期刊
BIOTECHNOLOGY LETTERS
卷 38, 期 8, 页码 1359-1365出版社
SPRINGER
DOI: 10.1007/s10529-016-2115-2
关键词
Acetone; Escherichia coli; Non-oxidative glycolysis; Phosphoketolase
资金
- National Key Basic Research Program of China (973 Program) [2012CB725203, 2015CB755704]
- Key Project in the Tianjin Science & Technology Pillar Program [14ZCZDSY 00060]
To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical acetone yield from 1 to 1.5 mol acetone/mol glucose. By inserting the fxpk gene encoding phosphoketolase from Bifidobacterium adolescentis into the genome, we constructed a NOG pathway in E.coli. The resulting strain produced 47 mM acetone from glucose under aerobic conditions in shake-flasks. The yield of acetone was improved from 0.38 to 0.47 mol acetone/mol glucose which is a significant over the parent strain. Guided by computational analysis of metabolic networks, we introduced a NOG pathway into E. coli and increased the yield of acetone, which demonstrates the importance of modeling analysis for the novel metabolic engineering strategies.
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