Article
Agriculture, Dairy & Animal Science
Md Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
Summary: This study identified 2225 proteins in small extracellular vesicles from late-stage lactating cows, with 429 of them being newly identified when compared with previous datasets. The newly identified proteins are suggested to play a role in various molecular phenomena relevant to mammary gland physiology, milk production, immunity, and immune response during late-stage lactation, expanding the understanding of the molecular cargos, nutritional status, and immune modulation of small extracellular vesicles in milk.
Article
Biochemistry & Molecular Biology
Magdalena Surman, Sylwia Kedracka-Krok, Urszula Jankowska, Anna Drozdz, Ewa Stepien, Malgorzata Przybylo
Summary: The study focused on identifying biologically relevant proteins in UBC-derived ectosomes, showing that T-24 ectosomes contained more unique proteins and promoted proliferation and motility of recipient cells by transferring cancer-promoting molecules. The findings confirm the role of UBC-derived ectosomes in cancer development and progression, suggesting their potential as diagnostic and prognostic biomarkers in bladder cancer management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Runsheng Zheng, Natalia Govorukhina, Tabiwang N. Arrey, Christopher Pynn, Ate van der Zee, Gyorgy Marko-Varga, Rainer Bischoff, Alexander Boychenko
Summary: This study compares 1D-nanoLC-MS with an automated online-2D high-pH RP x low pH RP separation method for deep proteome profiling, demonstrating that the new method can increase peptide and protein identifications without the need for extra sample handling. Serum sample preparation related changes were revealed, which should be taken into account when planning multicentric biomarker discovery and validation studies.
ANALYTICAL CHEMISTRY
(2021)
Article
Fisheries
Sarah L. Carroll, Vernon E. Coyne
Summary: Ocean acidification is becoming more pervasive due to increasing CO2 emissions and global climate change, affecting various trophic levels and posing a threat to community structures and the sustainability of the fishing/aquaculture industry. Understanding the molecular stress response of key organisms can help predict their survival under changing environmental conditions. This study focused on elucidating the molecular stress response of the South African abalone using high throughput iTRAQ-based proteomics methodology.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Food Science & Technology
Edilza Silva do Nascimento, Katya Anaya, Julia Mariano Caju de Oliveira, Jose Thalles Jocelino Gomes de Lacerda, Michael Edward Miller, Meriellen Dias, Maria Anita Mendes, Juliana de Azevedo Lima Pallone, Clarice Weis Arns, Maria Aparecida Juliano, Tatiane Santi Gadelha, Maria Teresa Bertoldo Pacheco, Carlos Alberto de Almeida Gadelha
Summary: This study identified bioactive peptides generated by in vitro gastrointestinal digestion of yam proteins. The peptides showed antioxidant, DNA damage protection, ACE-inhibitory, and antibacterial activities. The total gastrointestinal phase hydrolysate displayed stronger DNA damage protection and antibacterial activity against Escherichia coli.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Environmental Sciences
Marilina Fernandez, Eduardo A. Callegari, Maria D. Paez, Paola S. Gonz, Elizabeth Agostini
Summary: This study utilized shotgun proteomic technology combined with Gel-LC to investigate the intracellular protein profile of Acinetobacter guillouiae SFC 500-1 A when grown in tannery wastewater. It was found that certain proteins involved in aromatic compound degradation and siderophore biosynthesis were specifically expressed in the wastewater treatment. Additionally, 1598 proteins showed differential expression between wastewater and normal culture conditions. Upregulated proteins in the wastewater treatment were associated with the mentioned processes, oxidative stress mitigation, and intracellular redox state regulation. Interestingly, downregulated proteins under the same treatment were mainly linked to the regulation of basic cellular functions, metabolism, and other complex responses. These findings suggest that SFC 500-1 A employs survival and stress management strategies to cope with the toxic effects of tannery wastewater, primarily focused on minimizing metabolic processes. The results could be valuable for enhancing the strain's efficacy in future bioremediation applications.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Food Science & Technology
Ana G. Abril, Monica Carrera, Karola Boehme, Jorge Barros-Velazquez, Benito Canas, Jose-Luis R. Rama, Tomas G. Villa, Pilar Calo-Mata
Summary: This study utilized LC-ESI-MS/MS MS to analyze peptides from bacteriophages infecting mastitis-causing Staphylococcus aureus, identifying a large number of specific peptides that could be useful for identifying and characterizing the bacteria causing mastitis. Additionally, a study of bacteriophage phylogeny and its relationship with the respective Staphylococcus spp. infected was conducted, revealing specific peptides present in closely related phages and highlighting the connection between bacteriophage phylogeny and the infected Staphylococcus spp.
Article
Biochemistry & Molecular Biology
Eser Unsaldi, Aslihan Kurt-Kizildogan, Servet Ozcan, Dorte Becher, Birgit Voigt, Caner Aktas, Gulay Ozcengiz
Summary: This study investigated the complex metabolic changes in Streptomyces clavuligerus AK39 resulting from disruption of the hom gene, identifying differentially expressed proteins associated with secondary metabolism and unknown functions. Key findings included increased levels of positive regulator CcaR and enzyme CeaS2 in the cephamycin C biosynthetic pathway. These results provide potential targets for improving cephamycin C production in Streptomyces clavuligerus.
PROTEIN AND PEPTIDE LETTERS
(2021)
Article
Food Science & Technology
Yung-Husan Chen, Yu-Wei Chang, Chu-Wen Ma, Lian-Zhong Luo, Ting-Jang Lu, Jeng-Yuan Yao
Summary: This study analyzed the bioactive compounds of cultured three-spot seahorse using HPLC-ESI/MS/MS and evaluated their biological activities. Taurine and arginine were identified as the primary bioactive compounds, with the water layer extract containing the highest content. Additionally, the water layer extract demonstrated anti-inflammatory activity.
FOOD SCIENCE & NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Ana G. Abril, Monica Carrera, Karola Bohme, Jorge Barros-Velazquez, Pilar Calo-Mata, Angeles Sanchez-Perez, Tomas G. Villa
Summary: The study utilized LC-ESI-MS/MS for a comprehensive shotgun proteomics characterization of pathogenic Listeria species, identifying numerous important proteins involved in Listeria pathogenicity. This proteomic repository can serve as a foundation for further research on pathogenic Listeria species and the development of novel therapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Stavros C. Proikakis, Efterpi V. Bouroutzika, Athanasios K. Anagnostopoulos, George Th. Tsangaris
Summary: The study investigated the proteomic content of donkey's milk and identified 633 different proteins, categorized based on their molecular function and biological process. The results were visualized graphically using the GeneOntology (GO) system, confirming the high nutritional value of donkey milk and potential for future optimization.
Article
Biochemical Research Methods
Huichun Shi, Siyuan Liu, Zhimi Tan, Lin Yin, Liyan Zeng, Tiefu Liu, Shuye Zhang, Lijun Zhang
Summary: This study revealed the cellular mechanisms of EV71 infection and identified the glycolysis pathway as a key player. ENO1 was identified as a potential target against EV71, and DCA could act as an inhibitor.
Article
Ecology
Purnima Singh, Sakae Tsuda, Shiv Mohan Singh, Sukanta Mondal, Utpal Roy
Summary: Antifreeze proteins (AFPs) play crucial roles in the survival of microorganisms in cold environments. This study characterized a cry-c AFP from an Arctic glacier bacterial strain, revealing its molecular mass, amino acid residues, and structure-function correlation in terms of its antifreeze properties.
Article
Immunology
Jae Won Seo, Kyu Seong Park, Gwang Bin Lee, Sang-eun Park, Jae-Hoon Choi, Myeong Hee Moon
Summary: Lipid accumulation in macrophages is a prominent phenomenon in atherosclerosis. Lipid profiling of intimal macrophages is important for understanding the phenotypic changes of macrophages in atherosclerotic lesions.
Article
Food Science & Technology
Worawat Surarit, Nitra Nuengchamnong, Pintusorn Hansakul
Summary: The rhizome ethanolic extract of Dioscorea birmanica Prain & Burkill (DBE) has potent antioxidant activities, including moderate metal chelating activity. Additionally, it improved cell viability, attenuated oxidative damage, and recovered the activities of antioxidant enzymes against sodium nitroprusside (SNP)-induced oxidative stress in normal hepatic cells. The protective mechanism of DBE involves significant reduction of excess levels of intracellular free radicals.
Article
Physiology
Phil Jun Lee, Namki Cho, Hee Min Yoo, Hong Pyo Kim
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Dae Kil Jang, Ik-Soo Lee, Han-Seung Shin, Hee Min Yoo
Article
Endocrinology & Metabolism
Sung B. Park, Byumseok Koh, Won H. Jung, Kyoung J. Choi, Yoon J. Na, Hee M. Yoo, Sunray Lee, Dukjin Kang, Dong-Mok Lee, Ki Y. Kim
DIABETES OBESITY & METABOLISM
(2020)
Article
Gastroenterology & Hepatology
Yong Moon Lee, Sun Hyung Kang, Ju Seok Kim, Hyuk Soo Eun, Jong Seok Joo, Woo Sun Rou, Jae Ho Park, Hee Seok Moon, Eaum Seok Lee, Seok Hyun Kim, Jae Kyu Sung, Byung Seok Lee, Hyun Yong Jeong, Min Kyung Yeo, Kyu Sang Song, Hee Min Yoo
DIGESTIVE DISEASES
(2020)
Article
Biotechnology & Applied Microbiology
Changwoo Park, Jina Lee, Zohaib Ul Hassan, Keun Bon Ku, Seong-Jun Kim, Hong Gi Kim, Edmond Changkyun Park, Gun-Soo Park, Daeui Park, Seung-Hwa Baek, Dongju Park, Jihye Lee, Sangeun Jeon, Seungtaek Kim, Chang-Seop Lee, Hee Min Yoo, Seil Kim
Summary: The study found that ddPCR is more sensitive than RT-qPCR in detecting viral RNA, especially for determining the copy number of reference materials.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Phil Jun Lee, Chau Ha Pham, Nguyen Thi Thanh Thuy, Hye-Jin Park, Sung Hoon Lee, Hee Min Yoo, Namki Cho
Summary: The study revealed that MLG attenuates glutamate-induced apoptosis in HT22 cells by inhibiting intracellular ROS generation and mitochondrial dysfunction, as well as by modulating the expression of key proteins involved in apoptotic pathways. Additionally, MLG treatment induced HO-1 expression in HT22 cells, suggesting a potential mechanism for its neuroprotective effects against glutamate-induced neurotoxicity.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joo-Eun Lee, Fang Bo, Nguyen Thi Thanh Thuy, Jaewoo Hong, Ji Shin Lee, Namki Cho, Hee Min Yoo
Summary: The study demonstrated that lesbicoumestan can induce mitochondrial depolarization on Jurkat cells through MALT1 degradation, inhibiting the NF-kappa B pathway, and exhibiting potential anticancer effects against human leukemia cancer cells.
Article
Biochemistry & Molecular Biology
Hansol Joo, Mi-Kyung Oh, Ji Yeon Kang, Hyun Sung Park, Dong-Hoon Chae, Jieun Kim, Jong-Hee Lee, Hee Min Yoo, Uimook Choi, Do-Kyun Kim, Hakmo Lee, Sungjoo Kim, Kyung-Rok Yu
Summary: This study demonstrates that EVs secreted from WJ-MSCs primed with ER stress inducer TSG have enhanced immunomodulatory properties, showing potential for treating immune-mediated inflammatory diseases.
Review
Biochemistry & Molecular Biology
Hee Min Yoo, Il-Hwan Kim, Seil Kim
Summary: This review discusses the importance of accuracy and sensitivity in COVID-19 diagnosis, highlighting various detection methods such as PCR, digital PCR, and RT-LAMP. Non-conventional molecular diagnostic methods like NGS, CRISPR technology, and nanotechnology are also improving the accuracy of COVID-19 testing. Additionally, the standardization of SARS-CoV-2 nucleic acid testing and the use of reference materials as resources are emphasized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Sung Rae Kim, Yongun Park, Mo Li, Yeong Kyeong Kim, Sunmin Lee, Su Young Son, Sarah Lee, Jong Seok Lee, Choong Hwan Lee, Hyun Ho Park, Ji-Yun Lee, Sungguan Hong, Young-Chang Cho, Jung-Woong Kim, Hee Min Yoo, Namki Cho, Hyun-Shik Lee, Sung Hoon Lee
Summary: The ethanol extract of Ailanthus altissima leaves (AAE) inhibited inflammation in astrocytes by reducing the expression of iNOS and COX-2, suppressing the activation of NF-kappa B, ERK, and JNK, and reducing nitrite levels. AAE also attenuated LPS-induced memory and social impairment in mice and suppressed ERK and JNK activation in the cortices of mice.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chau Ha Pham, Joo-Eun Lee, Jinha Yu, Sung Hoon Lee, Kyung-Rok Yu, Jaewoo Hong, Namki Cho, Seil Kim, Dukjin Kang, Soojin Lee, Hee Min Yoo
Summary: Recent studies have shown that propionic acid inhibits the survival of HeLa cells through inducing autophagy, as well as triggering the production of reactive oxygen species (ROS), mitochondrial membrane dysfunction, and affecting the NF-κB and AKT/mTOR signaling pathways.
Article
Biochemistry & Molecular Biology
Bo Fang, Zijun Li, Yinda Qiu, Namki Cho, Hee Min Yoo
Summary: Breast cancer is the most common type of cancer worldwide, with a lack of effective therapies. Usenamine A, a natural compound, has shown promising anti-proliferative effects, cell cycle arrest, and induction of autophagy in breast cancer cells.
Article
Biochemical Research Methods
Sang-Soo Lee, Seil Kim, Hee Min Yoo, Da-Hye Lee, Young-Kyung Bae
Summary: This study reported the production of a lentiviral SARS-CoV-2 reference material, which can be used to evaluate the quality and performance of SARS-CoV-2 molecular testing, providing accurate copy number values to improve the reliability of SARS-CoV-2 molecular testing.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Bo Fang, Guijae Yoo, Phil Jun Lee, Yinda Qiu, Sung Hoon Lee, Ji Shin Lee, Hee Min Yoo, Namki Cho
Summary: Using a network pharmacology approach, this study identified potential targets and verified the anti-breast cancer activity of SSD. PPAR gamma was identified as a potential therapeutic target for SSD in breast cancer treatment.
NATURAL PRODUCT COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Hyun Sung Park, Mi-Kyung Oh, Joong Won Lee, Dong-Hoon Chae, Hansol Joo, Ji Yeon Kang, Hye Bin An, Aaron Yu, Jae Han Park, Hee Min Yoo, Hyun Jun Jung, Uimook Choi, Ji-Won Jung, In-Sook Kim, Il-Hoan Oh, Kyung-Rok Yu
Summary: Exposure to DEP enhances pro-inflammatory cytokines and immune responses through the ROS/ERK/cFos pathway in WJ-MSCs, impairing their therapeutic effect in colitis. Modulating ROS/ERK/cFos signaling pathways in WJ-MSCs may be a novel therapeutic strategy for DEP-induced diseases.
INTERNATIONAL JOURNAL OF STEM CELLS
(2022)