期刊
BIORESOURCE TECHNOLOGY
卷 218, 期 -, 页码 944-952出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.07.053
关键词
B. subtilis; Surfactin; Medium optimization; Design of experiments; Response surface methodology
资金
- L'Oreal Research and Innovation
- French National Research Agency (ANR) within the frame of the 'Future Investments' program (PIA) [ANR-11-EQPX-0037]
Culture medium elements were analysed by a screening DoE to identify their influence in surfactin specific production by a surfactin constitutive overproducing Bacillus subtilis strain. Statistics pointed the major enhancement caused by high glutamic acid concentrations, as well as a minor positive influence of tryptophan and glucose. Successively, a central composite design was performed in microplate bioreactors using a BioLector((R)), in which variations of these impressive parameters, glucose, glutamic acid and tryptophan concentrations were selected for optimization of product-biomass yield (Y-P/X). Results were exploited in combination with a RSM. In absolute terms, experiments attained an Y-P/X 3.28-fold higher than those obtained in Landy medium, a usual culture medium used for lipopeptide production by B. subtilis. Therefore, two medium compositions for enhancing biomass and surfactin specific production were proposed and tested in continuous regime in a bubbleless membrane bioreactor. An Y-P/X increase of 2.26-fold was observed in bioreactor scale. (C) 2016 Elsevier Ltd. All rights reserved.
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