Article
Biochemistry & Molecular Biology
Daniel Gao, Caroline W. Sun, Angela B. Woodley, Jing-fei Dong
Summary: Clot retraction, which requires the functional platelet alpha(IIb)beta(3) integrin, is an important measure of platelet function and can be used to detect functional changes in platelets. Platelet counts and the surface density of the integrin alpha(IIb)beta(3) affect clot retraction.
Article
Pharmacology & Pharmacy
Gi Suk Nam, Soyoung Kim, Yun-Suk Kwon, Min-Kyung Kim, Kyung-Soo Nam
Summary: MAP4K4 inhibitors reduce thrombus formation by regulating platelet activation, showing potential for the treatment of thrombosis.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Cell Biology
Wayne T. Muraoka, Prajeeda M. Nair, Daniel N. Darlington, Xiaowu Wu, James A. Bynum, Andrew P. Cap
Summary: This study describes a 96-well-based clot retraction assay that allows for rapid measurement of platelet function and provides quantitative information. The assay is robust to different calcium concentrations and can predict factors affecting platelet function.
Article
Hematology
Karen P. Fong, Kathleen S. Molnar, Nicholas Agard, Rustem Litvinov, Oleg Kim, James A. Wells, John W. Weisel, William F. DeGrado, Joel S. Bennett
Summary: Blood clot contraction is driven by traction forces generated by the platelet cytoskeleton and regulated by integrin alpha IIb beta 3. Proteolytically cleaved platelet proteins, particularly talin and vinculin, play crucial roles in clot contraction. Calpain-mediated cleavage of these proteins occurs independent of cytoskeletal-generated tensile forces, suggesting calpain's involvement in later stages of clot retraction through focal adhesion disassembly.
Article
Biochemical Research Methods
Lancong Liu, Wenfeng Ding, Lili He, Yi Yang, Fuyi Guan, Xinlin Sun, Yan Peng, Xue Chen, Wenhao Zhao, Yu Xiao, Pei Luo
Summary: We synthesized a novel peptide, WK001, by modifying the RGD structure to combine with scutellarin. Experimental and docking evidence showed that WK001 provides immediate and potent platelet inhibition, surpassing the inhibition of eptifibatide and scutellarin in platelet aggregation. In vivo studies demonstrated that WK001 administered intravenously prevents thrombus formation and attenuates myocardial fibrosis progression. Therefore, WK001 has the potential to be developed as an antiplatelet drug for thrombosis-associated diseases, such as stroke and myocardial infarction.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Hematology
Alan T. Nurden
Summary: Clot retraction is essential for preventing bleeding, thrombosis, and tissue repair. The process involves complex molecular mechanisms, including platelet adhesion, aggregation, thrombin generation, and fibrin production. Platelet contractile activity and crosslinking consolidate a fibrin mesh, providing stability and resistance to fibrinolysis. Key players in this process are integrin alpha IIb beta 3 on platelets. Clot construction and retraction are influenced by platelet cytoskeleton, actomyosin, rheological factors, and environmental conditions. Activated platelets release procoagulant proteins and substances, promoting thrombin generation and wound healing by facilitating stem cell recruitment, cell migration, angiogenesis, and matrix formation.
THROMBOSIS RESEARCH
(2023)
Article
Hematology
Ana Bura, Maria Antonietta de Matteis, Markus Bender, Maurice Swinkels, Jurjen Versluis, A. J. Gerard Jansen, Antonija Jurak Begonja
Summary: Lowe syndrome is a rare disorder that affects the brain, eyes, and kidneys. It is caused by mutations in the OCRL protein. Some patients with Lowe syndrome have bleeding disorders and impaired platelet function. Experiments show that OCRL inhibition affects cytoskeletal rearrangement in platelet activation, which may explain the mild bleeding problems in patients with Lowe syndrome.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Cell Biology
Takeshi Sugimoto, Hiroyuki Yamada, Naotoshi Wada, Shinichiro Motoyama, Makoto Saburi, Hiroshi Kubota, Daisuke Miyawaki, Noriyuki Wakana, Daisuke Kami, Takehiro Ogata, Masakazu Ibi, Satoaki Matoba
Summary: This study investigated the impact of repeated social defeat (RSD) on arterial thrombosis and found that RSD enhances the formation of fibrin-rich clots after arterial injury. These findings suggest that NET could be a new therapeutic target in depression-related cardiovascular disease.
Article
Hematology
Jianmin Liu, Fan Lu, Sujay Subbayya Ithychanda, Marcin Apostol, Mitali Das, Gauravi Deshpande, Edward F. Plow, Jun Qin
Summary: This study reveals that filamin associates with both inactive and active forms of alpha IIb ss 3, promoting platelet spreading. Furthermore, the study demonstrates that filamin undergoes spatiotemporal rearrangement upon alpha IIb ss 3 activation, binding to the alpha IIb cytoplasmic tail alone. This novel alpha IIb CT-filamin-actin linkage plays a crucial role in integrin outside-in signaling.
Article
Hematology
Joanne L. Mitchell, Gemma Little, Alexander P. Bye, Renato S. Gaspar, Amanda J. Unsworth, Neline Kriek, Tanya Sage, Alexander Stainer, Ibidayo Sangowawa, Gael B. Morrow, Ricardo N. Bastos, Susan Shapiro, Michael J. R. Desborough, Nicola Curry, Jonathan M. Gibbins, Claire S. Whyte, Nicola J. Mutch, Christopher I. Jones
Summary: This study identifies the role of platelet FXIII-A in platelet function. Platelet FXIII-A enhances fibrinogen binding to the platelet surface and improves platelet aggregation and thrombus formation. FXIII-A is also involved in platelet spreading and clot retraction.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Lucy J. Goudswaard, Christopher M. Williams, Jawad Khalil, Kate L. Burley, Fergus Hamilton, David Arnold, Alice Milne, Phil A. Lewis, Kate J. Heesom, Stuart J. Mundell, Andrew D. Davidson, Alastair W. Poole, Ingeborg Hers
Summary: Patients with COVID-19 have altered platelet function, which is associated with increased risk of thrombosis. Platelet protein expression is different in COVID-19 patients, and platelet functional responses are reduced. Platelets in COVID-19 patients also show increased association with neutrophils. These findings suggest that platelet-driven thromboinflammation may contribute to the increased risk of thrombosis in COVID-19 patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Integrative & Complementary Medicine
Jung-Hae Shin, Muhammad Irfan, Man Hee Rhee, Hyuk-Woo Kwon
Summary: Cudrania tricuspidata extract, specifically derrone, has shown antiplatelet effects and inhibited thrombus formation by affecting various signaling molecules involved in platelet function. This suggests potential therapeutic applications in conditions related to platelet aggregation and clot formation.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lu Gong, Samuel Bates, Jian Li, Dandi Qiao, Kimberly Glass, Wenyi Wei, Victor W. Hsu, Xiaobo Zhou, Edwin K. Silverman
Summary: The study identified a cellular protein complex composed of TGF beta 2, FAM13A, and AP3D1 which mediates TGF beta 2 secretion through an AP-3 dependent pathway, with FAM13A acting as a negative regulator. This reveals the biological network roles of two COPD GWAS proteins and novel mechanisms of cargo transport through an intracellular pathway.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Hematology
Kui Liu, Zhen Hao, Hao Zheng, Haojie Wang, Luying Zhang, Minghui Yan, Reyisha Tuerhong, Yuling Zhou, Yan Wang, Tao Pang, Lei Shi
Summary: In this study, it was found that rilpivirine (RPV) effectively inhibited platelet aggregation, prevented thrombus formation, and improved cardiac function without side effects. These results suggest that RPV could be a promising agent for the prevention and therapy of thrombotic cardiovascular diseases.
THROMBOSIS RESEARCH
(2023)
Article
Integrative & Complementary Medicine
Bo-Ra Jeon, Muhammad Irfan, Seung Eun Lee, Jeong Hoon Lee, Man Hee Rhee
Summary: The study found that Rumex acetosella extract has inhibitory effects on platelet activity and can exert its effects by modulating signaling pathways. This suggests that Rumex acetosella may have a protective role in the development of cardiovascular diseases.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2022)