期刊
BIOSENSORS & BIOELECTRONICS
卷 79, 期 -, 页码 29-33出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.012
关键词
Human alpha-thrombin; G-quadruplex DNAzyme; Exonuclease III; Hairpin assembly; Chemiluminescence
类别
资金
- Social Development Fund of Jiangsu Province [BE2013615]
Detection of ultralow concentration of specific protein plays a key role in biotechnological applications and medical diagnostics. In this study, we demonstrate an amplified chemiluminescence biosensing platform for sensitive detection of protein. The biosensing platform ingeniously combines target-catalyzed hairpin assembly and Exo III-assisted signal amplification. A catalytic G-quadruplex-hemin DNAzyme is further employed to stimulate the generation of chemiluminescence in the presence of luminol and H2O2. These designs together allow a high sensitivity for the biotarget, human alpha-thrombin, resulting in a detection limit of 0.92 pM, and it is much lower than previous reported studies. In addition, the proposed biosensing platform is versatile. By conjugating with various recognition units, it could be employed to sensitive detect various DNA-binding proteins and might find wide applications in biomedical fields. (C) 2015 Elsevier B.V. All rights reserved.
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