Article
Immunology
Mohsen Golabi, Marion Flodrops, Beatrice Grasland, Aaydha C. Vinayaka, Than Linh Quyen, Trieu Nguyen, Dang Duong Bang, Anders Wolff
Summary: The study analyzed a large number of gene sequences of newly isolated AIV within the EU and designed a highly efficient LAMP primer set that covers all AIV subtypes. The optimized real-time RT-LAMP assay demonstrated specificity and sensitivity comparable to the official standard rRT-PCR assay for detecting AIV samples belonging to nine various subtypes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Varsha Potdar, Megha Brijwal, Rakesh Lodha, Pragya Yadav, Santosh Jadhav, Manohar Lal Choudhary, Aashish Choudhary, Veena Vipat, Nivedita Gupta, Ashok Kumar Deorari, Lalit Dar, Priya Abraham
Summary: An 11-year-old boy with acute myeloid leukemia in New Delhi, India, was treated for severe acute respiratory infection caused by avian influenza A(H5N1). The strain belonged to hemagglutinin gene clade 2.3.2.1a and was found to be susceptible to amantadine and neuraminidase inhibitors.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Mariana Leguia, Alejandra Garcia-Glaessner, Breno Munoz-Saavedra, Diana Juarez, Patricia Barrera, Carlos Calvo-Mac, Javier Jara, Walter Silva, Karl Ploog, Lady Amaro, Paulo Colchao-Claux, Christine K. Johnson, Marcela M. Uhart, Martha I. Nelson, Jesus Lescano
Summary: Highly pathogenic avian influenza A/H5N1 viruses have invaded the Americas and pose a threat to wildlife, poultry, and humans. The HPAI/H5N1 viruses in Peru have genetic reassortment between Eurasian and American lineages, emphasizing the need for surveillance and control measures.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Wendy Puryear, Kaitlin Sawatzki, Nichola Hill, Alexa Foss, Jonathon J. Stone, Lynda Doughty, Dominique Walk, Katie Gilbert, Maureen Murray, Elena Cox, Priya Patel, Zak Mertz, Stephanie Ellis, Jennifer Taylor, Deborah Fauquier, Ainsley Smith, Robert A. DiGiovanni Jr, Adriana van de Guchte, Ana Silvia Gonzalez-Reiche, Zain Khalil, Harm van Bakel, Mia K. Torchetti, Kristina Lantz, Julianna B. Lenoch, Jonathan Runstadler
Summary: We report the spread of highly pathogenic avian influenza A(H5N1) in marine mammals in the northeastern United States, which is concurrent with the presence of H5N1 in wild birds in the same area. Our findings highlight the importance of monitoring both wild coastal birds and marine mammals in assessing the potential for an influenza A virus pandemic.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Ariful Islam, Tasnim Ara, Emama Amin, Shariful Islam, Md. Abu Sayeed, Tahmina Shirin, Mohammad Mahmudul Hassan, Marcel Klaassen, Jonathan H. Epstein
Summary: Understanding the characteristics, dynamics, and evolution of H5N1 outbreaks in Bangladesh is crucial for the prevention and control of High Pathogenicity Avian Influenza (HPAI). Our study revealed nine waves of HPAI H5N1 outbreaks between 2007 and 2020, with peak incidences during the winter season. Despite vaccination efforts, the virus continued to circulate in Bangladesh, with significant clustering and hotspots of H5N1 outbreaks in and around Dhaka district. Four H5N1 clades have been detected, with clade 2.3.2.1a persisting since 2011 and reassortment occurring in 2012. Our findings emphasize the importance of spatiotemporal analysis and phylodynamics in developing targeted control measures.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Multidisciplinary Sciences
Natalie Moyen, Md. Ahasanul Hoque, Rashed Mahmud, Mahmudul Hasan, Sudipta Sarkar, Paritosh Kumar Biswas, Hossain Mehedi, Joerg Henning, Punam Mangtani, Meerjady Sabrina Flora, Mahmudur Rahman, Nitish C. Debnath, Mohammad Giasuddin, Tony Barnett, Dirk U. Pfeiffer, Guillaume Fournie
Summary: Live animal markets are known hotspots for zoonotic disease emergence, and trading networks play a crucial role in disease spread dynamics. In Bangladesh, live poultry trading networks promote viral strain co-circulation, with upstream transmission nodes significantly impacting viral prevalence in markets. Disease control interventions should target network structures, tailored to local characteristics for effectiveness.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Olivier Duriez, Yohan Sassi, Chloe Le Gall-Ladeveze, Lea Giraud, Robert Straughan, Lise Dauverne, Anna Terras, Thierry Boulinier, Remi Choquet, Anne van de Wiele, Julien Hirschinger, Jean-Luc Guerin, Guillaume Le Loc'h
Summary: A study found that terrestrial birds infected with highly pathogenic avian influenza (HPAI) virus exhibit immobility behavior during the breeding season, which may reduce the risk of virus transmission. However, these infections pose a threat to endangered vulture species, raising concerns about their resistance to the virus.
Article
Veterinary Sciences
Jiao Hu, Peipei Peng, Jun Li, Qi Zhang, Rumeng Li, Xiaoquan Wang, Min Gu, Zenglei Hu, Shunlin Hu, Xiaowen Liu, Xinan Jiao, Daxin Peng, Xiufan Liu
Summary: The bivalent H5+H7 VLP vaccine candidate shows promising results in protecting poultry against highly pathogenic H5N1 and H7N9 avian influenza viruses, and may serve as a critical alternative to traditional egg-based inactivated vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
V Caliendo, N. S. Lewis, A. Pohlmann, S. R. Baillie, A. C. Banyard, M. Beer, I. H. Brown, R. A. M. Fouchier, R. D. E. Hansen, T. K. Lameris, A. S. Lang, S. Laurendeau, O. Lung, G. Robertson, H. van der Jeugd, T. N. Alkie, K. Thorup, M. L. van Toor, J. Waldenstrom, C. Yason, T. Kuiken, Y. Berhane
Summary: Recent detection of highly pathogenic avian influenza viruses in North America, closely related to the viruses in Europe, raises concerns about further spread across the Americas through wild bird migration.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Nichola J. Hill, Mary Anne Bishop, Nidia S. Trovao, Katherine M. Ineson, Anne L. Schaefer, Wendy B. Puryear, Katherine Zhou, Alexa D. Foss, Daniel E. Clark, Kenneth G. MacKenzie, Jonathon D. Gass, Laura K. Borkenhagen, Jeffrey S. Hall, Jonathan A. Runstadler
Summary: The study provides insights into how a variety of avian hosts contribute to viral spread and spillover, potentially enhancing surveillance in a rapidly changing global environment.
Article
Biochemical Research Methods
Keine Nishiyama, Yohei Takeda, Kazuki Takahashi, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Haruko Ogawa, Manabu Tokeshi
Summary: Researchers developed a non-competitive fluorescence polarization immunoassay (NC-FPIA) for detecting H5 subtype avian influenza virus, achieving successful detection within 15 minutes and enabling rapid on-site diagnosis of various viruses.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Virology
Xu Zhou, Siwen Wang, Yue Ma, Yanbing Li, Guohua Deng, Jianzhong Shi, Xiurong Wang
Summary: A reverse transcription recombinase polymerase amplification (RT-RPA)/CRISPR method was developed for rapid and sensitive detection of avian influenza virus (AIV). The new system has the potential to be an accurate, user-friendly, and inexpensive platform for point-of-care testing applications.
Article
Fisheries
Yeojin Hong, Anh Duc Truong, Thi Hao Vu, Sooyeon Lee, Jubi Heo, Suyeon Kang, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study demonstrates the immunomodulatory effects of exosomes from HPAIV H5N1-infected chickens on immune-related cells, showing that these exosomes induce pro-inflammatory cytokine expression and stimulate the MAPK signaling pathway. Moreover, viral proteins from H5N1 are packaged in exosomes and transferred to non-infected immune-related cells.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Immunology
Sarah N. Bevins, Susan A. Shriner, James C. Cumbee, Krista E. Dilione, Kelly E. Douglass, Jeremy W. Ellis, Mary Lea Killian, Mia K. Torchetti, Julianna B. Lenoch
Summary: Eurasian-origin highly pathogenic avian influenza A(H5N1) virus belonging to the Gs/GD lineage, clade 2.3.4.4b, was detected in wild waterfowl in 2 Atlantic coastal states in the United States. Bird banding data revealed widespread movement of waterfowl within the Atlantic Flyway and between neighboring flyways and northern breeding grounds.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Leo Loth, Long Thanh Pham, Mark Anthony Stevenson
Summary: The study revealed statistically significant spatio-temporal clustering of HPAI-H5N1 outbreaks in poultry in Vietnam, with frequent outbreaks in the southern region. Shortcomings in control measures led to difficulties in effectively controlling the outbreaks, highlighting the need for a better understanding of the relative impact of various control measures on spatio-temporal interactions in order to focus efforts on the most effective measures.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Chemistry, Analytical
Meng-Meng Pan, Dongliang Feng, Yu Ouyang, Dachi Yang, Xu Yu, Li Xu, Itamar Willner
Summary: The study demonstrates that gas sensors constructed using Co3O4/ZnO SPs have high sensitivity and selectivity for rapid detection of foodborne pathogens. By discussing the gas sensing mechanism, a method for quickly detecting microbial contamination is proposed.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Meng-Meng Pan, Yu Ouyang, Yong-Li Song, Lu-Qin Si, Ming Jiang, Xu Yu, Li Xu, Itamar Willner
Summary: In this study, the synthesis and characterization of Au3+-modified UiO-67 metal-organic framework nanoparticles were described. The Au3+-NMOFs showed dual oxidase-like and peroxidase-like activities, and acted as an effective catalyst for the generation of ROS agents for antibacterial and wound healing applications.
Article
Chemistry, Analytical
Ya-qian Wang, Qin-ying Li, Ming Jiang, Xin Yang, XiaoWei Wu, Xu Yu, Li Xu
Summary: Water content is an important parameter that needs to be monitored for safety considerations in various fields. In this study, the natural isoquinoline alkaloid palmatine was used as a fluorescent probe to accurately determine water content in different organic solvents. Additionally, a novel fluorescent colorimetric probe based on the combination of palmatine and nitrogen doped carbon dots (N-CDs) was developed, allowing for the visual semi-quantification of water content. The use of palmatine as a fluorescent probe or in the palmatine/N-CDs probe showed promising results for the fast detection of water.
MICROCHEMICAL JOURNAL
(2022)
Article
Environmental Sciences
Yi-Fan Wang, Zhi Li, Ming Jiang, Xu Yu, Li Xu
Summary: In this study, two-in-one silicon nanoparticles were designed and prepared as a fluorescent probe and degradation catalyst for the detection and removal of tetracycline antibiotics. These nanoparticles can selectively quench the blue fluorescence upon interaction with tetracycline, while emitting yellow-green fluorescence. They can be used as fluorescent probes for the detection of doxycycline, oxytetracycline, and tetracycline with low limits of detection. Additionally, the nanoparticles were capable of catalyzing the degradation of the antibiotics under UV light. This study provides a new strategy for the rapid and simple detection and removal of drug pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Physical
Zhi Li, Xu Yu, Ming Jiang, Li Xu
Summary: In this study, silicon-doped polymer dots (Si-PEIPDs) were synthesized, which exhibited stable blue fluorescence and effective antibacterial properties against E. coli and S. aureus. The Si-PEIPDs also possessed free radicals scavenging ability, which could facilitate the healing of bacterial infected wounds. Furthermore, Si-PEIPDs acted as capping and reduction agents for the synthesis of gold nanoparticles, forming nanocomposites with higher catalysis activity. This research opens up a new avenue for the multiple applications of polymer dots in environmental remediation and the medical field.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Analytical
Song Huang, Le Wang, Ming Wang, Jing Zhao, Cong Zhang, Li-Yun Ma, Ming Jiang, Li Xu, Xu Yu
Summary: Researchers have developed a ZnO nanorods integrated microarray chip (ZnO-chip) for effective capture and sensitive detection of extracellular vesicles (EVs), which have potential as biomarkers for cancer diagnosis in liquid biopsy. The chip provides abundant binding sites for EVs capture and utilizes cascade signal amplification for accurate quantitation. The method has the ability to distinguish cancer patients from healthy individuals and could be applied for routine screening in clinical trials.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yi-Fan Wang, Xu Yu, Zhi Li, Meng-meng Pan, Ming Jiang, Li Xu
Summary: In this study, N doped silicon quantum dots (N-SiQDs) with adjustable fluorescence colors were synthesized by controlling preparation conditions. The N-SiQDs were used as a fluorescent probe for the detection of Cu2+ in tap water and artificial cerebrospinal fluid, as well as for cell imaging and intracellular detection of Cu2+. The study also demonstrated the potential of N-SiQDs for optical device applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ya-Qian Wang, Li Li, Jin Yin, Xu Yu, Xiaowei Wu, Li Xu
Summary: In this study, silane reagents were explored as fluorescence sensitizers for the first time. It was found that 3-glycidoxypropyltrimethoxysilane (GPTMS) had the strongest sensitization effect. GPTMS was adopted as a novel fluorescence sensitizer to significantly enhance the fluorescence of curcumin for detection. The method showed high accuracy in determining curcumin in various food samples and had potential applications in solid fluorescence systems.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Environmental
Xiao Lei, Li-Jun Ma, Si-Nan Yu, Tian-Ling Ren, Shi-Feng Li, Jun Yuan, Lei Ye, Xu Yu, Feng Liu
Summary: Inspired by biological skin, a smart skin with a skin-like microchannel (SMC) structure is developed using 3D printing, which can simultaneously realize the sensing function of pressure and the actuating function of fluid. The smart skin has an ultrahigh sensitivity of 1363.9 kPa-1 in the pressure range of 0-5 kPa, surpassing existing e-skin. Additionally, it can achieve diversified intelligent functions by introducing various functional fluids into the SMC structure, such as drug delivery, self-backup of sensing signal, and visual overload alarm.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Li Li, Wan-Chun Luo, Ming Jiang, Xu Yu, Li Xu
Summary: The aggregation of amyloid proteins is closely related to neurodegenerative and metabolic diseases. Detecting amyloid fibrils is essential for understanding the diseases and advancing drug discovery and treatment. In this study, three proto-berberine alkaloids were examined as fluorescent probes for detecting amyloid fibrils. These alkaloids exhibited sensitivity to viscosity and polarity, enabling them to probe insulin and lysozyme fibrils with turn-on fluorescence without interfering with the fibrillation process. They also successfully monitored the aggregation process of amyloid proteins in vitro and imaged the fibrils in living cells, showing their potential as candidate fluorescent probes for amyloid fibrils.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Qin-ying Li, Xu Yu, Xi Li, Li-na Bao, Yu Zhang, Shi-lin Wang, Ming Jiang, Kun Huang, Li Xu
Summary: In this study, three carbon dots (CDs) derived from Congo red were successfully synthesized and demonstrated the potential for diagnosis and treatment of protein aggregation diseases. Among them, citric acid-derived CDs showed the most outstanding performance, especially in cytoprotection against Alzheimer's disease-related proteins. This study opens a new pathway for protein disease research.
Article
Chemistry, Analytical
Yong-Li Song, Xiang-Lan He, Yong Li, Ming Wang, Ming Jiang, Li Xu, Xu Yu
Summary: In this study, a novel method called SRPL-TraCs is introduced, which combines selective recognition proximity ligation and signal amplification strategies to construct a high-order cascade signal amplification strategy for detecting biomarkers. This method shows excellent specificity and potential, not only for nucleic acids, but also for other interesting targets.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Mengmeng Pan, Ming Wang, Linjiao Yang, Yongli Song, Ming Jiang, Xu Yu, Li Xu
Summary: In this study, a novel nanozyme was synthesized for rapid and highly sensitive detection of alkaline phosphatase (ALP). The biosensor constructed with this nanozyme showed excellent detection performance in both buffer and clinical samples. Additionally, a smartphone-based visual detection method was developed, enabling portable testing in resource-limited regions.
Article
Engineering, Environmental
Yan-Ping Yu, Meng -Meng Pan, Ming Jiang, Xu Yu, Li Xu
Summary: A three-dimensional flower-like Cu-MOF was successfully synthesized and its pyrolytic derivative Cu-N-C450 was obtained. The Cu-MOF exhibited laccase-like activity and excellent antibacterial ability to Escherichia coli. Additionally, the Cu-MOF served as an adsorbent to remove environmental pollutants and the Cu-N-C450 efficiently catalyzed the degradation of methylene blue.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Yi-Fan Wang, Meng-Meng Pan, Yong-Li Song, Zhi Li, Le Wang, Ming Jiang, Xu Yu, Li Xu
Summary: This study successfully synthesized a versatile nitrogen-doped silicon quantum dot (N-SiQD) and demonstrated its potential applications in bacterial imaging, synthesis of nanocomposites, and antimicrobial properties.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
