba, Anna Pryczynicz, Joanna Matowicka-Karna, Boguslaw Kedra, Piotr Zukowski, Anna Zalewska, Mateusz Maciejczyk
Summary: Gastric cancer is associated with redox imbalance and increased oxidative damage to proteins, lipids, and DNA. Histopathological parameters of the tumor are associated with the level of antioxidants and oxidative damage products. Determination of redox parameters may be useful in the assessment of the tumor progression.
ANNALS OF MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Anna Zalewska, Bozena Antonowicz, Julita Szulimowska, Izabela Zieniewska-Siemienczuk, Barbara Lesniewska, Jan Borys, Sara Zieba, Paula Kostecka-Sochon, Malgorzata Zendzian-Piotrowska, Roberto Lo Giudice, Giusseppe Lo Giudice, Piotr Zukowski, Mateusz Maciejczyk
Summary: Although titanium and its alloys have high biocompatibility, there is a growing debate regarding the removal of titanium implants due to potential long-term adverse effects. To enhance the biocompatibility of titanium implants, increasing the thickness of the titanium dioxide passive layer is being explored. In this study, we compared the cytotoxicity, mitochondrial function, and redox balance of fibroblast mitochondria between hard-anodized (type II), standard-anodized (type III), and non-anodized titanium discs. The results showed oxidative stress in fibroblast mitochondria regardless of anodization, but type II anodization prevented changes in complex II activity, had the lowest citrate synthase inhibition, and reduced cytochrome c oxidase inhibition, potentially preventing apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Dariusz Juchnowicz, Michal Dzikowski, Joanna Rog, Napoleon Waszkiewicz, Kaja Hanna Karakula, Anna Zalewska, Mateusz Maciejczyk, Hanna Karakula-Juchnowicz
Summary: This study assessed the utility of complete blood count (CBC) in the prediction of schizophrenia diagnosis and its relationship with oxidative stress (OS). The findings showed that certain CBC parameters were altered in patients with the first episode of psychosis compared to healthy individuals, and there were various associations between OS and CBC. The study also proposed promising CBC models for distinguishing different patient groups.
Article
Medicine, General & Internal
Monika Kamianowska, Aleksandra Kamianowska, Mateusz Maciejczyk, Anna Kurowska, Bozena Blazewicz, Agnieszka Maria Rogowska, Anna Wasilewska
Summary: This study analyzed the concentrations of renal biomarkers (osteopontin, cystatin C, and NGAL) in neonates and found that prematurity may lead to increased excretion of osteopontin and cystatin C, but not NGAL. The excretion of NGAL and cystatin C may change during the first weeks of a premature neonate's life, but osteopontin does not.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Monika Kamianowska, Agnieszka Rybi-Szuminska, Aleksandra Kamianowska, Mateusz Maciejczyk, Katarzyna Solomianko, Alicja Koput, Anna Wasilewska
Summary: This study aimed to assess the urinary concentration of trefoil factor 3 (TFF3) in healthy term and stable preterm neonates. The results showed higher TFF3/cr. values in preterm neonates compared to term neonates, and male term neonates had increased urinary TFF3 excretion. These findings have important implications for the diagnosis in neonatology.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Chemistry, Medicinal
Grzegorz Biedrzycki, Blanka Wolszczak-Biedrzycka, Justyna Dorf, Daniel Michalak, Malgorzata Zendzian-Piotrowska, Anna Zalewska, Mateusz Maciejczyk
Summary: This study compares the antioxidant and antiglycation potentials of popular H2 antagonists (ranitidine, cimetidine, and famotidine) on protein glycoxidation. The results show that ranitidine is the only H2 blocker that significantly inhibits protein glycation and has antioxidant properties. It can compete with proteins for the addition of carbonyl groups.
Article
Chemistry, Medicinal
Emilia Szymanska, Joanna Potas, Mateusz Maciejczyk, Magdalena Ewa Sulewska, Malgorzata Pietruska, Anna Zalewska, Aleksandra Pietruska, Katarzyna Winnicka
Summary: This study tested different emulgel compositions for the delivery of delta-aminolevulinic acid and investigated the impact of the drug on the behavior of gelling agents. The results showed that a combination of xanthan and gellan gum enhanced the retention of the emulgels in oral mucosal tissue. In addition, the incorporation of delta-aminolevulinic acid promoted interactions with mucosal tissue. Cytotoxicity studies demonstrated that emulgels based on tragacanth and gellan/xanthan may have a protective effect on the metabolic activity of human gingival fibroblasts and keratinocytes.
Article
Dentistry, Oral Surgery & Medicine
Joanna Toczewska, Dagmara Baczynska, Anna Zalewska, Mateusz Maciejczyk, Tomasz Konopka
Summary: This study evaluated the expression of genes encoding antioxidant enzymes in the saliva and gingival tissue of patients with periodontitis. The results showed that the expression of GPX1 and TXN1 genes in saliva was significantly higher, while the expression of SOD1, GPX1, and TXN1 genes in gingival tissue was significantly lower in periodontitis patients compared to healthy individuals. Additionally, the activity of GPX1 in unstimulated saliva, SOD1 in stimulated saliva, and both antioxidant enzymes in gingival crevicular fluid were lower in periodontitis patients.
DENTAL AND MEDICAL PROBLEMS
(2023)