期刊
BIOPHYSICAL JOURNAL
卷 109, 期 5, 页码 892-899出版社
CELL PRESS
DOI: 10.1016/j.bpj.2015.07.039
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资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01020108]
- Ministry of Science and Technology 973 program [2013CB967403, 2012CB721105]
- Ministry of Science and Technology 863 Program [2012AA02A708]
- National Natural Science Foundation projects of China [31271527]
- Guangzhou Science and Technology Program [2014Y2-00161]
- Guangdong Natural Science Foundation for Distinguished Young Scientists [S20120011368]
- Guangdong Natural Science Foundation for PhD Start-up [2014A030310071]
- One Hundred Talents Project for Professor Xingguo Liu from the Chinese Academy of Sciences
- Guangdong Province Science and Technology Innovation Young Talents Program [2014TQ01R559]
Mitochondria are highly dynamic cell organelles. Continual cycles of fusion and fission play an important role in mitochondrial metabolism and cellular signaling. Previously, a novel mitochondrial morphology, the donut, was reported in cells after hypoxia-reoxygenation or osmotic pressure changes. However, the mechanism of donut formation remained elusive. Here, we obtained the distribution of donut diameters (D = 2R) and found that 95% are >0.8 mm. We also performed highly precise measurements of the mitochondrial tubule diameters using superresolution and electron microscopy. Then, we set up a model by calculating the mitochondrial bending energy and osmotic potential during donut formation. It shows that the bending energy is increased as the radius of curvature, R, gets smaller in the process of donut formation, especially for radii <0.4 mm, creating a barrier to donut formation. The calculations also show that osmotic potential energy release can balance the rising bending energy through volume expansion. Finally, we revealed the donut formation process in a Gibbs free-energy-dependent model combining calculations and measurements.
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