Article
Multidisciplinary Sciences
Jekaterina Vohhodina, Liana J. Goehring, Ben Liu, Qing Kong, Vladimir V. Botchkarev, Mai Huynh, Zhiqi Liu, Fieda O. Abderazzaq, Allison P. Clark, Scott B. Ficarro, Jarrod A. Marto, Elodie Hatchi, David M. Livingston
Summary: The research demonstrates that BRCA1 binds TERRA RNA in an R-loop- and cell cycle-dependent manner, represses its transcription, prevents R-loop-associated damage, and promotes repair to maintain telomere stability.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Michal Kroupa, Kristyna Tomasova, Miriam Kavec, Pavel Skrobanek, Tomas Buchler, Rajiv Kumar, Ludmila Vodickova, Pavel Vodicka
Summary: Telomeres are complex protective structures at the ends of linear eukaryotic chromosomes. Telomeric transcripts, especially TERRA, play crucial roles in maintaining genomic stability and gene expression. Understanding the telomere transcriptome is essential for telomere function and human pathology, and may have clinical opportunities in cancer precision medicine.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yajun Wang, Wei Zhu, Yumi Jang, Joshua A. Sommers, Gong Yi, Chandrakala Puligilla, Deborah L. Croteau, Yibin Yang, Mihoko Kai, Yie Liu
Summary: RBM14 is associated with telomeres and negatively regulates TERRA expression, inhibiting the formation of RNA:DNA hybrids at telomeres. It plays a role in protecting telomere integrity in human cancer cells.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Rita Valador Fernandes, Joachim Lingner
Summary: The study reveals the role of THOC in telomeric homeostasis through the co- and post-transcriptional regulation of TERRA R-loops. THOC counteracts the association of TERRA with telomeres by interfering with the formation of R-loops. THOC also promotes telomere stability by suppressing telomeric sister-chromatid exchange and C-circle accumulation in ALT cancer cells.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Caterina Manzato, Luca Larini, Claudio Oss Pegorar, Maria Rosaria Dello Stritto, Katarina Jurikova, Verena Jantsch, Emilio Cusanelli
Summary: This study reports the expression of TERRA in Caenorhabditis elegans. TERRA is regulated by the telomere-binding proteins POT-1 and POT-2, and its transcripts are heterogeneous in length and form discrete nuclear foci. In germ cells, TERRA is expressed in all stages of meiotic prophase I, and it is upregulated prior to activation of the alternative lengthening mechanism (ALT).
NUCLEIC ACIDS RESEARCH
(2023)
Review
Genetics & Heredity
Alain Chebly, Joana Ropio, Lyla Baldasseroni, Martina Prochazkova-Carlotti, Yamina Idrissi, Jacky Ferrer, Chantal Farra, Marie Beylot-Barry, Jean-Philippe Merlio, Edith Chevret
Summary: Telomeric Repeat-containing RNA (TERRA) are long non-coding RNAs transcribed from telomeric DNA sequences and play important roles in various physiological and pathological processes in human cells. This study investigates the involvement of TERRA in cutaneous T-cell lymphomas (CTCL) and suggests a specific role for TERRA 16p downregulation and TERRA 11q upregulation in CTCL lymphomagenesis.
Article
Biochemistry & Molecular Biology
Nikenza Viceconte, Axelle Loriot, Patricia Lona Abreu, Marion Scheibe, Albert Fradera Sola, Falk Butter, Charles De Smet, Claus M. Azzalin, Nausica Arnoult, Anabelle Decottignies
Summary: TERRA molecules play important roles in telomeres, with mouse and human TERRA likely to have similar functions in cells.
Article
Genetics & Heredity
Christopher B. Nelson, Taghreed M. Alturki, Jared J. Luxton, Lynn E. Taylor, David G. Maranon, Keiko Muraki, John P. Murnane, Susan M. Bailey
Summary: The study reveals that telomeric double-strand breaks in human G1 cells activate early DNA damage responses, but do not show localization of important recombination regulator 53BP1 and lack evidence of classical non-homologous end-joining and homologous recombination. Instead, telomeric DSBs in G1 cells lead to extensive tracks of resected 5' C-rich telomeric single-stranded DNA, indicating potential involvement of alternative lengthening of telomere pathway.
FRONTIERS IN GENETICS
(2021)
Article
Biology
Iria Gonzalez-Vasconcellos, Maria A. Cobos-Fernandez, Michael J. Atkinson, Jose Fernandez-Piqueras, Javier Santos
Summary: Telomeric-associated long non-coding RNAs TERRA and TERC can be quantified in culture cell lines and human PBMCs using peptide nucleic acid probes with flow cytometry (RNA-Flow). This method, called RNA-Flow, allows easy and reliable quantification of lncRNAs, with broad applications in basic research and clinical diagnostics. Additionally, the staining protocol presented in this study has been proven useful for detecting and quantifying these lncRNAs on unfixed cells using confocal microscopy.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Arpita Saha, Amit Kumar Gaurav, Unnati M. Pandya, Marjia Afrin, Ranjodh Sandhu, Vishal Nanavaty, Brittny Schnur, Bibo Li
Summary: In this study, several unique features of TERRA and telomere biology in Trypanosoma brucei were identified. It was found that TERRA foci number is cell cycle-regulated and influenced by TbTRF. TbTRF binds TERRA through its C-terminal Myb domain in a sequence-specific manner, suppressing TERRA levels without affecting its half-life, and depletion of TbTRF leads to increased telomeric R-loop and telomere DNA damage.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ru-Xuan Tsai, Kuo-Chen Fang, Po-Cheng Yang, Yu-Hung Hsieh, I-Tien Chiang, Yunfei Chen, Hun-Goo Lee, Jeannie T. Lee, Hsueh-Ping Catherine Chu
Summary: TERRA is an RNA molecule that interacts with the chromatin remodeler ATRX and plays a role in regulating genome stability and transcription. It maintains the stability of DNA G-quadruplex structures and prevents ATRX from binding to subtelomeric regions, thereby repressing H3K9me3 formation. Depletion of TERRA leads to a decrease in G-quadruplex abundance at transcription start sites (TSS), which is associated with increased ATRX occupancy and differential gene expression. Loss of ATRX alleviates the gene repression caused by TERRA depletion, while knockdown of TERRA diminishes DNA G-quadruplex signals and silencing of ATRX increases G-quadruplex formation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Liping Chen, Canfeng Zhang, Wenbin Ma, Junjiu Huang, Yong Zhao, Haiying Liu
Summary: This study reveals the role of m(6)A modification in protecting telomere stability. METTL3 catalyzes m(6)A modification on TERRA, stabilizing its structure and promoting the ALT pathway. Depletion of METTL3 leads to reduced R-loops, telomere shortening, and instability.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Bruno Silva, Rajika Arora, Silvia Bione, Claus M. Azzalin
Summary: TERRA transcription actively destabilizes telomere integrity in ALT cells, thereby triggering BIR and promoting telomere elongation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Woo Suk Choi, Peter J. Weng, Wei Yang
Summary: Telomerase synthesizes telomeres at the ends of linear chromosomes by reverse transcription from a short RNA template. In this study, the potential of the structural cavity of tcTERT to accommodate a looped-out DNA bulge and enable the separation of the RNA/DNA hybrid was investigated. It was found that tcTERT can accommodate and extend a looped-out telomeric repeat in the DNA primer, but retroviral reverse transcriptase cannot. The ternary-complex structure of tcTERT with a downstream RNA/DNA hybrid was also determined, and it was shown to enhance the efficiency of telomere synthesis by tcTERT. Overall, flexible RNA-DNA binding plays a crucial role in telomeric repeat addition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Chih-Yi Gabriela Lin, Anna Christina Naeger, Thomas Lunardi, Aleksandra Vancevska, Gerald Lossaint, Joachim Lingner
Summary: Telomere shortening can lead to diseases and aging, often due to the lack of telomerase in cells. By developing a new isolation protocol, specific proteins associated with telomere replication can be purified, revealing their importance for maintaining telomere stability. This study provides a valuable resource for understanding the dynamic changes of the telomeric proteome during replication.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Obstetrics & Gynecology
Young-Sun Park, Jeong-Kyu Hoh
JOURNAL OF PERINATAL MEDICINE
(2015)
Article
Developmental Biology
Won-Young Choi, Jeong-Kyu Hoh
Article
Obstetrics & Gynecology
Kyu Nam Kim, Young-Sun Park, Jeong-Kyu Hoh
EARLY HUMAN DEVELOPMENT
(2016)
Article
Obstetrics & Gynecology
Jae-Kyoon Hwang, Hyun-Kyung Park, Yoon-Kyoung Sung, Jeong-Kyu Hoh, Hyun Ju Lee
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2018)
Article
Hematology
EunMi Lee, Hye Sook Choi, Jung Hye Hwang, Jeong Kyu Hoh, Youl-Hee Cho, Eun Jung Baek
BLOOD CELLS MOLECULES AND DISEASES
(2014)
Article
Medicine, General & Internal
Hyun Woo Park, Keun Ho Ryu, Yongjun Piao, Peipei Li, Jae Shik Hong, Hee Bum Kim, Hwanwook Chung, Jeong-Kyu Hoh, Yong Joo Kim
JOURNAL OF KOREAN MEDICAL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Mi-Sun Lim, Min-Seop Shin, Soo Young Lee, Yang-Ki Minn, Jeong-Kyu Hoh, Youl-Hee Cho, Dong-Wook Kim, Sang-Hun Lee, Chun-Hyung Kim, Chang-Hwan Park
Article
Gastroenterology & Hepatology
Hyeon Tae Kang, Dae Won Jun, Kiseok Jang, Jeong-Kyu Hoh, Jai Sun Lee, Waqar Khalid Saeed, Yeon Ji Chae, Jin Ho Lee
DIGESTIVE DISEASES AND SCIENCES
(2019)
Review
Developmental Biology
Jeong-Kyu Hoh, Martha Lappas, Calixia Liu, Chong Qiao, Kshetrapal Pallavi, Jun Takeda, Young Ju Kim