Article
Biochemistry & Molecular Biology
Amen Keshawarz, Roby Joehanes, Jiantao Ma, Gha Young Lee, Ricardo Costeira, Pei-Chien Tsai, Olatz M. Masachs, Jordana T. Bell, Rory Wilson, Barbara Thorand, Juliane Winkelmann, Annette Peters, Jakob Linseisen, Melanie Waldenberger, Terho Lehtimaeki, Pashupati P. Mishra, Mika Kahonen, Olli Raitakari, Mika Helminen, Carol A. Wang, Phillip E. Melton, Rae-Chi Huang, Craig E. Pennell, Therese A. O'Sullivan, Carolina Ochoa-Rosales, Trudy Voortman, Joyce B. J. van Meurs, Kristin L. Young, Misa Graff, Yujie Wang, Douglas P. Kiel, Caren E. Smith, Paul F. Jacques, Daniel Levy
Summary: Through meta-analysis of epigenome-wide association studies, we found significant associations between dietary and supplemental intake of vitamins C and E with DNA methylation. Vitamin C intake was associated with 4,656 CpG sites, while vitamin E intake was associated with 160 CpG sites. Vitamin C intake was found to be related to systems development and the immune response, while no significant associations were found for vitamin E intake with any biological pathways investigated.
Review
Oncology
Jyoti Shekhawat, Kavya Gauba, Shruti Gupta, Bikram Choudhury, Purvi Purohit, Praveen Sharma, Mithu Banerjee
Summary: TET enzymes play a central role in maintaining methylation balance, and anomalies in their expression may contribute to cancer progression. Low levels of TET enzymes and 5-hmC are associated with tumor invasion, progression, and metastasis. Additionally, the use of vitamin C enhances TET activity.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Medicine, General & Internal
Pau Montesinos, Christian Recher, Susana Vives, Ewa Zarzycka, Jianxiang Wang, Giambattista Bertani, Michael Heuser, Rodrigo T. Calado, Andre C. Schuh, Su-Peng Yeh, Scott R. Daigle, Jianan Hui, Shuchi S. Pandya, Diego A. Gianolio, Stephane de Botton, Hartmut Dohner
Summary: This study demonstrated that the combination therapy of oral ivosidenib and azacitidine showed better event-free survival and longer overall survival in patients with newly diagnosed IDH1-mutated acute myeloid leukemia.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Oncology
Tiziana Ottone, Isabella Faraoni, Giorgio Fucci, Mariadomenica Divona, Serena Travaglini, Eleonora De Bellis, Francesco Marchesi, Daniela Francesca Angelini, Raffaele Palmieri, Carmelo Gurnari, Manuela Giansanti, Anna Maria Nardozza, Federica Montesano, Emiliano Fabiani, Elisa Linnea Lindfors Rossi, Raffaella Cerretti, Laura Cicconi, Marco De Bardi, Maria Luisa Catanoso, Luca Battistini, Renato Massoud, Adriano Venditti, Maria Teresa Voso
Summary: This study found that vitamin C levels are significantly lower in AML patients at the time of initial diagnosis, further decreasing during disease progression, and returning to normal upon achievement of complete remission. Additionally, vitamin C levels are significantly lower in AML cells compared to healthy cells. However, there is no association between vitamin C levels and the mutation profile, cytogenetics, or risk stratification of AML patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tyler C. Huff, David W. Sant, Vladimir Camarena, Derek Van Booven, Nadja S. Andrade, Sushmita Mustafi, Paula Monje, Gaofeng Wang
Summary: Vitamin C plays a critical role in promoting myelination by Schwann cells through increasing DNA demethylation and enhancing the transcription of pro-myelinating genes.
JOURNAL OF NEUROCHEMISTRY
(2021)
Review
Oncology
Manuela Giansanti, Terry Karimi, Isabella Faraoni, Grazia Graziani
Summary: Vitamin C at high doses may have beneficial effects in killing tumor cells, with diverse mechanisms of action and potential for personalized treatment in combination with other drugs, offering new therapeutic opportunities for patients with advanced tumors who failed previous treatment with chemotherapy.
Review
Chemistry, Multidisciplinary
Chen-chen Li, Hui-yan Chen, Yue-hong Dong, Xiliang Luo, Juan Hu, Chun-yang Zhang
Summary: 5-Hydroxymethylcytosine (5hmC) is a recently discovered epigenetic modification that is closely related to tumorigenesis, developmental diseases, and nervous system diseases. Newly developed detection methods have facilitated the accurate detection of 5hmC and advanced research in epigenetics.
ACTA CHIMICA SINICA
(2021)
Article
Oncology
Jinshou Yang, Bo Ren, Jie Ren, Gang Yang, Yuan Fang, Xing Wang, Feihan Zhou, Lei You, Yupei Zhao
Summary: Metabolic remodelling and epigenetic reprogramming play important roles in PDAC tumorigenesis and metastasis. This study focused on guanidinoacetic acid (GAA) and revealed its association with PDAC liver metastasis through the GAA-c-Myc-HMGA axis and transcription-activating histone modifications reprogramming.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Huideng Long, Yue Hou, Jun Li, Chunhua Song, Zheng Ge
Summary: The combination therapy of KPT-330 with AZA showed synergistic effects in acute myeloid leukemia (AML) by suppressing the XPO1/eIF4E/c-MYC signaling pathway, reducing cell proliferation, and inducing apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Fu Zhao, Zhi-Wei Zhang, Jing Zhang, Shun Zhang, Heng Zhang, Chi Zhao, Yang Chen, Lin Luo, Wei-Min Tong, Chunde Li, Yamei Niu, Pinan Liu
Summary: The study revealed that decreased global 5hmC levels in medulloblastoma patients were associated with poor prognosis, as well as cell proliferation and TET protein expression.
FRONTIERS IN ONCOLOGY
(2021)
Review
Nutrition & Dietetics
Sascha Venturelli, Christian Leischner, Thomas Helling, Markus Burkard, Luigi Marongiu
Summary: The imbalance of micronutrients in the Western-style diet may lead to cellular damage and increase the risk of cancer, highlighting the need for more data to assess the effectiveness of vitamins as biomarkers in cancer.
Article
Oncology
Jie Qi, Yue Shi, Yezhen Tan, Qi Zhang, Jianye Zhang, Jilu Wang, Cong Huang, Weimin Ci
Summary: This study analyzed the changes of DNA 5-hydroxymethylcytosine (5hmC) in genitourinary cancers and found tissue-specific distribution of 5hmC as well as regional gain and global loss of 5hmC in these cancers. The genes with gain of 5hmC were functionally enriched in stemness and hypoxia, and associated with poor clinical prognosis. The study also discovered that an oxidation-resistant vitamin C derivative restored 5hmC and induced apoptosis in cancer stem cell-like cells.
CLINICAL EPIGENETICS
(2022)
Article
Biology
Heng-Yi Chen, Ana Almonte-Loya, Fang-Yun Lay, Michael Hsu, Eric Johnson, Edahi Gonzalez-Avalos, Jieyun Yin, Richard S. Bruno, Qin Ma, Hazem E. Ghoneim, Daniel J. Wozniak, Fiona E. Harrison, Chan-Wang Jerry Lio
Summary: Vitamin C is an essential micronutrient that enhances antibody response by promoting plasma cell differentiation through IL-21/STAT3 signaling pathway. As a cofactor for epigenetic enzymes, it facilitates DNA modification and demethylation, thereby influencing gene expression and cell fate decisions in the immune system.
Review
Biochemistry & Molecular Biology
Janos Hunyady
Summary: Vitamin C has both antioxidant and pro-oxidant properties, and it exhibits cytotoxic effects on cancer cells. However, the therapeutic effect of high-dose intravenous vitamin C treatment in cancer patients is currently ambiguous based on clinical evidence. Further research is needed to evaluate its clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Nasim Azizgolshani, Curtis L. Petersen, Youdinghuan Chen, Joshua J. Levy, Lucas A. Salas, Laurent Perreard, Lananh N. Nguyen, Brock C. Christensen
Summary: This study focused on nucleotide-specific 5hmC in pediatric central nervous system tumors, revealing that tumors generally exhibit lower levels of 5hmC throughout the epigenome. The research also demonstrated that tumor 5hmC is localized to key regulatory elements involved in cell identity. Furthermore, differences in hydroxymethylated loci among tumor subtypes were found to be hypermethylated and disproportionately located in CTCF binding sites and genes related to posttranscriptional RNA regulation. Additionally, patients with low 5hmC patterns showed poorer overall survival and higher risk of recurrence.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Josephine Skat-Rordam, Kamilla Pedersen, Gry Freja Skovsted, Ida Gregersen, Sara Vangsgaard, David H. Ipsen, Markus Latta, Jens Lykkesfeldt, Pernille Tveden-Nyborg
Summary: The study suggests that inadequate vitamin C intake may delay recovery in non-alcoholic fatty liver disease (NAFLD) and steatosis (NASH). A diet change combined with high vitamin C intake improves hepatic histopathological hallmarks, but vitamin C deficiency alone does not accelerate disease progression.
Review
Oncology
Djordje Marina, Ase Krogh Rasmussen, Kristian Buch-Larsen, Linn Gillberg, Michael Andersson, Peter Schwarz
Summary: Tamoxifen treatment in breast cancer patients may lead to mild and transient thyroid dysfunction, while letrozole treatment does not appear to have significant effects. Current literature has not focused on subclinical hypothyroidism as a potential cause of weight gain in breast cancer patients.
Article
Oncology
Agnete Marie Granau, Pilar Aaroe Andersen, Theresa Jakobsen, Konstantina Taouxi, Nawar Dalila, Johanne Bay Mogensen, Lasse Sommer Kristensen, Kirsten Gronbaek, Konstantinos Dimopoulos
Summary: Targeting BCL-2 through venetoclax is an effective therapy for hematological cancers, but resistance is a challenge that needs to be overcome. Through genetic, epigenetic and transcriptomic analyses, we found that venetoclax-resistant cells acquired a TP53 mutation and showed an upregulation of the PI3K/Akt pathway. Inhibition of Akt and ERK with TIC-10 induced apoptosis in venetoclax-resistant cells. TIC-10 might be a potential treatment option for venetoclax-resistant patients.
Article
Biochemistry & Molecular Biology
Anna Katrina Jogvansdottir Gradel, Stephanie K. K. Holm, Sarah Byberg, Myrte Merkestein, Wouter Frederik Johan Hogendorf, Mari Lilith Lund, Jesse Arnold Buijink, Jesper Damgaard, Jens Lykkesfeldt, Birgitte Holst
Summary: Ghrelin is a key hormone regulating energy balance, while LEAP2 acts as an endogenous antagonist of the GHSR. This study found that different meal challenges and diets can regulate the expression and secretion of LEAP2 in the small intestine and liver.
Article
Biochemistry & Molecular Biology
Emil List Larsen, Andreas Andersen, Laura K. Kjaer, Mie K. Eickhoff, Marie Frimodt-Moller, Frederik Persson, Peter Rossing, Jens Lykkesfeldt, Filip K. Knop, Tina Vilsboll, Jorgen Rungby, Henrik E. Poulsen
Summary: Animal studies have shown that SGLT2 inhibition decreases oxidative stress, which may explain the cardiovascular protective effects observed following SGLT2 inhibition treatment. This study investigated the effects of two and twelve weeks of SGLT2 inhibition on DNA and RNA oxidation, and found that longer-term dapagliflozin treatment decreased DNA oxidation.
FREE RADICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tina Ronn, Alexander Perfilyev, Josefine Jonsson, Karl-Fredrik Eriksson, Sine W. Jorgensen, Charlotte Brons, Linn Gillberg, Allan Vaag, Elisabet Stener-Victorin, Charlotte Ling
Summary: This study found an association between DNA methylation levels in adipose tissue and circulating lipid levels, and identified novel epigenetic changes associated with lipid levels. These findings provide insights into the relationship between dysregulation of circulating lipids and epigenetic mechanisms in adipose tissue, and have important implications for the study of metabolic syndrome.
HUMAN MOLECULAR GENETICS
(2023)
Article
Nutrition & Dietetics
Anitra C. Carr, Jens Lykkesfeldt
Summary: The study examines the relationship between vitamin C dose and concentration, specifically in relation to age and smoking status. It found that while vitamin C intake was similar across age groups, older individuals had significantly lower circulating vitamin C concentrations. This effect was more pronounced in non-smokers and suggests that older individuals may be more sensitive to low vitamin C intake, possibly due to the effects of long-term smoking and increased chronic disease prevalence.
Article
Nutrition & Dietetics
Kamilla Pedersen, David Hojland Ipsen, Josephine Skat-Rordam, Jens Lykkesfeldt, Pernille Tveden-Nyborg
Summary: This study investigated the effects of high-fat diets based on coconut oil or cocoa butter on glucose homeostasis and NASH in guinea pigs. The results showed that a diet high in long-chain fatty acids (LCFA) promoted metabolic imbalance and accelerated NASH-associated hepatic fibrosis. The study highlights the importance of evaluating fatty acid composition when investigating NASH-related endpoints.
Article
Nutrition & Dietetics
Anitra C. Carr, Jens Lykkesfeldt
Summary: In this study, the relationship between vitamin C status and demographic and lifestyle factors was investigated using NHANES 2017-2018 datasets. It was found that gender, smoking, and body weight have significant impacts on vitamin C requirements. Males and smokers require higher vitamin C intakes to achieve adequate serum concentrations. Overall, the general population should consume around 110 mg/day of vitamin C, smokers require approximately 165 mg/day, and heavier individuals (100+ kg) require about 155 mg/day to reach comparable vitamin C concentrations.
Article
Oncology
Katja Kaastrup, Linn Gillberg, Stine U. Mikkelsen, Andreas D. orskov, Claudia Schollkopf, Bo K. Mortensen, Bo Porse, Jakob W. Hansen, Kirsten Gronbaek
Summary: Idiopathic non-clonal cytopenia (ICUS) and clonal cytopenia (CCUS) are common in the elderly population. Aberrant DNA methylation, including hypermethylation of the LEP promoter, is associated with these disorders and myeloid neoplasms such as myelodysplastic syndrome (MDS). Hypermethylation of the LEP promoter is an early event in myeloid neoplasms and is associated with a worse prognosis.
CLINICAL EPIGENETICS
(2023)
Article
Cell Biology
Kirsten L. Wahlstrom, Hannah F. Hansen, Madeline Kvist, Jakob Burcharth, Jens Lykkesfeldt, Ismail Gogenur, Sarah Ekeloef
Summary: Endothelial dysfunction occurs due to inflammation and excessive reactive oxygen species production during surgical stress. Remote ischemic preconditioning (RIPC) may have anti-oxidative and anti-inflammatory properties, but it did not affect endothelial function or markers of nitric oxide (NO) bioavailability until 24 hours after sub-acute laparoscopic cholecystectomy. Surgical removal of the inflamed gallbladder reduced markers of NO bioavailability and oxidative stress caused by acute cholecystitis.
Editorial Material
Physiology
Arthur Ingersen, Casper Soendenbroe, Haboon Ismail Ahmed, Jacob Borch, Kristine Frosig Moseholm, Mette Hyldig Dal, Olsi Kusta, Sofie Olsgaard Bergien, Linn Gillberg
Article
Hematology
Jesper Therkelsen, Dicte Wilhjelm Traeden, Ida Schjodt, Mette Klarskov Andersen, Lene Dissing Sjo, Jakob Werner Hansen, Kirsten Gronbaek, Konstantinos Dimopoulos
Summary: A novel score, ProGraME, combining flow cytometry (FC), morphology, and cytogenetic/molecular assessment, is developed for the diagnosis of low-risk myelodysplasia without minimal requirements of flow analysis other than accurate population gating.
EUROPEAN JOURNAL OF HAEMATOLOGY
(2023)
Article
Genetics & Heredity
Marwa Almosailleakh, Sofia Bentivegna, Samuele Narcisi, Sebasitien J. Benquet, Linn Gillberg, Carmen P. Montano-Almendras, Simonas Savickas, Erwin M. Schoof, Amelie Wegener, Herve Luche, Henrik E. Jensen, Christophe Come, Kirsten Gronbaek
Summary: KANK1 is a proposed tumour suppressor gene that is reduced or absent in several types of tumour tissue. A new germline loss of heterozygosity mutation in the KANK1 gene was found in a patient diagnosed with myelodysplastic neoplasm. KANK1 knockout mice showed altered colony forming and proliferative potential of bone marrow cells, as well as a decrease in hematopoietic stem and progenitor cells.