期刊
PLANT CELL AND ENVIRONMENT
卷 43, 期 3, 页码 662-674出版社
WILEY
DOI: 10.1111/pce.13691
关键词
Arabidopsis thaliana; drought; eceriferum (cer) mutants; Fourier transform infrared spectroscopy; leaf cuticle
资金
- Global Institute for Food Security (GIFS) through the Plant Phenotyping and Imaging Research Centre (P2IRC) program, at the University of Saskatchewan
- Canada Foundation for Innovation (CFI)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- National Research Council Canada (NRC)
- Canadian Institutes of Health Research (CIHR)
- Government of Saskatchewan
- University of Saskatchewan
Arabidopsis eceriferum (cer) mutants with unique alterations in their rosette leaf cuticular wax accumulation and composition established by gas chromatography have been investigated using attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy in combination with univariate and multivariate analysis. Objectives of this study were to evaluate the utility of ATR-FTIR for detection of chemical diversity in leaf cuticles, obtain spectral profiles of cer mutants in comparison with the wild type, and identify changes in leaf cuticles caused by drought stress. FTIR spectra revealed both genotype- and treatment-dependent differences in the chemical make-up of Arabidopsis leaf cuticles. Drought stress caused specific changes in the integrated area of the CH3 peak, asymmetrical and symmetrical CH2 peaks, ester carbonyl peak and the peak area ratio of ester C(sic)O to CH2 asymmetrical vibration. CH3 peak positively correlated with the total wax accumulation. Thus, ATR-FTIR spectroscopy is a valuable tool that can advance our understanding of the role of cuticle chemistry in plant response to drought and allow selection of superior drought-tolerant varieties from large genetic resources.
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