期刊
BIOSENSORS & BIOELECTRONICS
卷 79, 期 -, 页码 320-326出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.056
关键词
Gold nanoparticles; Reduced graphene oxide; Mercury ion; Thymine-Hg2+-Thymine; Electrochemical sensor
类别
资金
- National Research Foundation for the Doctoral Program of Higher Education of China [20120131110009]
- Fundamental Research Funds of Shandong University [2015HW019]
- National Natural Science Foundation of China [21373129]
A sensitive, selective and reusable electrochemical biosensor for the determination of mercury ions (Hg2+) has been developed based on thymine (T) modified gold nanoparticles/reduced graphene oxide (AuNPs/rGO) nanocomposites. Graphene oxide (GO) was electrochemically reduced on a glassy carbon substrate. Subsequently, AuNPs were deposited onto the surface of rGO by cyclic voltammetry. For functionalization of the electrode, the carboxylic group of the thymine-1-acetic acid was covalently coupled with the amine group of the cysteamine which self-assembled onto AuNPs. The structural features of the T bases functionalized AuNPs/rGO electrode were confirmed by attenuated total reflection infrared (ATR-IR) spectroscopy and scanning electron microscopy (SEM) spectroscopy. Each step of the modification process was characterized by cyclic voltammetry (CV) and electrochemical impedence spectroscopy (EIS). The T bases modified AuNPs/rGO electrode was applied to detect various trace metal ions by differential pulse voltammetry (DPV). The proposed biosensor was found to be highly sensitive to Hg2+ in the range of 10 ng/L-1.0 mu g/L. The biosensor afforded excellent selectivity for Hg2+ against other heavy metal ions such as Zn2+, Cd2+, Pb2+, Cu2+, NP+, and Co2+. Furthermore, the developed sensor exhibited a high reusability through a simple washing, In addition, the prepared biosensor was successfully applied to assay Hg2+ in real environmental samples. (C) 2015 Elsevier B.V. All rights reserved.
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