期刊
PLANT REPRODUCTION
卷 32, 期 4, 页码 371-380出版社
SPRINGER
DOI: 10.1007/s00497-019-00377-6
关键词
Soybean [Glycine max (L; ) Merr; ]; Nuclear male sterility (NMS); Simple sequence repeats (SSR); Gene mapping
资金
- China National Key RD program [2016YFD0100304, 2017YFD0102002, 2017YFD0101500]
- MOE 111 Project [B08025]
- MOE Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT_17R55]
- MARA CARS-04 program
- Jiangsu Higher Education PAPD Program
- Fundamental Research Funds for the Central Universities [KYT201801]
- Jiangsu JCIC-MCP
Nuclear male sterility (NMS) is a potential characteristic in crop recurrent selection and hybrid breeding. Mapping of nuclear male-sterile genes is key to utilizing NMS. Previously, we discovered a spontaneous soybean (Glycine max [L.] Merr.) male-sterile female-fertile mutant NJS-13H, which was conferred by a single recessive gene, designated ms(NJ). In this study, the ms(NJ) was mapped to Chromosome 10 (LG O), and narrowed down between two SSR (simple sequence repeats) markers, BARCSOYSSR_10_794 and BARCSOYSSR_10_819 using three heterozygote-derived segregating populations, i.e., (NJS-13H x NN1138-2)F-2, (NJS-13H x N2899)F-2 and (NJS-13H)SPAG (segregating populations in advanced generations). This region spans approximately 1.32 Mb, where 27 genes were annotated according to the soybean reference genome sequence (Wm82.a2.v1). Among them, four genes were recognized as candidate genes for ms(NJ). Comparing to the physical locations of all the known male-sterile loci, ms(NJ) is demonstrated to be a new male-sterile locus. This result may help the utilization and cloning of the gene.
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