期刊
BIOSENSORS & BIOELECTRONICS
卷 82, 期 -, 页码 212-216出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.04.014
关键词
Electrochemical sensor; Hepatitis B virus gene sequence; Point mutation discrimination; Methylene blue
类别
资金
- Returned Overseas Scholars Fund in Heilongjiang Province [LC2013C04]
- National Natural Science Foundation of China [51473042, 21371041]
- Natural Scientific Fund of Heilongjiang Province of China [C201301]
- Science and Technology Key Projects in Heilongjiang Province Department of Education [12541z005]
One signal on electrochemical sensing strategy was constructed for the detection of a specific hepatitis B virus (HBV) gene sequence based on the protection-displacement-hybridization-based (PDHB) signaling mechanism. This sensing system is composed of three probes, one capturing probe (CP) and one assistant probe (AP) which are co-immobilized on the Au electrode surface, and one 3-methylene blue (MB) modified signaling probe (SP) free in the detection solution. One duplex are formed between AP and SP with the target, a specific HBV gene sequence, hybridizing with CP. This structure can drive the MB labels close to the electrode surface, thereby producing a large detection current. Two electrochemical testing techniques, alternating current voltammetry (ACV) and cyclic voltammetry (CV), were used for characterizing the sensor. Under the optimized conditions, the proposed sensor exhibits a high sensitivity with the detection limit of similar to 5 fM for the target. When used for the discrimination of point mutation, the sensor also features an outstanding ability and its peculiar high adjustability. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据