Article
Biotechnology & Applied Microbiology
Jin Li, Peng Wang, Yang Zhou, Hong Liang, Yang Lu, Kuan Luan
Summary: An automatic classification method based on deep transfer learning was proposed, which resolved the issue of repetition of low-level features and improved the accuracy of classification of colorectal cancer lymph node metastasis.
Article
Radiology, Nuclear Medicine & Medical Imaging
Suyun Li, Zhenhui Li, Li Wang, Mimi Wu, Xiaobo Chen, Chutong He, Yao Xu, Mengyi Dong, Yanting Liang, Xin Chen, Zaiyi Liu
Summary: This study evaluated the feasibility of using clinicopathological characteristics and CT morphological features to predict lymph node metastasis in patients with T1 colorectal cancer. The presence of necrosis, irregular outer border, and heterogeneity enhancement, as well as tumor location, were found to be independent risk factors for predicting lymph node metastasis. These findings can assist doctors in making decisions about additional surgery after endoscopic resection.
EUROPEAN RADIOLOGY
(2023)
Article
Oncology
Xi Wang, Guangyu Gao, Zhengrong Chen, Zhihao Chen, Mingxiao Han, Xiaolu Xie, Qiyuan Jin, Hong Du, Zhifei Cao, Haifang Zhang
Summary: This study identified potential biomarkers in miRNAs and genes for colorectal cancer (CRC) by systematic bioinformatic analysis and experimental validation. A novel miRNA-mRNA network was established for CRC lymph node metastasis, which may serve as a potential biomarker in the development of lymph node metastatic CRC.
CANCER CELL INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Xiaodong Wang, Ying Chen, Yunshu Gao, Huiqing Zhang, Zehui Guan, Zhou Dong, Yuxuan Zheng, Jiarui Jiang, Haoqing Yang, Liming Wang, Xianming Huang, Lirong Ai, Wenlong Yu, Hongwei Li, Changsheng Dong, Zhou Zhou, Xiyang Liu, Guanzhen Yu
Summary: The study proposes a deep-learning framework to analyze lymph node whole-slide images, identifying lymph nodes and tumor regions, and revealing the importance of the tumor-area-to-MLN-area ratio (T/MLN) in predicting the prognosis of gastric cancer patients.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Yingdian Yu, Wenyuan Xue, Zefeng Liu, Shang Chen, Jun Wang, Quanzhou Peng, Linhao Xu, Xin Liu, Chunhui Cui, Jian-Bing Fan
Summary: The novel methylation marker LBX2 could be used as a simple, cost-effective, and reliable diagnostic method for lymph node metastasis of colorectal cancer, outperforming conventional diagnostic methods.
FRONTIERS IN ONCOLOGY
(2022)
Review
Medicine, Research & Experimental
Jiajing Sun, Sikai Wu, Zixian Jin, Sijia Ren, William C. Cho, Chengchu Zhu, Jianfei Shen
Summary: Lung cancer has high morbidity and mortality rates, with tumor metastasis being a significant risk factor for death. Even after complete removal of a tumor at an early stage, there is still a risk of recurrence. Lymph node micrometastasis has been found to be associated with poor prognosis, but predicting prognosis and choosing the appropriate surgical and postoperative adjuvant chemotherapy options remain challenging for clinicians.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Oncology
Sinan Omeroglu, Selcuk Gulmez, Orhan Uzun, Aziz Serkan Senger, Ozgur Bostanci, Onur Guven, Erdal Polat, Mustafa Duman
Summary: In this retrospective study, it was found that a metastatic lymph node (MLN) diameter greater than 1.05 cm may indicate a poorer prognosis and lower survival rate in patients with stage III colorectal cancer (CRC). Therefore, the MLN diameter may be an important indicator for predicting the prognosis of CRC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Urology & Nephrology
Kai Yao, Yue Chen, Yunlin Ye, Zhiming Wu, Dong Chen, Hui Han, Zaishang Li, Zhuowei Liu, Yanjun Wang, Zike Qin, Yonghong Li, Zhiyong Li, Fangjian Zhou
Summary: The study provides a detailed map of pelvic lymph node metastasis in patients with penile carcinoma, showing that the external iliac and obturator packages are most commonly involved. Pelvic lymph node metastasis is associated with advanced T-stage. Some patients have crossover metastasis.
JOURNAL OF UROLOGY
(2021)
Article
Oncology
Jiaojiao Zhao, Han Wang, Yin Zhang, Rui Wang, Qin Liu, Jie Li, Xue Li, Hanyu Huang, Jie Zhang, Zhaoping Zeng, Jun Zhang, Zhang Yi, Fanxin Zeng
Summary: This study developed a deep learning model and a combined model for predicting lymph node metastasis in colorectal cancer (CRC) and analyzed the genes and pathways related to the models. These models showed good performance in predicting lymph node metastasis and have the potential to be utilized for personalized treatment prediction in CRC patients.
RADIOTHERAPY AND ONCOLOGY
(2022)
Article
Oncology
Kenta Kasahara, Kenji Katsumata, Akira Saito, Tetsuo Ishizaki, Masanobu Enomoto, Junichi Mazaki, Tomoya Tago, Yuichi Nagakawa, Jun Matsubayashi, Toshitaka Nagao, Hiroshi Hirano, Masahiko Kuroda, Akihiko Tsuchida
Summary: This study used artificial intelligence to analyze biopsy specimens of T1 CRC and created a risk model for lymph node metastasis. The results showed that the model could improve the accuracy of preoperative diagnosis.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Oncology
Xueling Wang, Haoran Li, Xiaotian Chang, Zibin Tian
Summary: This study evaluated the ability of serum monosaccharides to predict lymph node metastasis and prognosis in colorectal cancer patients. The results showed that high levels of serum mannose and galactose were associated with lymph node metastasis, and mannose performed better than carcinoembryonic antigen in predicting lymph node metastasis. Histologic grade, lymphovascular invasion, CEA, and mannose levels were identified as independent risk factors for lymph node metastasis. Additionally, high serum mannose was associated with poor prognosis in colorectal cancer patients.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, General & Internal
Lei Chen, Funing Yang, Zhaoyan Qi, Jiandong Tai
Summary: Tumor budding (TB), a powerful predictor of colorectal cancer (CRC), was studied to develop a prediction model for lymph node metastasis (LNM) and local recurrence in pT1 CRC patients. Independent predictors for LNM and recurrence were identified and used to construct predictive nomograms for treatment decision-making. The models showed potential clinical application value and postoperative chemotherapy was associated with better outcomes in high-risk patients.
FRONTIERS IN MEDICINE
(2022)
Article
Surgery
Chao-Tao Tang, Jun Li, Peng Wang, You-Xiang Chen, Chun-Yan Zeng
Summary: A noninvasive nomogram was established to predict lymph node metastasis after endoscopic surgery for superficial colorectal cancer using common clinicopathologic factors. The nomogram showed superior performance compared to traditional CT imaging in risk stratification of lymph node metastasis.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Oncology
Soo Hyun Lee, Amaya Pankaj, Azfar Neyaz, Yuho Ono, Steffen Rickelt, Cristina Ferrone, David Ting, Deepa T. Patil, Omer Yilmaz, David Berger, Vikram Deshpande, Osman Yilmaz
Summary: This study found that a higher lymph node yield is associated with improved survival in patients with colorectal cancer. The high lymph node yield group was characterized by younger patients, larger tumors, right-sided location, and tumors with mismatch repair deficiency. The tumor microenvironment in the high lymph node yield group showed higher infiltration of CD8+ cells and expression of B2MG.
BRITISH JOURNAL OF CANCER
(2023)
Article
Surgery
Taketo Sasaki, Kohei Shigeta, Shimpei Matsui, Ryo Seishima, Koji Okabayashi, Yuko Kitagawa
Summary: The location of lymph node metastasis (LNM) in colorectal cancer has a significant impact on recurrence risk. LNM adjacent to the tumor, horizontal or vertical spread, and both horizontal and vertical spread have different risks for recurrence. Considering the location of LNM can improve the accurate prediction of colorectal cancer prognosis.
ANZ JOURNAL OF SURGERY
(2023)
Review
Biochemistry & Molecular Biology
Giovanna Maria Stanfoca Casagrande, Marcela de Oliveira Silva, Rui Manuel Reis, Leticia Ferro Leal
Summary: Lung cancer, the most deadly cancer globally, can be diagnosed and molecularly stratified using tissue biopsy. Liquid biopsy, a minimally invasive approach, allows the detection of biomarkers from body fluids for diagnosis, prognosis, and treatment response prediction. Liquid biopsy options for managing lung cancer have emerged, including the detection of EGFR p.(Tyr790Met) mutation in plasma samples to predict response and monitor TKI-treated patients. Efforts are ongoing to improve the detection of clinically relevant biomarkers for lung cancer using more sensitive technologies. Liquid biopsy can also significantly reduce turnaround time for laboratory reports, accelerating treatment initiation and improving overall survival of lung cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Julieta Afonso, Andreia Barbosa, Paula Roberta Aguiar Pastrez, Murilo Bonatelli, Ricardo Filipe Alves da Costa, Celine Pinheiro, Adhemar Longatto-Filho, Fatima Baltazar
Summary: Glycolytic activity is associated with poor clinicopathological profile in esophageal cancer patients. MCT4 and CAIX positivity independently predict a worse prognosis. Further studies are needed to assess the predictive value of these biomarkers in treatment response and explore the impact of MCT1/4 targeting and HKII expression and function.
Article
Oncology
Julieta Afonso, Celine Goncalves, Marta Costa, Debora Ferreira, Lucio Santos, Adhemar Longatto-Filho, Fatima Baltazar
Summary: Glycolysis-related proteins are associated with bladder cancer aggressiveness and poor prognosis. The HK2 inhibitor 2-deoxy-D-glucose can inhibit glycolysis, reduce bladder cancer cell invasion, and increase sensitivity to cisplatin.
Article
Biochemistry & Molecular Biology
Lorena R. Sousa, Ana Gabriela S. Oliveira, Antonio Arantes, Joao Gabriel M. Junqueira, Gerso P. Alexandre, Vanessa G. P. Severino, Rui Manuel Reis, Bonglee Kim, Rosy I. M. A. Ribeiro
Summary: This study investigated the effects of four fractions of hexane and ethyl acetate extract of Annona coriacea Mart. against GBM cell lines. The fractions showed selective cytotoxicity to GBM cells compared to TMZ, and also inhibited cell migration, MMP-2 activity, and induced necroptosis-like morphological changes. These findings suggest that the fractions of A. coriacea Mart. should be further studied for potential GBM treatment.
Article
Oncology
Rhafaela Lima Causin, Andre Van Helvoort Lengert, Izabela Natalia Faria Gomes, Ana Julia Aguiar De Freitas, Marcela Nunes Rosa, Ricardo Dos Reis, Rui Manuel Reis, Marcia Maria Chiquitelli Marques
Summary: miR-130a-3p is overexpressed in cervical cancer cells and plays a significant role in the development of cervical cancer. Inhibiting miR-130a-3p can significantly reduce the proliferation, migration, and invasion of cervical cancer cells. miR-130a-3p may regulate the biological functions of cervical cancer cells by directly targeting the DLL1 gene. Therefore, miR-130a-3p may serve as a biomarker for determining cervical cancer progression.
Article
Cell Biology
Rodrigo de Oliveira Cavagna, Icaro Alves Pinto, Flavia Escremim de Paula, Gustavo Noriz Berardinelli, Debora Sant'Anna, Iara Santana, Vinicius Duval Da Silva, Eduardo Caetano Albino da Silva, Jose Elias Miziara, Josiane Mourao, Augusto Antoniazzi, Alexandre Jacinto, Pedro De Marchi, Miguel Angel Molina, Leticia Ferro Leal, Rui Manuel Reis
Summary: TP53 mutations are common in Brazilian lung adenocarcinomas, especially in lung adenocarcinomas with a prevalence of 60.4%. Disruptive and truncating mutations in TP53 gene are associated with younger age, African ancestry, and shorter overall survival.
Article
Oncology
Paula Roberta Aguiar Pastrez, Ana Margarida Barbosa, Vania Sammartino Mariano, Rhafaela Lima Causin, Antonio Gil Castro, Egidio Torrado, Adhemar Longatto-Filho
Summary: Esophageal squamous cell carcinoma (ESCC) is a rapidly progressing cancer with high mortality rates. In this study, we investigated the levels of inflammatory cytokines in ESCC patients and healthy individuals. We found increased levels of IL-1 beta, IL-6, IL-8, and IL-10 in ESCC patients. Among these cytokines, IL-8 was identified as an independent prognostic factor for ESCC. Additionally, patients with low IL-6, IL-8, TNM I/II, or those who underwent surgery had a higher overall survival rate. Our findings suggest IL-8 as a potential biomarker for ESCC.
Article
Biotechnology & Applied Microbiology
Ana Isabel Mendes, Alexandra Gabriel Fraga, Maria Joao Peixoto, Ivo Aroso, Adhemar Longatto-Filho, Alexandra Pinto Marques, Jorge Pedrosa
Summary: Diabetic foot infection (DFI) is a significant cause of illness and death. Antibiotics are an essential treatment for DFI, but their effectiveness can be reduced due to bacterial biofilm formation and associated pathophysiology. Therefore, improved antibiotic therapies are needed to manage DFI more safely and effectively.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Health Care Sciences & Services
Fabiana L. Vazquez, Henrique C. S. Silveira, Ubirani B. Otero, Thais T. Hosokawa, Jose Humberto T. G. Fregnani, Adhemar Longatto-Filho, Rui M. Reis
Summary: This study aimed to develop an online questionnaire using the REDCap data management platform based on the Work-Related Cancer Surveillance Guidelines reported by the Brazilian National Cancer Institute to obtain a history of occupational exposure in the workplace. The questionnaire was simple and easy to use, allowing for the collection of information on the occupational history of cancer patients and enabling compulsory notification of work-related cancer. The questionnaire included questions about the use of and exposure to carcinogenic factors at work and due to smoking. Additionally, a website was created as a result of this study.
Article
Biotechnology & Applied Microbiology
Mariana de Oliveira Klein, Luiza Flavia Veiga Francisco, Izabela Natalia Faria Gomes, Sergio Serrano, Rui M. Reis, Henrique C. S. Silveira
Summary: This study analyzed the cytotoxicity and genotoxicity of anticancer drugs and their metabolites in aquatic ecosystems. The results showed that these compounds induced cytotoxic and genotoxic effects in cells at concentrations found in the environment. These findings provide valuable insights for the development of public policies to minimize potential environmental contamination.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2023)
Article
Plant Sciences
Ana Gabriela Silva Oliveira, Marina Andrade Rocha, Lucas Santos de Azevedo, Aline Thaynara de Moura Coelho, Rafael Cesar Russo Chagas, Helio Batista Santos, Ralph Gruppi Thome, Peter Samuel, Evelyn Wolfram, Bonglee Kim, Rui Manuel Reis, Rosy Iara Maciel Azambuja Ribeiro
Summary: This study investigated the constituents and antitumor potential of Tapirira guianensis in glioblastoma. The results showed that T. guianensis extracts have potential antitumor effects and can reduce tumor size and induce blood vessel shrinkage.
Article
Cardiac & Cardiovascular Systems
Joana Ferreira, Julieta Afonso, Alexandre Lima Carneiro, Isabel Vila, Cristina Cunha, Susana Roque, Cristina Silva, Amilcar Mesquita, Jorge Cotter, Margarida Correia-Neves, Armando Mansilha, Adhemar Longatto-Filho, Pedro Cunha
Summary: This study compared the characteristics of visceral, subcutaneous, and peri-aortic adipose tissue using CT scans and correlated them with cardiovascular risk factors. Peri-aortic adipose tissue had the highest density, while smokers/ex-smokers had lower density of subcutaneous adipose tissue. Sex was found to be a predictor of subcutaneous adipose tissue area, while hypertension was associated with higher visceral adipose tissue area.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Oncology
Florinda A. Santos, Rui Manuel Reis, Lucas C. Barroti, Allan A. L. Pereira, Marcus M. Matsushita, Ana Carolina de Carvalho, Jose Guilherme Datorre, Gustavo N. Berardinelli, Raphael L. C. Araujo
Summary: This retrospective cohort study evaluated the overall survival (OS), RAS, BRAF, and MSI frequencies in refractory mCRC patients treated with cetuximab. The study identified BRAF V600E, KRAS, and NRAS gene mutations, as well as MSI status, in pre-treated patients. The study found that BRAF, NRAS mutations, and MSI-High status were associated with shorter OS, while tumour laterality remained an independent prognostic factor.
JOURNAL OF GASTROINTESTINAL CANCER
(2023)
Review
Medicine, General & Internal
D. E. Zantut-Wittmann, A. C. Laus, D. A. Moreno, I. S. Barreto, C. A. Moma, F. F. R. Maia, E. C. S. C. Etchebehere, L. V. M. Assumpcao, R. M. Reis
Summary: BRAF and TERT oncogenes hotspot mutations are associated with a more aggressive outcome in thyroid carcinomas. TERT promoter (pTERT) mutations (C228T and C250T) are related to cancer growth and reduced overall-and disease-free survivals in TC. This report describes a patient with a poorly differentiated thyroid carcinoma (PDTC) who developed a large volume of metastases in a short period, and molecular analysis revealed two pTERT mutations (C228T and C250T), suggesting a relationship between pTERT mutations and aggressive course in PDTC.
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
(2023)
Article
Oncology
Pedro De Marchi, Leticia Ferro Leal, Luciane Sussuchi da Silva, Rodrigo de Oliveira Cavagna, Flavio Augusto Ferreira da Silva, Vinicius Duval da Silva, Eduardo C. A. da Silva, Augusto O. Saito, Vladmir C. Cordeiro de Lima, Rui Manuel Reis
Summary: The TIS and IFN-gamma signatures are predictive biomarkers for identifying NSCLC patients who could potentially benefit from immune checkpoint inhibitor therapies.
TRANSLATIONAL ONCOLOGY
(2024)
