Article
Biotechnology & Applied Microbiology
Yue Shan, Weili Yu, Lijuan Shen, Xuan Guo, He Zheng, Jinyi Zhong, Tao Hu, Yinglun Han
Summary: In this study, haloalkane dehalogenase DhaA was covalently conjugated with inulin, a hydrophilic polysaccharide, to enhance the environmental stability of the enzyme. The conjugated inulin formed a hydration layer around DhaA, increasing conformational rigidity and decreasing enzyme entropy. Despite slight changes in structure and enzymatic activity, inulin conjugation delayed structural unfolding and protonation of DhaA in unfavorable conditions. As a result, the environmental stability of DhaA was significantly improved.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Physical
Jianxiong Chen, Xiaodong Ming, Zitao Guo, Yi Shi, Moying Li, Zhongpeng Guo, Yu Xin, Zhenghua Gu, Liang Zhang, Xuan Guo
Summary: In this study, an iron-based hybrid nanocomposite biocatalyst FeHN@DhaA was successfully constructed to enhance the environmental tolerability of DhaA. FeHN@DhaA exhibited excellent immobilized enzymatic properties, better tolerance to extreme acid and alkali, and satisfactory reusability.
Review
Chemistry, Analytical
Elicia L. S. Wong, Khuong Q. Vuong, Edith Chow
Summary: Nanozymes are advanced nanomaterials that mimic natural enzymes and are ideal candidates for real-time and remote environmental monitoring and remediation due to their better structural stability. Through innovative nano-architectural design and transduction methodologies, Nanozymes have made significant progress in detecting heavy metal ions, pesticides, and organic pollutants. Strategies for remediating persistent organic pollutants are also discussed in this review.
Review
Chemistry, Analytical
Miaomiao Wang, Ping Zhu, Shuge Liu, Yating Chen, Dongxin Liang, Yage Liu, Wei Chen, Liping Du, Chunsheng Wu
Summary: Nanozymes are nanomaterials that possess the enzyme-like catalytic functions of natural enzymes as well as the unique properties of nanomaterials. They have gained increasing attention in environmental science and life sciences due to their catalytic activity, stability, tunability, recyclability, and versatility. This review focuses on the recent applications of nanozymes in environmental monitoring, management, and protection. The tuning of nanozymes' catalytic activity based on factors like size, shape, composition, doping, and surface coating is introduced, followed by detailed discussions on their applications in detecting inorganic ions, molecules, organics, and foodborne pathogenic bacteria, as well as their involvement in the degradation of phenolic compounds, dyes, and antibiotics. Additionally, the potential of nanozymes in air purification, biofuel cell construction, and marine antibacterial fouling removal is highlighted. The review concludes with a discussion on the current challenges and future trends of nanozymes in environmental fields.
Article
Energy & Fuels
Giovanni Battista Ariemma, Giancarlo Sorrentino, Mara de Joannon, Pino Sabia, Antonio Albano, Raffaele Ragucci
Summary: Sustainable thermochemical conversion processes require new monitoring and control strategies based on suitable diagnostics methodologies. This involves rethinking combustion diagnostics strategies and utilizing a large number of strategically distributed sensors in the combustion chamber for innovative diagnostics.
Review
Chemistry, Multidisciplinary
Subhi Gupta, Nancy George, Mohini Yadav, Vagish Dwibedi
Summary: Heavy metal pollution has adverse effects on humans and the environment, so a targeted sensing approach for heavy metal ions is needed. Functionalized gold nanoparticles serve as a foundation for sensitive, selective, and analytical biosensing instruments. Functionalization with biomolecules offers biocompatibility, stability, enhanced catalytic activity, low toxicity, and selective binding and sensing to gold nanoparticles. Bio-functionalized gold nanoparticle-based sensors have practical applications in environmental remediation. This review discusses the new advancements in nano-biosensors using bio-functionalized gold nanoparticles for heavy metal ion sensing.
Review
Chemistry, Multidisciplinary
Kai Yan, Padmanathan Karthick Kannan, Dulyawat Doonyapisut, Kangbing Wu, Chan-Hwa Chung, Jingdong Zhang
Summary: Functional materials with attractive electronic and photoelectronic properties have great potential for environmental pollutant monitoring. Rational design and basic signaling strategies are crucial for the development of electrochemical and photoelectrochemical sensors using these materials. Future research in this field should address critical challenges and explore the diverse applications of various functional materials in environmental monitoring.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Chemistry, Analytical
Fengnian Zhao, Li Wang, Mengyue Li, Min Wang, Guangyang Liu, Jianfeng Ping
Summary: This review summarizes the research progress of nanozyme-based biosensors for OP detection in the last six years (2018-2023), discussing the structural classification, catalytic activity, and biosensing strategy. It emphatically introduces the practical applications of nanozyme-enabled biosensors in the direction of simple operation, low cost, and on-site detection, such as optical and electrochemical biosensors. Finally, it prospects the main challenges and future trends, providing theoretical references for nanozyme-based biosensors in OP detection.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Melissa E. King, Chuntao Wang, Maria V. Fonseca Guzman, Michael B. Ross
Summary: This article introduces the material, chemical, and environmental considerations for using plasmonic nanostructures and technologies to degrade environmental pollutants. It particularly focuses on the material considerations for persistent and emerging recalcitrant pollutants. Finally, specific barriers to realizing scalable, efficient, and reliable plasmon-enhanced technology are discussed.
Review
Engineering, Chemical
Reyna Berenice Gonzalez-Gonzalez, Elda A. Flores-Contreras, Everardo Gonzalez-Gonzalez, Nora E. Torres Castillo, Roberto Parra-Saldivar, Hafiz M. N. Iqbal
Summary: Water and soil contamination is a global environmental concern that affects food and water security. Biosensors have shown effectiveness in accurate, on-site, and real-time detection of various pollutants. Different bioreceptors such as cells, antibodies, aptamers, enzymes, and nanozymes have been explored, exhibiting excellent performance. This review discusses the recent advances in each type of biosensor and compares different strategies and challenges for detecting pollutants.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Judith Camalo Vila, Nerea Castro-Aguirre, Gerardo A. Lopez-Munoz, Ainhoa Ferret-Minana, Francesco De Chiara, Javier Ramon-Azcon
Summary: Optical biosensors based on plasmonic nanomaterials have attracted significant scientific interest for their sensitivity, directness, and potential for multiplexing. A pilot study was conducted using optical discs as nanotemplates to develop an integrated plasmonic biosensor for monitoring cell adhesion and growth, showing promising results for real-time analysis of living cells behavior and culture conditions. This approach could offer a non-destructive and reliable tool for assessing cell viability and growth with low variability between technical replicates, improving experimental replicability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Construction & Building Technology
Botao Zhong, Jiadong Guo, Lu Zhang, Haitao Wu, Heng Li, Yuhang Wang
Summary: This study proposes a blockchain-enabled framework for on-site construction environmental monitoring (OCEM), where pollutant data is collected through sensors and monitored automatically using smart contracts. The results show that blockchain can provide trustworthy environmental data and enable continuous monitoring for OCEM.
BUILDING AND ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Narsingh R. Nirala, Jimmy Asiku, Hay Dvir, Giorgi Shtenberg
Summary: In this study, a modified sensing approach using surface-enhanced Raman spectroscopy (SERS) was proposed for rapid and reliable determination of NAG activity in complex media. The sensitivity of the SERS substrates was evaluated and found to be able to successfully recognize NAG in biological fluids.
Article
Chemistry, Analytical
Rachel Salama, Sofia Arshavsky-Graham, Osnat Sella-Tavor, Naama Massad-Ivanir, Ester Segal
Summary: We present a label-free optical biosensor based on porous silicon (PSi) for sensitive and continuous detection of protein biomarkers in gastrointestinal fluids. The biosensor can continuously monitor the target protein in complex GI fluids without sample preparation and washing steps.
Article
Biophysics
Na Liu, Xueping Xiang, Lei Fu, Qiang Cao, Rong Huang, Huan Liu, Gang Han, Lidong Wu
Summary: Researchers have developed a dopamine biosensor using gold-coated magnetic nanoparticles for monitoring and regeneration, showing high sensitivity and stability. The sensor has been successfully applied in dopamine analysis in fish brains, paving the way for reusable and cost-effective production.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Oncology
Gaspar P. Pinto, Natalie M. Hendrikse, Jan Stourac, Jiri Damborsky, David Bednar
Summary: This article discusses the use of virtual screening tools to accelerate the development of anticancer drugs based on microbial products. It provides a detailed overview of the principles, workflow, and practical applications, showing the potential to expedite the discovery of novel cancer drugs.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Michal Vasina, Jan Velecky, Joan Planas-Iglesias, Sergio M. Marques, Jana Skarupova, Jiri Damborsky, David Bednar, Stanislav Mazurenko, Zbynek Prokop
Summary: Therapeutic enzymes are valuable biopharmaceuticals that have found successful applications in various biomedical fields. The integration of computational approaches and high-throughput experimental technologies has greatly accelerated the identification and engineering of catalytic therapeutic agents, leading to the discovery of new enzymes and the improvement of their properties.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Chemistry, Physical
Lea R. Rapp, Sergio M. Marques, Bernd Nebel, Jiri Damborsky, Bernhard Hauer
Summary: The catalytic space of the P450 monooxygenase CYP153A(M.aq) was transformed from a fatty acid hydroxylase to a catalyst capable of omega-hydroxylation of dodecylamine. A rapid screening method called RAPP was established to measure saturation libraries directly from a 96-deepwell plate. Molecular modeling and dynamics simulations revealed significant effects of the mutations on the substrate tunnel architectures.
Article
Biochemistry & Molecular Biology
Mihaly Varadi, Stephen Anyango, David Armstrong, John Berrisford, Preeti Choudhary, Mandar Deshpande, Nurul Nadzirin, Sreenath S. Nair, Lukas Pravda, Ahsan Tanweer, Bissan Al-Lazikani, Claudia Andreini, Geoffrey J. Barton, David Bednar, Karel Berka, Tom Blundell, Kelly P. Brock, Jose Maria Carazo, Jiri Damborsky, Alessia David, Sucharita Dey, Roland Dunbrack, Juan Fernandez Recio, Franca Fraternali, Toby Gibson, Manuela Helmer-Citterich, David Hoksza, Thomas Hopf, David Jakubec, Natarajan Kannan, Radoslav Krivak, Manjeet Kumar, Emmanuel D. Levy, Nir London, Jose Ramon Macias, Madhusudhan M. Srivatsan, Debora S. Marks, Lennart Martens, Stuart A. McGowan, Jake E. McGreig, Vivek Modi, R. Gonzalo Parra, Gerardo Pepe, Damiano Piovesan, Jaime Prilusky, Valeria Putignano, Leandro G. Radusky, Pathmanaban Ramasamy, Atilio O. Rausch, Nathalie Reuter, Luis A. Rodriguez, Nathan J. Rollins, Antonio Rosato, Luis Serrano, Gulzar Singh, Petr Skoda, Carlos Oscar S. Sorzano, Jan Stourac, Joanna Sulkowska, Radka Svobodova, Natalia Tichshenko, Silvio C. E. Tosatto, Wim Vranken, Mark N. Wass, Dandan Xue, Daniel Zaidman, Janet Thornton, Michael Sternberg, Christine Orengo, Sameer Velankar
Summary: PDBe-KB is an open collaboration platform that aims to integrate functional and biophysical annotations from world-leading specialist data resources, serving the Protein Data Bank. By developing standardized data exchange formats and integrating functional annotations from partner resources, PDBe-KB aims to place macromolecular structure data in a biological context and provide valuable biological insights.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Joan Planas-Iglesias, Filip Opaleny, Pavol Ulbrich, Jan Stourac, Zainab Sanusi, Gaspar P. Pinto, Andrea Schenkmayerova, Jan Byska, Jiri Damborsky, Barbora Kozlikova, David Bednar
Summary: The transplantation of loops between structurally related proteins is a method to improve enzyme activity, specificity, and stability. However, there are limited methods for loop-based rational protein design. The unique dynamism and identification of successful excision points are practical challenges. LoopGrafter is a web server that guides users through the loop grafting process, providing step-by-step procedures for loop identification, calculation of geometry, assessment of similarities and dynamics, and selection of loops for transplantation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Antonin Kunka, David Lacko, Jan Stourac, Jiri Damborsky, Zbynek Prokop, Stanislav Mazurenko
Summary: The quantitative description of protein unfolding and aggregation is important for the design of stability and understanding molecular basis of protein misfolding diseases. CalFitter 2.0 incorporates singular value decomposition of multi-wavelength spectral datasets, allowing fitting of different experimental data types and providing spectral fingerprints during unfolding.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Physical
Isabel Pardo, David Bednar, Patricia Calero, Daniel C. Volke, Jiri Damborsky, Pablo I. Nikel
Summary: This study discovered a highly efficient nonconventional fluorinase and demonstrated its potential in increasing the production of fluoronucleotides in a bacterial host, paving the way for efficient biohalogenation.
Article
Chemistry, Physical
Ren Wei, Gerlis von Haugwitz, Lara Pfaff, Jan Mican, Christoffel P. S. Badenhorst, Weidong Liu, Gert Weber, Harry P. Austin, David Bednar, Jiri Damborsky, Uwe T. Bornscheuer
Summary: Polyethylene terephthalate (PET) is a widely used synthetic polyester in textile fibers and packaging materials, which contributes significantly to global solid waste and environmental plastic pollution. Enzymatic PET recycling and upcycling have emerged as potential solutions, but there are limitations such as unbalanced enzyme-substrate interactions, limited thermostability, low catalytic efficiency at high temperatures, and inhibition by degradation intermediates. Protein engineering has been successful in addressing these limitations and can be applied to other mass-produced polymer types for biotechnological waste disposal.
Article
Multidisciplinary Sciences
Anna C. Papageorgiou, Michaela Pospisilova, Jakub Cibulka, Raghib Ashraf, Christopher A. Waudby, Pavel Kaderavek, Volha Maroz, Karel Kubicek, Zbynek Prokop, Lumir Krejci, Konstantinos Tripsianes
Summary: In this study, the authors propose a three-step charge-driven coacervation model involving dynamic complexes between a positively charged IDR of human RECQ4 and G-quadruplexes. The IDR also interacts with Replication Protein A, implying RECQ4's regulatory role.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Petr Kouba, Pavel Kohout, Faraneh Haddadi, Anton Bushuiev, Raman Samusevich, Jiri Sedlar, Jiri Damborsky, Tomas Pluskal, Josef Sivic, Stanislav Mazurenko
Summary: Recent progress has been made in engineering biocatalysts using machine learning methods. These methods utilize experimental and simulation data to discover and annotate enzymes, as well as suggest beneficial mutations. The field is expanding to cover various aspects of protein engineering, but there are still challenges to overcome. This Perspective provides an overview of ongoing trends, highlights case studies, and discusses limitations of machine learning-based methods.
Article
Biochemical Research Methods
Ondrej Vavra, Jakub Beranek, Jan Stourac, Martin Surkovsky, Jiri Filipovic, Jiri Damborsky, Jan Martinovic, David Bednar
Summary: Access pathways are critical for substrate and product transport in enzymes. A Python3 API called pyCaverDock is introduced to enhance the user experience and facilitate ligand transport analysis. This API simplifies the steps needed to use CaverDock and allows for automation of setup processes.
Article
Chemistry, Physical
Jan Mican, Da 'san M. M. Jaradat, Weidong Liu, Gert Weber, Stanislav Mazurenko, Uwe T. Bornscheuer, Jiri Damborsky, Ren Wei, David Bednar
Summary: Enzymatic PET recycling is a promising alternative to conventional methods, but requires optimization of PET hydrolases through engineering. A workflow integrating various methods can accelerate the discovery of PET-degrading enzymes, leading to the development of innovative plastic recycling techniques.
APPLIED CATALYSIS B-ENVIRONMENT AND ENERGY
(2024)
Article
Biochemistry & Molecular Biology
Anna Spackova, Ondrej Vavra, Tomas Racek, Vaclav Bazgier, David Sehnal, Jiri Damborsky, Radka Svobodova, David Bednar, Karel Berka
Summary: ChannelsDB 2.0 is an updated database that provides structural information about the position, geometry, and physicochemical properties of protein channels and pores. It incorporates data calculated by CAVER and MOLE tools, as well as additional information from AlphaFill database, resulting in significantly expanded channel annotations. The database stores information such as geometrical features and physico-chemical properties based on channel-lining amino acids, and is interlinked with UniProt mutation annotation data. ChannelsDB 2.0 serves as an important resource for in-depth analysis of the role of biomacromolecular tunnels and pores.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Sergio M. Marques, Michaela Slanska, Klaudia Chmelova, Radka Chaloupkova, Martin Marek, Spencer Clark, Jiri Damborsky, Eric T. Kool, David Bodnar, Zbynek Prokop
Summary: HaloTag labeling technology has revolutionized protein chemistry and cellular biology, and a systematic analysis of different ligands has led to the discovery of an optimal protein tag selection protocol, eliminating the need for expensive and complex protein engineering.
Article
Biochemistry & Molecular Biology
Dmitri Nikitin, Jan Mican, Martin Toul, David Bednar, Michaela Peskova, Patricia Kittova, Sandra Thalerova, Jan Vitecek, Jiri Damborsky, Robert Mikulik, Sarel J. Fleishman, Zbynek Prokop, Martin Marek
Summary: Cardio- and cerebrovascular diseases are major causes of death and disability, but the discovery of bacterial and human plasminogen activators has revolutionized their treatment. This study redesigned the molecular surface of staphylokinase (SAK) computationally to increase its affinity for plasmin. The engineered SAK mutant showed significantly enhanced affinity, selectivity, and efficiency in generating plasmin, providing a framework for the development of next-generation thrombolytic drugs.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)