Article
Environmental Sciences
Aigars Lavrinovics, Linda Mezule, Pavels Cacivkins, Talis Juhna
Summary: This study investigated the optimal initial conditions for the growth, phosphate removal, polyphosphate accumulation, and protein productivity of Chlorella vulgaris. Results showed that a 10-day period of phosphate deficiency, accompanied by low initial biomass concentration, significantly increased phosphate removal, polyphosphate accumulation, and protein productivity. The optimization model suggested that one-day biomass P-starvation with low initial biomass concentration was the most effective combination. These findings provide insights into using microalgae exposure to phosphorus stress for rapid phosphorus removal in wastewater treatment.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Microbiology
Francesca Petriglieri, Caitlin M. Singleton, Zivile Kondrotaite, Morten K. D. Dueholm, Elizabeth A. McDaniel, Katherine D. McMahon, Per H. Nielsen
Summary: Candidatus Accumulibacter is an important polyphosphate-accumulating organism (PAO) in wastewater phosphorus removal. However, its taxonomic classification at the species level is complicated. This study provides a comprehensive reassessment of the phylogeny using genome, ppk1, and 16S rRNA gene-based approaches, resulting in the identification of novel species and clarification of the taxonomy. Additionally, the metabolic capabilities of Candidatus Accumulibacter species were explored using genome mining and in situ validation, providing insights into their niche differentiation and contribution to nutrient removal.
Article
Engineering, Environmental
Liping Chen, Gengrui Wei, Yushen Zhang, Kaiying Wang, Cenchao Wang, Xuhan Deng, Yaqian Li, Xiaojing Xie, Jinling Chen, Fu Huang, Hang Chen, Bin Zhang, Chaohai Wei, Guanglei Qiu
Summary: Previous research has shown that Ca. Accumulibacter and Tetrasphaera play cooperative roles in enhanced biological phosphorus removal (EBPR). However, the utilization of fermentation products released by Tetrasphaera and their potential effects on Ca. Accumulibacter metabolism were unknown. This study investigated the metabolic details of Ca. Accumulibacter in using fermentation products for EBPR, providing insights into their interactions.
Article
Agricultural Engineering
Yangang Lin, Yawen Sun, Liyuan Zhang, Qiong Zhang, Xiyao Li, Jun Sui, Yongzhen Peng
Summary: Balancing the removal of nitrogen and phosphorus in municipal wastewater under carbon limitation remains challenging. The key parameter in achieving this balance is the COD-to-PO43--P ratio. By operating an A(2)/O-BCO system for 205 days, it was found that increasing the COD/P ratio deteriorated the nitrogen removal performance, while decreasing it led to the proliferation of Ca.Competibacter and an increase in nitrogen removal. The nitrogen and phosphorus removal efficiency reached up to 79.3% and 95.2%. Overall, establishing a balance between DGAOs and DPAOs through strengthening the effect of DGAOs can improve nutrient removal performance and enhance the stability and efficiency of the system.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Masuduz Zaman, Mingu Kim, George Nakhla
Summary: This study focused on simultaneous nitrification-denitrifying phosphorus removal in a sequencing batch reactor. The process achieved high N and P removal efficiencies in a wide range of COD/N ratios without the addition of volatile fatty acids (VFA). Key factors influencing the process efficiency included COD/N ratio, ffCOD/(TKNoxidized + NOx-N-initial) ratio, and the two-step phosphorus release during the anaerobic period.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Mingchang Gao, Shaofang Sun, Qi Qiu, Weiwei Zhou, Liping Qiu
Summary: This study employed the alternating anoxic-anaerobic/aerobic biofilter system for DPAOs enrichment and successfully achieved simultaneous nitrogen and phosphorus removal from domestic wastewater. Results showed that DPAOs contributed 84% to the overall phosphorus removal after 60 days of operation.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Agricultural Engineering
Hao Zhang, Wei Zhang, Shuang-Shuang Zhang, Wu-Cheng Ma, Liang Zhu, Yi-Ping Li, Yang Pan, Lin Chen
Summary: A novel process for simultaneous denitrification and phosphorus (P) recovery was proposed. The increased nitrate concentration facilitated the activity of denitrifying P removal (DPR) and stimulated P uptake and storage. The total P content in the biofilm and the P concentration in the enriched stream significantly increased. Additionally, the abundance of denitrifying polyphosphate accumulating organisms (DPAOs) increased, and the genes involved in critical functions of metabolism also increased.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Kylie Close, Ricardo Marques, Virginia C. F. Carvalho, Elisabete B. Freitas, Maria A. M. Reis, Gilda Carvalho, Adrian Oehmen
Summary: This study investigates the mechanisms of phosphorus removal, amino acid utilization, and PHA cycling in enriched Tetrasphaera culture. The results suggest that the contribution of Tetrasphaera to phosphorus removal depends on the composition of its Clades. Unlike a previous study with Accumulibacter, the highly enriched Tetrasphaera culture was unable to completely remove phosphorus in SBR cycles.
Article
Ecology
Francesca Petriglieri, Caitlin Singleton, Miriam Peces, Jette F. Petersen, Marta Nierychlo, Per H. Nielsen
Summary: Members of the genus Dechloromonas play a significant role in enhanced biological phosphorus removal systems, with the potential for polyphosphate accumulation. Seven distinct Dechloromonas species were identified in different Danish EBPR plants, each capable of accumulating polyphosphate, glycogen, and polyhydroxyalkanoates.
Article
Ecology
C. M. Singleton, F. Petriglieri, K. Wasmund, M. Nierychlo, Z. Kondrotaite, J. F. Petersen, M. Peces, M. S. Dueholm, M. Wagner, P. H. Nielsen
Summary: The bacterial genus Tetrasphaera is responsible for enhanced biological phosphorus removal (EBPR) in wastewater treatment plants. Recent analyses showed that 16S rRNA genes cannot accurately resolve the lineage of Tetrasphaera, which actually consists of several different genera within the Dermatophilaceae. This study identifies and examines 14 metagenome-assembled genomes containing full-length 16S rRNA genes from wastewater treatment plants, and reveals that the uncultured Tetrasphaera clade 3 represents two distinct genera named Ca. Phosphoribacter and Ca. Lutibacillus. Ca. Phosphoribacter is found to be the most abundant former Tetrasphaera lineage and polyphosphate accumulating organisms (PAO) in EBPR systems in Denmark and globally.
Article
Environmental Sciences
Weihua Zhao, Xuejun Bi, Yongzhen Peng, Meng Bai
Summary: This paper discusses the metabolic mechanisms, process applications, community abundance, and influencing factors of phosphorus-accumulating organisms (PAOs) in wastewater treatment. It identifies novel PAOs, such as Dechloromonas and Tetrasphaera, which can remove phosphorus through different metabolic pathways. Dechloromonas can achieve simultaneous nitrogen and phosphorus removal in an anoxic environment, reducing carbon source requirements and energy consumption.
Article
Engineering, Environmental
Hongwei Sun, Xin Zhang, Feng Zhang, Hao Yang, Jianbo Lu, Shijian Ge, Xiaoqiang Li, Wei Zhang
Summary: The study demonstrates that free ammonia (FA) inhibits phosphorus release and uptake processes in enhanced biological phosphorus removal (EBPR) systems, affecting key microbial communities and Tetrasphaera being identified as the sole virtual biomarker for EBPR performance response to FA inhibition.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Agricultural Engineering
Tiantian Sun, Rui Du, Qiongpeng Dan, Ying Liu, Yongzhen Peng
Summary: This study demonstrated rapid achievement of partial nitrification in an EBPR reactor treating domestic wastewater, leading to stable nitritation within a short period of time. AOB recovered faster than NOB, facilitating the rapid development of PN and significantly increasing both ammonia oxidation rates and nitrite accumulation rates.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Xuhan Deng, Jing Yuan, Liping Chen, Hang Chen, Chaohai Wei, Per H. Nielsen, Stefan Wuertz, Guanglei Qiu
Summary: This study systematically analyzed the occurrence and characteristics of CRISPR-Cas systems and prophages in Ca. Accumulibacter members. The results showed that 28 Ca. Accumulibacter genomes encode CRISPR-Cas systems, providing adaptive resistance to phage predation. The study also revealed high relative abundances of Ca. Accumulibacter with CRISPR-Cas systems in laboratory-scale EBPR bioreactors, indicating their specific resistance to phages.
Article
Engineering, Environmental
Guanglei Qiu, Yingyu Law, Rogelio Zuniga-Montanez, Xuhan Deng, Yang Lu, Samarpita Roy, Sara Swa Thi, Hui Yi Hoon, Thi Quynh Ngoc Nguyen, Kaliyamoorthy Eganathan, Xianghui Liu, Per H. Nielsen, Rohan B. H. Williams, Stefan Wuertz
Summary: Recent research has shown that enhanced biological phosphorus removal (EBPR) from municipal wastewater can be achieved at higher temperatures, around 30 degrees C and 35 degrees C. The study found that a slow-feeding strategy and sufficiently high carbon input are important for maintaining high and stable EBPR performance.
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)