Article
Biochemical Research Methods
Shemsu Ligani Fereja, Zhongying Fang, Ping Li, Jinhan Guo, Tadesse Haile Fereja, Wei Chen
Summary: This study successfully synthesized highly orange-red emissive gold nanoclusters using glutathione and applied them for the detection of hemin, showing promising results and potential applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Qian Jia, Ruili Zhang, Haohao Yan, Yanbin Feng, Fang Sun, Zuo Yang, Chaoqiang Qiao, Xiaocheng Mou, Jie Tian, Zhongliang Wang
Summary: Accurate detection of lung metastasis is crucial for cancer treatment and prognosis, but remains challenging in clinical practice. This study presents a strategy to develop a pH-activatable near-infrared fluorescent nanoprobe for precise identification of lung cancer and pulmonary metastasis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Yan Wang, Ying Yan, Xinfa Liu, Changbei Ma
Summary: This study developed a simple and fast method to detect alkaline phosphatase quantitatively, with a LOD of 0.0098 U/L. The turn-off strategy allows for the detection of alkaline phosphatase presence, making it a potential tool for quantitative detection in clinical diagnosis.
Article
Engineering, Environmental
Meng-Ting She, Jia-Wei Yang, Bo-Xin Zheng, Wei Long, Xuan-He Huang, Jun-Ren Luo, Ze-Xin Chen, Ao-Lu Liu, Dong-Peng Cai, Wing-Leung Wong, Yu-Jing Lu
Summary: A series of new mitochondrial-selective fluorescent probes have been designed and synthesized, which exhibit high sensitivity, good selectivity, and high photostability, making them suitable for real-time study of mitochondrial functions in live cells.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Xue Zou, Jie Hu, Huanhuan Zhu, Qiumeng Chen, Zhengjun Gong
Summary: A novel fluorescence turn-off sensing strategy was developed for I- ions detection using CDs/His-AuNCs hybrid probes. The fluorescence of the probes was effectively turned off by I- ions through internal filtration effect. Quantitative analysis of I- ions can be achieved by analyzing the fluorescence quenching degree. The established sensing platform has been successfully applied for the detection of I- ions in practical water samples. This work provides new insight into the determination of anions using fluorescent nanoparticles.
MICROCHEMICAL JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Mayeul Collot, Sonia Pfister, Andrey S. Klymchenko
Summary: In this paper, the latest advances in fluorescent plasma membrane (PM) probes for chemical and biophysical sensing as well as for super-resolution imaging are reviewed.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Jia An, Rubing Chen, Meizhu Chen, Yongqin Hu, Ying Lyu, Yufei Liu
Summary: Ratiometric fluorescent probes constructed in this study showed high accuracy and sensitivity for the detection of Ag+. The fluorescence color of the probes changes as the concentration of Ag+ increases. These probes have the potential to be applied in environmental monitoring and food safety assessment, with the ability for visual detection of Ag+.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Inorganic & Nuclear
Wei Quan, Wenhui Song, Qian Zhang, Huawei Huang, Weiying Lin
Summary: The liver, as the metabolic and detoxification center, plays a vital role in various physiological processes. Normal liver function is crucial for maintaining overall health, while abnormal liver function can lead to liver diseases. Early diagnosis of liver diseases is essential for timely treatment. The use of fluorescent probes for detecting biomarkers of liver diseases has provided valuable insights for pathological studies, drug development, and disease diagnosis and treatment in recent years. This review summarizes the progress of fluorescence probes in detecting hepatopathy-related biomarkers and discusses the current challenges and future directions. The development of long wavelength probes, multi-detection probes, quantitative analysis probes, and multi-modal probes is expected to be a popular trend. This review aims to facilitate the development of novel fluorescent probes for disease detection and provide guidance for diagnosis and treatment.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Tao Li, Yu Zhang, Ziwei Wang, Luolong Qing, Liangjun Zhang, Na Peng, Yi Liu, Silong Zhang, Huan He
Summary: This study presents two water-soluble fluorescent probes based on AIEgen that can monitor the expression of a specific HDAC isoform in living cells. These probes exhibit fast response time, low interference, and are highly attractive for early cancer diagnosis, treatment monitoring, and prognosis assessment.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Medicine, Research & Experimental
Xiaoning Zhang, Shasha Li, Huizhen Ma, Hao Wang, Ruiping Zhang, Xiao-Dong Zhang
Summary: NIR-II imaging technology has made significant progress in diagnosing diseases with its high resolution and deep tissue penetration capabilities. Activatable NIR-II fluorescence probes show great potential in reporting pathological changes, exhibiting tunable properties and excellent biocompatibility.
Article
Nanoscience & Nanotechnology
Sawinee Ngernpimai, Janpen Thonghlueng, Yaowaret Phothikul, Apiwat Chompoosor, Raynoo Thanan, Theerapong Puangmali, Chadamas Sakonsinsiri
Summary: In this study, a highly sensitive fluorescent aptasensor was developed for detecting 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) in urine. The aptasensor used aptamer-mediated quenching of amino-terminated oligo(ethylene glycol)-capped gold nanoparticles (NH2-TEG-AuNPs) towards green emissive carbon dots (G-CDs) as the detection mechanism. The system demonstrated high selectivity and sensitivity over a broad concentration range, and has the potential to be developed as a new platform for rapid screening of 8-oxo-dG.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Medicinal
Morgane Sarrazin, Benjamin P. Martin, Romain Avellan, Giri Raj Gnawali, Isabelle Poncin, Hugo Le Guenno, Christopher D. Spilling, Jean-Francois Cavalier, Stephane Canan
Summary: Patients with cystic fibrosis (CF) are at a higher risk of acquiring non-tuberculous mycobacteria infections, particularly Mycobacterium abscessus. This study provides new insights into the mechanism of action of cyclipostins and cyclophostin analogues against intramacrophagic M. abscessus cells, highlighting the importance of intracellular accumulation and disruption of endolysosomal pH. The fluorescent compounds synthesized in this study may also serve as efficient probes for labeling mycobacterial species.
ACS INFECTIOUS DISEASES
(2022)
Article
Spectroscopy
Linlin Chen, Tianjiao Fan, Wei Li, Jiaqi Song, Jiaxin Zhang, Ling Wang, Ke Han
Summary: A novel and rapid fluorescence method for nitrite determination has been developed by utilizing the interaction between gold nanoparticles and methanobactin (Mb) fluorescence, demonstrating great practical application prospects for nitrite analysis in real samples such as milk, ham sausage, and leaf mustard.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Inorganic & Nuclear
Houzong Yao, Guangyu Zhu
Summary: We present a strategy using a fluorescence turn-on sensor to quantitatively monitor the reduction of platinum(iv) prodrugs in real-time. Proteins with high molecular weights, particularly those between 10 and 100 kDa, play a significant role in the reduction of platinum(iv) complexes in cell extracts.
DALTON TRANSACTIONS
(2022)
Article
Biochemical Research Methods
Ghasem Anbiaee, Zahra Khoshbin, Javad Zamanian, Ali Samie, Mohammad Ramezani, Mona Alibolandi, Noor Mohammd Danesh, Seyed Mohammad Taghdisi, Khalil Abnous
Summary: To rapidly and easily determine the detrimental effects of drugs such as cocaine on the human body, the development of sensing methods is highly necessary. We designed a cost-effective fluorescent aptasensor for cocaine detection using the signal enhancement capability of the UiO-66/AuNPs nanocomposite. The aptasensor achieved a linear response range of 0.5 & mu;M-20 & mu;M with a low detection limit of 0.178 & mu;M. This method has substantial benefits for the development of a low-cost and facile tool in medicine and forensic science.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Cell Biology
Ching Ying Huang, Martin W. Nicholson, Jyun Yuan Wang, Chien Yu Ting, Ming Heng Tsai, Yu Che Cheng, Chun Lin Liu, Darien Z. H. Chan, Yi Chan Lee, Ching Chuan Hsu, Yu Hung Hsu, Chiou Fong Yang, Cindy M. C. Chang, Shu Chian Ruan, Po Ju Lin, Jen Hao Lin, Li Lun Chen, Marvin L. Hsieh, Yuan Yuan Cheng, Wan Tseng Hsu, Yi Ling Lin, Chien Hsiun Chen, Yu Hsiang Hsu, Ying Ta Wu, Timothy A. Hacker, Joseph C. Wu, Timothy J. Kamp, Patrick C. H. Hsieh
Summary: In this study, a population-based hiPSC drug screening platform is established to assess toxicity. Thirteen HLA-homozygous super donors are identified after recruiting 1,000 healthy donors and screening for high-frequency HLA haplotypes, representing at least 477,611,135 of the global population. The differentiated hiPSCs into cardiomyocytes and neurons demonstrate their utility in a high-throughput toxicity screen. Dose-dependent toxicity and functional modulation of hit compounds are validated, and reproducible in vivo drug toxicity results using mouse models are shown. This study shows the feasibility of using a population-based hiPSC drug screening platform to study inter population differences in drug toxicity and adverse drug reactions.
Article
Chemistry, Analytical
Wei-Jhen Wang, Min-Chuan Chou, Ya-Jane Lee, Wei-Li Hsu, Gou-Jen Wang
Summary: A novel electrochemical immunosensor based on a gold nanoparticle electrode was proposed for accurate detection of NGAL in cat urine samples. The sensing scheme demonstrated a wide detection range and low limit of detection, enabling the differentiation of kidney health status.
Article
Chemistry, Multidisciplinary
Cheng-Chung Chang, Chia-Feng Hsieh, Hsing-Ju Wu, Mohamed Ameen, Tun-Pin Hung
Summary: Sonodynamic therapy (SDT) offers a noninvasive approach for tumor treatment by using ultrasound to activate a sonosensitizer. Two synthesized phenothiazine derivatives showed potential as photosensitizers and sonosensitizers. Among them, compound MB6C exhibited superior therapeutic effects and could be a potential candidate for photodynamic therapy (PDT) and SDT.
APPLIED SCIENCES-BASEL
(2022)
Article
Pharmacology & Pharmacy
Yue-Zhi Lee, Hsing-Yu Hsu, Cheng-Wei Yang, Yi-Ling Lin, Sui-Yuan Chang, Ruey-Bing Yang, Jian-Jong Liang, Tai-Ling Chao, Chun-Che Liao, Han-Chieh Kao, Jang-Yang Chang, Huey-Kang Sytwu, Chiung-Tong Chen, Shiow-Ju Lee
Summary: Ciclesonide blocks coronavirus-induced cytokine production by increasing I kappa B alpha protein levels and decreasing p65 nuclear translocation, and it can be used in the treatment of COVID-19 patients. The combination of Ciclesonide and dbq33b synergistically decreases cytokine levels and inhibits coronavirus replication. Therefore, this combination therapy is a promising treatment option for COVID-19 patients with high viral loads and NF-kappa B-mediated cytokine storms.
Article
Chemistry, Medicinal
Martin W. Nicholson, Ching-Ying Huang, Jyun-Yuan Wang, Chien-Yu Ting, Yu-Che Cheng, Darien Z. H. Chan, Yi-Chan Lee, Ching-Chuan Hsu, Yu-Hung Hsu, Cindy M. C. Chang, Marvin L. Hsieh, Yuan-Yuan Cheng, Yi-Ling Lin, Chien-Hsiun Chen, Ying-Ta Wu, Timothy A. Hacker, Joseph C. Wu, Timothy J. Kamp, Patrick C. H. Hsieh
Summary: This study utilized a drug screening platform based on human induced pluripotent stem cells to evaluate the cardiotoxicity and neurotoxicity of anti-COVID-19 drugs. The researchers found that certain drugs displayed cardiotoxic or neurotoxic effects, providing valuable information for clinicians when choosing treatment options.
Article
Biology
Wei-Chun Tang, Yen-Ting Liu, Cheng-Han Yeh, Chieh-Han Lu, Chiao-Hui Tu, Yi-Ling Lin, Yu-Chun Lin, Tsui-Ling Hsu, Liang Gao, Shu-Wei Chang, Peilin Chen, Bi-Chang Chen
Summary: This study improves lattice lightsheet microscopy (LLSM) to manipulate cellular behavior with subcellular resolution through optogenetic activation (optoLLSM). By integrating a position-controllable Bessel beam as a stimulation source, spatiotemporal photoactivation can be achieved by changing spatial light modulator (SLM) patterns. The study demonstrates the ability to control membrane ruffling and cell migration behavior using light-triggered recruitment of phosphoinositide 3-kinase and guided cell migration with low energy power.
COMMUNICATIONS BIOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Po-Min Chung, Ying-Ting Lin, Gou-Jen Wang
Summary: An Au/Ni nanopillar microarray electrode was developed for detecting red blood cell (RBC) concentrations. The electrode was fabricated using AAO as the template and had a diameter of 50 µm. The detection scheme showed two linear detection ranges and high recovery rates in whole blood sample tests, indicating its feasibility for RBC concentration detection in real blood samples.
IEEE SENSORS JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Ying-Ting Lin, Yen-Ting Tung, Jhen-Yuan Wong, Gou-Jen Wang
Summary: To study biochemical and mechanical properties, it is necessary to engineer customized capillary networks that match the characteristics of different organs. A scaffold-wrapping approach was used to create a perfusable capillary network by culturing human umbilical vein endothelial cells on a patterned fibrin scaffold and encapsulating it in collagen gel to form a vascular lumen. The dimensions of the capillary network could be controlled at the micrometer level.
MATERIALS & DESIGN
(2023)
Correction
Pharmacology & Pharmacy
Yue-Zhi Lee, Hsing-Yu Hsu, Cheng-Wei Yang, Yi-Ling Lin, Sui-Yuan Chang, Ruey-Bing Yang, Jian-Jong Liang, Tai-Ling Chao, Chun-Che Liao, Han-Chieh Kao, Jang-Yang Chang, Huey-Kang Sytwu, Chiung-Tong Chen, Shiow-Ju Lee
Article
Biochemistry & Molecular Biology
Amal Farghal Noreldein Elsyed, Gah-Lai Wong, Mohamed Ameen, Min-Wei Wu, Cheng-Chung Chang
Summary: Chemical structures with aggregation-induced emission enhancement (AIEE) and intramolecular charge transfer (ICT) properties have attracted the attention of researchers. In this study, we designed and synthesized a series of fluorophores with tunable AIEE and ICT properties by adjusting the medium polarity. We investigated the optical properties and self-assembly abilities of these molecules and observed different nanostructures and spectral changes by adjusting the water ratio in mixed solutions. The results show that the AIEE comes from the formation of micelle-like nanoaggregates, leading to a blue-shift in emission and enhanced intensity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Hui-Ying Ko, Yao-Tsun Li, Ya-Yuan Li, Ming-Tsai Chiang, Yi-Ling Lee, Wen-Chun Liu, Chun-Che Liao, Chih-Shin Chang, Yi-Ling Lin
Summary: COVID-19 is a major global public health threat, and RT-qPCR is used for viral RNA detection. Saliva has been suggested as a practical alternative to nasopharyngeal swabs for viral RNA detection. This study evaluated the effect of saliva on viral RNA detection and found that saliva increased the C-T values of SARS-CoV-2 RNA samples compared to viral transport medium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Electrical & Electronic
Chun-Chiang Tseng, Ying-Ting Lin, Gou-Jen Wang
Summary: This study developed a novel electrochemical glycosylated hemoglobin (HbA1c) immunosensor based on a nanostructured electrode. The HbA1c antibodies were successfully immobilized on the electrode, and the sensor showed a linear detection range from 2500 to 10,000 ng/mL with a low limit of detection of 2253 ng/mL. The developed HbA1c-sensing chip offers simple operation, low cost, short detection time, and high feasibility for clinical application.
IEEE SENSORS JOURNAL
(2023)
Article
Physics, Applied
Smruti R. Sahoo, Chun-Ta Huang, Kunju Tsai, Gou-Jen Wang, Cheng-Chung Chang
Summary: In this study, a single-component, multiscale, three-dimensional SERS substrate was fabricated using silver nanowires packing. The substrate exhibited strong Raman enhancement effect and isotropic localized surface plasmon resonance. Experimental results showed that the substrate had high efficiency and sensitivity for detecting SARS-CoV-2-related biomolecules.
APPLIED PHYSICS REVIEWS
(2023)
Article
Cell Biology
I-Jung Lee, Cheng-Pu Sun, Ping-Yi Wu, Yu-Hua Lan, I-Hsuan Wang, Wen-Chun Liu, Joyce Pei-Yi Yuan, Yu-Wei Chang, Sheng-Che Tseng, Szu- Tsung, Yu-Chi Chou, Monika Kumari, Yin-Shiou Lin, Hui-Feng Chen, Tsung-Yen Chen, Chih-Chao Lin, Chi-Wen Chiu, Chung-Hsuan Hsieh, Cheng-Ying Chuang, Chao-Min Cheng, Hsiu-Ting Lin, Wan-Yu Chen, Fu-Fei Hsu, Ming-Hsiang Hong, Chun-Che Liao, Chih-Shin Chang, Jian-Jong Liang, Hsiu-Hua Ma, Ming-Tsai Chiang, Hsin-Ni Liao, Hui-Ying Ko, Liang-Yu Chen, Yi-An Ko, Pei-Yu Yu, Tzu-Jing Yang, Po-Cheng Chiang, Shang-Te Hsu, Yi-Ling Lin, Chong-Chou Lee, Han-Chung Wu, Mi-Hua Tao
Summary: This study reports an mRNA-based vaccine using an engineered hybrid receptor binding domain that can generate neutralizing antibodies and T cell responses against various SARS-CoV-2 variants. The results demonstrate that including different antigenic mutations from different variants is a feasible approach to develop vaccines with broad protection.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Medicine, Research & Experimental
Chang Yi Wang, Kao-Pin Hwang, Hui-Kai Kuo, Wen-Jiun Peng, Yea-Huei Shen, Be-Sheng Kuo, Juin-Hua Huang, Hope Liu, Yu-Hsin Ho, Feng Lin, Shuang Ding, Zhi Liu, Huan-Ting Wu, Ching-Tai Huang, Yuarn-Jang Lee, Ming-Che Liu, Yi-Ching Yang, Po-Liang Lu, Hung-Chin Tsai, Chen-Hsiang Lee, Zhi-Yuan Shi, Chun-Eng Liu, Chun-Hsing Liao, Feng-Yee Chang, Hsiang-Cheng Chen, Fu-Der Wang, Kuo-Liang Hou, Jennifer Cheng, Min-Sheng Wang, Ya-Ting Yang, Han-Chen Chiu, Ming-Han Jiang, Hao-Yu Shih, Hsuan-Yu Shen, Po-Yen Chang, Yu-Rou Lan, Chi-Tian Chen, Yi-Ling Lin, Jian-Jong Liang, Chun-Che Liao, Yu-Chi Chou, Mary Kate Morris, Carl V. Hanson, Farshad Guirakhoo, Michael Hellerstein, Hui-Jing Yu, Chwan-Chuen King, Tracy Kemp, D. Gray Heppner, Thomas P. Monath
Summary: The UB-612 vaccine showed favorable safety profile, long-lasting antibody response, and broad T cell immunity against SARS-CoV-2 variants, including Delta and Omicron, in phase I/II trials.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)