Article
Biochemistry & Molecular Biology
Chunru Wang, Junyao Li, Xiangsheng Han, Shuai Liu, Xintao Gao, Chuanlong Guo, Xiaochen Wu
Summary: Silk sericin (SS) as a drug nanocarrier shows great potential in therapeutic and pharmaceutical applications due to its excellent biocompatibility and bioactivity. The SS-stabilized-drug composites, such as SS/PAC, can disperse uniformly in aqueous solution with high drug loading amount, exhibiting high antioxidant efficiency and excellent biocompatibility, which can significantly alleviate symptoms of ulcerative colitis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Peiyao Wu, Siyu Yao, Xing Wang, Long Yang, Siling Wang, Wenbing Dai, Hua Zhang, Bing He, Xueqing Wang, Shujun Wang, Qiang Zhang
Summary: This study developed orally administrated IND nanoparticles (IND-NPs) for the treatment of ulcerative colitis (UC) and investigated their functions and mechanisms in cellular and animal inflammatory models. The IND-NPs were found to improve UC-associated symptoms, inhibit inflammatory response, and preserve intestinal barrier integrity through regulating intercellular junctions and restoring mitochondrial dysfunction, showing a promising potential in UC treatment.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Chunru Wang, Yingjie Xiang, Wenjie Ma, Chuanlong Guo, Xiaochen Wu
Summary: Fisetin is stabilized using silk sericin to create a composite (SS/FT), which effectively alleviates the symptoms of ulcerative colitis.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Biomedical
Sheng Xu, Qianqian Yang, Riyuan Wang, Chi Tian, Yanting Ji, Huanhuan Tan, Ping Zhao, David L. Kaplan, Feng Wang, Qingyou Xia
Summary: This study reports the development of a silk sericin nanosphere system for the delivery of lactoferrin to treat ulcerative colitis. The system demonstrated stable morphology, specific targeting to colonic sites, and efficient cell uptake. Oral administration of the system effectively relieved symptoms of ulcerative colitis in mice and exhibited comparable therapeutic effect to high-dose lactoferrin treatment. These findings indicate the promising potential of the silk sericin nanosphere system for colitis treatment.
ACTA BIOMATERIALIA
(2022)
Article
Gastroenterology & Hepatology
Markus F. Neurath, Michael Vieth
Summary: Mucosal healing is an important prognostic factor in the management of patients with inflammatory bowel disease. It can predict clinical remission and resection-free survival. There are two levels of mucosal healing: endoscopic healing and histological healing. Drug therapies and new techniques can have an impact on mucosal healing, but cannot provide a definitive cure.
Article
Biochemistry & Molecular Biology
Keiko Maeda, Masanao Nakamura, Takeshi Yamamura, Tsunaki Sawada, Eri Ishikawa, Akina Oishi, Shuji Ikegami, Naomi Kakushima, Kazuhiro Furukawa, Tadashi Iida, Yasuyuki Mizutani, Takuya Ishikawa, Eizaburo Ohno, Takashi Honda, Masatoshi Ishigami, Hiroki Kawashima
Summary: This study analyzed the usefulness of serum gelsolin as a serological biomarker for clinical and endoscopic activities in ulcerative colitis. The findings indicate that serum gelsolin level correlates with clinical and endoscopic activities in ulcerative colitis, has higher sensitivity and specificity than C-reactive protein, and can detect mucosal healing, suggesting its potential as a biomarker for ulcerative colitis.
Article
Biochemistry & Molecular Biology
Leonie Wittner, Lukas Wagener, Jakob J. Wiese, Iris Stolzer, Susanne M. Krug, Elisabeth Naschberger, Rene Jackstadt, Rudi Beyaert, Raja Atreya, Anja A. Kuehl, Gregor Sturm, Miguel Gonzalez-Acera, Jay V. Patankar, Christoph Becker, Britta Siegmund, Zlatko Trajanoski, Beate Winner, Markus F. Neurath, Michael Schumann, Claudia Guenther
Summary: The paracaspase MALT1 plays a crucial role in mucosal inflammation. It is highly expressed in colonic epithelial cells of UC patients and experimental colitis. Mechanistically, MALT1 protease function inhibits ferroptosis and contributes to NF-kappa B signaling and STAT3 signaling, which are involved in inflammation and tissue healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Ya Ma, Lian Duan, Jianfeng Sun, Shuangquan Gou, Fengyuan Chen, Yuqi Liang, Fangyin Dai, Bo Xiao
Summary: This study developed engineered Antheraea pernyi silk fibroin nanoparticles loaded with an anti-inflammatory drug, resveratrol, to target colonic epithelial cells and macrophages for effective ulcerative colitis (UC) treatment. The nanoparticles showed lysosomal escape capacity, pH and reactive oxygen species responsiveness, and targeted delivery to the cytoplasm for on-demand drug release. Oral treatment with these nanoparticles relieved UC symptoms by restoring damaged colonic epithelial barriers, reducing inflammation, and rebalancing intestinal microbiota.
Article
Biotechnology & Applied Microbiology
Cui Liu, Xiangji Yan, Yujie Zhang, Mei Yang, Yana Ma, Yuanyuan Zhang, Qiuran Xu, Kangsheng Tu, Mingzhen Zhang
Summary: The study suggests that TDNPs 2 derived from turmeric are a novel and natural colon-targeting therapeutics that may prevent colitis and promote wound repair in colitis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Cristian Hernandez-Rocha, Shadi Nayeri, Williams Turpin, Mike Steel, Krzysztof Borowski, Joanne M. Stempak, James Conner, Mark S. Silverberg
Summary: This study aimed to establish the prognosis, transcriptional and microbial features of histo-endoscopic remission and activity in ulcerative colitis (UC) patients. The results showed that UC patients with histo-endoscopic remission had a lower risk of relapse and their rectosigmoid mucosa transcriptional profile resembled that of healthy controls. Host genes with antimicrobial properties were significantly associated with mucosal microbiota.
JOURNAL OF CROHNS & COLITIS
(2022)
Article
Medicine, General & Internal
Fang Chen, Yue Hu, Yi-Hong Fan, Bin Lv
Summary: This study demonstrated the clinical significance of fecal calprotectin in evaluating endoscopic patterns and clinical manifestation in ulcerative colitis patients. The cut-off value of 164 μg/g for FC concentration was found to predict clinical active disease with high sensitivity and specificity. However, further large-scale studies are needed to confirm the reliability of the cut-off value.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Muniba Tariq, Hafiz Muhammad Tahir, Samima Asad Butt, Shaukat Ali, Asma Bashir Ahmad, Chand Raza, Muhammad Summer, Ali Hassan, Junaid Nadeem
Summary: The study found that the combination of 5% silk fibroin and 5% Aloe vera gel was the most effective in promoting wound healing, with wounds healing in 13 days and a wound contraction rate of 98.33%. Histological analysis showed increased blood vessel growth, collagen fibers, and reduced inflammation in wounds treated with silk formulations.
Article
Gastroenterology & Hepatology
Lorenzo Bertani, Brigida Barberio, Marco Fornili, Luca Antonioli, Federico Zanzi, Cesare Casadei, Laura Benvenuti, Sonia Facchin, Vanessa D'Antongiovanni, Greta Lorenzon, Linda Ceccarelli, Laura Baglietto, Nicola de Bortoli, Massimo Bellini, Francesco Costa, Edoardo Vincenzo Savarino, Matteo Fornai
Summary: This study suggests that serum oncostatin M is a drug-specific biomarker that can be used to predict therapeutic effectiveness in anti-TNF treatment. These results emphasize the utility of serum oncostatin M measurement in patients treated with anti-TNF.
DIGESTIVE AND LIVER DISEASE
(2022)
Article
Gastroenterology & Hepatology
Kana Shiraishi, Shinya Furukawa, Sen Yagi, Kenichirou Mori, Tomoyuki Ninomiya, Keitarou Kawasaki, Yuji Mizukami, Seiyuu Suzuki, Masayoshi Uraoka, Naozumi Shibata, Sanae Nakamura, Satoshi Imamine, Hidehiro Murakami, Katsuhisa Ohashi, Masamoto Torisu, Makoto Yano, Aki Hasebe, Harumi Yano, Masato Murakami, Eiji Takeshita, Yoshio Ikeda, Yoichi Hiasa
Summary: In patients with ulcerative colitis, serum globulin showed a significant positive association with endoscopic activity and a significant inverse association with mucosal healing, especially in the low C-reactive protein group.
DIGESTIVE DISEASES AND SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Tomohisa Takagi, Kazuhiko Uchiyama, Mariko Kajiwara-Kubota, Saori Kashiwagi, Yuki Toyokawa, Yuma Hotta, Makoto Tanaka, Ken Inoue, Osamu Dohi, Naohisa Yoshida, Kazuhiro Kamada, Takeshi Ishikawa, Hideyuki Konishi, Mitsuo Kishimoto, Nobuaki Yagi, Yuji Naito, Yoshito Itoh
Summary: The study investigated the efficacy of linked color imaging (LCI) in predicting the long-term prognosis of ulcerative colitis patients diagnosed with MES 0 over a 48-month period. Findings showed that LCI classification subdivided colonic mucosa diagnosed with MES 0 into two classes, with the LCI-A group having a significantly lower relapse rate than LCI-B group. This methodology can be used to evaluate mucosal healing and predict long-term outcomes in ulcerative colitis patients.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Pharmacology & Pharmacy
Qiubing Chen, Qian Li, Yuqi Liang, Menghang Zu, Nanxi Chen, Brandon S. B. Canup, Liyong Luo, Chenhui Wang, Liang Zeng, Bo Xiao
Summary: This study developed a new therapeutic strategy using natural nanovehicles derived from tea flowers to trigger apoptosis of breast tumors and inhibit their lung metastasis. The nanovehicles had desirable particle sizes, exosome-like morphology, and contained polyphenols, flavonoids, functional proteins, and lipids. The experiments showed that the nanovehicles exhibited strong cytotoxicity against cancer cells by generating reactive oxygen species (ROS). In addition, in mice investigations, the nanovehicles were found to accumulate in breast tumors and lung metastatic sites after injection or oral administration, resulting in inhibition of breast cancer growth and metastasis, as well as modulation of gut microbiota.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Engineering, Biomedical
Ya Ma, Lian Duan, Jianfeng Sun, Shuangquan Gou, Fengyuan Chen, Yuqi Liang, Fangyin Dai, Bo Xiao
Summary: This study developed engineered Antheraea pernyi silk fibroin nanoparticles loaded with an anti-inflammatory drug, resveratrol, to target colonic epithelial cells and macrophages for effective ulcerative colitis (UC) treatment. The nanoparticles showed lysosomal escape capacity, pH and reactive oxygen species responsiveness, and targeted delivery to the cytoplasm for on-demand drug release. Oral treatment with these nanoparticles relieved UC symptoms by restoring damaged colonic epithelial barriers, reducing inflammation, and rebalancing intestinal microbiota.
Review
Pharmacology & Pharmacy
Nanxi Chen, Jianfeng Sun, Zhenhua Zhu, Adam P. Cribbs, Bo Xiao
Summary: This article introduces the extraction, construction strategies, and therapeutic effects of plant-derived exosome-like NPs (PDENs) in various diseases. It also discusses the future direction and challenges of this technology.
EXPERT OPINION ON DRUG DELIVERY
(2022)
Article
Engineering, Biomedical
Xueqing Zhang, Qian He, Jianfeng Sun, Hanlin Gong, Yingui Cao, Lian Duan, Shixiong Yi, Binwu Ying, Bo Xiao
Summary: In this study, a near-infrared-driven nanomotor was developed for efficient penetration into tumor tissues and effective phototherapy. The nanomotor, coated with Antheraea pernyi silk fibroin, combined with chemotherapy drugs, achieved triple-modal treatment and activated systemic antitumor immunity by inducing immunogenic cell death and altering the tumor microenvironment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Genetics & Heredity
Minjin Han, Jianyu Ren, Haipeng Guo, Xiaoling Tong, Hai Hu, Kunpeng Lu, Zongrui Dai, Fangyin Dai
Summary: By studying the mutation rate and spectrum of the domestic silkworm under normal and two temperature stress conditions, it was found that the single-nucleotide mutation rate under normal temperature rearing condition was 1.5-fold higher than in previously studied insects. Moreover, the mutation rates of the silkworm under stress conditions were significantly higher than in normal conditions. Furthermore, the mutation rate varied less in gene regions under normal and temperature stresses. These findings expand the diversity of the mutation rate among eukaryotes and have implications for evolutionary analysis.
Editorial Material
Chemistry, Multidisciplinary
Chunhua Yang, Didier Merlin
Article
Chemistry, Multidisciplinary
Lingshuang Wang, Lian Duan, Ga Liu, Jianfeng Sun, Mohammad-Ali Shahbazi, Subhas C. C. Kundu, Rui L. L. Reis, Bo Xiao, Xiao Yang
Summary: Polyacrylic acid (PAA) and its derivatives are commonly used as matrices in wound dressings, but their weak wet adhesion limits their clinical application. In this study, a PAA-based coacervate hydrogel with strong wet adhesion capability is created by mixing PAA copolymers with isoprenyl oxy poly(ethylene glycol) ether and tannic acid. The coacervate exhibits solid adhesion to both dry and wet substrates, with an adhesive strength to skin of 23.4 kPa and 70% adhesion underwater. It also possesses desirable self-healing and extensible properties, making it suitable for joints that undergo frequent movement. The coacervate demonstrates antibacterial activity, facilitates fibroblast migration, modulates macrophage polarization, shortens hemostatic time, and promotes collagen deposition, angiogenesis, and epithelialization, suggesting its promising potential as a wound dressing in medical translation.
Article
Pharmacology & Pharmacy
Dingpei Long, Zahra Alghoul, Junsik Sung, Chunhua Yang, Didier Merlin
Summary: The researchers loaded the anti-cancer drug candidate M13 into colon-targeting ginger-derived nanoliposomes (NL) and investigated the enhanced anticancer effects of orally administered M13-NL on CAC in mouse models. M13 showed favorable physiochemical properties and no apparent immunotoxicity or mutagenic potential. The use of NL for drug delivery significantly improved the safety and efficacy of M13, and oral administration of M13-NL demonstrated excellent therapeutic effects in CAC mouse models.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Review
Pharmacology & Pharmacy
Cheng Xu, Haiting Xu, Zhenhua Zhu, Xiaoxiao Shi, Bo Xiao
Summary: Oral administration is the most common route for treating colonic diseases. However, the colonic mucus acts as a barrier that limits the effectiveness of orally administered drugs. Mucus-penetrating nanotherapeutics have been developed to overcome this barrier and improve treatment outcomes for colonic diseases.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Article
Pharmacology & Pharmacy
Dingpei Long, Zahra Alghoul, Junsik Sung, Chunhua Yang, Didier Merlin
Summary: Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease, increases the risk of colitis-associated cancer (CAC). The M13-nano-liposome (NL) formulation has been found to effectively prevent chronic UC. This study demonstrates that oral administration of M13-NL prevents tumor development in AOM-exposed IL10-/- mice, suggesting its potential for preventing CAC.
Article
Materials Science, Biomaterials
Kiyan Musaie, Samin Abbaszadeh, Vahideh Nosrati-Siahmazgi, Mostafa Qahremani, Shige Wang, Mohammad Reza Eskandari, Seyyed Vahid Niknezhad, Fakhri Haghi, Yulin Li, Bo Xiao, Mohammad-Ali Shahbazi
Summary: Photothermal therapy (PTT) is a promising cancer treatment approach, but it can lead to local lesions and bacterial infection. To address these challenges, a strategy of mild PTT following high-temperature tumor ablation was studied, using a hydrogel for combined tumor suppression, wound healing, and bacterial eradication. Bi2S3 nanorods (NRs) coated with hyaluronic acid were employed as a photothermal agent, loaded into a Fe3+-coordinated hydrogel composed of sodium alginate (Alg) and Farsi gum (FG). The hydrogel showed excellent blood clotting effect and antimicrobial activity, and achieved efficient photothermal-based tumor eradication and accelerated wound healing in vivo.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Ga Liu, Menghang Zu, Lingshuang Wang, Cheng Xu, Jiamei Zhang, Rui L. Reis, Subhas C. Kundu, Bo Xiao, Lian Duan, Xiao Yang
Summary: By constructing biocompatible CaO2-Cu2O micromotors, the wound healing process can be accelerated, exhibiting antibacterial and hemostatic functions, promoting cellular migration and modulating the inflammatory microenvironment, significantly improving the therapeutic effects on infected wounds.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Materials Science, Biomaterials
Chunyu Xie, Xiao Yang, Fan Zheng, Jiahao Shi, Caixia Huo, Zuyuan Wang, Rui L. Reis, Subhas C. Kundu, Bo Xiao, Lian Duan
Summary: Facilely printed regenerated silk fibroin (RSF) microparticles have great potential for facial rejuvenation, with desirable biocompatibility and lasting filling effects.
BIOMATERIALS SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Bili Zhang, Chunlin Li, Yue Luan, Yaru Lu, Hai Hu, Yanyu Liu, Kunpeng Lu, Guizheng Zhang, Fangyin Dai, Xiaoling Tong
Summary: In this study, nine HEX genes in Bombyx mori were identified and their distinct expression patterns were analyzed. The chitooligosaccharidolytic beta-N-acetylglucosaminidase (BmChiNAG) gene was found to be highly expressed in chitin-rich tissues and played a crucial role in molting and wing development in silkworms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)