Article
Chemistry, Analytical
Septila Renata, Nitish Verma, Zhijay Tu, Rong-Long Pan, Mario Hofmann, Chun-Hung Lin
Summary: The study developed a tri-functional nanoprobe for detecting sialic acid on cell surfaces with high sensitivity and spatial resolution. The probe utilized two recognition groups and a Raman reporter to enhance the efficiency of sialic acid recognition and capture.
Article
Chemistry, Multidisciplinary
Palash Jana, Sudeep Koppayithodi, Madhukrishnan Murali, Monochura Saha, Kaustabh Kumar Maiti, Subhajit Bandyopadhyay
Summary: A two-component aggregation-induced emissive (AIE) probe has been developed that can interact with SA and other saccharides via noncovalent interactions, enabling reliable detection and clear discrimination. Its potential application in cellular glycan mapping has been explored in breast cancer cells.
Article
Chemistry, Analytical
Jian Lv, Shuai Chang, Xiaoyuan Wang, Zerui Zhou, Binbin Chen, Ruocan Qian, Dawei Li
Summary: The study presents a highly efficient and convenient two-step tagging strategy for live-cell profiling of membrane sialic acids using fluorescence imaging and SERS labeling. By using tumor markers as model proteins, sialic acids can be partitioned into distinct space domains, allowing for classification of various types of cells. This strategy provides a robust and versatile platform for highly detailed analysis of cell surface saccharide profiles, with potential applications in optical biosensing and molecular diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Hyun Soo Kim, Hyun Jung Kim, Jaehun Lee, Taeha Lee, Jongsu Yun, Gyudo Lee, Yoochan Hong
Summary: The paper presents a method for liquid biopsy analysis using SERS technology, including the fabrication of a SERS substrate with silver nanosnowflakes for simultaneous detection of creatinine and cortisol in human sweat.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Kaixin Chen, Haoling Chen, Songxian Liang, Jindan Wu, Ping Zhou, Nan Li
Summary: A new SERS sensor has been developed for background-free detection of H2O2, with high sensitivity and accuracy. By utilizing gold microparticles and specific chemical reactions, this sensor enables reliable quantitative analysis of H2O2 in complex samples.
Article
Chemistry, Physical
Guoqiang Fang, Wuliji Hasi, Xuanyu Sha, Guangxu Cao, Siqingaowa Han, Jinlei Wu, Xiang Lin, Zhouzhou Bao
Summary: Surface-enhanced Raman scattering (SERS) is a highly sensitive tool used in environmental testing. However, detecting and quantifying weakly adsorbed molecules (such as heavy metal ions) remains challenging. In this study, a combination of clean SERS substrates capable of capturing heavy metal ions and convolutional neural network (CNN) algorithm models is used for quantitative detection. The results demonstrate fast and accurate prediction of As5+ ion concentrations, with a determination coefficient of 0.991 between predicted and actual values.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Pei Zeng, Mengjie Zheng, Hao Chen, Guanying Chen, Zhiwen Shu, Lei Chen, Huikang Liang, Yuting Zhou, Qian Zhao, Huigao Duan
Summary: Seeking sensitive, large-scale, and low-cost substrates is crucial for practical applications of SERS technology. Noble metallic plasmonic nanostructures with dense hot spots have received significant attention due to their potential for sensitive and stable SERS performance. This work presents a simple fabrication method to achieve wafer-scale ultradense tilted and staggered plasmonic metallic nanopillars filled with numerous nanogaps. The resulting SERS substrate exhibits excellent detection limit, reproducibility, and long-term stability, making it promising for low-cost and high-performance sensors.
Article
Biochemical Research Methods
Wen Liu, Shana Zhou, Jing Liu, Xin Zhao, Zhe Feng, Dongmei Wang, Zhengjun Gong, Meikun Fan
Summary: A highly reproducible FLM SERS substrate was developed for quantitative detection of 6-TG in human urine and serum, with an optimized Au/Ag ratio of AgcoreAushell NPs. The platform showed stable experimental results with RSD below 10% and a calibration curve with an R-2 value of 0.988 in the range of 10-100 μg kg-1. The LOD of 6-TG was determined to be 5 μg kg-1, making it suitable for rapid and reliable analysis in body fluids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Wonkyoung Lee, Byoung-Hoon Kang, Hyunwoo Yang, Moonseong Park, Ji Hyun Kwak, Taerin Chung, Yong Jeong, Bong Kyu Kim, Ki-Hun Jeong
Summary: The spread spectrum SERS method allows rapid quantification of neurotransmitters with high temporal resolution and signal-to-noise ratio improvement. It enables unlabelled detection of neurotransmitters without Raman reporters, showing potential for early diagnosis of neurological disorders and biosensing applications.
NATURE COMMUNICATIONS
(2021)
Article
Spectroscopy
Fang Mi, Ming Guan, Ying Wang, Guotong Chen, Pengfei Geng, Qiuling Cui, Hongtao Huan
Summary: We present a cost-effective and highly sensitive label-free SERS biosensor for pathogen detection. The biosensor utilizes 4-formylphenylboric acid functionalized magnetic nanoparticles to adsorb pathogenic bacteria and aptamer modified Au@AgNPs as SERS substrate for specific binding. The detection of pathogenic bacteria is achieved using a portable Raman spectrometer and analyzed through principal component analysis (PCA). The SERS detection limit for Staphylococcus aureus is 34 CFU/mL, and the linear range for Escherichia coli is 102-106 CFU/mL.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Tingting Zhang, Gongying Wang, Juan Bao, Chang Liu, Weihua Li, Zhe Kong, Xiuyun Sun, Jiansheng Li, Rui Lu
Summary: A highly sensitive method for detecting histamine using surface-enhanced Raman spectroscopy (SERS) was developed by combining Fe3O4 and Ag-based SERS nanotags. The method showed good sensitivity and reliability in detecting histamine in fish samples.
Article
Chemistry, Physical
C. K. Chung, C. Y. Yu
Summary: A new type of SERS substrate is proposed, which does not require the use of metal nanoparticles. The substrate is fabricated by electrochemically micromachining aluminum foil to create a roughened surface with randomly-distributed 3D over-etched cavities and nanopores of anodized aluminum oxide (AAO). This SERS substrate demonstrates high analytical enhancement and low detection limit, making it suitable for rapid detection of substances such as methylene blue.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Analytical
Qiang Zou, Xinchen Ai, Tao Xue
Summary: In this study, an environmentally friendly and label-free SERS probe was synthesized to detect Fe3+ ions in integrated circuit cleaning solution waste. The probe has high sensitivity and a wide detection range, and has potential applications in evaluating whether the waste meets emission criteria.
MICROCHEMICAL JOURNAL
(2021)
Article
Biophysics
Bing Liu, Shiya Zheng, Hanyu Tang, Qian Liu, Haitao Li, Bingbing Gao, Xiangwei Zhao, Fei Sun
Summary: A highly sensitive method for the analysis of free testosterone (FT) was developed using magnetic nanobeads (MNBs) and gap-enhanced surface enhanced Raman scattering (SERS) nanotags. The proposed method achieves high detection sensitivity and a wider linear dynamic detection range, making it suitable for rapid disease diagnosis and analysis of other substances.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Analytical
Nuo Duan, Yuting Chang, Wenhui Lv, Changxin Li, Chunxia Lu, Zhouping Wang, Shijia Wu
Summary: SiO2@Ag NPs core/shell nanoparticles were synthesized and used to fabricate a SERS sensor for the simultaneous determination of histamine and tyramine. This sensing strategy achieved a low detection limit and showed promising applications in the sensitive detection of BAs in animal-derived foodstuff.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).