期刊
BIORESOURCE TECHNOLOGY
卷 213, 期 -, 页码 190-197出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.02.128
关键词
Cyanobacteria; Synechococcus sp PCC 7002; Genome scale metabolic model; Flux Balance Analysis; Minimization of Metabolic Adjustments (MOMA)
资金
- Reliance Industries Ltd., Mumbai
- Department of Biotechnology, Government of India [BT/EB/PANIIT/2012]
Flux Balance Analysis was performed with the Genome Scale Metabolic Model of a fast growing cyanobacterium Synechococcus sp. PCC 7002 to gain insights that would help in engineering the organism as a production host. Gene essentiality and synthetic lethality analysis revealed a reduced metabolic robustness under genetic perturbation compared to the heterotrophic bacteria Escherichia coli. Under glycerol heterotrophy the reducing equivalents were generated from tricarboxylic acid cycle rather than the oxidative pentose phosphate pathway. During mixotrophic growth in glycerol the photosynthetic electron transport chain was predominantly used for ATP synthesis with a photosystem I/photosystem II flux ratio higher than that observed under autotrophy. An exhaustive analysis of all possible double reaction knock outs was performed to reroute fixed carbon towards ethanol and butanol production. It was predicted that only similar to 10% of fixed carbon could be diverted for ethanol and butanol production. (C) 2016 Elsevier Ltd. All rights reserved.
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