Article
Plant Sciences
Gangming Zhan, Fan Ji, Jun Zhao, Yue Liu, Aihong Zhou, Minghao Xia, Juntian Zhang, Lili Huang, Jun Guo, Zhensheng Kang
Summary: Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious threat to wheat production safety worldwide. In this study, the distribution of triadimefon-resistant Pst isolates in China was investigated, and the sensitivity of Pst to triadimefon was evaluated. The results showed a wide range of sensitivity to triadimefon, indicating a certain risk of resistance.
Article
Agronomy
Nicola M. Cook, Soonie Chng, Tamsin L. Woodman, Rachael Warren, Richard P. Oliver, Diane G. O. Saunders
Summary: The study revealed that mutations associated with fungicide resistance have been found in the wheat yellow rust pathogen in certain locations, indicating high levels of selection for resistance evolution in cereal rust species.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Manamele Dannies Mashabela, Fidele Tugizimana, Paul Anton Steenkamp, Lizelle Ann Piater, Ian Augustus Dubery, Msizi Innocent Mhlongo
Summary: This study used LC-MS technology to analyze the metabolomic responses of Pst-resistant and susceptible wheat varieties to P. striiformis infection. The results showed that wheat plants undergo time-dependent metabolic reprogramming in response to the pathogen, with changes in phenylpropanoids, amino acids, lipids, benzoic acids, TCA cycle intermediates, and benzoxazinoids. The resistant cultivar Koonap accumulated defense metabolites more rapidly than the susceptible Gariep cultivar, suggesting the potential for metabolic engineering to improve plant tolerance and resistance to biotic stresses.
Article
Plant Sciences
Gensheng Zhang, Wei Liu, Lin Wang, Xiangrui Cheng, Xiaxia Tian, Zhimin Du, Zhensheng Kang, Jie Zhao
Summary: This study found that Chinese wheat cultivars have varying levels of resistance to the new races TSA-6 and TSA-9. Some cultivars showed resistance to both races. Additionally, certain cultivars exhibited adult plant resistance and slow rusting resistance to these races.
Article
Biochemistry & Molecular Biology
Mehari Desta Hawku, Fuxin He, Xingxuan Bai, Md Ashraful Islam, Xueling Huang, Zhensheng Kang, Jun Guo
Summary: The MYB transcription factor TaMYB391 plays a crucial role in the interaction between wheat and the stripe rust fungus, contributing to the resistance of wheat through the regulation of certain PR genes, possibly through the SA signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Aihong Zhou, Yaoxuan Feng, Xinpei Gao, Yue Liu, Fan Ji, Lili Huang, Zhensheng Kang, Gangming Zhan
Summary: This study investigated the sensitivity of wheat stripe rust isolates to triadimefon in different regions of China. It was found that isolates from Xinjiang exhibited strong resistance to triadimefon. The resistant isolates had weaker adaptive traits compared to sensitive isolates. Additionally, a mutation was identified in the isolates from Xinjiang. This study reveals the reasons behind the loss of efficacy of triadimefon against wheat stripe rust.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Plant Sciences
Junyan Feng, Fangjie Yao, Meinan Wang, Deven R. See, Xianming Chen
Summary: Wheat stripe rust, one of the most serious plant diseases worldwide, can be effectively controlled by breeding resistant cultivars. In this study, researchers successfully mapped the YrTr1 gene, an important resistance gene, to the short arm of chromosome 1B in wheat, and named it Yr85.
Article
Agriculture, Multidisciplinary
Shi Bei-bei, Juan Wang, Hai-feng Gao, Xiao-juan Zhang, Yang Wang, Qing Ma
Summary: The TaFIM1 gene in wheat plays a positive regulatory role in pathogen resistance and response to abiotic stress, with silencing TaFIM1 leading to a reduction in resistance against stripe rust. These findings shed new light on the role of fimbrin in wheat.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Mehari Desta Hawku, Farhan Goher, Md Ashraful Islam, Jia Guo, Fuxin He, Xingxuan Bai, Pu Yuan, Zhensheng Kang, Jun Guo
Summary: This study reveals the role of TaAP2-15 in the resistance of wheat to the stripe rust fungus, showing that it positively regulates the expression levels of pathogenesis-related genes and reactive oxygen species-scavenging genes to affect pathogen growth. Additionally, TaAP2-15 interacts with a zinc finger protein, TaRZFP34, providing new insights into the host-pathogen interaction mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Meinan Wang, Anmin Wan, Xianming Chen
Summary: In this study, 1,567 isolates of Puccinia striiformis f. sp. tritici collected from the United States between 2013 and 2017 were tested for virulence. The study identified 72 races, including 20 new races. The frequencies of these races varied in different years and regions. The findings provide valuable insights into the evolution of the pathogen and the development of resistant wheat cultivars.
Article
Plant Sciences
Cai Sun, Yike Liu, Qiang Li, Baotong Wang, Shuhui Chen, Jianxin Deng, Dongfang Ma, Yuheng Yang
Summary: In this study, a dominant gene YrXk conferring resistance to Puccinia striiformis f. sp. tritici race CYR33 was identified in Chinese wheat cultivar Xike01015. The gene was mapped to a specific region on chromosome BS and three candidate genes were identified. The findings provide useful information for wheat breeding programs aiming to enhance resistance to stripe rust.
Article
Plant Sciences
Yuxiang Li, Lu Liu, Meinan Wang, Travis Ruff, Deven R. See, Xiaoping Hu, Xianming Chen
Summary: A mutant wheat line, M1225, with high-temperature adult-plant resistance to Puccinia striiformis f. sp. tritici, was obtained through crossbreeding and genetic analysis revealed that the HTAP resistance gene is located on the short arm of chromosome 2A within a 7.5 centimorgan interval.
Article
Plant Sciences
Xiao-Ming Li, Chu-Yang Wang, Yue-Ting Guo, Yu-Xin Guo, Han-Xiao Du, Ke-Xin Niu, Yan Yan, Jin-Ying Gou
Summary: This study investigated the transcriptome response of wheat seedlings to five phytohormones and developed fluorescent reporters to monitor the dynamics of jasmonate.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Md. Ashraful Alam, Haoxing Li, Akbar Hossain, Mingju Li
Summary: The research found that the genetic diversity of wheat stripe rust pathogen in most regions of Yunnan Province is high, but the genetic differences between regions are small, indicating frequent migration of the pathogen within the province. Importantly, Dehong, Dali, Lincang, and Baoshan are likely major sources of wheat stripe rust in Yunnan Province.
Article
Biochemistry & Molecular Biology
Qing Bai, Anmin Wan, Meinan Wang, Deven R. See, Xianming Chen
Summary: The study revealed that the stripe rust pathogen is migrating between different countries with a certain level of genetic diversity. Most molecular groups were found to be involved in sexual and/or somatic recombination, indicating genetic mixing among different countries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Yuanyuan Zhao, Xueling Huang, Qiao Li, Lili Huang, Zhensheng Kang, Jie Zhao
Summary: The emergence of new Puccinia striiformis f. sp. tritici races overcoming wheat resistance is a challenging issue. In a study conducted in Shaanxi Province, China, the phenotypic and genotypic diversity of the pathogen on barberry plants and its relationship with the population in wheat fields were determined. A total of 120 races and 132 genotypes were identified, indicating a close relationship and strong gene flow between the populations on barberry and wheat. This study highlights the important role of barberry plants in the epidemiology and management of stripe rust.
Article
Plant Sciences
Gensheng Zhang, Wei Liu, Lin Wang, Meng Ju, Xiaxia Tian, Zhimin Du, Zhensheng Kang, Jie Zhao
Summary: Through analyzing the avirulence and virulence of 120 selfed progeny lines from Berberis shensiana, we found that the TSA-6 Puccinia striiformis f. sp. tritici (Pst) isolate is virulent against the Yr5 resistance gene and exhibited 73 different virulence phenotypes compared to the parental isolate. Further analysis revealed the genetic composition of the TSA-6 isolate at various resistance loci and identified quantitative trait loci associated with virulence at 20 Yr resistance loci. These findings contribute to understanding Pst virulence evolution and simplifying breeding for wheat cultivars with effective resistance to wheat stripe rust.
Article
Plant Sciences
Zhimin Du, Yuelin Peng, Gensheng Zhang, Li Chen, Shuchang Jiang, Zhensheng Kang, Jie Zhao
Summary: This study revealed that the wheat stripe rust pathogen can undergo sexual reproduction on susceptible barberry in late autumn in Tibet, China. The findings contribute to the understanding of wheat stripe rust and provide insights into disease management in the region.
Article
Biochemistry & Molecular Biology
Jinping Wei, Xiaodong Wang, Zeyu Hu, Xiaojie Wang, Jialiu Wang, Jianfeng Wang, Xueling Huang, Zhensheng Kang, Chunlei Tang
Summary: The obligate biotrophic fungus Puccinia striiformis f. sp. tritici (Pst) employs the effector Hasp98 to inhibit wheat's immune responses and promote the infection of P. striiformis. Hasp98 interacts with the wheat protein TaMAPK4, inhibiting its kinase activity and interfering with the MAPK signaling pathway. This study provides insights into how Pst effectors regulate host defense responses and promote stripe rust disease.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Linying Du, Xueling Huang, Li Ding, Zhongxue Wang, Dongling Tang, Bin Chen, Lanjiya Ao, Yuling Liu, Zhensheng Kang, Hude Mao
Summary: This study reveals the important role of TaERF87 in regulating drought tolerance in wheat. TaERF87 enhances drought tolerance by activating the expression of proline biosynthesis genes and synergistically interacts with TaAKS1. These findings contribute to the development of elite drought-tolerant wheat varieties.
Article
Biochemistry & Molecular Biology
Xiaobo Wei, Xueling Huang, Weiling Yang, Xinran Wang, Tao Guan, Zhensheng Kang, Jie Liu
Summary: This study cloned and identified a wheat G6DPH gene, TaG6PDH2, which is significantly upregulated in wheat leaves infected with Puccinia striiformis f. sp. tritici (Pst) or treated with abiotic stress factors. Functional analysis revealed that TaG6PDH2 encodes a chloroplast-targeting G6PDH protein. Silencing TaG6PDH2 compromised wheat resistance to Pst avirulent pathotype CYR23, and TaG6PDH2 was found to interact with wheat glutaredoxin TaGrxS4.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yingchao Sun, Xiaojie Wang, Feiyang Liu, Haoyu Guo, Jianfeng Wang, Zetong Wei, Zhensheng Kang, Chunlei Tang
Summary: Plant cell surface RLKs play a crucial role in recognizing and responding to pathogen invasion. In this study, a specific RLK gene (TaBIR1) was found to be upregulated during wheat resistance to Puccinia striiformis f. sp. tritici (Pst) infection. TaBIR1 was shown to be located on the plasma membrane and its knockdown resulted in decreased wheat resistance to stripe rust. Overexpression of TaBIR1 triggered constitutive immune responses in Nicotiana benthamiana. Moreover, TaBIR1's immune response is dependent on the homologues of NbBAK1 in wheat.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Fan Ji, Aihong Zhou, Bofan Liu, Yue Liu, Yaoxuan Feng, Xiaodong Wang, Lili Huang, Zhensheng Kang, Gangming Zhan
Summary: This study assessed the resistance risk of wheat leaf rust to the fungicide triadimefon in China. The majority of tested Pt isolates were sensitive to triadimefon, but 10.2% exhibited varying degrees of resistance. The resistant isolates showed strong adaptive traits in parasitic fitness. No correlation was found between triadimefon and other fungicides with similar or different modes of action. The risk of resistance to triadimefon in Pt may be low to moderate.
Article
Virology
Yanhui Zhang, Hualong Guo, Siyu Zhou, Daipeng Chen, Gang Xu, Zhensheng Kang, Li Zheng
Summary: We report a new mitovirus, named Puccinia striiformis mitovirus 2 (PsMV2), from the wheat stripe rust fungus. PsMV2 exhibited high multiplication during Pst infection and suppressed programmed cell death triggered by Bax. Silencing of PsMV2 reduced fungal growth and pathogenicity of Pst. Our results indicate that PsMV2 promotes pathogenicity and has a wide distribution in field isolates of Pst.
Article
Biochemistry & Molecular Biology
Jia Guo, Ying Mou, Yuanxing Li, Qing Yang, Xue Wang, Haocheng Lin, Zhensheng Kang, Jun Guo
Summary: This study identified 131 chitinase genes in 10 fungal genomes and found 16 chitinase genes from Puccinia striiformis f. sp. tritici (Pst). The expression of PstChia1 was the highest during Pst infection and it showed chitinase enzyme activity. Silencing PstChia1 limited the growth and reduced the virulence of Pst, indicating its role in pathogenicity and interference with plant immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Muye Xiao, Dezhi Chen, Saifei Liu, Anle Chen, Anfei Fang, Binnian Tian, Yang Yu, Chaowei Bi, Zhensheng Kang, Yuheng Yang
Summary: A chitin deacetylase called PsCDA2 was identified in the wheat stripe rust fungus Pst, which inhibits plant cell death and protects the fungus from host chitinase. This discovery provides insights into the mechanism of plant immune suppression and has implications for wheat stripe rust control.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Wanlu Duan, Zhenkai Hao, Huihui Pang, Yuxi Peng, Yiwen Xu, Yanfei Zhang, Ying Zhang, Zhensheng Kang, Jing Zhao
Summary: Regulation of host gene expression by fungal pathogens is an important strategy for promoting disease. In this study, a fungal effector called PstGTA1 was identified from Puccinia striiformis f. sp. tritici (Pst). It was found to have transcriptional activating activity and contribute to the virulence of Pst by targeting the promoter of a wheat gene called TaSIG. This study provides the first evidence for a fungal effector modulating host gene transcription through promoter binding and histone acetylation.
Article
Microbiology
Mengxin Zhao, Yanhui Zhang, Hualong Guo, Pengfei Gan, Mengmeng Cai, Zhensheng Kang, Yulin Cheng
Summary: This study identified and analyzed the function of CAP superfamily proteins in the wheat stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). The results showed that PsCAP1, PsCAP4, and PsCAP5 play significant roles in Pst infection in wheat.
Article
Microbiology
Chengcai Yan, Haiting Hao, Zhe Wang, Shuaishuai Sha, Yiwen Zhang, Qingpeng Wang, Zhensheng Kang, Lili Huang, Lan Wang, Hongzu Feng
Summary: Years of outbreaks of woody canker (Cryptosphaeria pullmanensis) in the United States, Iran, and China have resulted in massive economic losses. Utilizing the MaxEnt model, the spatiotemporal distribution of C. pullmanensis was predicted under twelve climate change scenarios. C. pullmanensis is mostly widespread in high-suitability regions, but suitable land areas are diminishing globally. Migration trend is towards higher latitudes and elevations, and China's estimated suitable area shifted eastward. The results of this study are valuable for countries where the infection has not yet fully spread and for nations where the species has been discovered.
Article
Plant Sciences
Cuiping Wan, Yan Liu, Shuxin Tian, Jia Guo, Xingxuan Bai, Haochuan Zhu, Zhensheng Kang, Jun Guo
Summary: The wheat stripe rust pathogen utilizes a serine-rich effector to suppress plant immunity by targeting a susceptibility kinase. The effector interacts with the Raf-like kinase TaRaf46 to promote infection in wheat, while knocking down TaRaf46 expression enhances wheat immunity against the pathogen.