期刊
BIOSENSORS & BIOELECTRONICS
卷 79, 期 -, 页码 669-678出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.086
关键词
Field-effect transistors; Potentiometric biosensors; Immunosensors; Serological diagnostics; Bovine Respiratory Disease; Bovine Herpes Virus-1
类别
资金
- National Science Foundation (NSF), through CBET Award [1264705]
- Swiss National Science Foundation (SNSF) [P2BSP2_148636, P300P2_158502]
- Science Foundation Ireland (SFI) under US-Ireland Programme [SFI12/US/I2476]
- Department for Employment and Learning, Northern Ireland (DELHI) [USI 039]
- Center for Low Energy Systems Technology
- MARCO
- DARPA
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1264705] Funding Source: National Science Foundation
- Swiss National Science Foundation (SNF) [P2BSP2_148636, P300P2_158502] Funding Source: Swiss National Science Foundation (SNF)
Quantitative point-of-care (POC) devices are the next generation for serological disease diagnosis. Whilst pathogen serology is typically performed by centralized laboratories using Enzyme-Linked ImmunoSorbent Assay (ELISA), faster on-site diagnosis would infer improved disease management and treatment decisions. Using the model pathogen Bovine Herpes Virus-1 (BHV-1) this study employs an extended-gate field-effect transistor (FET) for direct potentiometric serological diagnosis. BHV-1 is a major viral pathogen of Bovine Respiratory Disease (BRD), the leading cause of economic loss ($2 billion annually in the US only) to the cattle and dairy industry. To demonstrate the sensor capabilities as a diagnostic tool, BHV-1 viral protein gE was expressed and immobilized on the sensor surface to serve as a capture antigen for a BHV-1-specific antibody (anti-gE), produced in cattle in response to viral infection. The gE-coated immunosensor was shown to be highly sensitive and selective to anti-gE present in commercially available anti-BHV-1 antiserum and in real serum samples from cattle with results being in excellent agreement with Surface Plasmon Resonance (SPR) and ELISA. The FET sensor is significantly faster than ELISA ( < 10 min), a crucial factor for successful disease intervention. This sensor technology is versatile, amenable to multiplexing, easily integrated to POC devices, and has the potential to impact a wide range of human and animal diseases. (C) 2015 Elsevier B.V. All rights reserved.
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