Article
Chemistry, Analytical
Wenjing Liu, Yuanyu Huang, Zhengping Li, Lele Li, Yuliang Zhao, Mengyuan Li
Summary: This study presents an exosomal engineering strategy that enables controlled regulation of exosome-cell interactions and dynamic monitoring through activatable sensing probes. The engineered exosomes exhibit enhanced cancer cell targeting ability compared to conventional modifications. This approach could contribute to the development of exosome-based biomedical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Heikki Kyykallio, Alessandra V. S. Faria, Rosabella Hartmann, Janne Capra, Kirsi Rilla, Pia R-M Siljander
Summary: This research developed two methods using nanofibrillar cellulose as a 3D cell culture matrix to isolate extracellular vesicles from cancer spheroids. Both methods were easy to set up, quick to perform, and provided high yields of extracellular vesicles.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Review
Oncology
Guillermo Bordanaba-Florit, Iratxe Madarieta, Beatriz Olalde, Juan M. Falcon-Perez, Felix Royo
Summary: 3D cell cultures have improved cancer research by preserving cancer physiological characteristics and facilitating the study of interactions. Extracellular vesicles play key roles in oncology, serving as interactors with the extracellular matrix, cell-to-cell messengers, carriers of biomarkers, and treatment-delivery agents. They are utilized to evaluate tumor progression and potential therapeutic applications, showing promise in drug delivery and antitumoral activity.
Review
Pharmacology & Pharmacy
Mar Casajuana Ester, Richard M. Day
Summary: In recent years, extracellular vesicles (EVs) have emerged as promising biomarkers, cell-free therapeutic agents, and drug delivery carriers. However, the production of EVs remains a challenge. The use of three-dimensional (3D) culture methods, such as scaffolds and bioreactors, has been successful in increasing EV production and enhancing their therapeutic effects. 3D culture methods also provide opportunities to study the role of EVs in disease pathogenesis and improve drug delivery.
Article
Multidisciplinary Sciences
Jessica L. Ruiz, Joshua D. Hutcheson, Luis Cardoso, Amirala Bakhshian Nik, Alexandra Condado de Abreu, Tan Pham, Fabrizio Buffolo, Sara Busatto, Stefania Federici, Andrea Ridolfi, Masanori Aikawa, Sergio Bertazzo, Paolo Bergese, Sheldon Weinbaum, Elena Aikawa
Summary: The study demonstrates that bisphosphonates can alter EV-driven calcification process, affecting microcalcification morphology and risk of plaque rupture. The 3D hydrogel is a viable platform for studying EV-mediated mineral nucleation and evaluating potential therapies for cardiovascular calcification.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Cristina Mas-Bargues, Consuelo Borras
Summary: The progress in understanding the biological properties of extracellular vesicles (EVs) derived from stem cells has been hindered by many misconceptions and methodological issues. Standardization of methodology is necessary in order to compare, validate, and improve the reliability and credibility of the various research findings for the development of EV-based therapeutics.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Engineering, Biomedical
Fabio Maiullari, Maila Chirivi, Marco Costantini, Anna Maria Ferretti, Sandro Recchia, Silvia Maiullari, Marika Milan, Dario Presutti, Valentina Pace, Marcello Raspa, Ferdinando Scavizzi, Massimo Massetti, Lella Petrella, Mara Fanelli, Marta Rizzi, Orazio Fortunato, Fabiola Moretti, Eugenio Caradonna, Claudia Bearzi, Roberto Rizzi
Summary: Extracellular vesicles have shown to be effective in mediating intercellular communication, and recent studies have demonstrated the use of EVs in combination with 3D bioprinting to promote angiogenesis in regenerative medicine.
Article
Chemistry, Multidisciplinary
Ayhan Yurtsever, Takeshi Yoshida, Arash Badami Behjat, Yoshihiro Araki, Rikinari Hanayama, Takeshi Fukuma
Summary: Exosomes have emerged as important mediators of cell-to-cell communication and potential biomarkers for cancer and other diseases. Detailed characterization of their structural, molecular, and biomechanical properties is crucial for identifying disease-related changes. Analysis using 3D-AFM revealed distinct structural and nanomechanical properties of exosomes, with differences in mechanical characteristics observed between metastatic and nonmetastatic tumor cell-derived exosomes.
Article
Multidisciplinary Sciences
Dong Gil You, Gyeong Taek Lim, Seunglee Kwon, Wooram Um, Byeong Hoon Oh, Seok Ho Song, Jungmi Lee, Dong-Gyu Jo, Yong Woo Cho, Jae Hyung Park
Summary: Metabolically engineered exosomes have been shown to effectively reprogram macrophages' immune phenotype, demonstrating potential therapeutic efficacy in treating rheumatoid arthritis.
Review
Biotechnology & Applied Microbiology
Sara Abdollahi
Summary: Initially considered as junk carriers, extracellular vesicles (EVs) have been found to play a crucial role in cell communication by transporting RNA, DNA, and other bioactive components. Recent studies are utilizing 3D cell culture models to analyze EVs in physiological and pathological contexts, although standard and reproducible methods for EV analysis in these models are lacking.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Arnab Banerjee, Miguel Lino, Carlos Jesus, Quelia Ribeiro, Antero Abrunhosa, Lino Ferreira
Summary: Extracellular vesicles (EVs) act as communication vehicles for exchanging bioactive molecules between cells. Understanding their role in cellular communication is crucial for evaluating their biological, diagnostic, and therapeutic potential. Controlled release of EVs for regenerative medicine applications has been explored, and evaluating their release profile is important for correlating with biological activity. This article provides an overview of EV imaging platforms, discusses different EV labeling processes, and highlights the relevance of imaging platforms in studying the tropism, biological role, and release profile of EVs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Lilibeth Ortega-Pineda, Alec Sunyecz, Ana I. Salazar-Puerta, Maria Angelica Rincon-Benavides, Diego Alzate-Correa, Amrita Lakshmi Anaparthi, Elizabeth Guilfoyle, Louisa Mezache, Heather L. Struckman, Silvia Duarte-Sanmiguel, Binbin Deng, David W. McComb, Daniel J. Dodd, William R. Lawrence, Jordan Moore, Jingjing Zhang, Eduardo Reategui, Rengasayee Veeraraghavan, M. Tyler Nelson, Daniel Gallego-Perez, Natalia Higuita-Castro
Summary: Gene/oligonucleotide therapies have shown potential as a treatment strategy for neurological conditions, but current delivery methods have limitations. Extracellular vesicles (EVs) have emerged as a promising delivery vehicle, overcoming many of these limitations, including low permeability of the blood-brain barrier. This study provides a detailed understanding of the manufacturing process of neurogenic EVs and demonstrates their ability to enhance neuron-specific tropism and modulate electrophysiological activity. The study also suggests that surface decoration with glutamate receptors can facilitate enhanced delivery of EVs to the brain. Overall, these findings suggest that designer EVs loaded with specific factors can be a promising nanotechnology platform to drive pro-neuronal responses.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemical Research Methods
Maria Y. Pakharukova, Ekaterina Savina, Dmitry V. Ponomarev, Natalya V. Gubanova, Oxana Zaparina, Elvira G. Zakirova, Guofeng Cheng, Olga V. Tikhonova, Viatcheslav A. Mordvinov
Summary: This study describes for the first time the release of extracellular vesicles (EVs) by the food-borne trematode Opisthorchis felineus, and characterizes their microscopic and proteomic properties, as well as their internalization pathways by human cholangiocytes. The differential abundance of proteins between whole adult worms and EVs was assessed, revealing enrichment of canonical EV markers and parasite specific proteins. These findings provide insights into the important role of EV cargo components in the pathogenesis of fluke infection and bile duct neoplasia.
JOURNAL OF PROTEOMICS
(2023)
Correction
Gastroenterology & Hepatology
J. Shapiro, H. B. El-Serag, J. Chan
Summary: This article reports the inflammation in mice with steatohepatitis and acknowledges the inclusion of duplicate images in the supplementary figures. The corrected figures have been presented.
Article
Cell Biology
Alexandre Brodeur, Francis Migneault, Maude Lanoie, Deborah Beillevaire, Julie Turgeon, Annie Karakeussian-Rimbaud, Nicolas Thibodeau, Eric Boilard, Melanie Dieude, Marie-Josee Hebert
Summary: This study elucidates the mechanisms by which endothelial cells internalize ApoExos and reveals the functional transfer of specific mRNA from ApoExos to endothelial cells.
CELL DEATH & DISEASE
(2023)