Article
Biotechnology & Applied Microbiology
Yuhuan Huang, Mao Chen, Guoquan Hu, Bo Wu, Mingxiong He
Summary: In this study, a theophylline-dependent riboswitch was utilized to regulate the expression of editing plasmids in Zymomonas mobilis, resulting in increased plasmid elimination efficiency and successful genome editing.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Mao Chen, Yuhuan Huang, Yudi Zheng, Bo Wu, Mingxiong He
Summary: This study reveals the presence of gene escape in Zymomonas mobilis yeast after gene knockout using CRISPR system. Different outcomes of gene-editing events were observed, with some genes easily knocked out and the editing plasmid successfully cured, while in other cases, the editing plasmid was difficult to remove or the deleted genes were transferred into the plasmid. Bacterial insertion sequences may contribute to gene transfer. Inadequate validation in other microbes may lead to underestimation of escape events.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Biochemical Research Methods
Shujie Gu, Jie Zhang, Lili Li, Jin Zhong
Summary: This study established the first endogenous CRISPR-Cas9 genome-editing system in lactobacilli, achieving efficient gene editing, deletion of large genomic fragments, and simultaneous deletion of multiple genes. A controllable plasmid-targeting sgRNA expression module was designed for high-efficiency gene deletion and plasmid curing.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Microbiology
Xiaojing Wang, Yufei Lyu, Siya Wang, Qingfang Zheng, Erling Feng, Li Zhu, Chao Pan, Shenghou Wang, Dongshu Wang, Xiankai Liu, Hengliang Wang
Summary: The CRISPR-Cas system has been effectively applied in prokaryotic genome editing. By utilizing scissors plasmids, researchers were able to successfully eliminate native plasmids from Bacillus anthracis and Bacillus cereus, as well as specifically kill B. anthracis. The efficiency of plasmid curing and sterilization of B. anthracis was high, providing a promising method to study these bacteria and their virulence profiles.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Matthew S. H. Lau, Lili Sheng, Ying Zhang, Nigel P. Minton
Summary: This study expands on the genetic tools available for engineering the industrial chassis Parageobacillus thermoglucosidasius by assembling and testing a range of promoters, ribosome binding sites, reporter genes, and implementing CRISPR/Cas9 genome editing based on two different thermostable Cas9 nucleases.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jiao Li, Xin Song, Zhiqiang Xiong, Guangqiang Wang, Yongjun Xia, Yijin Yang, Lianzhong Ai
Summary: In this study, the CRISPR system of B. animalis AR668 was successfully established, and genes 0348 and 0208 were effectively knocked out. The impact of different homology arms and fragments on the knockout efficiency of the system was explored. Additionally, an innovative inducible plasmid curing system for bifidobacteria was established. This study contributes significantly to the genetic modification and functional mechanism analysis of bifidobacteria.
MICROBIAL CELL FACTORIES
(2023)
Article
Nanoscience & Nanotechnology
Akbar Hasanzadeh, Amir Hossein Vahabi, Seyyed Emad Hooshmand, Elaheh Sadat Hosseini, Behjat Kheiri Yeghaneh Azar, Jafar Kiani, Sara Saeedi, Ali Shahbazi, Arnab Rudra, Michael R. Hamblin, Mahdi Karimi
Summary: In this study, a highly efficient gene carrier, PEI-PBA-SAP-F, was developed. It showed outstanding transfection efficiency for both small plasmid DNA and large plasmid DNA into various cells, even at low plasmid doses and high serum concentrations. Additionally, it successfully delivered different types of CRISPR/Cas9 complexes.
Article
Microbiology
Megha Sodani, Chitra S. Misra, Devashish Rath, Savita Kulkarni
Summary: The rapid emergence of drug resistance has created challenges for the treatment of mycobacterial infections. Alternative treatment approaches, such as CRISPR-Cas systems, are needed due to the slow development of new drugs. This study demonstrates the antimicrobial potential of CRISPR-systems against drug-resistant mycobacterial infections and provides evidence for the sequence-specific targeting of antibiotic-resistant factors.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bo-Chou Chen, Yu-Zhen Chen, Huan-Yu Lin
Summary: The CRISPR-Cas system is widely used for genome editing due to its convenience, simplicity, and flexibility. This article describes a plasmid-curing strategy based on the CRISPR-Cpf1 system and proposes a simplified plasmid-curing system. The method efficiently removes the plasmid and allows for iterative processes in multiple rounds of genome editing.
Article
Biochemistry & Molecular Biology
Xiaojie Wang, Bo Wu, Xin Sui, Zhufeng Zhang, Tao Liu, Yingjun Li, Guoquan Hu, Mingxiong He, Nan Peng
Summary: CRISPR-Cas systems provide adaptive immunity to bacteria and archaea by absorbing spacers, but self-derived spacers can cause CRISPR self-interference; the species efficiently repairs CRISPR-Cas system-mediated DNA breaks using the MMEJ pathway.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Article
Plant Sciences
Minjeong Kang, Keunsub Lee, Todd Finley, Hal Chappell, Veena Veena, Kan Wang
Summary: This paper presents an advanced B104 transformation protocol that can generate transgenic plants in a quicker and simplified manner, suitable for genome editing applications. The success rate of this protocol is high, making it a valuable tool for researchers in the field.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Xin Sui, Xiaojie Wang, Tao Liu, Qing Ye, Bo Wu, Guoquan Hu, Shihui Yang, Mingxiong He, Nan Peng
Summary: A novel gene deletion tool based on the endogenous subtype I-F CRISPR-Cas system and the MMEJ pathway was developed for Zymomonas mobilis, enabling the efficient deletion of single genes and large fragments. Modifications to the guide RNA expression cassette and target site selection further improved editing efficiency.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Juan Pablo Gomez-Escribano, Neil A. Holmes, Susan Schlimpert, Maureen J. Bibb, Govind Chandra, Barrie Wilkinson, Mark J. Buttner, Mervyn J. Bibb
Summary: Streptomyces venezuelae has been widely used as a model strain for studying molecular mechanisms controlling morphological development in streptomycetes. Its rapid growth and ability to sporulate in a near-synchronized manner in liquid culture make it suitable for modern molecular techniques. A high-quality genome sequence of the strain was described, revealing differences in spore pigmentation and the successful use of CRISPR-Cas9 to cure the plasmid without noticeable phenotypic consequences.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mimi Hu, Weiwei Bao, Qiqun Peng, Wei Hu, Xinyu Yang, Yan Xiang, Xiongying Yan, Mian Li, Ping Xu, Qiaoning He, Shihui Yang
Summary: In this study, a lactate producer of Z. mobilis was constructed by metabolic engineering and fermentation optimization. The engineered strain showed a high lactate production efficiency and a high total carbon conversion rate. It could efficiently produce lactate using waste feedstocks. This study demonstrated the potential of this lactate-producing strain as a biorefinery platform for carbon-neutral biochemical production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Agronomy
Yonghua Dai, Bo Wu, Panting Liu, Mao Chen, Chao Song, Qiyu Gou, Renbin Liu, Yansheng Xu, Guoquan Hu, Mingxiong He
Summary: A low-cost process for ethanol production from sugarcane was developed using genetically engineered Zymomonas mobilis, resulting in increased efficiency and productivity of ethanol fermentation. Fed-batch fermentation using optimized media and engineered strain achieved high ethanol titer and productivity, demonstrating the feasibility of economic ethanol production in the future.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Psychology, Clinical
Xiaojing Li, Wei Deng, Rui Xue, Qiang Wang, Hongyan Ren, Wei Wei, Yamin Zhang, Mingli Li, Liansheng Zhao, Xiangdong Du, Yajing Meng, Xiaohong Ma, Mei-Hua Hall, Tao Li
Summary: This study found deficits in event-related potential, including duration mismatch negativity and P3a, in drug naive patients with chronic schizophrenia and bipolar disorder. These deficits appeared to be related to levels of higher-order cognition and functioning.
PSYCHOLOGICAL MEDICINE
(2023)
Article
Materials Science, Ceramics
Renzhong Xue, Liuyang Zhao, Siyuan Chen, Hongxuan Chen, Mengru Cui, Xintao Bai, Tao Li, Dewei Liu, Haiyang Dai
Summary: The effects of A-site transition from Ca2+ to Cd2+ on the microstructure, morphology, and electrical properties of Ca(1-x)CdxCu(3)Ti(4)O(12) thin films were investigated. The films exhibited smooth and compact surfaces without cracks. The transition from Ca2+ to Cd2+ resulted in changes in grain size and electrical properties. In particular, the simultaneous occupancy of A sites by Ca2+ and Cd2+ improved the dielectric and nonlinear properties of Ca1-xCdxCu3Ti4O12 films.
CERAMICS INTERNATIONAL
(2023)
Article
Plant Sciences
Ying Zhang, Chunli Guo, Hongmei Liu, Lin Yang, Changying Ren, Tao Li, Jianxun Liu
Summary: This study established a highly sensitive and accurate quantitative method to reveal the plasma exposure profile of Sailuotong (SLT) in humans. The developed methods should be useful in further detailed clinical pharmacokinetic research for clarifying the effect mechanism of SLT and formulating its rational therapeutic regimens.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Engineering, Industrial
Xiaoli Yan, Tao Li
Summary: This paper aims to analyze the role and effect of Hangzhou Urban Brain in responding to COVID-19, including systematically sorting out its development mode, capabilities, composition and application, and exploring its role and effect. It tries to provide a reference for other cities' digital infrastructure construction through case analysis.
ENGINEERING CONSTRUCTION AND ARCHITECTURAL MANAGEMENT
(2023)
Article
Computer Science, Artificial Intelligence
Meng Yuan, Jiucheng Xu, Tao Li, Yuanhao Sun
Summary: This paper introduces a feature selection method based on neighborhood multi-granulation rough sets (NMRS) to address the information loss issue. A novel measure, named PTSIJE, is proposed for uncertain feature selection in incomplete datasets. Experimental results demonstrate the effectiveness of the proposed method.
COMPLEX & INTELLIGENT SYSTEMS
(2023)
Article
Materials Science, Ceramics
Haiyang Dai, Qinlong Shen, Jing Chen, Zijiong Li, Zhenping Chen, Luogang Xie, Tao Li
Summary: Cu1_xHoxFeO2 nanoceramics were synthesized by sol-gel method to investigate the influence of vacancy defects and Fe valence state tuning on their magnetic and dielectric properties. XRD and Raman analysis confirmed the formation of single-phase delafossite structure. XPS analysis revealed that Ho3+ substitution could tune the Fe valence state, while positron annihilation lifetime spectroscopy showed that it could effectively tailor the cation vacancies concentration in the interface region. Magnetic measurements indicated that Ho3+ substitution enhanced the magnetic transition temperatures and magnetization, while Cu1_xHoxFeO2 nanoceramics exhibited giant dielectric characteristic attributed to charge ordering of Fe3+ and Fe2+.
CERAMICS INTERNATIONAL
(2023)
Article
Clinical Neurology
Zhili Zou, Yuan Zhang, Yulan Huang, Jinyu Wang, Wenjiao Min, Miao Xiang, Bo Zhou, Tao Li
Summary: Differential DNA methylation and mRNA expression were investigated between panic disorder (PD) and healthy controls (HCs). Changes in expression level of the CBL gene may serve as an important molecular marker for diagnosis and early response in PD patients.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Electrochemistry
Dewei Liu, Qijie Zhang, Xiaohong Chen, Hui Li, Fufeng Yan, Haiyang Dai, Tao Li, Renzhong Xue, Jing Chen, Gaoshang Gong, Cui Shang, Xuezhen Zhai
Summary: The effects of Mg doping on the structure, morphology, defects, and electrochemical performances of Mg-doped VO2(B) nanorods were investigated. The results showed that moderate Mg doping improved the structural stability and cycling performance of the samples.
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Bing-jun Chen, Jian Jiang, Tan Li, Hong-jie Jiang, Xin-hua Liang, Ya-ling Tang
Summary: The invasion and metastasis of salivary adenoid cystic carcinoma (SACC) is closely related to collective invasion. The downregulation of FAT1 and upregulation of YAP1 are associated with collective invasion and distant metastasis in SACC. The miR-183-5p/FAT1/YAP1 signaling pathway plays a critical role in SACC collective invasion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Kai Guo, Xue Ren, Ting-Ting Zhou, Tao Li, Yan-Chun Liu, Yang Tao, Hong Hu, Dan Li, Yan Liu, Sheng-Hong Li
Summary: Systematic phytochemical investigation of Lomatogonium carinthiacum led to the identification of 12 monoterpenoids, including three new secoiridoids (1, 2, and 4), a new iridoid glycoside (13), a new monoterpenoid alkaloid (3), and three new sesquiterpenoids (14-16). The structures were determined through comprehensive spectroscopic analysis and quantum chemistry computations. The new isolates exhibited weak immunosuppressive activities by inhibiting T cell proliferation and cytokine IFN-gamma secretion in vitro.
Article
Chemistry, Multidisciplinary
Jitao Ji, Zhizhang Wang, Jiacheng Sun, Chen Chen, Xueyun Li, Bin Fang, Shi-Ning Zhu, Tao Li
Summary: In this study, we propose a novel method for on-chip generation and manipulation of higher-order Poincare sphere beams (HOPBs) by combining metasurface with optical waveguides on a lithium niobate on insulator platform. By using a diatomic geometric metasurface for phase modulation, guided waves are extracted into free space with high signal-to-noise ratio and formed into two orthogonal circularly polarized optical vortices which are linearly superposed into HOPBs. Additionally, a dual-port waveguide crossing is utilized to reconfigure the output states into an arbitrary point on a higher-order Poincare sphere based on in-plane interference of two guided waves. This approach provides a compact solution for generating and manipulating HOPBs and can be further enhanced by employing electro-optical modulation on a lithium niobate waveguide for full tunability.
Article
Optics
Yanhao Chu, Xingjian Xiao, Xin Ye, Chen Chen, Shining Zhu, Tao Li
Summary: In this study, a design method is proposed to achieve large scale achromatic hybrid lens with no residual dispersion by considering the refraction element and metasurface together as a whole. The tradeoff between the meta-unit library and the characteristics of resulting hybrid lenses is also discussed in detail, and a centimeter scale achromatic hybrid lens is realized to demonstrate the effectiveness of the method.
Article
Polymer Science
Lin Xu, Samuel C. Lamont, Tao Li, Ying Zhang, Wenlong Pan, Chongyi Gao, Chen Zhu, Si Chen, Hongwei Hu, Jianning Ding, Franck J. Vernerey
Summary: We investigated the mechanical properties of a magnetic temperature-sensitive hydrogel with varying concentrations of covalent and physical cross-linking. Increasing the nanoclay content improved both strength and toughness in the double network materials due to the strong viscoplastic behavior of the physical nanoclay network. Our findings suggest that the yield-stress behavior of the nanoclay network and load-sharing interactions between the PNIPAAm and nanoclay are responsible for the observed trends. Additionally, we found a strong correlation between the mass ratio of covalent cross-linker used and fractional percolation of the PNIPAAm network.
Article
Engineering, Biomedical
Xi Li, Xiangyi Ren, Maodi Xie, Mengli Zhu, Yabing Zhang, Tao Li, Minfeng Huo, Qian Li
Summary: Researchers have synthesized BSA-coated RuO2 nanoparticles that can scavenge reactive oxygen species and reduce myocardial ischemia/reperfusion injury. These nanoparticles mimic enzymes and can reduce infarcted areas and restore cardiac function. They have high clinical potential for IR injury treatment.
ADVANCED NANOBIOMED RESEARCH
(2023)
Article
Optics
Shanshan Hu, Xingjian Xiao, Xin Ye, Rongtao Yu, Yanhao Chu, Ji Chen, Shining Zhu, Tao Li
Summary: Correction of chromatic aberration is crucial in color imaging and display. This study proposes a deep-learning-enhanced singlet planar imaging system using an achromatic flat lens, achieving high-quality achromatic imaging in the visible spectrum.