期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 80, 期 6, 页码 1223-1229出版社
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2016.1141040
关键词
Euglena; rapamycin; biofuel; Chlamydomonas; Cyanidioschyzon
类别
资金
- Strategic International Cooperative Program (SICORP) Japanese (JST) - US (NSF) Joint Research Metabolomics for a low carbon society (METABOLOMICS)
- commission for Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI), Japan
Rapamycin induces autophagy with lipid remodeling in yeast and mammalian cells. To investigate the lipid biosynthesis of Euglena gracilis, rapamycin was supplemented in comparison with two model algae, Chlamydomonas reinhardtii and Cyanidioschyzon merolae. In Euglena, rapamycin induced the reduction of chlorophylls and the accumulation of neutral lipids without deterring its cell proliferation. Its lipidomic profile revealed that the fatty acid composition did not alter by supplementing rapamycin. In Chlamydomonas, however, rapamycin induced serious growth inhibition as reported elsewhere. With a lower concentration of rapamycin, the alga accumulated neutral lipids without reducing chlorophylls. In Cyanidioschyzon, rapamycin did not increase neutral lipids but reduced its chlorophyll content. We also tested fatty acid elongase inhibitors such as pyroxasulfone or flufenacet in Euglena with no significant change in its neutral lipid contents. In summary, controlled supplementation of rapamycin can increase the yield of neutral lipids while the scheme is not always applicable for other algal species.
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