期刊
NUCLEIC ACIDS RESEARCH
卷 47, 期 20, 页码 10628-10644出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz846
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资金
- Swiss National Science Foundation [31003A 182576]
- University of Geneva
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung [31003A 182576]
- Swiss National Science Foundation (SNF) [31003A_182576] Funding Source: Swiss National Science Foundation (SNF)
Many bacteria acquire dissemination and virulence traits in G1-phase. CtrA, an essential and conserved cell cycle transcriptional regulator identified in the dimorphic alpha-proteobacterium Caulobacter crescentus, first activates promoters in late S-phase and then mysteriously switches to different target promoters in G1-phase. We uncovered a highly conserved determinant in the DNA-binding domain (DBD) of CtrA uncoupling this promoter switch. We also show that it reprograms CtrA occupancy in stationary cells inducing a (p)ppGpp alarmone signal perceived by the RNA polymerase beta subunit. A simple side chain modification in a critical residue within the core DBD imposes opposing developmental phenotypes and transcriptional activities of CtrA and a proximal residue can direct CtrA towards activation of the dispersal (G1-phase) program. Hence, we propose that this conserved determinant in the CtrA primary structure dictates promoter reprogramming during the growth transition in other alpha-proteobacteria that differentiate from replicative cells into dispersal cells.
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