期刊
BIOSENSORS & BIOELECTRONICS
卷 81, 期 -, 页码 15-22出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.096
关键词
Lead ion; Aptasensor; Graphene; Thionine
类别
资金
- National Natural Science Foundation of China [21275127]
- Program for New Century Excellent Talents in Fujian Province University [JA12204]
A label-free and highly sensitive electrochemical aptasensor for Pb2+ was constructed using thionine (TH) as the signaling molecule and graphene (GR) as the signal-enhancing platform. The electrochemical sensing interface was fabricated by stepwise assembly of GR and TH on the lead (II) specific aptamer (LSA) modified electrode. Upon interaction with Pb2+, the aptamer probe on the sensor underwent conformational switch from a single-stranded DNA form to the G-quadruplex structure, causing the GR with assembled TH released from the electrode surface into solution. As a result, the electrochemical signal of TH on the aptasensor was substantially reduced. Under the optimal experimental conditions, the attenuation of peak currents presented a good linear relationship with the logarithm of Pb2+ concentrations over the range from 1.6 x 10(-13) to 1.6 x 10(-10) M. The detection limit was estimated to be 3.2 x 10(-14) M. The aptasensor also exhibited good regenerability, excellent selectivity, and acceptable reproducibility, indicating promising application in environment monitoring of lead. (C) 2016 Elsevier B.V. All rights reserved.
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