期刊
ONCOTARGETS AND THERAPY
卷 12, 期 -, 页码 10099-10105出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S224053
关键词
EMT; PD-L1; migration and invasion; gastric carcinoma
资金
- school-level subject of the First Affiliated Hospital of Shihezi University School of Medicine [YL2016-R004]
- 8th Shihezi City Science and Technology Plan Project [2017H220]
Purpose: The aim of this study was to investigate the influence of epithelial-mesenchymal transition (EMT) occurring in gastric carcinoma cells and the involvement of programmed death ligand 1 (PD-L1) expression in tumor cells that undergo EMT. The mechanisms underlying PD-L1 expression during EMT in gastric carcinoma cells were also explored. Methods: The capacities of migration and invasion were tested by cell scratch-wound assay and transwell chamber assay. PD-L1 expression by SGC7901 cell line and related mechanism were measured by Western blot and QRT-PCR. Results: Treating with TGF-beta 1 promotes the motility of SGC7901 and PD-L1 expression in vitro, while activating the NF-kappa B signal pathway. Conclusion: EMT increases the capacities of migration and invasion in gastric cancer cells, which resulted in up-regulation of PD-L1 expression via a mechanism that is dependent on NF-kappa B activation.
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