The isolation and identification of new microalgal strains producing oil and carotenoid simultaneously with biofuel potential

Taxonomy and phylogeny of twenty two microalgal isolates were examined using both universal and newly designed molecular primers. Among the isolates, Scenedesmus bijugus, Coelastrella sp., Auxenochlorella protothecoides, and Chlorella sp. were particularly promising in terms of producing lipids as measured by fatty acid methyl esters (FAME) analysis and significant concentration of carotenoids. A comparative experiment showed that S. bijugus and Chlorella sp. were the most promising candidates (L-1 d(-1), with biomass) 174.77 +/- 6.75, 169.81 +/- 5.22 mg, lipids 40.14 +/- 3.31, 39.72 +/- 3.89 mg, lutein 0.47, 0.36 mg, and astaxanthin 0.27, 0.18 mg respectively. The fatty acids produced by these microalgal isolates were mainly palmitic, stearic, oleic, linoleic, and linolenic acid. The freshwater microalgal isolate S. bijugus be the most suitable isolate for producing biodiesel and carotenoids, due to high productivity of biomass, lipids, metabolites, and its suitable fatty acid profile. (C) 2016 Elsevier Ltd. All rights reserved.