Article
Plant Sciences
Danila Cabral, Helkin Forero Ballesteros, Bruno Paes de Melo, Isabela Tristan Lourenco-Tessutti, Kercya Maria Simoes de Siqueira, Luciana Obicci, Maria Fatima Grossi-de-Sa, Adriana S. Hemerly, Janice de Almeida Engler
Summary: Research on the role of ABAP1 in root-knot nematode-induced galls shows that it can affect gall size and nematode growth and reproduction, participating in cell cycle regulation and preventing further expansion of galls.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Savio D. Rodrigues, Mansour Karimi, Lennert Impens, Els Van Lerberge, Griet Coussens, Stijn Aesaert, Debbie Rombaut, Dominique Holtappels, Heba M. M. Ibrahim, Marc Van Montagu, Jeroen Wagemans, Thomas B. Jacobs, Barbara De Coninck, Laurens Pauwels
Summary: Agrobacterium spp. are important plant pathogens that can be genetically modified using CRISPR-mediated base editing to introduce targeted point mutations, improving the efficiency and functionality of the strains for plant transformation and gene editing. The method developed in this study has shown high efficiency and potential for future advancements in engineering Agrobacterium strains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Huiqing Yan, Dandan Ma, Peipei Yi, Guilian Sun, Xingyan Chen, Yin Yi, Xiaolong Huang
Summary: In this study, we established a rapid and efficient hairy root transformation system in strawberry, which is suitable for promoter analysis and functional characterization of candidate genes in strawberry and other rosaceous plants.
Article
Plant Sciences
Lili Zhou, Yali Wang, Peilin Wang, Chunling Wang, Jiamin Wang, Xingfen Wang, Hongmei Cheng
Summary: This study optimized the Agrobacterium rhizogenes-mediated hairy root method and successfully detected CRISPR/Cas9-induced gene editing mutations in cotton. The findings provide a technical basis for functional genomics research of cotton.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu, Jietang Zhao
Summary: In this study, a rapid and efficient transformation system using A. rhizogenes strain MSU440 was established for gene function analysis in litchi hairy roots by optimizing parameters. The system was successfully used to study the function of the key anthocyanin regulatory gene LcMYB1 in litchi, showing higher contents of anthocyanins, proanthocyanins, and flavonols in the over-expressed LcMYB1 hairy roots.
Article
Biotechnology & Applied Microbiology
Pijush Paul, Sukanya Majumdar, Sumita Jha
Summary: The establishment of high transformation frequency hairy root culture of Arabidopsis thaliana was achieved in this study. Long term maintenance was successfully achieved in hormone free media. Optimization of culture medium and standardization of co-cultivation period played a crucial role in obtaining high frequency hairy roots.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Cleberson Ribeiro, Bruno Paes de Melo, Isabela Tristan Lourenco-Tessutti, Helkin Forero Ballesteros, Karla Veloso Goncalves Ribeiro, Killian Menuet, Jefri Heyman, Adriana Hemerly, Maria Fatima Grossi de Sa, Lieven De Veylder, Janice de Almeida Engler
Summary: The establishment of root-knot nematode-induced galls in plant host roots likely involves a wound-induced regeneration response. The ERF115-PAT1 complex plays a crucial role in wound healing and cell division, contributing to gall formation and development.
Article
Horticulture
Lisha Gong, Min Lu, Huaming An
Summary: This study demonstrates that Agrobacterium-mediated transformation is a simple, fast, and efficient approach for generating composite R. roxburghii plants.
Article
Plant Sciences
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao
Summary: This study developed a regeneration system for Chinese cabbage and established a simple and efficient genetic transformation method. The method can obtain transformants in a relatively short period of time, paving the way for further gene editing and functional studies.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Plant Sciences
Jaap-Jan Willig, Nina Guarneri, Joris J. M. van Steenbrugge, Willem de Jong, Jingrong Chen, Aska Goverse, Jose L. Lozano Torres, Mark G. Sterken, Jaap Bakker, Geert Smant
Summary: This study reveals that the transcription factor TCP9 modulates the root system architectural plasticity to nematode infections via ROS-mediated processes, mitigating the impact of belowground biotic stresses.
Article
Ecology
Md Maniruzzaman Sikder, Mette Vestergard, Tina Kyndt, Olivera Topalovic, Enoch Narh Kudjordjie, Mogens Nicolaisen
Summary: Understanding the effects of secondary metabolites on nematode communities can help develop novel nematode management strategies. In this study, genetically altered Arabidopsis thaliana plants were used to analyze the root-associated nematode communities and their interaction with associated microbiota. The results showed that certain metabolites had stronger effects on plant parasitic nematodes, and the microbiomes from these plants also influenced the invasion ability of the parasitic nematode Meloidogyne incognita. Flavonoids were identified as potential mediators of this microbiome-mediated effect.
Article
Multidisciplinary Sciences
Ines Modesto, Vera Inacio, Yves Van de Peer, Celia M. Miguel
Summary: This study investigated the role of small RNAs (sRNAs) in regulating the interaction between Pinus pinaster and pinewood nematodes (PWN). Several microRNAs (miRNAs) responsive to PWN were identified, and some of these miRNAs were associated with pathways related to jasmonate-response, ROS detoxification, and terpenoid biosynthesis. Furthermore, several miRNAs with putative functions in plant defense and resistance to PWN were identified by comparing resistant and susceptible plants. The study also explored the possibility of bidirectional trans-kingdom RNA silencing between P. pinaster and PWN.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Shilpi Sharma, Yeshveer Singh, Praveen K. Verma, Jyoti Vakhlu
Summary: Efficient transformation system for genetic improvement is crucial in Crocus sativus due to its lack of sexual reproduction. This study established a successful protocol for the transformation of Crocus sativus using Agrobacterium rhizogenes ARqua1 strain with a high transformation efficiency. The protocol can be further utilized for gene transfer or gene editing in Crocus for its genetic improvement.
Article
Biochemistry & Molecular Biology
Penghui Huang, Mingyang Lu, Xiangbei Li, Huiyu Sun, Zhiyuan Cheng, Yuchen Miao, Yongfu Fu, Xiaomei Zhang
Summary: The stable genetic transformation of soybean is time-consuming and inefficient. To address this issue, researchers developed a new efficient method for regeneration and transformation of hairy roots in soybean, achieving high efficiency within a shorter period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Lucas Ripa, G. N. Stevens, E. E. Lewis
Summary: Plants can act as mediators to connect belowground and aboveground heterotrophic communities. This study aimed to determine how the activity of one herbivore affects the acceptability of a plant to another herbivore. The results showed that plants can mediate interactions between belowground and aboveground herbivores.
Article
Plant Sciences
Okhee Choi, Seunghoe Kim, Byeongsam Kang, Yeyeong Lee, Juyoung Bae, Jinwoo Kim
Summary: This study analyzed the genetic diversity of Korean B. glumae isolates, revealing that the isolates were divided into 11 clusters and three divisions, with most of the pigment-producing isolates grouped in divisions II and III.
Article
Microbiology
Okhee Choi, Byeongsam Kang, Yongsang Lee, Yeyeong Lee, Jinwoo Kim
Summary: Carotenoids are widely used in various industries, with this study focusing on characterizing carotenoid biosynthetic genes in the plant-pathogenic bacterium Pantoea ananatis. Through genetic engineering, different carotenoids were produced to confer tolerance to UV radiation and toxoflavin. The molecular mechanism behind carotenoid production in P. ananatis was elucidated, showing a complex regulatory network involving key genes and signaling pathways.
Article
Microbiology
Dimas Hand Vidya Paradhipta, Young Ho Joo, Hyuk Jun Lee, Seong Shin Lee, Hyeon Tak Noh, Jeong Seok Choi, Jinwoo Kim, Hyeong Gyu Min, Sam Churl Kim
Summary: The study found that inoculants can improve the quality of corn silage and extend its resistance against mold contamination at feed-out phase. Using a mixed inoculant shows better effects.
Article
Biotechnology & Applied Microbiology
Okhee Choi, Jaeyeong Cho, Byeongsam Kang, Yeyeong Lee, Jinwoo Kim
Summary: This study identified the essential role of two siderophores, aerobactin and desferrioxamine E, in the antibacterial activity of P. ananatis PA13. The biosynthetic gene clusters of these siderophores were found to be negatively regulated by the Fur protein. Siderophore production was not required for P. ananatis virulence in plants, but it appears to be involved in the microbial ecology surrounding the plant environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Changhoon Lee, Ho Jin Youn, Sang-Hoon Lee, Jinwoo Kim, Daeyoung Son, Joon-Yung Cha
Summary: This study investigated the positive role of plant Aha in heat stress tolerance through chaperone properties and/or activation of Hsp90 to protect substrate proteins from thermal injury.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Maureen C. Onyeziri, Ramya Natarajan, Gail G. Hardy, Jing Xu, Ian P. Reynolds, Jinwoo Kim, Peter M. Merritt, Thomas Danhorn, Michael E. Hibbing, Alexandra J. Weisberg, Jeff H. Chang, Clay Fuqua
Summary: Agrobacterium tumefaciens, a type of bacteria that infects plants and associates with various surfaces, produces a polysaccharide called UPP in two distinct forms, each requiring a specific polymerase. These findings have important implications for understanding polysaccharide biosynthesis mechanisms.
MOLECULAR MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Zhongqing Ren, Qin Liao, Xheni Karaboja, Ian S. Barton, Eli G. Schantz, Adrian Mejia-Santana, Clay Fuqua, Xindan Wang
Summary: Bacterial species with multipartite genomes, such as Agrobacterium tumefaciens, have their circular and linear chromosomes interacting with each other, while plasmids do not show such interactions. There are also two types of interactions observed: clustering of replication origins and linear alignment of chromosome arms. Centromeric proteins are required for these interactions. The study provides a high-resolution view of the organization of multipartite genomes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Zhongqing Ren, Qin Liao, Ian S. Barton, Emma E. Wiesler, Clay Fuqua, Xindan Wang
Summary: This study provides evidence that centromeric clustering, mediated by centromeric proteins, plays a crucial role in maintaining the stability of multipartite genomes. Disruption of centromere clustering results in the loss of replicons.
Article
Microbiology
Minhee Kang, Jae Yun Lim, Jinwoo Kim, Ingyu Hwang, Eunhye Goo
Summary: Bacteria adapt to harsh environments by changing their genetic and physiological traits. A study on Burkholderia glumae strains found that genomic diversity is linked to the ability to adapt to different environments. The study also revealed a correlation between genomic structures and spontaneous mutations in a master regulator gene, as well as nutritional conditions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Okhee Choi, Yeyeong Lee, Byeongsam Kang, Jinwoo Kim
Summary: Toxoflavin contamination in broken rice produced by domestic rice processing complexes in South Korea was investigated in 2011. Out of the 68 complexes investigated, 12 were confirmed to have toxoflavin contamination in three provinces: Gangwon, Gyeonggi, and Gyeongsang. Burkholderia glumae was identified as the toxoflavin-producing bacteria in the rice samples collected from these complexes. The toxoflavin-producing ability varied among the 25 bacterial isolates, but all of them inhibited rice seed germination and induced seed rot. This study provides important baseline data for managing toxoflavin contamination in rice storage and processing.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Jennifer L. L. Greenwich, Melene A. A. Alakavuklar, Clay Fuqua
Summary: A broad host range (BHR)-inducible T7 RNA polymerase system has been developed, which allows for induction with IPTG and is applicable in various bacteria. This system enables high protein yields and purification from different Gram-negative bacteria, including the native host.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Review
Chemistry, Multidisciplinary
Clay Fuqua
Summary: The field of bacterial chemical communication has experienced rapid growth since the late 20th century. The contributions of Bonnie Bassler and her colleagues have been immense and multifaceted. This review discusses the author's own involvement in the research field, their introduction to Dr. Bassler and her early findings, the establishment of the Bassler lab, and the influential experimentation and public outreach that has advanced the field of bacterial chemical communication.
ISRAEL JOURNAL OF CHEMISTRY
(2023)
Review
Microbiology
Pamela J. B. Brown, Jeff H. Chang, Clay Fuqua
Summary: Agrobacterium tumefaciens has been studied since the 17th century, when scientists started investigating the mechanisms behind its pathogenicity. Research over the years has revealed the genetic transfer mechanism by which A. tumefaciens causes crown gall and its significant applications in plant genetic manipulation.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Multidisciplinary Sciences
Okhee Choi, Yeyeong Lee, Byeongsam Kang, Su Kyung Cho, Yongsung Kang, Dong-Wan Kang, Seul-Bi Lee, Sung-Mun Bae, Jinwoo Kim
Summary: This study isolated lactic acid bacteria (LAB) from the gut of a pest called Riptortus pedestris and found that these LAB have the ability to survive under gastrointestinal conditions and have beneficial effects on the insect host. Feeding the LAB increased the survival rate of the insects, but did not affect their weight or length. These findings provide fundamental information about the symbiotic relationship between insects and LAB, as well as a novel concept for pest control.