Article
Chemistry, Physical
Antonio Valle, Anouar Hailaf, Alvaro Ceballos, Domingo Cantero, Jorge Bolivar
Summary: The combustion of fossil fuels is causing global warming due to greenhouse gas emissions. Hydrogen (H-2) is a clean and sustainable energy source, which can be produced by modifying Escherichia coli through metabolic engineering. Malate plays a crucial role in H-2 synthesis, and redirecting C-flux from malate to H-2 can increase production efficiency.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Review
Chemistry, Analytical
Christopher J. Matthews, Emma S. V. Andrews, Wayne M. Patrick
Summary: This review systematically outlines the progress in enzyme-based amperometric biosensors for monitoring malic acid concentration, highlighting advancements in sensitivity and interference reduction. However, a trade-off between sensitivity and linear range is identified. Future research may focus on identifying or engineering superior alternatives to improve the commercial utility of malic acid biosensors.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biotechnology & Applied Microbiology
Hai-De Wang, Jian-Zhong Xu, Wei-Guo Zhang
Summary: In this study, a high L-arginine (L-Arg) producing E. coli strain was obtained through metabolic engineering. The genes speC and speF were found to play important roles in L-Arg accumulation. Feedback inhibition of the L-Arg pathway in E. coli was successfully overcome by combining gene mutations/deletions. Additionally, the insertion of a gene cluster from Corynebacterium glutamicum enhanced the metabolic flux of the L-Arg pathway. The final strain achieved the highest reported level of L-Arg production by E. coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Janine Hofmann, Mebratu A. Bitew, Miku Kuba, David P. De Souza, Hayley J. Newton, Fiona M. Sansom
Summary: Coxiella burnetii is capable of synthesizing lactate, but the putative lactate synthesizing enzymes CBU1241 and CBU0823 were found to not produce lactate. Loss of CBU0823 affected central carbon metabolism but did not impair bacterial replication inside cells.
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemical Research Methods
Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao, Guang-Rong Zhao
Summary: This study successfully improved the de novo production of nerol and borneol in E. coli by combining metabolic and plant monoterpene synthase engineering, achieving the highest reported titers of nerol and borneol in microbes to date.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Mengyang Lei, Zetian Qiu, Leilei Guan, Zheng Xiang, Guang-Rong Zhao
Summary: In this study, a metabolic engineering approach was employed to produce Z,Z-farnesol (Z,Z-FOH) in Escherichia coli. By testing various enzymes and mutants, the optimal strain was able to achieve the highest reported titer of Z,Z-FOH in microbes. This work not only provides insights into the de novo biosynthesis of Z,Z-FOH in E. coli, but also lays a foundation for the development of synthetic E. coli cell factories for other cis-configuration terpenoids.
Article
Biotechnology & Applied Microbiology
Mengmeng Cai, Zhenqiang Zhao, Xiangfei Li, Yuanyi Xu, Meijuan Xu, Zhiming Rao
Summary: In this study, a wild-type Escherichia coli was converted to a high-producer of L-homoserine using metabolic engineer strategies. By regulating the degradation pathway, optimizing copy numbers, and modifying the transport system, a final engineered strain was able to efficiently produce a high level of L-homoserine.
METABOLIC ENGINEERING
(2022)
Article
Agricultural Engineering
Yu Zhang, Minhua Wei, Guihong Zhao, Wenjie Zhang, Yingzi Li, Beibei Lin, Yanjun Li, Qingyang Xu, Ning Chen, Chenglin Zhang
Summary: In this study, a high-efficiency Escherichia coli strain capable of producing 60.1 g/L L-homoserine was constructed, achieving the highest fermentative production efficiency of L-homoserine to date. By dynamically attenuating the L-homoserine degradation pathway and employing systems metabolic engineering strategies, the production yield of L-homoserine was significantly increased. This engineered strain can be further applied for scale-up production of L-homoserine and its derivatives.
BIORESOURCE TECHNOLOGY
(2021)
Article
Food Science & Technology
Qiling Chen, Nan Hao, Lili Zhao, Xiangke Yang, Yuxin Yuan, Yuzhu Zhao, Fu Wang, Zuobing Qiu, Ling He, Kan Shi, Shuwen Liu
Summary: In this study, the functions of genes encoding malic enzyme (ME) and malolactic enzyme (MLE) were verified through genetic manipulation in L. plantarum. The results showed that MLE was the only enzyme responsible for direct malate metabolism under acid stress in both O. oeni and L. plantarum. Furthermore, O. oeni exhibited significantly higher malate metabolism activity than L. plantarum under acid stress.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Shuai Jiang, Ruirui Wang, Dehu Wang, Chunguang Zhao, Qian Ma, Heyun Wu, Xixian Xie
Summary: This study combined multilevel rational metabolic engineering with biosensor-assisted mutagenesis screening to exploit the L-arginine production potential of Escherichia coli. Multiple metabolic pathways were reprogrammed to redirect the metabolic flux into L-arginine synthesis, and a toggle switch was designed to dynamically control gene expression. A biosensor-assisted high-throughput screening platform was used to further explore L-arginine production potential. Through this approach, the engineered strain produced the highest values of L-arginine reported so far. The study demonstrated the effectiveness of rational metabolic reprogramming and biosensor-assisted mutagenesis screening in unleashing cellular potential for value-added metabolite production.
METABOLIC ENGINEERING
(2023)
Article
Biochemical Research Methods
Xuecong Cen, Yu Liu, Bo Chen, Dehua Liu, Zhen Chen
Summary: A nonnatural pathway was designed and constructed for the de novo production of 1,5-Pentanediol from cheap carbohydrates. Through systematic enzyme screening, pathway balancing, and transporter engineering, a minimally engineered Escherichia coli strain capable of producing 1,5-Pentanediol was successfully developed. This work lays the foundation for a biological route for 1,5-Pentanediol production from renewable bioresources.
ACS SYNTHETIC BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Zhen Wei, Yongxue Xu, Qing Xu, Wei Cao, He Huang, Hao Liu
Summary: Malic acid, a four-carbon dicarboxylic acid, is widely used in various industries and can be produced from renewable sources through microbial fermentation. With advances in genetic engineering, promising strains have been developed for large-scale bio-based production of malic acid. The review highlights recent developments in the fermentative production of malic acid and discusses metabolic engineering strategies to improve production efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Wenwei Li, Zhen Zhou, Dan Wang
Summary: This paper summarizes the advantages and disadvantages of chemical hydrolysis, enzymatic biotransformation, and fermentation for the synthesis of L-cysteine. It provides a detailed introduction to the biosynthesis of L-cysteine, metabolic engineering strategies, and the latest progress in reported L-cysteine fermentation bacteria. Insights are given on the future development of increasing the production of biosynthetic L-cysteine.
FERMENTATION-BASEL
(2023)
Article
Endocrinology & Metabolism
Alexander Chou, Seung Hwan Lee, Fayin Zhu, James M. Clomburg, Ramon Gonzalez
Summary: The study introduces an alternative approach using synthetic pathways for C1 bioconversion, generating multicarbon products directly from formate, formaldehyde and methanol while being orthogonal to the host metabolic network. Variants of the FORCE pathways were evaluated through thermodynamic and stoichiometric analyses, showing promising results both in vitro and in vivo. This synthetic metabolic pathway demonstrates the potential for C1 compound utilization and integration with host metabolism for synthetic methylotrophy.
Review
Biotechnology & Applied Microbiology
Wei Song, Xiulai Chen, Jing Wu, Jianzhong Xu, Weiguo Zhang, Jia Liu, Jian Chen, Liming Liu
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Rui Yao, Pei Zhou, Chengjin Wu, Liming Liu, Jing Wu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Guoxing Zhu, Nannan Yin, Qiuling Luo, Jia Liu, Xiulai Chen, Liming Liu, Jianrong Wu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Nannan Yin, Guoxing Zhu, Qiuling Luo, Jia Liu, Xiulai Chen, Liming Liu
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Jian-Zhong Xu, Hao-Zhe Ruan, Hai-Bo Yu, Li-Ming Liu, Weiguo Zhang
MICROBIAL CELL FACTORIES
(2020)
Article
Chemistry, Physical
Liang Guo, Wenwen Diao, Cong Gao, Guipeng Hu, Qiang Ding, Chao Ye, Xiulai Chen, Jia Liu, Liming Liu
Article
Biotechnology & Applied Microbiology
Chengjin Wu, Guoxing Zhu, Qiang Ding, Pei Zhou, Jie Liu, Xiulai Chen
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Qiang Ding, Danlei Ma, Gao-Qiang Liu, Yang Li, Liang Guo, Cong Gao, Guipeng Hu, Chao Ye, Jia Liu, Liming Liu, Xiulai Chen
NATURE COMMUNICATIONS
(2020)
Article
Biotechnology & Applied Microbiology
Xiulai Chen, Juyang Yi, Wei Song, Jia Liu, Qiuling Luo, Liming Liu
Summary: Chassis engineering was used to enhance enzymatic production of Hyp in Escherichia coli, leading to significant improvements in Hyp titre, conversion rate and productivity. Rewiring the TCA cycle and l-proline degradation pathway increased alpha-ketoglutarate supply, while fine-tuning heterologous haemoglobin expression improved oxygen transfer, resulting in a more efficient method for industrial production of Hyp.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiulai Chen, Juyang Yi, Jia Liu, Qiuling Luo, Liming Liu
Summary: This study presents a green and efficient production process for Hyp by overexpressing DsP4H enzyme and optimizing culture parameters, achieving high yields and conversion rates.
MICROBIAL BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Qiang Ding, Wenwen Diao, Cong Gao, Xiulai Chen, Liming Liu
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Jianshen Hou, Cong Gao, Liang Guo, Jens Nielsen, Qiang Ding, Wenxiu Tang, Guipeng Hu, Xiulai Chen, Liming Liu
METABOLIC ENGINEERING
(2020)
Review
Biochemical Research Methods
Liang Guo, Zixuan Pang, Cong Gao, Xiulai Chen, Liming Liu
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Hui Liu, Yanli Qi, Pei Zhou, Chao Ye, Cong Gao, Xiulai Chen, Liming Liu
Summary: This review proposes a strategy of microbial physiological engineering (MPE) to improve the efficiency of microbial cell factories, focusing on enhancing substrate utilization, growth performance, stress tolerance, and product export capacity.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yang Li, Shujie Yang, Danlei Ma, Wei Song, Cong Gao, Liming Liu, Xiulai Chen
Summary: Organic acids, as building block compounds, are widely used in various industries with chemical synthesis being the primary method. Microbial cell factories offer a promising approach for sustainable organic acid production but face challenges in cellular metabolism regulation. Metabolic engineering strategies have been developed to reprogram microbial cell factories for higher productivity of organic acids by improving enzyme efficiency, balancing biosynthetic pathways, enhancing metabolic flux, optimizing the metabolic network, and broadening substrate utilization.
NATURAL PRODUCT REPORTS
(2021)