Article
Virology
Mengxin Zhou, Yutong Han, Mengxia Li, Gang Ye, Guiqing Peng
Summary: This study identified two compounds that target the 3C-like protease (3CL(pro)) of porcine epidemic diarrhoea virus (PEDV) and exhibit antiviral capacity against feline infectious peritonitis virus (FIPV). These compounds may aid in the development of anti-coronavirus drugs in the future.
Article
Veterinary Sciences
Tomoyoshi Doki, Ken Takahashi, Nobuhisa Hasegawa, Tomomi Takano
Summary: The combination of nucleoside analog GS-441524 and triazole antifungal drug itraconazole has a synergistic antiviral effect against the feline infectious peritonitis virus (FIPV), which causes a fatal disease in cats.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
N. Decaro, V Mari, G. Lanave, E. Lorusso, M. S. Lucente, C. Desario, M. L. Colaianni, G. Elia, F. Ferringo, F. Alfano, C. Buonavoglia
Summary: The study investigated the frequency of mutations associated with virulence in FIPV and FECV strains circulating in Italian cats. Mutations M1058L and S1060A were found to be present in FIPV strains, highlighting the importance of genetic signatures in the virulence shift of FECV/FIPV.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Jimmy Lu, Sizhu Amelia Chen, Muhammad Bashir Khan, Raelynn Brassard, Elena Arutyunova, Tess Lamer, Wayne Vuong, Conrad Fischer, Howard S. Young, John C. Vederas, M. Joanne Lemieux
Summary: Coronaviruses infect a variety of hosts in the animal kingdom, and GC376 has been shown to treat feline infectious peritonitis. The study found that GC373 and GC376 are drugs with strong inhibitory effects on SARS-CoV-2, and they also have strong inhibitory effects on FIPV M-pro, which is important for structure-guided drug design.
FRONTIERS IN CHEMISTRY
(2022)
Article
Microbiology
Shih-Jung Yen, Hui-Wen Chen
Summary: In northern Taiwan, samples from cats suspected of feline infectious peritonitis showed that younger cats are at a significantly higher risk than older cats. There was no significant association between positivity rates and sex or breed. Positive cases had a lower A/G ratio and showed a high diversity in type I sequences, while type II sequences were more similar to coronaviruses from other species.
Review
Virology
Yong-Yu Gao, Qian Wang, Xiang-Yu Liang, Shuang Zhang, Di Bao, Han Zhao, Shao-Bai Li, Kai Wang, Gui-Xue Hu, Feng-Shan Gao
Summary: This article reviews recent progress in FCoV research, including the etiological characteristics, epidemiology, clinical symptoms and pathological changes, pathogenesis, and current diagnosis, prevention, and treatment methods. It is hoped that this review will provide a reference for further research on FCoV and other coronaviruses.
Article
Veterinary Sciences
Jinbao Lv, Yang Bai, Yingyun Wang, Liu Yang, Yipeng Jin, Jun Dong
Summary: The combination of GS-441524 and GC376 is a safe and effective treatment for FIP, with a shortened treatment period of 4 weeks and an excellent cure rate.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Jong-Chul Choi, Sung-Won Jung, In-Yeong Choi, Yeong-Lim Kang, Dong-Hun Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee, Changin Oh
Summary: Rottlerin has inhibitory effects on FCoV replication and affects different stages of FCoV activity. The use of liposome encapsulation technique improves its efficacy. Therefore, further investigation of rottlerin-liposome as a potential treatment for FCoV is suggested.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Magdalena Dunowska, Sayani Ghosh
Summary: The study found that doxycycline can inhibit the replication of feline infectious peritonitis virus (FIPV) in vitro, with the inhibitory effect being dose-dependent. This supports future clinical trials of using doxycycline to treat FIP-affected cats.
Article
Chemistry, Medicinal
Joo-Youn Lee, Chih-Jung Kuo, Jin Soo Shin, Eunhye Jung, Po-Huang Liang, Young-Sik Jung
Summary: This study conducted virtual screening and in vitro assays to find non-covalent inhibitors targeting 3CLpro in SARS-CoV-2, identifying a promising compound 7 with potent antiviral effects.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Veterinary Sciences
Michele Camero, Gianvito Lanave, Cristiana Catella, Maria Stella Lucente, Alessio Sposato, Viviana Mari, Maria Tempesta, Vito Martella, Alessio Buonavoglia
Summary: ERDRP has been shown to be highly effective against a type II FIPV in vitro, inhibiting viral replication in a dose-dependent manner. Further experiments are needed to determine if ERDRP is suitable for the treatment of FIPV in vivo.
BMC VETERINARY RESEARCH
(2022)
Article
Virology
Sarah Cook, Diego Castillo, Sonyia Williams, Christine Haake, Brian Murphy
Summary: Feline infectious peritonitis (FIP) is caused by genetic variants of feline coronavirus (FCoV) and feline infectious peritonitis virus (FIPV), currently classified into two serotypes, I and II. While serotype II FIPV has been more extensively studied, recent advances include the development of a culture-adapted feline cell line for improved understanding of serotype I FIPV biology and research on coronavirus receptors in feline cells.
Review
Infectious Diseases
Saverio Paltrinieri, Alessia Giordano, Angelica Stranieri, Stefania Lauzi
Summary: Feline infectious peritonitis (FIP) and COVID-19, caused by coronaviruses, share some similar pathogenic and immunopathogenic events. Preventive measures recommended for preventing SARS-CoV-2 spread have also shown success in eradicating FIP in feline households. Additionally, the therapeutic compound GS-441524, effective against FIP, is the active form of Remdesivir used as a therapeutic option for COVID-19.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Virology
Gang Wang, Guangli Hu, Rui Liang, Jiale Shi, Xiuxiu Qiu, Yiling Yang, Zhe Jiao, Yixi Chen, Zhou Shen, Mengxia Li, Yuejun Shi, Junfu Mao, Guiqing Peng
Summary: This study successfully constructed an effective infection model of feline infectious peritonitis and achieved reverse genetics of different virulent and attenuated feline coronaviruses. High titers of neutralizing antibodies were found to potentially prolong the survival time of cats.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Yoshikazu Tanaka, Eri Tanabe, Yuki Nonaka, Mitsuki Uemura, Tsuyoshi Tajima, Kazuhiko Ochiai
Summary: Feline coronaviruses (FCoVs) are a global infection in cats, causing severe systemic diseases. The current drugs for treating FIP are ineffective, highlighting the need to investigate host factors and replication functions for FCoV in order to develop effective drugs. Ionophore antibiotics, by altering the pH conditions in the endosomes, inhibit FCoV proliferation in vitro, showing potential as broad-spectrum anti-FCoV agents.
Article
Chemistry, Medicinal
Chamandi S. Dampalla, Yunjeong Kim, Naemi Bickmeier, Athri D. Rathnayake, Harry Nhat Nguyen, Jian Zheng, Maithri M. Kashipathy, Matthew A. Baird, Kevin P. Battaile, Scott Lovell, Stanley Perlman, Kyeong-Ok Chang, William C. Groutas
Summary: Novel inhibitors have been synthesized that effectively inhibit coronavirus protease, showing potential for treating COVID-19 and providing a means to access new chemical space and optimize pharmacological activity.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Chamandi S. Dampalla, Jian Zheng, Krishani Dinali Perera, Lok-Yin Roy Wong, David K. Meyerholz, Harry Nhat Nguyen, Maithri M. Kashipathy, Kevin P. Battaile, Scott Lovell, Yunjeong Kim, Stanley Perlman, William C. Groutas, Kyeong-Ok Chang
Summary: The study presents a series of deuterated 3CLpro inhibitor variants, particularly GC376, showing potent antiviral effects against SARS-CoV-2 in cell-based and enzyme assays. Treatment with a deuterated derivative of GC376 increased survival rates in lethally infected mice and reduced lung virus titers and histopathological changes. Structural investigation revealed promising potential of deuterated GC376 variants as effective antiviral agents against SARS-CoV-2 through their binding interactions with 3CLpro.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Erin E. Schirtzinger, Yunjeong Kim, A. Sally Davis
Summary: The SARS-CoV-2 pandemic has renewed interest in human coronaviruses that cause the common cold. This study investigated the growth parameters of HCoV-OC43 in different cell lines, finding that HCoV-OC43 grown in MRC-5 cells reached a peak titer at two days post infection, while in HRT-18 cells, it required six days.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Chemistry, Medicinal
Chamandi S. Dampalla, Athri D. Rathnayake, Anushka C. Galasiti Kankanamalage, Yunjeong Kim, Krishani Dinali Perera, Harry Nhat Nguyen, Matthew J. Miller, Trent K. Madden, Hunter R. Picard, Hayden A. Thurman, Maithri M. Kashipathy, Lijun Liu, Kevin P. Battaile, Scott Lovell, Kyeong-Ok Chang, William C. Groutas
Summary: The 3C-like protease (3CLpro) of SARS-CoV-2 has been validated as an effective target for developing therapeutics. Highly potent inhibitors have been successfully designed based on structure-guided design, and their mechanism of action has been established using high-resolution cocrystal structures.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Yunjeong Kim, Natasha N. Gaudreault, David A. Meekins, Krishani D. Perera, Dashzeveg Bold, Jessie D. Trujillo, Igor Morozov, Chester D. McDowell, Kyeong-Ok Chang, Juergen A. Richt
Summary: This study reveals the host range of SARS-CoV-2 and the effects of recently emergent S protein substitutions on viral entry, virus replication, and antibody-mediated viral neutralization, with particular emphasis on the significant impact of substitutions in the S protein of the Omicron variant on antibody neutralization.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Krishani Dinali Perera, David Johnson, Scott Lovell, William C. Groutas, Kyeong-Ok Chang, Yunjeong Kim
Summary: This study investigated the characteristics of lagoviruses such as Rabbit hemorrhagic disease and European brown hare syndrome, and their impact on animals. The key role of the 3C-like protease (3CLpro) in virus replication was also explored. The study identified potential antiviral drugs that target this protease, providing hope for the development of treatments for lagoviruses.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Kurt J. Vandegrift, Michele Yon, Meera Surendran Nair, Abhinay Gontu, Santhamani Ramasamy, Saranya Amirthalingam, Sabarinath Neerukonda, Ruth H. Nissly, Shubhada K. Chothe, Padmaja Jakka, Lindsey LaBella, Nicole Levine, Sophie Rodriguez, Chen Chen, Veda Sheersh Boorla, Tod Stuber, Jason R. Boulanger, Nathan Kotschwar, Sarah Grimke Aucoin, Richard Simon, Katrina L. Toal, Randall J. Olsen, James J. Davis, Dashzeveg Bold, Natasha N. Gaudreault, Krishani Dinali Perera, Yunjeong Kim, Kyeong-Ok Chang, Costas D. Maranas, Juergen A. Richt, James M. Musser, Peter J. Hudson, Vivek Kapur, Suresh V. Kuchipudi
Summary: There is growing evidence of SARS-CoV-2 spillover from humans to various animal species, including deer. This study investigated the active and asymptomatic infection of the Omicron variant in free-ranging deer in New York City. The findings revealed that a small number of deer had been exposed to SARS-CoV-2, with two deer showing evidence of reinfection or early seroconversion. This suggests that variants of concern are still circulating and may reestablish transmission cycles in US deer populations, highlighting the need for a coordinated One Health response.
Article
Chemistry, Medicinal
Chamandi S. Dampalla, Harry Nhat Nguyen, Athri D. Rathnayake, Yunjeong Kim, Krishani Dinali Perera, Trent K. Madden, Hayden A. Thurman, Alexandra J. Machen, Maithri M. Kashipathy, Lijun Liu, Kevin P. Battaile, Scott Lovell, Kyeong-Ok Chang, William C. Groutas
Summary: The emergence of SARS-CoV-2 has led to the urgent need for effective countermeasures, such as vaccines and direct-acting antivirals. This study focuses on the design of highly potent inhibitors for SARS-CoV-2 and other related coronaviruses. The results show promising candidates with low cytotoxicity and high potency for further development as broad-spectrum antivirals.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Ruiqi Huang, Gabrielle Warner Jenkins, Yunjeong Kim, Robyn L. Stanfield, Amrinder Singh, Maria Martinez-Yamout, Gerard J. Kroon, Jonathan L. Torres, Abigail M. Jackson, Abigail Kelley, Namir Shaabani, Baisen Zeng, Michael Bacica, Wen Chen, Christopher Warner, Jasmina Radoicic, Joongho Joh, Krishani Dinali Perera, Huldah Sang, Tae Kim, Jianxiu Yao, Fangzhu Zhao, Devin Sokd, Dennis R. Burton, Jeff Allen, William Harriman, Waithaka Mwangi, Donghoon Chung, John R. Teijaro, Andrew B. Ward, H. Jane Dyson, Peter E. Wright, Ian A. Wilson, Kyeong-Ok Chang, Duncan Mcgregor, Vaughn V. Smider
Summary: Antibodies derived from cows have the potential to be a new class of therapeutics, capable of neutralizing various SARS-CoV-2 variants and providing protection against disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)