期刊
BIOSENSORS & BIOELECTRONICS
卷 82, 期 -, 页码 26-31出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.03.057
关键词
Near-infrared; Electrochemiluminescence resonance energy transfer; CdTe/CdS quantum dots; Gold nanorods; Thrombin; Aptasensor
类别
资金
- National Natural Science Foundation of China [21375043, 21405139]
Here we designed a near-infrared electrochemiluminescence (NECL) aptasensor for turn-on ultra sensitive determination of thrombin. It was based on the ECL resonance energy transfer (ECL-RET) of CdTe/CdS core(small)/shell(thick) quantum dots (QDs) to gold nanorods (AuNRs). AuNRs which functioned as ECL acceptors were assembled onto CdTe/CdS film by DNA hybridization between aptamers and their complementary oligonucleotides. In the absence of thrombin, the NECL of QDs was quenched as a result of the ECL-RET of QDs to AuNRs. In the presence of thrombin, the NECL of the system was turned on because thrombin can replace the AuNRs onto the QDs film, owing to the specific aptamer-protein affinity interactions. In this way, the increment of ECL intensity and the concentration of thrombin showed a logarithmic linear correlation in the range of 100 aM to 10 fM with a detection limit of 31 aM (S/N = 3). Importantly, the developed aptasensor was successfully applied to thrombin sensing in real serum samples. (C) 2016 Elsevier B.V. All rights reserved.
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