Article
Chemistry, Multidisciplinary
Masayuki Karasawa, Kai Yonemura, Joshua Kyle Stanfield, Kazuto Suzuki, Osami Shoji
Summary: The study shows that the cellular uptake of external additives by Escherichia coli whole-cell biocatalysts can be significantly improved through the use of an OmpF loop deletion mutant and decoy molecules, leading to increased benzene biotransformation yield and enantioselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Qiu-Hua Zhang, Liu Yang, Yi-Bin Tang, Liu-Nv Huang, Wen-Fang Luo
Summary: The study utilized immobilized whole-cells of Pichia pastoris in calcium alginate gels as an efficient biocatalyst for the catalytic kinetic resolution of d,l-pantolactone. The biocatalyst showed good catalytic stability, enabling stereospecific hydrolysis of d-pantolactone for up to 56 repeated batch reactions without significant loss in catalytic activity and enantioselectivity.
Article
Biotechnology & Applied Microbiology
Baodong Hu, Xinrui Zhao, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du
Summary: This study reports the development of a bacterial P450 sca-2(mut) whole-cell biocatalyst with remarkable 3'-hydroxylation activity for efficient hydroxylation of flavonoids. Flavodoxin Fld and flavodoxin reductase Fpr from Escherichia coli were used to enhance the whole-cell activity of sca-2(mut). Through enzymatic engineering, the double mutant of sca-2(mut) (R88A/S96A) exhibited improved hydroxylation performance for flavonoids. The optimized whole-cell biocatalytic conditions further enhanced the whole-cell activity of sca-2(mut) (R88A/S96A). Eriodictyol, dihydroquercetin, luteolin, and 7,3',4'-trihydroxyisoflavone were successfully produced as functionalized derivatives of flavonoids using naringenin, dihydrokaempferol, apigenin, and daidzein as substrates, with conversion yields of 77%, 66%, 32%, and 75% respectively. The strategy employed in this study offers an effective method for the hydroxylation of other high value-added compounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Yaping Mao, Zhuo Chen, Yuhong Ren, Yuwei Sun, Yong Wang
Summary: In this study, a whole-cell biocatalyst was designed to glycosylate steviol to Rub in yeast. By optimizing reaction conditions and gene mutations, a high efficiency method for producing Rub was developed, laying the foundation for scalable production of this value-added diterpene sweetener in the future.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Engineering, Environmental
Jing Ji, Tianpeng Gao, El-Sayed Salama, Marwa M. El-Dalatony, Liang Peng, Yuxing Gong, Pu Liu, Xiangkai Li
Summary: This study developed a novel whole-cell biocatalyst to accelerate the degradation of antibiotics in pharmaceutical wastewater. By displaying β-lactamase on the cell surface, the biocatalyst showed high enzyme activity and stable performance, significantly improving antibiotic removal and overall reactor performance.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Raphael Loll-Krippleber, Victoria A. Sajtovich, Michael W. Ferguson, Brandon Ho, Andrew R. Burns, Brandon J. Payliss, Joseph Bellissimo, Sydney Peters, Peter J. Roy, Haley D. M. Wyatt, Grant W. Brown
Summary: In this study, a whole-cell catalyst expressing the PET degrading enzyme MHETase was developed using Saccharomyces cerevisiae as a platform. The expressed MHETase on the yeast cell surface showed comparable activity to recombinant MHETase from Escherichia coli. Furthermore, the surface displayed MHETase exhibited activity across various conditions and could potentially serve as an alternative to protein purification-based approaches for plastic degradation.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Zhenzhen Cui, Meiyu Zheng, Mengnan Ding, Wei Dai, Zhiwen Wang, Tao Chen
Summary: In this study, a series of engineered strains of Escherichia coli were constructed to produce acetoin from lactate. After optimization, high titers and yields of acetoin were achieved in shake flasks and bioreactors. This is the first report on the production of acetoin from renewable lactate with both high titer and yield, demonstrating the economic and efficient production of acetoin from lactate.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sara Arana-Pena, Diego Carballares, Roberto Morellon-Sterlling, Angel Berenguer-Murcia, Andres R. Alcantara, Rafael C. Rodrigues, Roberto Fernandez-Lafuente
Summary: Enzyme co-immobilization offers kinetic advantages but also drawbacks, such as the need to discard the entire biocatalyst if one enzyme becomes inactivated. The co-immobilization of cofactors and enzymes is of particular interest for the construction of artificial cells and complex one-pot transformations.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Agriculture, Multidisciplinary
Junbin Ji, Caiting Zeng, Panpan Wu, Yuying Wang, Xueting Chen, Xin Yan
Summary: This study successfully improved the synthesis efficiency of 2,3,5-Trimethylhydroquinone (2,3,5-TMHQ) through enzyme modification and adaptive laboratory evolution, providing better genetic resources for its production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Meiqi Chen, Xin Zeng, Qingjuan Zhu, Denggang Wang, Shuangyan Han, Shuli Liang, Ying Lin
Summary: The study found that sucrose synthase mbSUS from Vigna radiata can effectively cooperate with glycosyltransferase UGT76G1 from Stevia rebaudiana Bertoni, leading to an increased efficiency in synthesizing Rebaudioside A.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Agriculture, Multidisciplinary
Haixia Xu, Xiaofeng Li, Xuan Xin, Lan Mo, Yucong Zou, Guanglei Zhao, Yigang Yu, Kebing Chen
Summary: In this study, a green solvent system was developed to synthesize arbutin esters with improved liposolubilities, among which arbutin undecenoate (AU) showed the strongest tyrosinase-inhibiting activity compared to arbutin. AU was found to be a competitive inhibitor with reversible inhibition, with its mechanism of tyrosinase inhibition involving making the secondary structure of the enzyme looser and less stable. Additionally, AU significantly reduced melanin content in B16 mouse melanoma cells.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Environmental Sciences
Eugene Huang, Jhen-Sheng Yan, Ronnie G. Gicana, Yin-Ru Chiang, Fang- Yeh, Chieh-Chen Huang, Po-Hsiang Wang
Summary: The disposal of soybean pulp (okara) is a global concern. It is possible to use okara as an economical alternative for bioproduction of alpha-ketoisocaproate (KIC), which is a metabolite with potential in animal husbandry. A cost-efficient platform has been developed to valorize okara into KIC, using a proteolytic Bacillus subtilis strain as a biocatalyst.
Article
Biochemistry & Molecular Biology
Min-Yu Wang, Shun-Ju Cai, Jia-Chun Lin, Xiao-Jun Ji, Zhi-Gang Zhang
Summary: A new bacterial strain, Bacillus cereus TQ-2, capable of catalyzing the conversion of ketones to enantiopure alcohols with high activity and excellent enantioselectivity was discovered. The strain showed optimal performance at a temperature of 30 degrees C and a pH range of 5.0-9.0. Addition of glycerol, Ca2+ or K+ ions enhanced the catalytic activity. The versatile whole-cell biocatalyst demonstrated efficient reduction of diverse ketones and aldehydes to form corresponding alcohols.
Review
Green & Sustainable Science & Technology
Wan Yuen Tan, Subash C. B. Gopinath, Periasamy Anbu, Ahmad Radi Wan Yaakub, Sreeramanan Subramaniam, Yeng Chen, Sreenivasan Sasidharan
Summary: With the development of bionanotechnology, the field of nanobiocatalysts has grown rapidly and transformed various nanomaterials into novel nanocarriers for enzyme immobilization. Nanotubes, nanofibers, nanopores, nanoparticles, and nanocomposites have been successfully used as nanocarriers. The combination of enzymes and nanocarriers through different immobilization techniques has attracted attention due to their exceptional catalytic performance, stability, and reusability. The review discusses enzyme immobilization on nanocarriers and highlights the techniques, properties, preparations, and applications of nanoimmobilized enzymes.
Article
Chemistry, Organic
Zeinab Salehi, Ali Rasouli, Hamid Doosthosseini
Summary: In this study, free R. eutropha was adapted to degrade PNP, demonstrating nongrowth biodegradation and noncompetitive substrate-inhibitory reaction. Through analysis using an enzymes-substrate complex model, a theoretical mechanism involving four reactions was confirmed, including enzyme production, enzyme-substrate complex equilibrium, inhibition of enzyme-substrate complex equilibrium, and substrate decomposition.
POLYCYCLIC AROMATIC COMPOUNDS
(2021)