期刊
BIOPHYSICAL JOURNAL
卷 108, 期 11, 页码 2680-2690出版社
CELL PRESS
DOI: 10.1016/j.bpj.2015.04.027
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资金
- Ministry of Education, Science, and Culture of Japan
Halorhodopsin from Natronomonas pharaonis (pHR) functions as a light-driven halide ion pump. In the presence of halide ions, the photochemical reaction of pHR is described by the scheme: K -> L-1 -> L-2 -> N -> O -> pHR' -> pHR. Here, we report light-induced structural changes of the pHR-bromide complex observed in the C2 crystal. In the L-1-to-L-2 transition, the bromide ion that initially exists in the extracellular vicinity of retinal moves across the retinal Schiff base. Upon the formation of the N state with a bromide ion bound to the cytoplasmic vicinity of the retinal Schiff base, the cytoplasmic half of helix F moves outward to create a water channel in the cytoplasmic interhelical space, whereas the extracellular half of helix C moves inward. During the transition from N to an N-like reaction state with retinal assuming the 13-cis/15-syn configuration, the translocated bromide ion is released into the cytoplasmic medium. Subsequently, helix F relaxes into its original conformation, generating the O state. Anion uptake from the extracellular side occurs when helix C relaxes into its original conformation. These structural data provide insight into the structural basis of unidirectional anion transport.
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