期刊
BIOTECHNOLOGY LETTERS
卷 38, 期 8, 页码 1397-1404出版社
SPRINGER
DOI: 10.1007/s10529-016-2111-6
关键词
Adipose-derived stem cells; Cryopreservation; Freezing solution; Hydrocolloids; Organic osmolytes; Serum-free
资金
- Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India [BT/01/COE/09/1309062010]
- Department of Science and Technology, Ministry of Science and Technology, Government of India [SR/FTP/ETA-0106/2011]
To develop a cost-effective, non-toxic and xeno-free freezing solution for the preservation of adipose tissue-derived stem cells (hADSC) with a long shelf-life. The potential of various hydrocolloids and organic osmolytes as cryoprotectants and individual components of phosphate buffered saline (PBS) as carrier media were evaluated to formulate a freezing solution for the cryopreservation of hADSCs. Among the hydrocolloids, the highest viability, 55 %, was achieved with post-thawed (after 48 h storage at -80 A degrees C) hADSCs cryopreserved in 10 % (v/v) polyvinylpyrrolidone (PVP) using PBS as carrier media. 0.9 % NaCl was a superior carrier medium resulting an enhanced cell viability (70 %) when used in 10 % PVP than other components of PBS. A higher cell viability (81 %) was achieved when 10 % PVP/0.9 % NaCl was supplemented with 60 mM ectoin. The cryopreserved cells retained normal cytoskeletal distribution pattern and adipogenic and osteogenic differentiation ability during 14 and 21 days of incubation. A serum-free and non-toxic 10 % PVP/0.9 % NaCl/60 mM ectoin freezing solution was developed for cryopreservation of hADSC for application in tissue engineering and regenerative medicine.
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