Article
Polymer Science
Monic Andrew, Gurunathan Jayaraman
Summary: The study investigates the structure and biocompatibility of EPS produced by Virgibacillus dokdonensis VITP14, showing its porous web-like structure, anionic nature, and potential application in synthesizing biomaterials.
Article
Microbiology
Jiading Liu, Xinyu Zhang, Hao Cao, Liwei Guo, Baisuo Zhao, Xiaoxia Zhang, Yanan Wang, Haisheng Wang
Summary: A new species of Alteribacter, named Alteribacter keqinensis sp. nov., was isolated from a soda lake and identified using a polyphasic taxonomic approach. This bacterium displayed a wide range of growth conditions and possessed characteristic features of the genus Alteribacter.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Geon-Yeong Cho, Kyung- Sook Whang
Summary: In this study, a novel moderately halophilic bacterium was isolated from a grey solar saltern in Shinan, Republic of Korea. The bacterium, named Aquibacillus saliphilus sp. nov., is rod-shaped and facultative anaerobic. It uses nitrates for anaerobic respiration and does not produce glucose acids. The bacterium belongs to the Aquibacillus genus and shows high similarity to Aquibacillus halophilus B6B(T) in its 16S rRNA gene sequence.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rebekka H. Luelf, Maik Hilgarth, Matthias A. Ehrmann
Summary: Three moderately halophilic strains were isolated from a lupine-based moromi fermentation and identified as a novel species of the genus Chromohalobacter. These strains exhibited the ability to grow under various salt concentrations and temperatures, and their genome characteristics differed from other closely related strains within the genus.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Yun Wang, Gang-Qiang Jiang, Hong-Ping Lin, Peng Sun, Hong-Yan Zhang, Dong-Mei Lu, Li-Yun Wang, Chang-Jin Kim, Shu-Kun Tang
Summary: The bacterial strain YIM 93176(T) isolated from a saltern in Korea is a novel species belonging to the genus Lentibacillus, named Lentibacillus saliphilus sp. nov., based on polyphasic taxonomic analysis. Genomic sequencing revealed important characteristics such as genome size, fatty acids composition, and respiratory quinone, supporting its affiliation to the Lentibacillus genus.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Kuppusamy Pandiyan, Prity Kushwaha, Samadhan Y. Bagul, Hillol Chakdar, Munusamy Madhaiyan, Srinivasan Krishnamurthi, Pravin Kumar, Nanjappan Karthikeyan, Arjun Singh, Murugan Kumar, Udai Bhan Singh, Anil Kumar Saxena
Summary: Strain D1-1(T) is a moderately halophilic, Gram-stain-negative, aerobic bacterium belonging to the genus Halomonas, closely related to Halomonas alimentaria YKJ-16(T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Ya-Lin Yin, Fang-Ling Li, Lei Wang
Summary: A bacterium named G5-11(T) was isolated from saline soil in Yingkou, Liaoning, China. It is a Gram-negative, moderately halotolerant, facultatively aerobic, and motile bacterium. Phenotypic, chemotaxonomic, and phylogenetic analyses showed that strain G5-11(T) represents a novel species of the genus Halomonas, named Halomonas salinarum.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Lingmin Jiang, Won Yong Jung, Zhun Li, Mi-Kyung Lee, Seung-Hwan Park, Se Won Kang, Jung-Sook Lee, Hyunjung Jung, Tai-Young Hur, Hyeun Bum Kim, Jae-Kyung Kim, Ju-Hoon Lee, Ju Huck Lee, Jiyoung Lee
Summary: AGMB 02131(T) is a novel Gram-positive, facultatively anaerobic, rod-shaped strain isolated from a cow faecal sample, belonging to the genus Peribacillus. Based on phylogenetic, genomic, and chemotaxonomic features, it has been proposed as a new species named Peribacillus faecalis sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Tong-Tong Zhang, Dun Liu, Xiao-Yu Zhang, Jia-Cheng Wang, Zong-Jun Du
Summary: A moderately halophilic bacterium, designated C176(T), was isolated from Yuncheng Salt Lake in China. Phylogenetic analysis showed that strain C176(T) is closely related to Spiribacter salinus LMG 27464(T). The major fatty acids of strain C176(T) are Summed feature 8 and C-16:0, and the predominant ubiquinone is Q-8. Assessment of polyphasic taxonomy confirms strain C176(T) as a novel species of the genus Spiribacter, named Spiribacter salilacus sp. nov.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Microbiology
Xu Qiu, Xiaorong Cao, Guangxin Xu, Huangming Wu, Xixiang Tang
Summary: A novel halophilic bacterium, strain QX-1( T), was isolated from deep-sea sediment in the southwestern Indian Ocean. It belongs to the genus Halomonas and shows close phylogenetic relationship to other species. The novel species, named Halomonas maris, exhibits unique morphological, physiological, and chemical properties, as supported by phylogenetic, phenotypic, and chemotaxonomic analyses.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Auttaporn Booncharoen, Wonnop Visessanguan, Nattakorn Kuncharoen, Supalurk Yiamsombut, Pannita Santiyanont, Wuttichai Mhuantong, Pornchai Rojsitthisak, Somboon Tanasupawat
Summary: The strain SKP4-6(T) is a rod-shaped, endospore-forming, moderately halophilic bacterium belonging to the genus Halobacillus. It has been identified as a novel species named Halobacillus fulvus sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Fatmah O. Sefrji, Gregoire Michoud, Ramona Marasco, Giuseppe Merlino, Daniele Daffonchio
Summary: The bacterium strain R1DC9(T) isolated from sediments of a mangrove stand on the Red Sea coast of Saudi Arabia is a distinct species within the phylum Bacteroidetes, representing a new genus in the order Cytophagales. It shows unique characteristics compared to known relatives and is proposed to belong to a new family Mangrovivirgaceae fam. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Shan-shan Xu, Qi-liang Lai, Zeng-zhi Liu, Ying Xu
Summary: A novel moderately halophilic bacterial strain, designated Z330(T), was isolated from the egg of a marine invertebrate of the genus Onchidium collected in the South China Sea. The strain showed high similarity to Paracoccus fistulariae KCTC 22803(T), Paracoccus seriniphilus NBRC 100798(T), and Paracoccus aestuarii DSM 19484(T). It grew optimally at specific temperature, pH, and NaCl concentration, indicating that it is a moderately halophilic and halotolerant bacterium of genus Paracoccus.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
Yu-Hui Wang, Jun-Cheng Liu, Yi-Heng Du, Jin-Hao Xu, Zong-Jun Du, Meng-Qi Ye
Summary: This study compares and analyzes the properties of seven species from three genera of the Roseobacteraceae family, including phylogenetic, genomic, biochemical, and chemo taxonomical properties. A novel strain named C21-152(T) is isolated from solar saltern sediment in Weihai, China, and its similarities to Psychromarinibacter halotolerans MCCC 1K03203(T) are determined. Strain C21-152(T) shows resistance to antibiotics and the ability to survive in low salinity environments.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Infectious Diseases
S. Konate, A. Camara, C. Lo, M. Tidjani Alou, A. Hamidou Togo, S. Niare, N. Armstrong, A. Djimde, M. A. Thera, F. Fenollar, D. Raoult, M. Million
Summary: A moderately halophilic and strictly aerobic bacterium named Virgibacillus doumboii sp. nov. was isolated from a human stool as part of a study on the diagnosis of childhood malnutrition in Mali. It shares the highest ORTHOANI value with Virgibacillus kekensis, suggesting a close phylogenetic relationship within the Virgibacillus genus.
NEW MICROBES AND NEW INFECTIONS
(2021)
Article
Biochemistry & Molecular Biology
Amel Dahdouh, Nawel Boucherba, Khelifa Bouacem, Sondes Mechri, Adel Amirouche, Ali Aksas, Bassem Jaouadi, Djamel Edine Kati
Summary: The study purified a plant peroxidase TRP from turnip roots and characterized its biochemical properties, revealing high catalytic efficiency and antioxidant activities, indicating its potential application in enzyme therapy for the pharmaceutical and medicinal industries.
BIOCATALYSIS AND BIOTRANSFORMATION
(2022)
Article
Biochemistry & Molecular Biology
Khelifa Bouacem, Fawzi Allala, Nadia Zarai Jaouadi, Sondes Hamdi, Sondes Mechri, Kahina Ighilahriz, Hatem Rekik, Hocine Hacene, Amel Bouanane-Darenfed, Bassem Jaouadi
Summary: A novel extracellular lignin peroxidase (LiP PR40) was produced by a white-rot Agaricomycetes fungus strain newly isolated from dead deciduous trees in Algeria. This purified enzyme showed premium activity at pH 3 and 80 degrees C, and exhibited higher catalytic efficiency and dye-decolorisation aptitude compared to commercial horseradish peroxidase and reported LiP BA45. These properties make LiP PR40 a potential candidate for destaining synthetic dyes and lignin biodegradation.
BIOCATALYSIS AND BIOTRANSFORMATION
(2022)
Article
Biochemistry & Molecular Biology
Sondes Mechri, Nadia Zarai Jaouadi, Khelifa Bouacem, Fawzi Allala, Aicha Bouraoui, Celine Ferard, Hatem Rekik, Alexandre Noiriel, Abdelkarim Abousalham, Amel Bouanane-Darenfed, Hocine Hacene, Florence Lederer, Laura Baciou, Bassem Jaouadi
Summary: In this study, the sapN gene encoding subtilisin SAPN from Melghiribacillus thermohalophilus Nari2AT was successfully isolated, cloned, and expressed in both P. pastoris and E. coli, with His6-tagged enzymes showing the highest activity and stability. Comparative analysis revealed that these enzymes have great potential for industrial applications due to their catalytic efficiency and hydrolysis degree.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Sondes Mechri, Khelifa Bouacem, Taha-Bilel Chalbi, Marwa Khaled, Fawzi Allala, Amel Bouanane-Darenfed, Hocine Hacene, Bassem Jaouadi
Summary: This study focuses on the protease production and characteristics of a strain called TN-X30, identified as Streptomyces mutabilis. The researchers found that optimizing the culture conditions and using lyophilization techniques improved the protease yield and stability. The TN-X30 protease showed excellent performance in detergent formulations, reducing potential environmental chemical threats.
JOURNAL OF SURFACTANTS AND DETERGENTS
(2022)
Article
Ecology
Sidali Kourdali, Kamel Boudjema, Abdellah Meknachi, Nabila Bounakous, Bassem Jaouadi, Sondes Mechri, Abdelmalek Badis
Summary: The present research aims to evaluate the variation of several responses in marine mussels exposed to different sub-lethal concentrations of copper and lead. The results showed that temperature and salinity variations were not significant enough to interfere with the effect of the pollutants. Changes in nitrogen and phosphorus mineral matter were found to be a physiological disturbance in both species. During the contamination cycles, catalase induction and protein depletion reflected the oxidative stress caused by the metals. However, the catalase activity returned to normal state during the decontamination cycles.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ismail Hasnaoui, Ahlem Dab, Sondes Mechri, Houssam Abouloifa, Ennouamane Saalaoui, Bassem Jaouadi, Alexandre Noiriel, Abdeslam Asehraou, Abdelkarim Abousalham
Summary: A novel extracellular lipase P22 from Penicillium crustosum Thom was isolated and characterized. The specific activities of P22 lipase were found to be 5000 and 10,000 U/mg on olive oil and trioctanoin emulsions, respectively. It exhibited clear regioselectivity toward the sn-1 position of surface-coated triglycerides and showed high activity and stability in the presence of water-immiscible organic solvents, surfactants, and oxidizing agents. It also showed compatibility with commercial laundry detergents and effectively removed oil stains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sondes Hamdi, Fawzi Allala, Sondes Mechri, Khelifa Bouacem, Hatem Rekik, Hocine Hacene, Bassem Jaouadi, Marilize Le Roes-Hill, Nadia Zarai Jaouadi
Summary: Textile dyes in wastewater from textile factories pose a significant environmental problem, threatening aquatic life. In this study, a novel enzyme called MnP AN30 was found to effectively decolorize various textile dyes with high efficiency. The gene encoding this enzyme was cloned and expressed, and the recombinant enzyme showed similar biochemical properties to the native enzyme. This research provides a potential candidate for the biological treatment and decolorization of synthetic textile dyes.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Sondes Mechri, Fawzi Allala, Khelifa Bouacem, Ismail Hasnaoui, Hassan Gwaithan, Taha Bilel Chalbi, Ennouamane Saalaoui, Abdeslam Asehraou, Alexandre Noiriel, Abdelkarim Abousalham, Hocine Hacene, Amel Bouanane-Darenfed, Marilize Le Roes-Hill, Bassem Jaouadi
Summary: In this study, a serine alkaline protease named SPSM was purified and characterized from Streptomyces mutabilis strain TN-X30. The SPSM gene was cloned and over-expressed in E. coli BL21(DE3)pLysS and E. coli BL21-AITM strains. The immobilization of SPSM using encapsulation and adsorption-encapsulation approaches and different carriers was investigated. The results showed that the immobilized SPSM exhibited similar characteristics to the soluble form, and the white clay and kaolin used as binders showed great potential in the immobilization of SPSM.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Boutheina Bessadok, Bassem Jaouadi, Thomas Brueck, Andrea Santulli, Concetta Maria Messina, Saloua Sadok
Summary: This study isolated and characterized five strains of marine yeast, which showed high protein content, essential amino acids, and polyunsaturated fatty acids. They also exhibited good cell viability and safety. Based on their composition and literature investigation, these yeasts could be classified as oleaginous yeasts, single cell protein (SCP), and single cell oil (SCO) producers, providing alternative ingredients for various sectors including food and feed.
FERMENTATION-BASEL
(2022)
Article
Horticulture
Adiba Benahmed Djilali, Rebiha Mehraz, Khelifa Bouacem, Abdelouahb Benseddik, Idir Moualek, Mohamed Nabiev, Abdelmadjid Benzara
Summary: The study evaluated the phytochemical, antimicrobial, and insecticidal properties of tannins derived from Cydonia oblonga fruit, showing significant extraction levels using different solvents and salts. The extracted tannins had no antimicrobial activity against the tested strains, but demonstrated a bio-insecticidal effect on Tribolium confusum adults. The findings suggest that Cydonia oblonga fruit could be a potential alternative for the development of natural drugs and insecticides against parasites.
INTERNATIONAL JOURNAL OF FRUIT SCIENCE
(2021)
Article
Environmental Sciences
Mouna Ben Elhoul, Nadia Zarai Jaouadi, Khelifa Bouacem, Fawzi Allala, Hatem Rekik, Sondes Mechri, Haifa Khemir Ezzine, Neila Miled, Bassem Jaouadi
Summary: The keratin-degrading bacterium Actinomadura viridilutea DZ50 secretes a keratinase (KERDZ) with potential industrial interest. Recombinant enzyme rKERDZ has been shown to completely degrade chicken feather and remove wool/hair from various hides, while preserving collagen structure. The enzymatic process reduces biological and chemical oxygen demands in leather processing and offers a greener alternative to traditional dehairing methods.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
