Article
Chemistry, Analytical
Xiaolin Gao, Zhicong Sun, Xiaoyang Wang, Wanqi Zhang, Yue Wang, Jie Han, Xia Sun, Yemin Guo, Falan Li, Shicai Xu
Summary: A ratiometric electrochemical aptasensor was developed using Fc-labeled primers and GDY-MB nanocomposite for high-precision detection of KAN. The aptasensor exhibited strong stability, specificity, and successful detection of KAN in real milk samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Chengli Zhang, Xun You, Yuliang Li, Yuanyuan Zuo, Wei Wang, DanDan Li, ShengWei Huang, Hangzhi Hu, Feng Yuan, Fuqiang Shao, Minggang Yin
Summary: In this study, an aptamer-based amplification strategy using Ce-MOF and m-PdNFs-G4-MBs nanocomposite was developed for the sensitive detection of dopamine (DA). The results showed that the new detection platform achieved a minimum detection limit of 6 pmol/L for DA with good specificity. This platform has the potential to provide a convenient and efficient tool for the diagnosis of multiple neurological disorders in the future.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Dong Liu, Shuyun Meng, Xiuli Shen, Yuye Li, Xiaohong Yan, Tianyan You
Summary: This work presents a dual-ratiometric aptasensor for streptomycin detection, utilizing the in-situ coupling of photoelectrochemical and electrochemical assays. The use of methylene blue as a bifunctional probe enables the simultaneous generation of photocurrent and redox current, resulting in enhanced analytical performances compared to single-signal or single-ratiometric assays. The MB-based dual-mode sensing offers a promising tool for studying interactions between PEC and EC behaviors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Laszlo Kekedy-Nagy, James M. Perry, Samuel R. Little, Oriol Y. Llorens, Steve C. C. Shih
Summary: We have developed a new on-off, cost-effective, and rapid electrochemical aptasensor combined with a microfluidics cartridge system for the detection of Delta(9)-THC in human saliva. The sensor showed a high sensitivity to Delta(9)-THC and can detect it at a concentration as low as 1 nM in PBS. However, the presence of raw human saliva significantly increased the limit of detection due to interfering elements. With saliva filtration, the limit of detection improved to 5 nM, which is lower than the concentrations associated with impairment.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Zhiguang Suo, Xingyuan Niu, Ruike Liu, Lingkun Xin, Yong Liu, Min Wei
Summary: In this study, a ratiometric electrochemical aptasensor using methylene blue and Ag+ dual-signal probes, and incorporating Au@Pt/Fe-N-C nanocomposites-induced signal amplification, was developed for rapid detection of Zearalenone. The aptasensor exhibited excellent sensitivity and accuracy, and successfully detected Zearalenone in corn flour samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
N. Salandari-Jolge, Ali A. Ensafi, B. Rezaei
Summary: An electrochemical aptasensor based on a single-stranded aptamer-Au@Fe-MIL-88 complex was developed for the sensitive and selective determination of insulin. The sensor demonstrated good sensitivity and reproducibility for detecting insulin in serum samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Lijun Luo, Xiaohong Liu, Xiaoya Bi, Libo Li, Tianyan You
Summary: In this study, the effects of methylene blue (MB) on the lumiphore and co-reactant of self-enhanced electrochemiluminescence (ECL) composites were investigated for the first time. The dual-quenching effects of MB on the ECL signal were ingeniously integrated and designed in a ratiometric aptasensor for zearalenone detection, significantly enhancing the sensitivity. This innovative ratiometric aptasensor achieved a wide linear range and a low detection limit, broadening the application in sensitive and precise analysis of mycotoxins.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Xiaoping Tan, Hongxing Yang, Xin Ran, Zhi Li, Lianpeng Zhang, Wei Gao, Xiaojian Zhou, Guanben Du, Long Yang
Summary: In this study, a CP6-AuNP-functionalized CNC nanohybrid with high electrocatalytic activity and excellent selective molecular recognition capability for BPA detection was prepared. The host-guest interactions were studied through fluorescence titration experiments, revealing a high binding constant between CP6 and BPA. The electrochemical sensing method showed satisfactory results for detecting BPA in tap water and milk samples.
NEW JOURNAL OF CHEMISTRY
(2021)
Review
Chemistry, Analytical
Qian Liu, Xinmin Yue, Yixian Li, Fang Wu, Meng Meng, Yongmei Yin, Rimo Xi
Summary: The study introduces a novel electrochemical aptasensor based on specific host-guest interactions between cucurbit [7]uril and ferrocene, providing high selectivity and sensitivity in detecting exosomes. The platform allows for specific capture and release of exosomes, showing good performance in exosome detection while maintaining their integrity. This approach can potentially be applied to other target biomolecules by altering the recognition pairs.
Review
Chemistry, Analytical
Hossein Khosropour, Pramod K. Kalambate, Rupali P. Kalambate, Khageephun Permpoka, Xiaohong Zhou, George Y. Chen, Wanida Laiwattanapaisal
Summary: There has been a rise in pesticide use as a result of agricultural industrialization, leading to health and environmental hazards. Therefore, rapid and reliable detection of harmful organophosphorus toxins is of utmost importance. Aptasensors, as a new type of biosensor, exhibit exceptional recognition capability and flexibility. Recent research has shown that nanotechnology can enhance the sensitivity and accuracy of aptasensors.
Article
Polymer Science
Wenhui Zhu, Caiyun Zhang, Yali Chen, Qiliang Deng
Summary: A novel ferrocene-containing polymer with both magnetism and photothermal properties was synthesized in this study. The polymer showed high photo-thermocatalytic activity, with a degradation rate of 99.5% for methylene blue under an 808 nm laser within 15 minutes. These findings suggest a promising approach for degrading organic pollutants using this polymer.
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Chemistry, Analytical
Zhiping Song, Juan Song, Feng Gao, Xiaoping Chen, Qinghua Wang, Yanan Zhao, Xuguang Huang, Chaoyong Yang, Qingxiang Wang
Summary: In this study, a novel electrochemical aptasensor was developed for the rapid and accurate determination of cardiac troponin I. The sensor exhibited ultra-low detection limit and a wide linear range.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Zhiping Song, Juan Song, Feng Gao, Xiaoping Chen, Qinghua Wang, Yanan Zhao, Xuguang Huang, Chaoyong Yang, Qingxiang Wang
Summary: In this study, an electrochemical aptasensor for the detection of cardiac troponin I was developed by utilizing the physical and electrochemical properties of ferrocene-based covalent organic framework nanosheets. The sensor exhibited ultra-low detection limit and a wide linear range, demonstrating excellent performance in practical applications, such as serum assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xiaoying Wang, Linyu Li, Xuan Gu, Bingjia Yu, Meng Jiang
Summary: An electrochemical aptasensor has been proposed for sensitive quantification of amyloid-beta oligomers (A beta Os), with excellent selectivity and sensitivity in detecting these neurotoxic substances related to Alzheimer's disease (AD). The switchable sensor exhibited a linear range from 0.5 fM to 500 fM and a detection limit of 0.25 fM, showing promising potential for AD diagnosis and clinical applications.
Article
Chemistry, Multidisciplinary
Muhan Deng, Changhan Liao, Xiufeng Wang, Shangda Chen, Fugang Qi, Xueliang Zhao, Peng Yu
CANADIAN JOURNAL OF CHEMISTRY
(2019)
Article
Chemistry, Analytical
Peng Yu, Muhan Deng, Yi Yang
Article
Chemistry, Analytical
Erhu Xiong, Deshuai Zhen, Ling Jiang, Xiaoming Zhou
ANALYTICAL CHEMISTRY
(2019)
Article
Materials Science, Multidisciplinary
Hong Yu, Chun Peng, Fa-Cai Li, Peng Yu
MATERIALS RESEARCH EXPRESS
(2019)
Article
Chemistry, Multidisciplinary
Menglu Hu, Chaoqun Yuan, Tian Tian, Xusheng Wang, Jian Sun, Erhu Xiong, Xiaoming Zhou
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Analytical
Peng Yu, Muhan Deng, Yi Yang, Beixi Nie, Shaoyu Zhao
Article
Chemistry, Multidisciplinary
Huahua Yue, Mengqi Huang, Tian Tian, Erhu Xiong, Xiaoming Zhou
Summary: CRISPR-based diagnoses, derived from gene-editing technology, have unique features such as recognition at physiological temperature, excellent specificity, and high-efficiency signal amplification capabilities. Micro/nanotechnologies play key roles in the advancement of CRISPR diagnosis, including the use of nanoprobes, nanochips, microfluidic technology, and lateral flow strips, leading to new opportunities and improvements such as equipment-free detection, more compact detection systems, improved sensitivity, and quantitative capabilities. Despite significant progress, CRISPR diagnosis has not yet reached its full potential, with upcoming opportunities and improvements expected to be driven by micro/nanotechnologies.
Article
Chemistry, Multidisciplinary
Huahua Yue, Bowen Shu, Tian Tian, Erhu Xiong, Mengqi Huang, Debin Zhu, Jian Sun, Qing Liu, Shichan Wang, Yirong Li, Xiaoming Zhou
Summary: This study presents a CRISPR-Cas12a-based molecular diagnostic technique for amplification-free and absolute quantification of DNA at the single-molecule level, achieving high sensitivity and specificity through optimized reaction parameters and microdroplet technology. The method can directly count diverse viruses' DNAs and demonstrate versatility and quantification capability, showing potential as a versatile and quantitative platform for molecular diagnosis.
Article
Chemistry, Analytical
Mei Lin, Huahua Yue, Tian Tian, Erhu Xiong, Debin Zhu, Yongzhong Jiang, Xiaoming Zhou
Summary: A glycerol-enhanced one-pot RPA-CRISPR/Cas12a method has been discovered to greatly improve the detection efficiency, making it comparable to the conventional two-step method. The enhanced efficiency is attributed to the phase separation of the RPA and CRISPR/Cas12a system caused by glycerol viscosity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ling Jiang, Jinlian Du, Haili Xu, Xiaohua Zhuo, Jinlong Ai, Jiayu Zeng, Ronghua Yang, Erhu Xiong
Summary: In this study, a novel CRISPR/Cas13a-mediated photoelectrochemical biosensor was proposed for the direct assay of miRNA-21, which showed improved specificity and sensitivity compared to traditional nucleic acid-based strategies. The CRISPR/Cas13a system accurately recognized and efficiently cleaved the target, without the need for complex nucleic acid sequence design. This biosensor demonstrated excellent analysis capability and stability, suggesting promising application prospects in early disease diagnosis and biomedical research.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Beixi Nie, Shaoyu Zhao, Muhan Deng, Peng Yu, Yi Yang, Weixin Lei, Lu Yin
Summary: This study integrated single-layered paint-spraying paper based microfluidic devices with colorimetric assay for the first time. By using PMMA masks and spray paint technology to construct hydrophobic barriers, the fabricated devices were successfully applied in colorimetric assays for single or multiple analytes.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Tao Lei, Ting Huang, Tianze Wang, Peng Yu, Taiping Qing, Beixi Nie
NEW JOURNAL OF CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Guan-Li Xie, Hong Yu, Mu-Han Deng, Xue-Liang Zhao, Peng Yu
Article
Chemistry, Analytical
Erhu Xiong, Ling Jiang
Article
Chemistry, Multidisciplinary
Erhu Xiong, Deshuai Zhen, Ling Jiang
CHEMICAL COMMUNICATIONS
(2018)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)