期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 80, 期 10, 页码 1907-1916出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2016.1191328
关键词
drought avoidance; Ran GTPase; root growth; arid region; wild watermelon
类别
资金
- Asia and Africa Science Platform Program
- Japan Society for the Promotion of Science
- Ministry of Education, Science, Sports and Culture
- SATREPS, ALCA
- Core Research for Evolutional Science and Technology
- CREST Programs of Japan Science and Technology Agency
- Nissan Science Foundation
- Foundation for Nara Institute of Science and Technology
- MRA Project of the Arid Land Research Center, Tottori University
Enhanced root growth is known as the survival strategy of plants under drought. Previous proteome analysis in drought-resistant wild watermelon has shown that Ran GTPase, an essential regulator of cell division and proliferation, was induced in the roots under drought. In this study, two cDNAs were isolated from wild watermelon, CLRan1 and CLRan2, which showed a high degree of structural similarity with those of other plant Ran GTPases. Quantitative RT-PCR and promoter-GUS assays suggested that CLRan1 was expressed mainly in the root apex and lateral root primordia, whereas CLRan2 was more broadly expressed in other part of the roots. Immunoblotting analysis confirmed that the abundance of CLRan proteins was elevated in the root apex region under drought stress. Transgenic Arabidopsis overexpressing CLRan1 showed enhanced primary root growth, and the growth was maintained under osmotic stress, indicating that CLRan1 functions as a positive factor for maintaining root growth under stress conditions.
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